Rokitta, Sebastian D; John, Uwe; Rost, Björn (2012): Ocean Acidification Affects Redox-Balance and Ion-Homeostasis in the Life-Cycle Stages of Emiliania huxleyi [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.833669, Supplement to: Rokitta, SD et al. (2012): Ocean Acidification Affects Redox-Balance and Ion-Homeostasis in the Life-Cycle Stages of Emiliania huxleyi. PLoS ONE, 7(12), e52212, https://doi.org/10.1371/journal.pone.0052212
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Abstract:
Ocean Acidification (OA) has been shown to affect photosynthesis and calcification in the coccolithophore Emiliania huxleyi, a cosmopolitan calcifier that significantly contributes to the regulation of the biological carbon pumps. Its non-calcifying, haploid life-cycle stage was found to be relatively unaffected by OA with respect to biomass production. Deeper insights into physiological key processes and their dependence on environmental factors are lacking, but are required to understand and possibly estimate the dynamics of carbon cycling in present and future oceans. Therefore, calcifying diploid and non-calcifying haploid cells were acclimated to present and future CO2 partial pressures (pCO2; 38.5 Pa vs. 101.3 Pa CO2) under low and high light (50 vs. 300 µmol photons/m**2 /s). Comparative microarray-based transcriptome profiling was used to screen for the underlying cellular processes and allowed to follow up interpretations derived from physiological data. In the diplont, the observed increases in biomass production under OA are likely caused by stimulated production of glycoconjugates and lipids. The observed lowered calcification under OA can be attributed to impaired signal-transduction and ion-transport. The haplont utilizes distinct genes and metabolic pathways, reflecting the stage-specific usage of certain portions of the genome. With respect to functionality and energy-dependence, however, the transcriptomic OA-responses resemble those of the diplont. In both life-cycle stages, OA affects the cellular redox-state as a master regulator and thereby causes a metabolic shift from oxidative towards reductive pathways, which involves a reconstellation of carbon flux networks within and across compartments. Whereas signal transduction and ion-homeostasis appear equally OA-sensitive under both light intensities, the effects on carbon metabolism and light physiology are clearly modulated by light availability. These interactive effects can be attributed to the influence of OA and light on the redox equilibria of NAD and NADP, which function as major sensors for energization and stress. This generic mode of action of OA may therefore provoke similar cell-physiological responses in other protists.
Keyword(s):
Further details:
Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb
Project(s):
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
696 data points
Data
1 Species | 2 Strain | 3 Cat | 4 Group | 5 GA [#] (up-regulation) | 6 GA [#] (down-regulation) | 7 E [µmol/m2/s] | 8 pCO2water_SST_wet [Pa] | 9 pCO2 std dev [±] | 10 Sal | 11 Temp [°C] | 12 DIC [µmol/kg] | 13 DIC std dev [±] | 14 AT [µmol/kg] (Potentiometric titration) | 15 AT std dev [±] (Potentiometric titration) | 16 pH (NBS scale, Potentiometric) | 17 pH std dev [±] (NBS scale, Potentiometric) | 18 [HCO3]- [µmol/kg] (Calculated using CO2SYS) | 19 [HCO3]- std dev [±] (Calculated using CO2SYS) | 20 [CO3]2- [µmol/kg] (Calculated using CO2SYS) | 21 [CO3]2- std dev [±] (Calculated using CO2SYS) | 22 Omega Cal (Calculated using CO2SYS) | 23 Omega Cal std dev [±] (Calculated using CO2SYS) | 24 CSC flag (Calculated using seacarb afte...) | 25 pH (Total scale; Calculated from ...) | 26 CO2 [µmol/kg] (Calculated from means, Calcul...) | 27 pCO2water_SST_wet [µatm] (Calculated from means, Calcul...) | 28 fCO2water_SST_wet [µatm] (Calculated from means, Calcul...) | 29 [HCO3]- [µmol/kg] (Calculated from means, Calcul...) | 30 [CO3]2- [µmol/kg] (Calculated from means, Calcul...) | 31 DIC [µmol/kg] (Calculated from means, Calcul...) | 32 Omega Arg (Calculated from means, Calcul...) | 33 Omega Cal (Calculated from means, Calcul...) |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Emiliania huxleyi (phytoplankton) | RCC 1216 2N | Carbon metabolism | CO2 CORE RESPONSE | 47 | -29 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1216 2N | Light reactions | CO2 CORE RESPONSE | 11 | -3 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1216 2N | Signalling | CO2 CORE RESPONSE | 29 | -3 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1216 2N | Ion fluxes | CO2 CORE RESPONSE | 28 | -8 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1216 2N | Carbon metabolism | CO2 RESPONSE (Exclusive LL; A\C) | 21 | -11 | 50 | 97.3 | 11.1 | 32 | 15 | 2232 | 22 | 2385 | 7 | 7.808 | 0.012 | 2186 | 1 | 80.0 | 2.8 | 1.94 | 0.07 | 26 | 7.69 | 40 | 1055.69 | 1051.93 | 2202 | 75 | 2317 | 1.16 | 1.82 |
Emiliania huxleyi (phytoplankton) | RCC 1216 2N | Light reactions | CO2 RESPONSE (Exclusive LL; A\C) | 29 | -1 | 50 | 97.3 | 11.1 | 32 | 15 | 2232 | 22 | 2385 | 7 | 7.808 | 0.012 | 2186 | 1 | 80.0 | 2.8 | 1.94 | 0.07 | 26 | 7.69 | 40 | 1055.69 | 1051.93 | 2202 | 75 | 2317 | 1.16 | 1.82 |
Emiliania huxleyi (phytoplankton) | RCC 1216 2N | Signalling | CO2 RESPONSE (Exclusive LL; A\C) | 7 | -5 | 50 | 97.3 | 11.1 | 32 | 15 | 2232 | 22 | 2385 | 7 | 7.808 | 0.012 | 2186 | 1 | 80.0 | 2.8 | 1.94 | 0.07 | 26 | 7.69 | 40 | 1055.69 | 1051.93 | 2202 | 75 | 2317 | 1.16 | 1.82 |
Emiliania huxleyi (phytoplankton) | RCC 1216 2N | Ion fluxes | CO2 RESPONSE (Exclusive LL; A\C) | 7 | -3 | 50 | 97.3 | 11.1 | 32 | 15 | 2232 | 22 | 2385 | 7 | 7.808 | 0.012 | 2186 | 1 | 80.0 | 2.8 | 1.94 | 0.07 | 26 | 7.69 | 40 | 1055.69 | 1051.93 | 2202 | 75 | 2317 | 1.16 | 1.82 |
Emiliania huxleyi (phytoplankton) | RCC 1216 2N | Carbon metabolism | CO2 RESPONSE (Exclusive HL; B\C) | 32 | -8 | 300 | 110.6 | 14.6 | 32 | 15 | 2174 | 32 | 2322 | 35 | 7.741 | 0.002 | 2149 | 36 | 68.8 | 1.9 | 1.67 | 0.05 | 26 | 7.62 | 46 | 1221.43 | 1217.08 | 2168 | 63 | 2277 | 0.97 | 1.52 |
Emiliania huxleyi (phytoplankton) | RCC 1216 2N | Light reactions | CO2 RESPONSE (Exclusive HL; B\C) | 2 | -3 | 300 | 110.6 | 14.6 | 32 | 15 | 2174 | 32 | 2322 | 35 | 7.741 | 0.002 | 2149 | 36 | 68.8 | 1.9 | 1.67 | 0.05 | 26 | 7.62 | 46 | 1221.43 | 1217.08 | 2168 | 63 | 2277 | 0.97 | 1.52 |
Emiliania huxleyi (phytoplankton) | RCC 1216 2N | Signalling | CO2 RESPONSE (Exclusive HL; B\C) | 9 | 0 | 300 | 110.6 | 14.6 | 32 | 15 | 2174 | 32 | 2322 | 35 | 7.741 | 0.002 | 2149 | 36 | 68.8 | 1.9 | 1.67 | 0.05 | 26 | 7.62 | 46 | 1221.43 | 1217.08 | 2168 | 63 | 2277 | 0.97 | 1.52 |
Emiliania huxleyi (phytoplankton) | RCC 1216 2N | Ion fluxes | CO2 RESPONSE (Exclusive HL; B\C) | 0 | -1 | 300 | 110.6 | 14.6 | 32 | 15 | 2174 | 32 | 2322 | 35 | 7.741 | 0.002 | 2149 | 36 | 68.8 | 1.9 | 1.67 | 0.05 | 26 | 7.62 | 46 | 1221.43 | 1217.08 | 2168 | 63 | 2277 | 0.97 | 1.52 |
Emiliania huxleyi (phytoplankton) | RCC 1216 2N | Carbon metabolism | Light CORE RESPONSE (J) | 12 | -12 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1216 2N | Light reactions | Light CORE RESPONSE (J) | 8 | -1 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1216 2N | Signalling | Light CORE RESPONSE (J) | 1 | -1 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1216 2N | Ion fluxes | Light CORE RESPONSE (J) | 3 | -5 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1216 2N | Carbon metabolism | CO2 and Light Coregulation (O) | 5 | -2 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1216 2N | Light reactions | CO2 and Light Coregulation (O) | 4 | 0 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1216 2N | Signalling | CO2 and Light Coregulation (O) | 0 | 0 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1216 2N | Ion fluxes | CO2 and Light Coregulation (O) | 2 | -2 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1217 1N | Carbon metabolism | CO2 CORE RESPONSE | 39 | -17 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1217 1N | Light reactions | CO2 CORE RESPONSE | 2 | -9 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1217 1N | Signalling | CO2 CORE RESPONSE | 19 | -6 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1217 1N | Ion fluxes | CO2 CORE RESPONSE | 12 | -8 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1217 1N | Carbon metabolism | CO2 RESPONSE (Exclusive LL; A\C) | 30 | -10 | 50 | 95.7 | 1.9 | 32 | 15 | 2249 | 23 | 2398 | 3 | 7.823 | 0.022 | 2190 | 4 | 83.5 | 1.5 | 2.03 | 0.04 | 26 | 7.70 | 39 | 1035.51 | 1031.81 | 2211 | 77 | 2327 | 1.19 | 1.87 |
Emiliania huxleyi (phytoplankton) | RCC 1217 1N | Light reactions | CO2 RESPONSE (Exclusive LL; A\C) | 1 | -3 | 50 | 95.7 | 1.9 | 32 | 15 | 2249 | 23 | 2398 | 3 | 7.823 | 0.022 | 2190 | 4 | 83.5 | 1.5 | 2.03 | 0.04 | 26 | 7.70 | 39 | 1035.51 | 1031.81 | 2211 | 77 | 2327 | 1.19 | 1.87 |
Emiliania huxleyi (phytoplankton) | RCC 1217 1N | Signalling | CO2 RESPONSE (Exclusive LL; A\C) | 9 | -1 | 50 | 95.7 | 1.9 | 32 | 15 | 2249 | 23 | 2398 | 3 | 7.823 | 0.022 | 2190 | 4 | 83.5 | 1.5 | 2.03 | 0.04 | 26 | 7.70 | 39 | 1035.51 | 1031.81 | 2211 | 77 | 2327 | 1.19 | 1.87 |
Emiliania huxleyi (phytoplankton) | RCC 1217 1N | Ion fluxes | CO2 RESPONSE (Exclusive LL; A\C) | 6 | -2 | 50 | 95.7 | 1.9 | 32 | 15 | 2249 | 23 | 2398 | 3 | 7.823 | 0.022 | 2190 | 4 | 83.5 | 1.5 | 2.03 | 0.04 | 26 | 7.70 | 39 | 1035.51 | 1031.81 | 2211 | 77 | 2327 | 1.19 | 1.87 |
Emiliania huxleyi (phytoplankton) | RCC 1217 1N | Carbon metabolism | CO2 RESPONSE (Exclusive HL; B\C) | 39 | -33 | 300 | 95.2 | 5.9 | 32 | 15 | 2296 | 34 | 2402 | 21 | 7.818 | 0.038 | 2199 | 14 | 81.7 | 7.1 | 1.98 | 0.17 | 26 | 7.70 | 39 | 1036.98 | 1033.28 | 2214 | 77 | 2331 | 1.20 | 1.87 |
Emiliania huxleyi (phytoplankton) | RCC 1217 1N | Light reactions | CO2 RESPONSE (Exclusive HL; B\C) | 0 | -11 | 300 | 95.2 | 5.9 | 32 | 15 | 2296 | 34 | 2402 | 21 | 7.818 | 0.038 | 2199 | 14 | 81.7 | 7.1 | 1.98 | 0.17 | 26 | 7.70 | 39 | 1036.98 | 1033.28 | 2214 | 77 | 2331 | 1.20 | 1.87 |
Emiliania huxleyi (phytoplankton) | RCC 1217 1N | Signalling | CO2 RESPONSE (Exclusive HL; B\C) | 8 | -10 | 300 | 95.2 | 5.9 | 32 | 15 | 2296 | 34 | 2402 | 21 | 7.818 | 0.038 | 2199 | 14 | 81.7 | 7.1 | 1.98 | 0.17 | 26 | 7.70 | 39 | 1036.98 | 1033.28 | 2214 | 77 | 2331 | 1.20 | 1.87 |
Emiliania huxleyi (phytoplankton) | RCC 1217 1N | Ion fluxes | CO2 RESPONSE (Exclusive HL; B\C) | 5 | -5 | 300 | 95.2 | 5.9 | 32 | 15 | 2296 | 34 | 2402 | 21 | 7.818 | 0.038 | 2199 | 14 | 81.7 | 7.1 | 1.98 | 0.17 | 26 | 7.70 | 39 | 1036.98 | 1033.28 | 2214 | 77 | 2331 | 1.20 | 1.87 |
Emiliania huxleyi (phytoplankton) | RCC 1217 1N | Carbon metabolism | Light CORE RESPONSE (J) | 0 | -5 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1217 1N | Light reactions | Light CORE RESPONSE (J) | 1 | -1 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1217 1N | Signalling | Light CORE RESPONSE (J) | 2 | -2 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1217 1N | Ion fluxes | Light CORE RESPONSE (J) | 0 | -3 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1217 1N | Carbon metabolism | CO2 and Light Coregulation (O) | 0 | 0 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1217 1N | Light reactions | CO2 and Light Coregulation (O) | 0 | 0 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1217 1N | Signalling | CO2 and Light Coregulation (O) | 0 | 0 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1217 1N | Ion fluxes | CO2 and Light Coregulation (O) | 0 | 0 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1217 1N and RCC 1216 2N | Carbon metabolism | Synopsis: 1N and 2N common CO2 response (G) | 7 | -4 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1217 1N and RCC 1216 2N | Light reactions | Synopsis: 1N and 2N common CO2 response (G) | 0 | 0 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1217 1N and RCC 1216 2N | Signalling | Synopsis: 1N and 2N common CO2 response (G) | 10 | -1 | |||||||||||||||||||||||||||
Emiliania huxleyi (phytoplankton) | RCC 1217 1N and RCC 1216 2N | Ion fluxes | Synopsis: 1N and 2N common CO2 response (G) | 2 | -2 |