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Bögner, Desislava; Bickmeyer, Ulf; Köhler, Angela (2014): CO2-induced fertilization impairment in Strongylocentrotus droebachiensis collected in the Arctic [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.833621, Supplement to: Bögner, D et al. (2014): CO2-induced fertilization impairment in Strongylocentrotus droebachiensis collected in the Arctic. Helgoland Marine Research, 68(2), 341-356, https://doi.org/10.1007/s10152-014-0394-3

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Abstract:
Fertilization depends on distribution and aggregation patterns of sea urchins which influence gamete contact time and may potentially enhance their vulnerability to ocean acidification. In this study, we conducted fertilization experiments to assess the effects of selected pH scenarios on fertilization success of Strongylocentrotus droebachiensis, from Spitsbergen, Arctic. Acidification was achieved by aerating seawater with different CO2 partial pressures to represent pre-industrial and present conditions (measured ~180-425 µatm) and future acidification scenarios (~550-800, ~1,300, ~2,000 µatm). Fertilization success was defined as the proportion of successful/unsuccessful fertilizations per treatment; eggs were classified according to features of their fertilization envelope (FE), hyaline layer (HL) and achievement of cellular division. The diagnostic findings of specific pathological aberrations were described in detail. We additionally measured intracellular pH changes in unfertilized eggs exposed for 1 h to selected acidification treatments using BCECF/AM. We conclude that (a) acidified conditions increase the proportion of eggs that failed fertilization, (b) acidification may increase the risk of polyspermy due to failures in the FE formation supported by the occasional observation of multiple sperms in the perivitelline space and (c) irregular formation of the embryo may arise due to impaired formation of the HL. The decrease in fertilization success could be also related to the observed changes in intracellular pH at pCO2 ~ 1,000 µatm or higher.
Keyword(s):
Animalia; Arctic; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Echinodermata; Laboratory experiment; Polar; Reproduction; Single species; Strongylocentrotus droebachiensis
Further details:
Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-06-19.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1SpeciesSpeciesBickmeyer, Ulf
2ExperimentExpBickmeyer, Ulf
3ReplicateReplBickmeyer, Ulf
4Time in hoursTimehBickmeyer, Ulffertilization time
5TreatmentTreatBickmeyer, UlfpCO2 (µatm)
6StageStageBickmeyer, UlfMediterranean stagesfertilization
7CommentCommentBickmeyer, Ulf
8EggsEggs#Bickmeyer, Ulf
9Egg stagesEgg%Bickmeyer, Ulf
10Relative fluorescence intensity, ratioQBickmeyer, Ulfratio 490/439
11SalinitySalBickmeyer, Ulf
12Salinity, standard deviationSal std dev±Bickmeyer, Ulf
13Alkalinity, totalATµmol/kgBickmeyer, UlfPotentiometric titration
14Alkalinity, total, standard deviationAT std dev±Bickmeyer, UlfPotentiometric titration
15pHpHBickmeyer, UlfPotentiometricNBS scale
16pH, standard deviationpH std dev±Bickmeyer, UlfPotentiometricNBS scale
17Carbon, inorganic, dissolvedDICµmol/kgBickmeyer, UlfCoulometric titration
18Carbon, inorganic, dissolved, standard deviationDIC std dev±Bickmeyer, UlfCoulometric titration
19pHpHBickmeyer, UlfCalculated using CO2SYStotal scale
20pH, standard deviationpH std dev±Bickmeyer, UlfCalculated using CO2SYStotal scale
21Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmBickmeyer, UlfCalculated using CO2SYS
22Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Bickmeyer, UlfCalculated using CO2SYS
23Temperature, waterTemp°CBickmeyer, Ulf
24Temperature, water, standard deviationTemp std dev±Bickmeyer, Ulf
25Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
26pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
27Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
28Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
29Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
30Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
31Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
32Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
33Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
34Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
68836 data points

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