Citation:

s00227-014-2409-7

Name: Acid-base regulation in the Dungeness crab (Metacarcinus magister)
Published: 2014-07-01
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Acid-base regulation; Animalia; Arthropoda; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Metacarcinus magister; Not applicable; Other metabolic rates; Respiration; Single species; Temperate

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Abstract:

Homeostatic regulation allows organisms to secure basic physiological processes in a varying environment. To counteract fluctuations in ambient carbonate system speciation due to elevated seawater pCO2 (hypercapnia), many aquatic crustaceans excrete/accumulate acid-base equivalents through their gills; however, not much is known about the role of ammonia in this response. The present study investigated the effects of hypercapnia on acid-base and ammonia regulation in the Dungeness crab, Metacarcinus magister on the whole animal and isolated gill levels. Hemolymph pCO2 and [HCO3]- increased in M. magister acclimated to elevated pCO2 (330 Pa), while pH remained stable. Additionally, hemolymph [Na+], [Ca2+], and [SO4]2- were significantly increased. When challenged with varying pH during gill perfusion, the pH of the artificial hemolymph remained relatively unchanged. Overall, ammonia production and excretion, as well as oxygen consumption, were reduced in crabs acclimated to elevated pCO2, demonstrating that either (amino acid) oxidation is reduced in response to this particular stress, or nitrogenous wastes are excreted in an alternative form.

Keyword(s):

Acid-base regulation; Animalia; Arthropoda; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Metacarcinus magister; Not applicable; Other metabolic rates; Respiration; Single species; Temperate

Further details:

Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-07-01.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Fehsenfeld, Sandra
2	Figure	Fig		Fehsenfeld, Sandra
3	Table	Tab		Fehsenfeld, Sandra
4	Treatment	Treat		Fehsenfeld, Sandra
5	Treatment	Treat		Fehsenfeld, Sandra		bath solution
6	Identification	ID		Fehsenfeld, Sandra		crab
7	Haemolymph, pH	pH (ha)		Fehsenfeld, Sandra
8	Haemolymph, partial pressure of carbon dioxide	pCO2 (ha)	kPa	Fehsenfeld, Sandra
9	Haemolymph, bicarbonate ion	[HCO3]- (ha)	mmol/l	Fehsenfeld, Sandra
10	Ammonia excretion	NH3/[NH4]+ exc	nmol/g/h	Fehsenfeld, Sandra		whole-animal
11	Time point, descriptive	Time point		Fehsenfeld, Sandra
12	Respiration rate, oxygen	Resp O2	µmol/g/h	Fehsenfeld, Sandra
13	Replicates	Repl	#	Fehsenfeld, Sandra
14	pH	pH		Fehsenfeld, Sandra		perfusate, NBS scale
15	Sample code/label	Sample label		Fehsenfeld, Sandra
16	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	Pa	Fehsenfeld, Sandra		change in perfusate pCO2
17	Bicarbonate ion increase rate	[HCO3]- inc rate	mmol/g/h	Fehsenfeld, Sandra
18	Ammonia excretion	NH3/[NH4]+ exc	µmol/g/h	Fehsenfeld, Sandra		branchial
19	Ammonia excretion	NH3/[NH4]+ exc	µmol/g/h	Fehsenfeld, Sandra		branchial metabolic
20	Hemocyanin	Hemocyanin	mg/ml	Fehsenfeld, Sandra
21	Ammonia+Ammonium	NH3 + [NH4]+	µmol/l	Fehsenfeld, Sandra		hemolymph
22	Sodium ion	Na+	mmol/l	Fehsenfeld, Sandra		hemolymph
23	Potassium ion	K+	mmol/l	Fehsenfeld, Sandra		hemolymph
24	Calcium ion	Ca2+	mmol/l	Fehsenfeld, Sandra		hemolymph
25	Magnesium ion	Mg2+	mmol/l	Fehsenfeld, Sandra		hemolymph
26	Chloride ion	Cl-	mmol/l	Fehsenfeld, Sandra		hemolymph
27	Sulfate ion	[SO4]2-	mmol/l	Fehsenfeld, Sandra		hemolymph
28	Osmolality	Osm	mOsm/kg	Fehsenfeld, Sandra		hemolymph
29	Salinity	Sal		Fehsenfeld, Sandra
30	Temperature, water	Temp	°C	Fehsenfeld, Sandra
31	Carbon, inorganic, dissolved	DIC	µmol/kg	Fehsenfeld, Sandra	Coulometric titration
32	Carbon, inorganic, dissolved, standard error	DIC std e	±	Fehsenfeld, Sandra	Coulometric titration
33	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	Pa	Fehsenfeld, Sandra	Calculated using CO2SYS
34	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error	pCO2water_SST_wet std e	±	Fehsenfeld, Sandra	Calculated using CO2SYS
35	Bicarbonate ion	[HCO3]-	µmol/kg	Fehsenfeld, Sandra	Calculated using CO2SYS
36	Bicarbonate ion, standard error	[HCO3]- std e	±	Fehsenfeld, Sandra	Calculated using CO2SYS
37	Alkalinity, total	AT	µmol/kg	Fehsenfeld, Sandra	Calculated using CO2SYS
38	Alkalinity, total, standard error	AT std e	±	Fehsenfeld, Sandra	Calculated using CO2SYS
39	pH	pH		Fehsenfeld, Sandra	Potentiometric	NBS scale
40	pH, standard error	pH std e	±	Fehsenfeld, Sandra	Potentiometric	NBS scale
41	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
43	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

9739 data points

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