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Trimborn, Scarlett; Thoms, Silke; Petrou, Katherina; Kranz, Sven A; Rost, Bjoern (2014): Photophysiological responses of Southern Ocean phytoplankton to changes in CO2 concentrations: Short-term versus acclimation effects. PANGAEA, https://doi.org/10.1594/PANGAEA.833713, Supplement to: Trimborn, S et al. (2014): Photophysiological responses of Southern Ocean phytoplankton to changes in CO2 concentrations: Short-term versus acclimation effects. Journal of Experimental Marine Biology and Ecology, 451, 44-54, https://doi.org/10.1016/j.jembe.2013.11.001

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Abstract:
The present study examines how different pCO2 acclimations affect the CO2- and light-dependence of photophysiological processes and O2 fluxes in four Southern Ocean (SO) key phytoplankton species. We grew Chaetoceros debilis (Cleve), Pseudo-nitzschia subcurvata (Hasle), Fragilariopsis kerguelensis (O'Meara) and Phaeocystis antarctica (Karsten) under low (160 µatm) and high (1000 ?atm) pCO2. The CO2- and light-dependence of fluorescence parameters of photosystem II (PSII) were determined by means of a fluorescence induction relaxation system (FIRe). In all tested species, nonphotochemical quenching (NPQ) is the primary photoprotection strategy in response to short-term exposure to high light or low CO2 concentrations. In C. debilis and P. subcurvata, PSII connectivity (p) and functional absorption cross-sections of PSII in ambient light (sigma PSII') also contributed to photoprotection while changes in re-oxidation times of Qa acceptor (tQa) were more significant in F. kerguelensis. The latter was also the only species being responsive to high acclimation pCO2, as these cells had enhanced relative electron transport rates (rETRs) and sigma PSII' while tQa and p were reduced under short-term exposure to high irradiance. Low CO2-acclimated cells of F. kerguelensis and all pCO2 acclimations of C. debilis and P. subcurvata showed dynamic photoinhibition with increasing irradiance. To test for the role and presence of the Mehler reaction in C. debilis and P. subcurvata, the light-dependence of O2 fluxes was estimated using membrane inlet mass spectrometry (MIMS). Our results show that the Mehler reaction is absent in both species under the tested conditions. We also observed that dark respiration was strongly reduced under high pCO2 in C. debilis while it remained unaltered in P. subcurvata. Our study revealed species-specific differences in the photophysiological responses to pCO2, both on the acclimation as well as the short-term level.
Further details:
Lavigne, Héloise; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-07-01.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1FigureFigTrimborn, Scarlett
2SpeciesSpeciesTrimborn, Scarlett
3Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmTrimborn, Scarletttreatment
4TreatmentTreatTrimborn, Scarlett
5Carbon dioxideCO2µmol/lTrimborn, Scarlett
6IrradianceEµmol/m2/sTrimborn, Scarlett
7Effective absorbance cross-section of photosystem IIsigma PSIIA2/quantaTrimborn, Scarlett
8Non photochemical quenchingNPQTrimborn, Scarlett
9Electron transport rate, relativerETRµmol e/m2/sTrimborn, Scarlett
10Effective quantum yieldYTrimborn, Scarlett
11Re-oxidation time of the Qa acceptortQaµsTrimborn, Scarlettin ambient light, CO2-dependence
12Re-oxidation time of the Qa acceptor, standard deviationtQa std dev±Trimborn, Scarlettin ambient light, CO2-dependence
13Re-oxidation time of the Qa acceptortQaµsTrimborn, ScarlettCO2-dependence
14Re-oxidation time of the Qa acceptor, standard deviationtQa std dev±Trimborn, ScarlettCO2-dependence
15Re-oxidation time of the Qa acceptortQaµsTrimborn, Scarlettin ambient light, light-dependence
16Re-oxidation time of the Qa acceptor, standard deviationtQa std dev±Trimborn, Scarlettin ambient light, light-dependence
17Re-oxidation time of the Qa acceptortQaµsTrimborn, Scarlettlight-dependence
18Re-oxidation time of the Qa acceptor, standard deviationtQa std dev±Trimborn, Scarlettlight-dependence
19Connectivity between photosystem IIpTrimborn, Scarlettin ambient light, CO2-dependence
20Connectivity between photosystem II, standard deviationp std dev±Trimborn, Scarlettin ambient light, CO2-dependence
21Connectivity between photosystem IIpTrimborn, ScarlettCO2-dependence
22Connectivity between photosystem II, standard deviationp std dev±Trimborn, ScarlettCO2-dependence
23Connectivity between photosystem IIpTrimborn, Scarlettin ambient light, light-dependence
24Connectivity between photosystem II, standard deviationp std dev±Trimborn, Scarlettin ambient light, light-dependence
25Connectivity between photosystem IIpTrimborn, Scarlettlight-dependence
26Connectivity between photosystem II, standard deviationp std dev±Trimborn, Scarlettlight-dependence
27TableTabTrimborn, Scarlett
28Gross oxygen evolution, per chlorophyll aO2 ev/Chlµmol/mg/hTrimborn, Scarlett
29Gross oxygen evolution, standard deviationO2 ev/Chl std dev±Trimborn, Scarlett
30Net oxygen evolution, per chlorophyll aO2 ev/Chlµmol/mg/hTrimborn, Scarlett
31Net oxygen evolution, per chlorophyll a, standard deviationO2 ev/Chl std dev±Trimborn, Scarlett
32Respiration rate, oxygenResp O2µmol/mg/hTrimborn, Scarlettper Chl a, in the light
33Respiration rate, oxygen, standard deviationResp O2 std dev±Trimborn, Scarlettper Chl a, in the light
34Respiration rate, oxygenResp O2µmol/mg/hTrimborn, Scarlettper Chl a, in the dark
35Respiration rate, oxygen, standard deviationResp O2 std dev±Trimborn, Scarlettper Chl a, in the dark
36Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmTrimborn, ScarlettCalculated using CO2SYS
37Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Trimborn, ScarlettCalculated using CO2SYS
38Carbon dioxideCO2µmol/kgTrimborn, ScarlettCalculated using CO2SYS
39Carbon dioxide, standard deviationCO2 std dev±Trimborn, ScarlettCalculated using CO2SYS
40Carbon, inorganic, dissolvedDICµmol/kgTrimborn, ScarlettCalculated using CO2SYS
41Carbon, inorganic, dissolved, standard deviationDIC std dev±Trimborn, ScarlettCalculated using CO2SYS
42Alkalinity, totalATµmol/kgTrimborn, ScarlettPotentiometric titration
43Alkalinity, total, standard deviationAT std dev±Trimborn, ScarlettPotentiometric titration
44pHpHTrimborn, ScarlettPotentiometricNBS scale
45pH, standard deviationpH std dev±Trimborn, ScarlettPotentiometricNBS scale
46Temperature, waterTemp°CTrimborn, Scarlett
47SalinitySalTrimborn, Scarlett
48Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
49pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
50Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
51Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
52Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
53Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
54Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
55Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
56Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
57Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Size:
36257 data points

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