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Vihtakari, Mikko; Hendriks, Iris; Holding, Johnna; Renaud, Paul E; Duarte, Carlos M; Havenhand, Jon N (2013): Effects of Ocean Acidification and Warming on Sperm Activity and Early Life Stages of the Mediterranean Mussel (Mytilus galloprovincialis). doi:10.1594/PANGAEA.833257, Supplement to: Vihtakari, M et al. (2013): Effects of Ocean Acidification and Warming on Sperm Activity and Early Life Stages of the Mediterranean Mussel (Mytilus galloprovincialis). Water, 5(4), 1890-1915, doi:10.3390/w5041890

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Abstract:
Larval stages are among those most vulnerable to ocean acidification (OA). Projected atmospheric CO2 levels for the end of this century may lead to negative impacts on communities dominated by calcifying taxa with planktonic life stages. We exposed Mediterranean mussel (Mytilus galloprovincialis) sperm and early life stages to pHT levels of 8.0 (current pH) and 7.6 (2100 level) by manipulating pCO2 level (380 and 1000 ppm). Sperm activity was examined at ambient temperatures (16-17 °C) using individual males as replicates. We also assessed the effects of temperature (ambient and = 20 °C) and pH on larval size, survival, respiration and calcification of late trochophore/early D-veliger stages using a cross-factorial design. Increased pCO2 had a negative effect on the percentage of motile sperm (mean response ratio R= 71%) and sperm swimming speed (R= 74%), possibly indicating reduced fertilization capacity of sperm in low concentrations. Increased temperature had a more prominent effect on larval stages than pCO2, reducing performance (RSize = 90% and RSurvival = 70%) and increasing energy demand (RRespiration = 429%). We observed no significant interactions between pCO2 and temperature. Our results suggest that increasing temperature might have a larger impact on very early larval stages of M. galloprovincialis than OA at levels predicted for the end of the century.
Further details:
Lavigne, Héloise; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-06-05.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1SpeciesSpeciesVihtakari, Mikko
2Individual codeIndividual codeVihtakari, Mikko
3IdentificationIDVihtakari, Mikko
4TreatmentTreatVihtakari, Mikko
5SizeSizeµmVihtakari, Mikkomax
6LarvaeLarvae#Vihtakari, Mikkoaverage
7Respiration rate, oxygenResp O2µmol/l/hVihtakari, Mikko
8Respiration rate, oxygen, standard errorResp O2 std e±Vihtakari, Mikko
9Abundance per volumeAbund v#/m3Vihtakari, Mikko
10Respiration rate, oxygen, per individualResp O2/indpmol/#/hVihtakari, Mikko
11Respiration rate, oxygen, standard errorResp O2 std e±Vihtakari, Mikko
12DateDateVihtakari, Mikko
13Calcification rate of carbonCalc rate Cµg/l/dayVihtakari, Mikko
14Calcification rate of carbon per individualCalc rate C/indµg/#/dayVihtakari, Mikko
15ReplicateReplicateVihtakari, Mikko
16LarvaeLarvae#Vihtakari, Mikkoinitial, per cylinder
17LarvaeLarvae#Vihtakari, Mikko58 h after fertilization, per cylinder
18SurvivalSurvival%Vihtakari, Mikko
19Sperm motilitySperm motility%Vihtakari, Mikko
20Sperm motility, standard errorSperm motility std e±Vihtakari, Mikko
21Sperm motility, standard deviationSperm motility std dev±Vihtakari, Mikko
22ReplicatesRepl#Vihtakari, Mikko
23Speed, swimmingSp swimµm/sVihtakari, Mikkosperm
24Speed, swimming, standard errorSp swim std e±Vihtakari, Mikkosperm
25Speed, swimming, standard deviationSp swim std dev±Vihtakari, Mikkosperm
26SalinitySalVihtakari, Mikko
27Temperature, waterTemp°CVihtakari, Mikko0.5 h after fertilization
28Temperature, water, standard deviationTemp std dev±Vihtakari, Mikko0.5 h after fertilization
29pHpHVihtakari, MikkoSpectrophotometrictotal scale, measured at 25 °C, 0.5 h after fertilization
30pH, standard deviationpH std dev±Vihtakari, MikkoSpectrophotometrictotal scale, measured at 25 °C, 0.5 h after fertilization
31Carbon, inorganic, dissolvedDICµmol/kgVihtakari, Mikko0.5 h after fertilization
32Carbon, inorganic, dissolved, standard deviationDIC std dev±Vihtakari, Mikko0.5 h after fertilization
33pHpHVihtakari, MikkoCalculated using CO2SYStotal scale, in situ, 0.5 h after fertilization
34pH, standard deviationpH std dev±Vihtakari, MikkoCalculated using CO2SYStotal scale, in situ, 0.5 h after fertilization
35Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetppmvVihtakari, MikkoCalculated using CO2SYS0.5 h after fertilization
36Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Vihtakari, MikkoCalculated using CO2SYS0.5 h after fertilization
37Alkalinity, totalATµmol/kgVihtakari, MikkoCalculated using CO2SYS0.5 h after fertilization
38Alkalinity, total, standard deviationAT std dev±Vihtakari, MikkoCalculated using CO2SYS0.5 h after fertilization
39Aragonite saturation stateOmega ArgVihtakari, MikkoCalculated using CO2SYS0.5 h after fertilization
40Aragonite saturation state, standard deviationOmega Arg std dev±Vihtakari, MikkoCalculated using CO2SYS0.5 h after fertilization
41Temperature, waterTemp°CVihtakari, Mikko47.5 h after fertilization
42Temperature, water, standard deviationTemp std dev±Vihtakari, Mikko47.5 h after fertilization
43pHpHVihtakari, MikkoSpectrophotometrictotal scale, measured at 25 °C, 47.5 h after fertilization
44pH, standard deviationpH std dev±Vihtakari, MikkoSpectrophotometrictotal scale, measured at 25 °C, 47.5 h after fertilization
45Carbon, inorganic, dissolvedDICµmol/kgVihtakari, Mikko47.5 h after fertilization
46Carbon, inorganic, dissolved, standard deviationDIC std dev±Vihtakari, Mikko47.5 h after fertilization
47pHpHVihtakari, MikkoCalculated using CO2SYStotal scale, in situ, 47.5 h after fertilization
48pH, standard deviationpH std dev±Vihtakari, MikkoCalculated using CO2SYStotal scale, in situ, 47.5 h after fertilization
49Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetppmvVihtakari, MikkoCalculated using CO2SYS47.5 h after fertilization
50Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Vihtakari, MikkoCalculated using CO2SYS47.5 h after fertilization
51Alkalinity, totalATµmol/kgVihtakari, MikkoCalculated using CO2SYS47.5 h after fertilization
52Alkalinity, total, standard deviationAT std dev±Vihtakari, MikkoCalculated using CO2SYS47.5 h after fertilization
53Aragonite saturation stateOmega ArgVihtakari, MikkoCalculated using CO2SYS47.5 h after fertilization
54Aragonite saturation state, standard deviationOmega Arg std dev±Vihtakari, MikkoCalculated using CO2SYS47.5 h after fertilization
55Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
56pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale, 0.5 h after fertilization
57Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)0.5 h after fertilization
58Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)0.5 h after fertilization
59Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)0.5 h after fertilization
60Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)0.5 h after fertilization
61Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)0.5 h after fertilization
62Alkalinity, totalATµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)0.5 h after fertilization
63Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)0.5 h after fertilization
64Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)0.5 h after fertilization
65pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale, 0.5 h after fertilization
66Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)47.5 h after fertilization
67Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)47.5 h after fertilization
68Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)47.5 h after fertilization
69Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)47.5 h after fertilization
70Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)47.5 h after fertilization
71Alkalinity, totalATµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)47.5 h after fertilization
72Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)47.5 h after fertilization
73Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)47.5 h after fertilization
Size:
36718 data points

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