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Hans, Stephanie; Fehsenfeld, Sandra; Treberg, Jason R; Weihrauch, Dirk (2014): Acid-base regulation in the Dungeness crab (Metacarcinus magister). PANGAEA, https://doi.org/10.1594/PANGAEA.833716, Supplement to: Hans, S et al. (2014): Acid–base regulation in the Dungeness crab (Metacarcinus magister). Marine Biology, 161(5), 1179-1193, https://doi.org/10.1007/s00227-014-2409-7

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Abstract:
Homeostatic regulation allows organisms to secure basic physiological processes in a varying environment. To counteract fluctuations in ambient carbonate system speciation due to elevated seawater pCO2 (hypercapnia), many aquatic crustaceans excrete/accumulate acid-base equivalents through their gills; however, not much is known about the role of ammonia in this response. The present study investigated the effects of hypercapnia on acid-base and ammonia regulation in the Dungeness crab, Metacarcinus magister on the whole animal and isolated gill levels. Hemolymph pCO2 and [HCO3]- increased in M. magister acclimated to elevated pCO2 (330 Pa), while pH remained stable. Additionally, hemolymph [Na+], [Ca2+], and [SO4]2- were significantly increased. When challenged with varying pH during gill perfusion, the pH of the artificial hemolymph remained relatively unchanged. Overall, ammonia production and excretion, as well as oxygen consumption, were reduced in crabs acclimated to elevated pCO2, demonstrating that either (amino acid) oxidation is reduced in response to this particular stress, or nitrogenous wastes are excreted in an alternative form.
Further details:
Lavigne, Héloise; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-07-01.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1SpeciesSpeciesFehsenfeld, Sandra
2FigureFigFehsenfeld, Sandra
3TableTabFehsenfeld, Sandra
4TreatmentTreatFehsenfeld, Sandra
5TreatmentTreatFehsenfeld, Sandrabath solution
6IdentificationIDFehsenfeld, Sandracrab
7Haemolymph, pHpH (ha)Fehsenfeld, Sandra
8Haemolymph, partial pressure of carbon dioxidepCO2 (ha)kPaFehsenfeld, Sandra
9Haemolymph, bicarbonate ion[HCO3]- (ha)mmol/lFehsenfeld, Sandra
10Ammonia excretionNH3/[NH4]+ excnmol/g/hFehsenfeld, Sandrawhole-animal
11Time point, descriptiveTime pointFehsenfeld, Sandra
12Respiration rate, oxygenResp O2µmol/g/hFehsenfeld, Sandra
13ReplicatesRepl#Fehsenfeld, Sandra
14pHpHFehsenfeld, Sandraperfusate, NBS scale
15Sample code/labelSample labelFehsenfeld, Sandra
16Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetPaFehsenfeld, Sandrachange in perfusate pCO2
17Bicarbonate ion increase rate[HCO3]- inc ratemmol/g/hFehsenfeld, Sandra
18Ammonia excretionNH3/[NH4]+ excµmol/g/hFehsenfeld, Sandrabranchial
19Ammonia excretionNH3/[NH4]+ excµmol/g/hFehsenfeld, Sandrabranchial metabolic
20HemocyaninHemocyaninmg/mlFehsenfeld, Sandra
21Ammonia+AmmoniumNH3+[NH4]+µmol/lFehsenfeld, Sandrahemolymph
22Sodium ionNa+mmol/lFehsenfeld, Sandrahemolymph
23Potassium ionK+mmol/lFehsenfeld, Sandrahemolymph
24Calcium ionCa2+mmol/lFehsenfeld, Sandrahemolymph
25Magnesium ionMg2+mmol/lFehsenfeld, Sandrahemolymph
26Chloride ionCl-mmol/lFehsenfeld, Sandrahemolymph
27Sulfate ion[SO4]2-mmol/lFehsenfeld, Sandrahemolymph
28OsmolalityOsmmOsm/kgFehsenfeld, Sandrahemolymph
29SalinitySalFehsenfeld, Sandra
30Temperature, waterTemp°CFehsenfeld, Sandra
31Carbon, inorganic, dissolvedDICµmol/kgFehsenfeld, SandraCoulometric titration
32Carbon, inorganic, dissolved, standard errorDIC std e±Fehsenfeld, SandraCoulometric titration
33Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetPaFehsenfeld, SandraCalculated using CO2SYS
34Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard errorpCO2water_SST_wet std e±Fehsenfeld, SandraCalculated using CO2SYS
35Bicarbonate ion[HCO3]-µmol/kgFehsenfeld, SandraCalculated using CO2SYS
36Bicarbonate ion, standard error[HCO3]- std e±Fehsenfeld, SandraCalculated using CO2SYS
37Alkalinity, totalATµmol/kgFehsenfeld, SandraCalculated using CO2SYS
38Alkalinity, total, standard errorAT std e±Fehsenfeld, SandraCalculated using CO2SYS
39pHpHFehsenfeld, SandraPotentiometricNBS scale
40pH, standard errorpH std e±Fehsenfeld, SandraPotentiometricNBS scale
41Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
42pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
43Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
44Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
45Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
46Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
47Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
48Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
49Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Size:
9739 data points

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