De Vittor Cinzia cdevittor@ogs.it 0000-0001-8552-4396 Cerino Federica 0000-0002-9191-9957 Coscia Lucia Beran Alfred Kralj Martina mkralj@ogs.trieste.it 0000-0003-1544-5515 Comici Cinzia ccomici@ogs.trieste.it 0000-0002-9431-8495 Karuza Ana akaruza@ogs.trieste.it 2014 Culture experiments to evaluate CO2 effects on growth and morphology of the coccolithophore Pleurochrysis cf. pseudoroscoffensis dataset Istituto Nazionale di Oceanografia e di Geofisica Sperimentale, Sgonico https://doi.org/10.1594/PANGAEA.833845 2014-07-08T08:12:14 In the framework of the Italian project CO2 Monitor, two culture experiments were carried out in vertical closed photobioreactors with Pleurochrysis cf. pseudoroscoffensis Gayral & Fresnel 1983, a coccolithophore isolated from the Gulf of Trieste (North Adriatic Sea). The aim of this study was to investigate the effects induced by pH variations due to CO2 emissions on its growth and morphology. Two experiments were carried out with two different CO2 concentrations (1 and 2%) relatively to the total volume of insufflated air. Growth and cell size in light microscopy, morphology and coccolith size in scanning electron microscopy, particulate nitrogen (PN) and particulate inorganic and organic carbon (PIC and POC) content of the coccolithophore were investigated during the light and dark phases. Dissolved inorganic nutrient (nitrate and phosphate) concentrations and pH of the medium and the presence of heterotrophic prokaryotes (HP) were monitored as well. Scanning electron micrographs of Pleurocrhysis cf. pseudoroscoffensis other hdl:10013/epic.43898.d001 2012-11-19T12:00:00 2013-06-19T07:00:00 point ECO2 Sub-seabed CO2 Storage: Impact on Marine Ecosystems http://www.eco2-project.eu/ IFM-GEOMAR Leibniz-Institute of Marine Sciences, Kiel University IFM-GEOMAR http://www.geomar.de https://ror.org/02h2x0161 Seventh Framework Programme of the European Commission FP7 https://doi.org/10.13039/100011102 265847 Sub-seabed CO2 Storage: Impact on Marine Ecosystems https://cordis.europa.eu/project/id/265847 750 CC-BY-3.0 Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/ Slight changes in morphology of coccoliths resulted from analysis of scanning electron micrographs. Coccoliths particularly showed thinner inner tubes and a lower number of nodes compared to the control in the experiment with 1% CO2. Treatment Treat treatment De Vittor Cinzia cdevittor@ogs.it 0000-0001-8552-4396 Treat DATE/TIME Date/Time http://en.wikipedia.org/wiki/ISO_8601 Time T http://dbpedia.org/resource/Time De Vittor Cinzia cdevittor@ogs.it 0000-0001-8552-4396 Date/Time Day of experiment DOE day day Time T http://dbpedia.org/resource/Time De Vittor Cinzia cdevittor@ogs.it 0000-0001-8552-4396 DOE [day] Species Species https://en.wikipedia.org/wiki/Species species De Vittor Cinzia cdevittor@ogs.it 0000-0001-8552-4396 Species Cell Cell #/ml cell De Vittor Cinzia cdevittor@ogs.it 0000-0001-8552-4396 Cell [#/ml] Length l µm Length L http://dbpedia.org/resource/Length De Vittor Cinzia cdevittor@ogs.it 0000-0001-8552-4396 coccolith l [µm] (coccolith) Width w µm width http://purl.obolibrary.org/obo/PATO_0000921 Length L http://dbpedia.org/resource/Length De Vittor Cinzia cdevittor@ogs.it 0000-0001-8552-4396 coccolith w [µm] (coccolith) Length l µm Length L http://dbpedia.org/resource/Length De Vittor Cinzia cdevittor@ogs.it 0000-0001-8552-4396 cell l [µm] (cell) Width w µm width http://purl.obolibrary.org/obo/PATO_0000921 Length L http://dbpedia.org/resource/Length De Vittor Cinzia cdevittor@ogs.it 0000-0001-8552-4396 cell w [µm] (cell) pH pH pH meter Chemical property sensors pH sensors http://vocab.nerc.ac.uk/collection/L05/current/355/ De Vittor Cinzia cdevittor@ogs.it 0000-0001-8552-4396 pH Nitrogen, total, particulate TPN µmol/l nitrogen N https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:25555 Molar Concentration De Vittor Cinzia cdevittor@ogs.it 0000-0001-8552-4396 TPN [µmol/l] Carbon, organic, particulate POC µmol/l carbon C https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:27594 organic particulate Molar Concentration De Vittor Cinzia cdevittor@ogs.it 0000-0001-8552-4396 POC [µmol/l] Carbon, inorganic, particulate PIC µmol/l carbon C https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:27594 particulate Molar Concentration De Vittor Cinzia cdevittor@ogs.it 0000-0001-8552-4396 PIC [µmol/l] Ammonium [NH4]+ µmol/l ammonium http://purl.obolibrary.org/obo/CHEBI_28938 Molar Concentration De Vittor Cinzia cdevittor@ogs.it 0000-0001-8552-4396 [NH4]+ [µmol/l] Nitrate [NO3]- µmol/l nitrate http://purl.obolibrary.org/obo/CHEBI_17632 Molar Concentration De Vittor Cinzia cdevittor@ogs.it 0000-0001-8552-4396 [NO3]- [µmol/l] Nitrite [NO2]- µmol/l nitrite http://purl.obolibrary.org/obo/CHEBI_16301 Molar Concentration De Vittor Cinzia cdevittor@ogs.it 0000-0001-8552-4396 [NO2]- [µmol/l] Phosphate [PO4]3- µmol/l phosphate http://purl.obolibrary.org/obo/CHEBI_26020 Molar Concentration De Vittor Cinzia cdevittor@ogs.it 0000-0001-8552-4396 [PO4]3- [µmol/l] Silicon dioxide SiO2 µmol/l https://en.wikipedia.org/wiki/Silicon_dioxide silicon dioxide http://purl.obolibrary.org/obo/CHEBI_30563 Molar Concentration De Vittor Cinzia cdevittor@ogs.it 0000-0001-8552-4396 SiO2 [µmol/l] Prokaryotes, heterotroph Prok het #/ml De Vittor Cinzia cdevittor@ogs.it 0000-0001-8552-4396 Prok het [#/ml] citable author32892 author41052 author41054 author47905 author49712 author52164 author52165 award52 funder20486 geocode1599 inst6 license101 method6970 param128779 param18664 param18941 param21033 param29999 param52 param7053 param756 param757 param758 param764 param7646 param7647 param78394 param80002 param96278 pi41052 project4138 ref64714 sourceinst2005 term1073173 term1073224 term1073290 term1073482 term1073483 term1073484 term2299532 term38338 term38477 term38548 term40551 term41013 term43863 term43975 term44030 term44419 term48982 term49384 term67625 topotype2