PANGAEA_833845 Culture experiments to evaluate CO2 effects on growth and morphology of the coccolithophore Pleurochrysis cf. pseudoroscoffensis De Vittor, Cinzia; Cerino, Federica; Coscia, Lucia; Beran, Alfred; Kralj, Martina; Comici, Cinzia; Karuza, Ana Culture experiments to evaluate CO2 effects on growth and morphology of the coccolithophore Pleurochrysis cf. pseudoroscoffensis 2014-07-08 PANGAEA Dataset https://doi.pangaea.de/10.1594/PANGAEA.833845 Investigator Cinzia De Vittor cdevittor@ogs.it Earth Science Treatment DATE/TIME Day of experiment Species Cell Length Width pH Nitrogen, total, particulate Carbon, organic, particulate Carbon, inorganic, particulate Ammonium Nitrate Nitrite Phosphate Silicon dioxide Prokaryotes, heterotroph geoscientificInformation pH meter 2012-11-19 2013-06-19 Complete ECO2 Sub-seabed CO2 Storage: Impact on Marine Ecosystems unrestricted CC-BY-3.0: Creative Commons Attribution 3.0 Unported English Istituto Nazionale di Oceanografia e di Geofisica Sperimentale, Sgonico PANGAEA Data Publisher for Earth & Environmental Science https://www.pangaea.de/ Data Center Contact Michael Diepenbroek info@pangaea.de

Leobener Str.

Bremen Bremen 28359 Germany online 750 data points text/tab-separated-values Scanning electron micrographs of Pleurocrhysis cf. pseudoroscoffensis. hdl:10013/epic.43898.d001

In the framework of the Italian project CO2 Monitor, two culture experiments were carried out in vertical closed photobioreactors with Pleurochrysis cf. pseudoroscoffensis Gayral & Fresnel 1983, a coccolithophore isolated from the Gulf of Trieste (North Adriatic Sea). The aim of this study was to investigate the effects induced by pH variations due to CO2 emissions on its growth and morphology. Two experiments were carried out with two different CO2 concentrations (1 and 2%) relatively to the total volume of insufflated air. Growth and cell size in light microscopy, morphology and coccolith size in scanning electron microscopy, particulate nitrogen (PN) and particulate inorganic and organic carbon (PIC and POC) content of the coccolithophore were investigated during the light and dark phases. Dissolved inorganic nutrient (nitrate and phosphate) concentrations and pH of the medium and the presence of heterotrophic prokaryotes (HP) were monitored as well. ** For all details see the full metadata description at "https://doi.pangaea.de/10.1594/PANGAEA.833845"! ** Slight changes in morphology of coccoliths resulted from analysis of scanning electron micrographs. Coccoliths particularly showed thinner inner tubes and a lower number of nodes compared to the control in the experiment with 1% CO2.

http://en.wikipedia.org/wiki/ISO_8601 DATE/TIME http://www.eco2-project.eu/ ECO2 https://en.wikipedia.org/wiki/Silicon_dioxide Silicon dioxide https://en.wikipedia.org/wiki/Species Species https://hdl.handle.net/10013/epic.43898.d001 Scanning electron micrographs of Pleurocrhysis cf. pseudoroscoffensis DIF 9.4 2014-07-08 2026-06-01