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Stumpp, Meike; Hu, Marian Y; Melzner, Frank; Gutowska, Magdalena A; Dorey, Narimane; Himmerkus, Nina; Holtmann, Wiebke C; Dupont, Sam; Thorndyke, Mike; Bleich, Markus (2012): Experiment: Acidified seawater impacts sea urchin larvae pH regulatory systems relevant for calcification. doi:10.1594/PANGAEA.833111,
Supplement to: Stumpp, M et al. (2012): Acidified seawater impacts sea urchin larvae pH regulatory systems relevant for calcification. Proceedings of the National Academy of Sciences, 109(44), 18192-18197, doi:10.1073/pnas.1209174109

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Abstract:
Calcifying echinoid larvae respond to changes in seawater carbonate chemistry with reduced growth and developmental delay. To date, no information exists on how ocean acidification acts on pH homeostasis in echinoderm larvae. Understanding acid-base regulatory capacities is important because intracellular formation and maintenance of the calcium carbonate skeleton is dependent on pH homeostasis. Using H(+)-selective microelectrodes and the pH-sensitive fluorescent dye BCECF, we conducted in vivo measurements of extracellular and intracellular pH (pH(e) and pH(i)) in echinoderm larvae. We exposed pluteus larvae to a range of seawater CO(2) conditions and demonstrated that the extracellular compartment surrounding the calcifying primary mesenchyme cells (PMCs) conforms to the surrounding seawater with respect to pH during exposure to elevated seawater pCO(2). Using FITC dextran conjugates, we demonstrate that sea urchin larvae have a leaky integument. PMCs and spicules are therefore directly exposed to strong changes in pH(e) whenever seawater pH changes. However, measurements of pH(i) demonstrated that PMCs are able to fully compensate an induced intracellular acidosis. This was highly dependent on Na(+) and HCO(3)(-), suggesting a bicarbonate buffer mechanism involving secondary active Na(+)-dependent membrane transport proteins. We suggest that, under ocean acidification, maintained pH(i) enables calcification to proceed despite decreased pH(e). However, this probably causes enhanced costs. Increased costs for calcification or cellular homeostasis can be one of the main factors leading to modifications in energy partitioning, which then impacts growth and, ultimately, results in increased mortality of echinoid larvae during the pelagic life stage.
Further details:
Lavigne, Héloise; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-05-28.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1SpeciesSpeciesStumpp, Meike
2FigureFigStumpp, Meike
3TreatmentTreatStumpp, Meike
4ReplicateReplicateStumpp, Meike
5pHpHStumpp, MeikeNBS scale
6pH, extracellularpHeStumpp, MeikeNBS scale
7Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetPaStumpp, Meiketreatment
8Molecular massMuStumpp, Meike
9Time in minutesTimeminStumpp, Meike
10FluorescenceFluorescencearbitrary unitsStumpp, Meikeratio of FITC fluorescence within the ECM and the SW surrounding the larva
11Fluorescence, standard deviationFluorescence std dev±Stumpp, Meikeratio of FITC fluorescence within the ECM and the SW surrounding the larva
12Time in secondsTimesStumpp, Meike
13RatioRatioStumpp, Meikedetected emission ratio of the pH-sensitive dye BCECF
14pH, intracellularpH inStumpp, Meike
15RecoveryRec%Stumpp, Meike
16Slope inclinationSlope inc%Stumpp, Meike
17Temperature, waterTemp°CStumpp, Meike
18Temperature, water, standard deviationTemp std dev±Stumpp, Meike
19SalinitySalStumpp, Meike
20Salinity, standard deviationSal std dev±Stumpp, Meike
21pHpHStumpp, Meike
22pH, standard deviationpH std dev±Stumpp, Meike
23Calcite saturation stateOmega CalStumpp, Meike
24Calcite saturation state, standard deviationOmega Cal std dev±Stumpp, Meike
25Aragonite saturation stateOmega ArgStumpp, Meike
26Aragonite saturation state, standard deviationOmega Arg std dev±Stumpp, Meike
27Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetppmvStumpp, Meike
28Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Stumpp, Meike
29Carbon, inorganic, dissolvedDICµmol/kgStumpp, Meike
30Carbon, inorganic, dissolved, standard deviationDIC std dev±Stumpp, Meike
31Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
32pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
33Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
34Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
35Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
36Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
37Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
38Alkalinity, totalATµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
39Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
40Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Size:
41045 data points

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