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Maugendre, Laure; Gattuso, Jean-Pierre; Louis, Justine; Anna, de Kluijver; Marro, Sophie; Soetaert, Karline; Gazeau, Frédéric; Dufour, Aurélie (2015): Villefranche sur Mer: multistressors experiment March 2012. PANGAEA,, Supplement to: Maugendre, Laure; Gattuso, Jean-Pierre; Louis, Justine; de Kluijver, Anna; Marro, Sophie; Soetaert, Karline; Gazeau, Frédéric (2014): Effect of ocean warming and acidification on a plankton community in the NW Mediterranean Sea. ICES Journal of Marine Science,

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The effect of ocean warming and acidification was investigated on a natural plankton assemblage from an oligotrophic area, the bay of Villefranche (NW Mediterranean Sea). The assemblage was sampled in March 2012 and exposed to the following four treatments for 12 days: control ( 360 µatm, 14°C), elevated pCO2 ( 610 µatm, 14°C), elevated temperature ( 410 µatm, 17°C), and elevated pCO2 and temperature ( 690 µatm, 17°C). Nutrients were already depleted at the beginning of the experiment and the concentrations of chlorophyll a (chl a), heterotrophic prokaryotes and viruses decreased, under all treatments, throughout the experiment. There were no statistically significant effects of ocean warming and acidification, whether in isolation or combined, on the concentrations of nutrients, particulate organic matter, chl a and most of the photosynthetic pigments. Furthermore, 13C labelling showed that the carbon transfer rates from 13C-sodium bicarbonate into particulate organic carbon were not affected by seawater warming nor acidification. Rates of gross primary production followed the general decreasing trend of chl a concentrations and were significantly higher under elevated temperature, an effect exacerbated when combined to elevated pCO2 level. In contrast to the other algal groups, the picophytoplankton population (cyanobacteria, mostly Synechococcus) increased throughout the experiment and was more abundant in the warmer treatment though to a lesser extent when combined to high pCO2 level. These results suggest that under nutrient-depleted conditions in the Mediterranean Sea, ocean acidification has a very limited impact on the plankton community and that small species will benefit from warming with a potential decrease of the export and energy transfer to higher trophic levels.
Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Entire community; Laboratory experiment; Mediterranean Sea; Pelagos; Primary production/Photosynthesis; Respiration; Temperate; Temperature
Further details:
Lavigne, Héloise; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0.
Seventh Framework Programme (FP7), grant/award no. 265103: Mediterranean Sea Acidification in a Changing Climate
Latitude: 43.400000 * Longitude: 7.180000
Date/Time Start: 2012-03-14T08:00:00 * Date/Time End: 2012-03-24T06:00:00
Villefranche-2012 * Latitude: 43.400000 * Longitude: 7.180000 * Date/Time Start: 2012-03-14T07:30:00 * Date/Time End: 2012-03-27T12:32:00 * Method/Device: Experiment (EXP)
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-07-22.
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Other eventOther eventMaugendre, Laure
2TreatmentTreatMaugendre, LaureC = control; OW = Ocean warming; OA=Ocean acidfication and G = greenhouse
3Day of experimentDOEdayMaugendre, Laure
5Bottle numberBottleMaugendre, Laure
6Temperature, waterTemp°CMaugendre, Laure
7OxygenO2µmol/lMaugendre, LaureTitration, Winkler
8IrradianceEµmol/m2/sMaugendre, Laure
9SalinitySalMaugendre, Laure
10Carbon, inorganic, dissolvedDICµmol/kgMaugendre, LaureCoulometric titration
11Alkalinity, totalATµmol/kgMaugendre, LaurePotentiometric titration
12Carbonate system computation flagCSC flagMaugendre, LaureCalculated using seacarb
13pHpHMaugendre, LaureCalculated using seacarbtotal scale
14Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmMaugendre, LaureCalculated using seacarb
15Bicarbonate ion[HCO3]-µmol/kgMaugendre, LaureCalculated using seacarb
16Carbonate ion[CO3]2-µmol/kgMaugendre, LaureCalculated using seacarb
17Aragonite saturation stateOmega ArgMaugendre, LaureCalculated using seacarb
18Calcite saturation stateOmega CalMaugendre, LaureCalculated using seacarb
19Carbon, organic, dissolvedDOCµmol/lMaugendre, Laure
20Nitrogen, inorganic, dissolvedDINµmol/lLouis, JustineSpectrophotometric
21Phosphorus, inorganic, dissolvedDIPµmol/lLouis, JustineSpectrophotometric
22SilicateSi(OH)4µmol/lDufour, AurélieSpectrophotometric
23Nitrogen, organic, particulatePONµmol/lMaugendre, LaureElement analyser CHN
24Carbon, organic, particulatePOCµmol/lMaugendre, LaureElement analyser CHN
25Chlorophyll c3Chl c3µg/lMaugendre, LaureHigh Performance Liquid Chromatography (HPLC)
26Chlorophyll c1+c2Chl c1+c2µg/lMaugendre, LaureHigh Performance Liquid Chromatography (HPLC)
27PeridininPeridµg/lMaugendre, LaureHigh Performance Liquid Chromatography (HPLC)
2819-ButanoyloxyfucoxanthinBut-fucoµg/lMaugendre, LaureHigh Performance Liquid Chromatography (HPLC)
29FucoxanthinFucoµg/lMaugendre, LaureHigh Performance Liquid Chromatography (HPLC)
30NeoxanthinNeoµg/lMaugendre, LaureHigh Performance Liquid Chromatography (HPLC)
31PrasinoxanthinPrasµg/lMaugendre, LaureHigh Performance Liquid Chromatography (HPLC)
32ViolaxanthinViolaµg/lMaugendre, LaureHigh Performance Liquid Chromatography (HPLC)
3319-HexanoyloxyfucoxanthinHex-fucoµg/lMaugendre, LaureHigh Performance Liquid Chromatography (HPLC)
34DiadinoxanthinDiadinoµg/lMaugendre, LaureHigh Performance Liquid Chromatography (HPLC)
35AlloxanthinAlloµg/lMaugendre, LaureHigh Performance Liquid Chromatography (HPLC)
36DiatoxanthinDiatoµg/lMaugendre, LaureHigh Performance Liquid Chromatography (HPLC)
37ZeaxanthinZeaµg/lMaugendre, LaureHigh Performance Liquid Chromatography (HPLC)
38Chlorophyll bChl bµg/lMaugendre, LaureHigh Performance Liquid Chromatography (HPLC)
39Chlorophyll aChl aµg/lMaugendre, LaureHigh Performance Liquid Chromatography (HPLC)
40Phaeophytin aPhaeophytin aµg/lMaugendre, LaureHigh Performance Liquid Chromatography (HPLC)
41CaroteneCaroteneµg/lMaugendre, LaureHigh Performance Liquid Chromatography (HPLC)
42VirusVirus#/mlMarro, SophieFlow cytometry
43Virus, low DNA fluorescenceVirus LDNA#/mlMarro, SophieFlow cytometry
44Virus, medium DNA fluorescenceVirus HDNA#/mlMarro, SophieFlow cytometry
45Virus, high DNA fluorescenceVirus HDNA#/mlMarro, SophieFlow cytometry
46SynechococcusSynechococcus#/mlMarro, SophieFlow cytometry
47Picoeukaryotes, autotrophicPEuk auto#/mlMarro, SophieFlow cytometry
48BacteriaBact#/mlMarro, SophieFlow cytometry
49ProchlorococcusProchlorococcus#/mlMarro, SophieFlow cytometry
50NanoprokaryotesNprok#/mlMarro, SophieFlow cytometry
51Net community production of oxygenNCP O2µmol/l/dayMaugendre, Laure
52Net community production of oxygen, standard errorNCP std e±Maugendre, Laure
53Respiration rate, oxygenResp O2µmol/l/dayMaugendre, Laure
54Respiration rate, oxygen, standard errorResp O2 std e±Maugendre, Laure
55Gross community production of oxygenGCPOµmol/l/dayMaugendre, Laure
56Gross community production of oxygen, standard errorGCPO std e±Maugendre, Laure
57Gross primary production, 18OGPP 18Oµmol/l/dayMaugendre, Laure
58Net community production of carbon dioxideNCP CO2µmol/l/dayMaugendre, Laure
59δ13C, dissolved inorganic carbonδ13C DIC‰ PDBMaugendre, Laure
60Δδ13CΔδ13C‰ PDBMaugendre, LaureDIC
61δ13C, particulate organic carbonδ13C POC‰ PDBMaugendre, Laure
62Δδ13CΔδ13C‰ PDBMaugendre, LaurePOC
63Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
64pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
65Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
66Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
67Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
68Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
69Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
70Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
71Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
3443 data points

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