Citation:

j.jembe.2012.05.010

Name: Seawater acidification by CO2 in a coastal lagoon environment: Effects on life history traits of juvenile mussels Mytilus galloprovincialis
Published: 2012
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Growth/Morphology; Laboratory experiment; Mollusca; Mytilus galloprovincialis; North Atlantic; Single species; Temperate

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Abstract:

The carbonate chemistry of seawater from the Ria Formosa lagoon was experimentally manipulated, by diffusing pure CO2, to attain two reduced pH levels, by -0.3 and -0.6 pH units, relative to unmanipulated seawater. After 84 days of exposure, no differences were detected in terms of growth (somatic or shell) or mortality of juvenile mussels Mytilus galloprovincialis. The naturally elevated total alkalinity of the seawater (= 3550 µmol/kg) prevented under-saturation of CaCO3, even under pCO2 values exceeding 4000 µatm, attenuating the detrimental effects on the carbonate supply-side. Even so, variations in shell weight showed that net calcification was reduced under elevated CO2 and reduced pH, although the magnitude and significance of this effect varied among size-classes. Most of the loss of shell material probably occurred as post-deposition dissolution in the internal aragonitic nacre layer. Our results show that, even when reared under extreme levels of CO2-induced acidification, juvenile M. galloprovincialis can continue to calcify and grow in this coastal lagoon environment. The complex responses of bivalves to ocean acidification suggest a large degree of interspecific and intraspecific variability in their sensitivity to this type of perturbation. Further research is needed to assess the generality of these patterns and to disentangle the relative contributions of acclimation to local variations in seawater chemistry and genetic adaptation.

Keyword(s):

Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Growth/Morphology; Laboratory experiment; Mollusca; Mytilus galloprovincialis; North Atlantic; Single species; Temperate

Further details:

Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 43.389720 * Longitude: -8.290910

Date/Time Start: 2009-12-29T00:00:00 * Date/Time End: 2010-03-15T00:00:00

Event(s):

Ria_de_Ares_Betanzos * Latitude: 43.389720 * Longitude: -8.290910 * Date/Time Start: 2009-12-29T00:00:00 * Date/Time End: 2010-03-15T00:00:00 * Method/Device: Experiment (EXP) * Comment: Juvenile individuals of M. galloprovincialis were obtained from a mussel raft at the Ria de Ares-Betanzos

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-06-26.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Range, P
2	Table	Tab		Range, P
3	Figure	Fig		Range, P
4	Treatment	Treat		Range, P
5	Incubation duration	Inc dur	days	Range, P
6	Growth rate	µ	mm/day	Range, P
7	Size	Size		Range, P		µm
8	Mass	Mass	mg	Range, P		ashed shell weight
9	Mass, standard error	Mass std e	±	Range, P		ashed shell weight
10	Temperature, water	Temp	°C	Range, P
11	Temperature, water, standard error	T std e	±	Range, P
12	Salinity	Sal		Range, P
13	Salinity, standard error	Sal std e	±	Range, P
14	pH	pH		Range, P	Potentiometric	total scale
15	pH, standard error	pH std e	±	Range, P	Potentiometric	total scale
16	Alkalinity, total	AT	µmol/kg	Range, P	Potentiometric titration
17	Alkalinity, total, standard error	AT std e	±	Range, P	Potentiometric titration
18	Carbon, inorganic, dissolved	DIC	µmol/kg	Range, P	Calculated
19	Carbon, inorganic, dissolved, standard error	DIC std e	±	Range, P	Calculated
20	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Range, P	Calculated
21	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error	pCO2water_SST_wet std e	±	Range, P	Calculated
22	Calcite saturation state	Omega Cal		Range, P	Calculated
23	Calcite saturation state, standard error	Omega Cal std e	±	Range, P	Calculated
24	Aragonite saturation state	Omega Arg		Range, P	Calculated
25	Aragonite saturation state, standard error	Omega Arg std e	±	Range, P	Calculated
26	Phosphate	[PO4]3-	µmol/l	Range, P	Spectrophotometric
27	Phosphate, standard error	PO4 std e	±	Range, P	Spectrophotometric
28	Silicate	Si(OH)4	µmol/l	Range, P	Spectrophotometric
29	Silicate, standard error	Si(OH)4 std e	±	Range, P	Spectrophotometric
30	Mass	Mass	mg	Range, P		dry flesh weight
31	Mass, standard error	Mass std e	±	Range, P		dry flesh weight
32	Condition index	CI		Range, P
33	Condition index, standard error	CI std e	±	Range, P
34	Mass	Mass	mg	Range, P		ash-free shell weight
35	Mass, standard error	Mass std e	±	Range, P		ash-free shell weight
36	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

3090 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 Tab	3 Fig	4 Treat	5 Inc dur [days]	6 µ [mm/day]	7 Size (µm)	8 Mass [mg] (ashed shell weight)	9 Mass std e [±] (ashed shell weight)	10 Temp [°C]	11 T std e [±]	12 Sal	13 Sal std e [±]	14 pH (total scale, Potentiometric)	15 pH std e [±] (total scale, Potentiometric)	16 AT [µmol/kg] (Potentiometric titration)	17 AT std e [±] (Potentiometric titration)	18 DIC [µmol/kg] (Calculated)	19 DIC std e [±] (Calculated)	20 pCO2water_SST_wet [µatm] (Calculated)	21 pCO2water_SST_wet std e [±] (Calculated)	22 Omega Cal (Calculated)	23 Omega Cal std e [±] (Calculated)	24 Omega Arg (Calculated)	25 Omega Arg std e [±] (Calculated)	26 [PO4]3- [µmol/l] (Spectrophotometric)	27 PO4 std e [±] (Spectrophotometric)	28 Si(OH)4 [µmol/l] (Spectrophotometric)	29 Si(OH)4 std e [±] (Spectrophotometric)	30 Mass [mg] (dry flesh weight)	31 Mass std e [±] (dry flesh weight)	32 CI	33 CI std e [±]	34 Mass [mg] (ash-free shell weight)	35 Mass std e [±] (ash-free shell weight)	36 CSC flag (Calculated using seacarb afte...)	37 CO2 [µmol/kg] (Calculated using seacarb afte...)	38 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	39 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	40 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	41 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	42 DIC [µmol/kg] (Calculated using seacarb afte...)	43 Omega Arg (Calculated using seacarb afte...)	44 Omega Cal (Calculated using seacarb afte...)
Mytilus galloprovincialis (mollusk)		2	pH-CT	20	0.0534				17.73	0.02	28.79	0.01	7.88	0.01	3549	2	3303	3	963	14	5.53	0.07	3.58	0.05											8	36	1017	1014	3164	170	3370	2.69	4.23
Mytilus galloprovincialis (mollusk)		2	pH-CT	20	0.0408				17.73	0.02	28.79	0.01	7.88	0.01	3549	2	3303	3	963	14	5.53	0.07	3.58	0.05											8	36	1017	1014	3164	170	3370	2.69	4.23
Mytilus galloprovincialis (mollusk)		2	pH-CT	20	0.0524				17.73	0.02	28.79	0.01	7.88	0.01	3549	2	3303	3	963	14	5.53	0.07	3.58	0.05											8	36	1017	1014	3164	170	3370	2.69	4.23
Mytilus galloprovincialis (mollusk)		2	pH-0.3	20	0.0251				17.73	0.02	28.79	0.01	7.59	0.00	3542	2	3452	2	1989	19	3.03	0.00	1.96	0.00											8	74	2090	2082	3334	92	3500	1.45	2.28
Mytilus galloprovincialis (mollusk)		2	pH-0.3	20	0.0448				17.73	0.02	28.79	0.01	7.59	0.00	3542	2	3452	2	1989	19	3.03	0.00	1.96	0.00											8	74	2090	2082	3334	92	3500	1.45	2.28
Mytilus galloprovincialis (mollusk)		2	pH-0.3	20	0.0462				17.73	0.02	28.79	0.01	7.59	0.00	3542	2	3452	2	1989	19	3.03	0.00	1.96	0.00											8	74	2090	2082	3334	92	3500	1.45	2.28
Mytilus galloprovincialis (mollusk)		2	pH-0.6	20	0.0643				17.73	0.02	28.79	0.01	7.33	0.01	3554	16	3584	18	3790	55	1.72	0.03	1.11	0.02											8	140	3919	3906	3436	52	3628	0.82	1.29
Mytilus galloprovincialis (mollusk)		2	pH-0.6	20	0.0720				17.73	0.02	28.79	0.01	7.33	0.01	3554	16	3584	18	3790	55	1.72	0.03	1.11	0.02											8	140	3919	3906	3436	52	3628	0.82	1.29
Mytilus galloprovincialis (mollusk)		2	pH-0.6	20	0.0478				17.73	0.02	28.79	0.01	7.33	0.01	3554	16	3584	18	3790	55	1.72	0.03	1.11	0.02											8	140	3919	3906	3436	52	3628	0.82	1.29
Mytilus galloprovincialis (mollusk)		2	pH-CT	38	0.0492				17.73	0.02	28.79	0.01	7.88	0.01	3549	2	3303	3	963	14	5.53	0.07	3.58	0.05	3.73		91.70								8	36	1015	1012	3159	170	3364	2.68	4.22
Mytilus galloprovincialis (mollusk)		2	pH-CT	38	0.0374				17.73	0.02	28.79	0.01	7.88	0.01	3549	2	3303	3	963	14	5.53	0.07	3.58	0.05	3.73		91.70								8	36	1015	1012	3159	170	3364	2.68	4.22
Mytilus galloprovincialis (mollusk)		2	pH-CT	38	0.0401				17.73	0.02	28.79	0.01	7.88	0.01	3549	2	3303	3	963	14	5.53	0.07	3.58	0.05	3.73		91.70								8	36	1015	1012	3159	170	3364	2.68	4.22
Mytilus galloprovincialis (mollusk)		2	pH-0.3	38	0.0386				17.73	0.02	28.79	0.01	7.59	0.00	3542	2	3452	2	1989	19	3.03	0.00	1.96	0.00	3.73		91.70								8	74	2087	2080	3329	92	3495	1.45	2.28
Mytilus galloprovincialis (mollusk)		2	pH-0.3	38	0.0487				17.73	0.02	28.79	0.01	7.59	0.00	3542	2	3452	2	1989	19	3.03	0.00	1.96	0.00	3.73		91.70								8	74	2087	2080	3329	92	3495	1.45	2.28
Mytilus galloprovincialis (mollusk)		2	pH-0.3	38	0.0471				17.73	0.02	28.79	0.01	7.59	0.00	3542	2	3452	2	1989	19	3.03	0.00	1.96	0.00	3.73		91.70								8	74	2087	2080	3329	92	3495	1.45	2.28
Mytilus galloprovincialis (mollusk)		2	pH-0.6	38	0.0670				17.73	0.02	28.79	0.01	7.33	0.01	3554	16	3584	18	3790	55	1.72	0.03	1.11	0.02	3.73		91.70								8	139	3915	3901	3432	52	3624	0.82	1.29
Mytilus galloprovincialis (mollusk)		2	pH-0.6	38	0.0609				17.73	0.02	28.79	0.01	7.33	0.01	3554	16	3584	18	3790	55	1.72	0.03	1.11	0.02	3.73		91.70								8	139	3915	3901	3432	52	3624	0.82	1.29
Mytilus galloprovincialis (mollusk)		2	pH-0.6	38	0.0442				17.73	0.02	28.79	0.01	7.33	0.01	3554	16	3584	18	3790	55	1.72	0.03	1.11	0.02	3.73		91.70								8	139	3915	3901	3432	52	3624	0.82	1.29
Mytilus galloprovincialis (mollusk)		2	pH-CT	61	0.0557				16.50	0.02	28.87	0.02	7.88	0.01	3543	9	3289	10	977	15	5.73	0.18	3.72	0.13											8	37	1011	1008	3173	163	3373	2.57	4.04
Mytilus galloprovincialis (mollusk)		2	pH-CT	61	0.0391				16.50	0.02	28.87	0.02	7.88	0.01	3543	9	3289	10	977	15	5.73	0.18	3.72	0.13											8	37	1011	1008	3173	163	3373	2.57	4.04
Mytilus galloprovincialis (mollusk)		2	pH-CT	61	0.0476				16.50	0.02	28.87	0.02	7.88	0.01	3543	9	3289	10	977	15	5.73	0.18	3.72	0.13											8	37	1011	1008	3173	163	3373	2.57	4.04
Mytilus galloprovincialis (mollusk)		2	pH-0.3	61	0.0446				16.50	0.02	28.87	0.02	7.59	0.00	3555	4	3466	3	2051	14	3.03	0.03	1.96	0.02											8	77	2084	2077	3354	88	3520	1.39	2.19
Mytilus galloprovincialis (mollusk)		2	pH-0.3	61	0.0490				16.50	0.02	28.87	0.02	7.59	0.00	3555	4	3466	3	2051	14	3.03	0.03	1.96	0.02											8	77	2084	2077	3354	88	3520	1.39	2.19
Mytilus galloprovincialis (mollusk)		2	pH-0.3	61	0.0499				16.50	0.02	28.87	0.02	7.59	0.00	3555	4	3466	3	2051	14	3.03	0.03	1.96	0.02											8	77	2084	2077	3354	88	3520	1.39	2.19
Mytilus galloprovincialis (mollusk)		2	pH-0.6	61	0.0632				16.50	0.02	28.87	0.02	7.30	0.02	3544	8	3588	15	4193	184	1.61	0.06	1.05	0.04											8	154	4166	4151	3439	46	3639	0.73	1.15
Mytilus galloprovincialis (mollusk)		2	pH-0.6	61	0.0589				16.50	0.02	28.87	0.02	7.30	0.02	3544	8	3588	15	4193	184	1.61	0.06	1.05	0.04											8	154	4166	4151	3439	46	3639	0.73	1.15
Mytilus galloprovincialis (mollusk)		2	pH-0.6	61	0.0520				16.50	0.02	28.87	0.02	7.30	0.02	3544	8	3588	15	4193	184	1.61	0.06	1.05	0.04											8	154	4166	4151	3439	46	3639	0.73	1.15
Mytilus galloprovincialis (mollusk)		2	pH-CT	84	0.0497				16.50	0.02	28.87	0.02	7.88	0.01	3543	9	3289	10	977	15	5.73	0.18	3.72	0.13	2.51	0.25	78.64	1.28							8	37	1010	1006	3169	163	3369	2.56	4.04
Mytilus galloprovincialis (mollusk)		2	pH-CT	84	0.0393				16.50	0.02	28.87	0.02	7.88	0.01	3543	9	3289	10	977	15	5.73	0.18	3.72	0.13	2.51	0.25	78.64	1.28							8	37	1010	1006	3169	163	3369	2.56	4.04
Mytilus galloprovincialis (mollusk)		2	pH-CT	84	0.0417				16.50	0.02	28.87	0.02	7.88	0.01	3543	9	3289	10	977	15	5.73	0.18	3.72	0.13	2.51	0.25	78.64	1.28							8	37	1010	1006	3169	163	3369	2.56	4.04
Mytilus galloprovincialis (mollusk)		2	pH-0.3	84	0.0436				16.50	0.02	28.87	0.02	7.59	0.00	3555	4	3466	3	2051	14	3.03	0.03	1.96	0.02	2.51	0.25	78.64	1.28							8	77	2082	2075	3351	88	3516	1.39	2.19
Mytilus galloprovincialis (mollusk)		2	pH-0.3	84	0.0460				16.50	0.02	28.87	0.02	7.59	0.00	3555	4	3466	3	2051	14	3.03	0.03	1.96	0.02	2.51	0.25	78.64	1.28							8	77	2082	2075	3351	88	3516	1.39	2.19
Mytilus galloprovincialis (mollusk)		2	pH-0.3	84	0.0498				16.50	0.02	28.87	0.02	7.59	0.00	3555	4	3466	3	2051	14	3.03	0.03	1.96	0.02	2.51	0.25	78.64	1.28							8	77	2082	2075	3351	88	3516	1.39	2.19
Mytilus galloprovincialis (mollusk)		2	pH-0.6	84	0.0546				16.50	0.02	28.87	0.02	7.30	0.02	3544	8	3588	15	4193	184	1.61	0.06	1.05	0.04	2.51	0.25	78.64	1.28							8	154	4162	4148	3436	46	3636	0.73	1.15
Mytilus galloprovincialis (mollusk)		2	pH-0.6	84	0.0473				16.50	0.02	28.87	0.02	7.30	0.02	3544	8	3588	15	4193	184	1.61	0.06	1.05	0.04	2.51	0.25	78.64	1.28							8	154	4162	4148	3436	46	3636	0.73	1.15
Mytilus galloprovincialis (mollusk)		2	pH-0.6	84	0.0409				16.50	0.02	28.87	0.02	7.30	0.02	3544	8	3588	15	4193	184	1.61	0.06	1.05	0.04	2.51	0.25	78.64	1.28							8	154	4162	4148	3436	46	3636	0.73	1.15
Mytilus galloprovincialis (mollusk)		3	pH-CT			14-16	88.90		16.50	0.02	28.87	0.02	7.88	0.01	3543	9	3289	10	977	15	5.73	0.18	3.72	0.13	2.51	0.25	78.64	1.28							8	37	1010	1006	3169	163	3369	2.56	4.04
Mytilus galloprovincialis (mollusk)		3	pH-CT			14-16	102.50		16.50	0.02	28.87	0.02	7.88	0.01	3543	9	3289	10	977	15	5.73	0.18	3.72	0.13	2.51	0.25	78.64	1.28							8	37	1010	1006	3169	163	3369	2.56	4.04
Mytilus galloprovincialis (mollusk)		3	pH-CT			14-16	98.40		16.50	0.02	28.87	0.02	7.88	0.01	3543	9	3289	10	977	15	5.73	0.18	3.72	0.13	2.51	0.25	78.64	1.28							8	37	1010	1006	3169	163	3369	2.56	4.04
Mytilus galloprovincialis (mollusk)		3	pH-0.3			14-16	113.40		16.50	0.02	28.87	0.02	7.59	0.00	3555	4	3466	3	2051	14	3.03	0.03	1.96	0.02	2.51	0.25	78.64	1.28							8	77	2082	2075	3351	88	3516	1.39	2.19
Mytilus galloprovincialis (mollusk)		3	pH-0.3			14-16	99.00		16.50	0.02	28.87	0.02	7.59	0.00	3555	4	3466	3	2051	14	3.03	0.03	1.96	0.02	2.51	0.25	78.64	1.28							8	77	2082	2075	3351	88	3516	1.39	2.19
Mytilus galloprovincialis (mollusk)		3	pH-0.3			14-16	91.30		16.50	0.02	28.87	0.02	7.59	0.00	3555	4	3466	3	2051	14	3.03	0.03	1.96	0.02	2.51	0.25	78.64	1.28							8	77	2082	2075	3351	88	3516	1.39	2.19
Mytilus galloprovincialis (mollusk)		3	pH-0.6			14-16	98.70		16.50	0.02	28.87	0.02	7.30	0.02	3544	8	3588	15	4193	184	1.61	0.06	1.05	0.04	2.51	0.25	78.64	1.28							8	154	4162	4148	3436	46	3636	0.73	1.15
Mytilus galloprovincialis (mollusk)		3	pH-0.6			14-16	100.90		16.50	0.02	28.87	0.02	7.30	0.02	3544	8	3588	15	4193	184	1.61	0.06	1.05	0.04	2.51	0.25	78.64	1.28							8	154	4162	4148	3436	46	3636	0.73	1.15
Mytilus galloprovincialis (mollusk)		3	pH-0.6			14-16	96.10		16.50	0.02	28.87	0.02	7.30	0.02	3544	8	3588	15	4193	184	1.61	0.06	1.05	0.04	2.51	0.25	78.64	1.28							8	154	4162	4148	3436	46	3636	0.73	1.15
Mytilus galloprovincialis (mollusk)		3	pH-CT			16-18	128.50		16.50	0.02	28.87	0.02	7.88	0.01	3543	9	3289	10	977	15	5.73	0.18	3.72	0.13	2.51	0.25	78.64	1.28							8	37	1010	1006	3169	163	3369	2.56	4.04
Mytilus galloprovincialis (mollusk)		3	pH-CT			16-18	125.70		16.50	0.02	28.87	0.02	7.88	0.01	3543	9	3289	10	977	15	5.73	0.18	3.72	0.13	2.51	0.25	78.64	1.28							8	37	1010	1006	3169	163	3369	2.56	4.04
Mytilus galloprovincialis (mollusk)		3	pH-CT			16-18	126.40		16.50	0.02	28.87	0.02	7.88	0.01	3543	9	3289	10	977	15	5.73	0.18	3.72	0.13	2.51	0.25	78.64	1.28							8	37	1010	1006	3169	163	3369	2.56	4.04
Mytilus galloprovincialis (mollusk)		3	pH-0.3			16-18	133.70		16.50	0.02	28.87	0.02	7.59	0.00	3555	4	3466	3	2051	14	3.03	0.03	1.96	0.02	2.51	0.25	78.64	1.28							8	77	2082	2075	3351	88	3516	1.39	2.19
Mytilus galloprovincialis (mollusk)		3	pH-0.3			16-18	129.00		16.50	0.02	28.87	0.02	7.59	0.00	3555	4	3466	3	2051	14	3.03	0.03	1.96	0.02	2.51	0.25	78.64	1.28							8	77	2082	2075	3351	88	3516	1.39	2.19
Mytilus galloprovincialis (mollusk)		3	pH-0.3			16-18	133.00		16.50	0.02	28.87	0.02	7.59	0.00	3555	4	3466	3	2051	14	3.03	0.03	1.96	0.02	2.51	0.25	78.64	1.28							8	77	2082	2075	3351	88	3516	1.39	2.19
Mytilus galloprovincialis (mollusk)		3	pH-0.6			16-18	134.80		16.50	0.02	28.87	0.02	7.30	0.02	3544	8	3588	15	4193	184	1.61	0.06	1.05	0.04	2.51	0.25	78.64	1.28							8	154	4162	4148	3436	46	3636	0.73	1.15
Mytilus galloprovincialis (mollusk)		3	pH-0.6			16-18	134.40		16.50	0.02	28.87	0.02	7.30	0.02	3544	8	3588	15	4193	184	1.61	0.06	1.05	0.04	2.51	0.25	78.64	1.28							8	154	4162	4148	3436	46	3636	0.73	1.15
Mytilus galloprovincialis (mollusk)		3	pH-0.6			16-18	137.10		16.50	0.02	28.87	0.02	7.30	0.02	3544	8	3588	15	4193	184	1.61	0.06	1.05	0.04	2.51	0.25	78.64	1.28							8	154	4162	4148	3436	46	3636	0.73	1.15
Mytilus galloprovincialis (mollusk)		3	pH-CT			18-20	160.00		16.50	0.02	28.87	0.02	7.88	0.01	3543	9	3289	10	977	15	5.73	0.18	3.72	0.13	2.51	0.25	78.64	1.28							8	37	1010	1006	3169	163	3369	2.56	4.04
Mytilus galloprovincialis (mollusk)		3	pH-CT			18-20	166.50		16.50	0.02	28.87	0.02	7.88	0.01	3543	9	3289	10	977	15	5.73	0.18	3.72	0.13	2.51	0.25	78.64	1.28							8	37	1010	1006	3169	163	3369	2.56	4.04
Mytilus galloprovincialis (mollusk)		3	pH-CT			18-20	177.70		16.50	0.02	28.87	0.02	7.88	0.01	3543	9	3289	10	977	15	5.73	0.18	3.72	0.13	2.51	0.25	78.64	1.28							8	37	1010	1006	3169	163	3369	2.56	4.04
Mytilus galloprovincialis (mollusk)		3	pH-0.3			18-20	171.90		16.50	0.02	28.87	0.02	7.59	0.00	3555	4	3466	3	2051	14	3.03	0.03	1.96	0.02	2.51	0.25	78.64	1.28							8	77	2082	2075	3351	88	3516	1.39	2.19
Mytilus galloprovincialis (mollusk)		3	pH-0.3			18-20	174.30		16.50	0.02	28.87	0.02	7.59	0.00	3555	4	3466	3	2051	14	3.03	0.03	1.96	0.02	2.51	0.25	78.64	1.28							8	77	2082	2075	3351	88	3516	1.39	2.19
Mytilus galloprovincialis (mollusk)		3	pH-0.3			18-20	173.50		16.50	0.02	28.87	0.02	7.59	0.00	3555	4	3466	3	2051	14	3.03	0.03	1.96	0.02	2.51	0.25	78.64	1.28							8	77	2082	2075	3351	88	3516	1.39	2.19
Mytilus galloprovincialis (mollusk)		3	pH-0.6			18-20	178.40		16.50	0.02	28.87	0.02	7.30	0.02	3544	8	3588	15	4193	184	1.61	0.06	1.05	0.04	2.51	0.25	78.64	1.28							8	154	4162	4148	3436	46	3636	0.73	1.15
Mytilus galloprovincialis (mollusk)		3	pH-0.6			18-20	163.10		16.50	0.02	28.87	0.02	7.30	0.02	3544	8	3588	15	4193	184	1.61	0.06	1.05	0.04	2.51	0.25	78.64	1.28							8	154	4162	4148	3436	46	3636	0.73	1.15
Mytilus galloprovincialis (mollusk)		3	pH-0.6			18-20	182.30		16.50	0.02	28.87	0.02	7.30	0.02	3544	8	3588	15	4193	184	1.61	0.06	1.05	0.04	2.51	0.25	78.64	1.28							8	154	4162	4148	3436	46	3636	0.73	1.15
Mytilus galloprovincialis (mollusk)		3	pH-CT			20-22	192.80		16.50	0.02	28.87	0.02	7.88	0.01	3543	9	3289	10	977	15	5.73	0.18	3.72	0.13	2.51	0.25	78.64	1.28							8	37	1010	1006	3169	163	3369	2.56	4.04
Mytilus galloprovincialis (mollusk)		3	pH-CT			20-22	218.10		16.50	0.02	28.87	0.02	7.88	0.01	3543	9	3289	10	977	15	5.73	0.18	3.72	0.13	2.51	0.25	78.64	1.28							8	37	1010	1006	3169	163	3369	2.56	4.04
Mytilus galloprovincialis (mollusk)		3	pH-CT			20-22	217.30		16.50	0.02	28.87	0.02	7.88	0.01	3543	9	3289	10	977	15	5.73	0.18	3.72	0.13	2.51	0.25	78.64	1.28							8	37	1010	1006	3169	163	3369	2.56	4.04
Mytilus galloprovincialis (mollusk)		3	pH-0.3			20-22	226.10		16.50	0.02	28.87	0.02	7.59	0.00	3555	4	3466	3	2051	14	3.03	0.03	1.96	0.02	2.51	0.25	78.64	1.28							8	77	2082	2075	3351	88	3516	1.39	2.19
Mytilus galloprovincialis (mollusk)		3	pH-0.3			20-22	224.40		16.50	0.02	28.87	0.02	7.59	0.00	3555	4	3466	3	2051	14	3.03	0.03	1.96	0.02	2.51	0.25	78.64	1.28							8	77	2082	2075	3351	88	3516	1.39	2.19
Mytilus galloprovincialis (mollusk)		3	pH-0.3			20-22	221.20		16.50	0.02	28.87	0.02	7.59	0.00	3555	4	3466	3	2051	14	3.03	0.03	1.96	0.02	2.51	0.25	78.64	1.28							8	77	2082	2075	3351	88	3516	1.39	2.19
Mytilus galloprovincialis (mollusk)		3	pH-0.6			20-22	195.70		16.50	0.02	28.87	0.02	7.30	0.02	3544	8	3588	15	4193	184	1.61	0.06	1.05	0.04	2.51	0.25	78.64	1.28							8	154	4162	4148	3436	46	3636	0.73	1.15
Mytilus galloprovincialis (mollusk)		3	pH-0.6			20-22	226.50		16.50	0.02	28.87	0.02	7.30	0.02	3544	8	3588	15	4193	184	1.61	0.06	1.05	0.04	2.51	0.25	78.64	1.28							8	154	4162	4148	3436	46	3636	0.73	1.15
Mytilus galloprovincialis (mollusk)		3	pH-0.6			20-22	230.50		16.50	0.02	28.87	0.02	7.30	0.02	3544	8	3588	15	4193	184	1.61	0.06	1.05	0.04	2.51	0.25	78.64	1.28							8	154	4162	4148	3436	46	3636	0.73	1.15
Mytilus galloprovincialis (mollusk)		3	pH-CT			22-24	260.00		16.50	0.02	28.87	0.02	7.88	0.01	3543	9	3289	10	977	15	5.73	0.18	3.72	0.13	2.51	0.25	78.64	1.28							8	37	1010	1006	3169	163	3369	2.56	4.04
Mytilus galloprovincialis (mollusk)		3	pH-CT			22-24	277.40		16.50	0.02	28.87	0.02	7.88	0.01	3543	9	3289	10	977	15	5.73	0.18	3.72	0.13	2.51	0.25	78.64	1.28							8	37	1010	1006	3169	163	3369	2.56	4.04
Mytilus galloprovincialis (mollusk)		3	pH-CT			22-24	247.40		16.50	0.02	28.87	0.02	7.88	0.01	3543	9	3289	10	977	15	5.73	0.18	3.72	0.13	2.51	0.25	78.64	1.28							8	37	1010	1006	3169	163	3369	2.56	4.04
Mytilus galloprovincialis (mollusk)		3	pH-0.3			22-24	264.00		16.50	0.02	28.87	0.02	7.59	0.00	3555	4	3466	3	2051	14	3.03	0.03	1.96	0.02	2.51	0.25	78.64	1.28							8	77	2082	2075	3351	88	3516	1.39	2.19
Mytilus galloprovincialis (mollusk)		3	pH-0.3			22-24	276.20		16.50	0.02	28.87	0.02	7.59	0.00	3555	4	3466	3	2051	14	3.03	0.03	1.96	0.02	2.51	0.25	78.64	1.28							8	77	2082	2075	3351	88	3516	1.39	2.19
Mytilus galloprovincialis (mollusk)		3	pH-0.3			22-24	276.10		16.50	0.02	28.87	0.02	7.59	0.00	3555	4	3466	3	2051	14	3.03	0.03	1.96	0.02	2.51	0.25	78.64	1.28							8	77	2082	2075	3351	88	3516	1.39	2.19
Mytilus galloprovincialis (mollusk)		3	pH-0.6			22-24	286.80		16.50	0.02	28.87	0.02	7.30	0.02	3544	8	3588	15	4193	184	1.61	0.06	1.05	0.04	2.51	0.25	78.64	1.28							8	154	4162	4148	3436	46	3636	0.73	1.15
Mytilus galloprovincialis (mollusk)		3	pH-0.6			22-24	284.70		16.50	0.02	28.87	0.02	7.30	0.02	3544	8	3588	15	4193	184	1.61	0.06	1.05	0.04	2.51	0.25	78.64	1.28							8	154	4162	4148	3436	46	3636	0.73	1.15
Mytilus galloprovincialis (mollusk)		3	pH-0.6			22-24	281.50		16.50	0.02	28.87	0.02	7.30	0.02	3544	8	3588	15	4193	184	1.61	0.06	1.05	0.04	2.51	0.25	78.64	1.28							8	154	4162	4148	3436	46	3636	0.73	1.15
Mytilus galloprovincialis (mollusk)		3	pH-CT			24-26	313.80		16.50	0.02	28.87	0.02	7.88	0.01	3543	9	3289	10	977	15	5.73	0.18	3.72	0.13	2.51	0.25	78.64	1.28							8	37	1010	1006	3169	163	3369	2.56	4.04
Mytilus galloprovincialis (mollusk)		3	pH-CT			24-26	318.20		16.50	0.02	28.87	0.02	7.88	0.01	3543	9	3289	10	977	15	5.73	0.18	3.72	0.13	2.51	0.25	78.64	1.28							8	37	1010	1006	3169	163	3369	2.56	4.04
Mytilus galloprovincialis (mollusk)		3	pH-CT			24-26	311.80		16.50	0.02	28.87	0.02	7.88	0.01	3543	9	3289	10	977	15	5.73	0.18	3.72	0.13	2.51	0.25	78.64	1.28							8	37	1010	1006	3169	163	3369	2.56	4.04
Mytilus galloprovincialis (mollusk)		3	pH-0.3			24-26	328.00		16.50	0.02	28.87	0.02	7.59	0.00	3555	4	3466	3	2051	14	3.03	0.03	1.96	0.02	2.51	0.25	78.64	1.28							8	77	2082	2075	3351	88	3516	1.39	2.19
Mytilus galloprovincialis (mollusk)		3	pH-0.3			24-26	303.60		16.50	0.02	28.87	0.02	7.59	0.00	3555	4	3466	3	2051	14	3.03	0.03	1.96	0.02	2.51	0.25	78.64	1.28							8	77	2082	2075	3351	88	3516	1.39	2.19
Mytilus galloprovincialis (mollusk)		3	pH-0.3			24-26	319.10		16.50	0.02	28.87	0.02	7.59	0.00	3555	4	3466	3	2051	14	3.03	0.03	1.96	0.02	2.51	0.25	78.64	1.28							8	77	2082	2075	3351	88	3516	1.39	2.19
Mytilus galloprovincialis (mollusk)		3	pH-0.6			24-26	351.90		16.50	0.02	28.87	0.02	7.30	0.02	3544	8	3588	15	4193	184	1.61	0.06	1.05	0.04	2.51	0.25	78.64	1.28							8	154	4162	4148	3436	46	3636	0.73	1.15
Mytilus galloprovincialis (mollusk)		3	pH-0.6			24-26	334.80		16.50	0.02	28.87	0.02	7.30	0.02	3544	8	3588	15	4193	184	1.61	0.06	1.05	0.04	2.51	0.25	78.64	1.28							8	154	4162	4148	3436	46	3636	0.73	1.15
Mytilus galloprovincialis (mollusk)		3	pH-0.6			24-26	350.60		16.50	0.02	28.87	0.02	7.30	0.02	3544	8	3588	15	4193	184	1.61	0.06	1.05	0.04	2.51	0.25	78.64	1.28							8	154	4162	4148	3436	46	3636	0.73	1.15
Mytilus galloprovincialis (mollusk)	5		pH-CT				212.60	2.70	16.50	0.02	28.87	0.02	7.88	0.01	3543	9	3289	10	977	15	5.73	0.18	3.72	0.13	2.51	0.25	78.64	1.28	21.08	1.29	0.0917	0.0007	10.99	0.44	8	37	1010	1006	3169	163	3369	2.56	4.04
Mytilus galloprovincialis (mollusk)	5		pH-0.3				203.21	1.53	16.50	0.02	28.87	0.02	7.59	0.00	3555	4	3466	3	2051	14	3.03	0.03	1.96	0.02	2.51	0.25	78.64	1.28	19.10	0.79	0.0869	0.0040	9.80	0.35	8	77	2082	2075	3351	88	3516	1.39	2.19
Mytilus galloprovincialis (mollusk)	5		pH-0.6				199.26	1.45	16.50	0.02	28.87	0.02	7.30	0.02	3544	8	3588	15	4193	184	1.61	0.06	1.05	0.04	2.51	0.25	78.64	1.28	21.85	0.03	0.0993	0.0009	9.76	0.47	8	154	4162	4148	3436	46	3636	0.73	1.15