Data Description

Citation:
Stumpp, Meike; Hu, Marian Y; Melzner, Frank; Gutowska, Magdalena A; Dorey, Narimane; Himmerkus, Nina; Holtmann, Wiebke C; Dupont, Sam; Thorndyke, Mike; Bleich, Markus; Yang, Yan (2012): Experiment: Acidified seawater impacts sea urchin larvae pH regulatory systems relevant for calcification. doi:10.1594/PANGAEA.833111,
Supplement to: Stumpp, Meike; Hu, Marian Y; Melzner, Frank; Gutowska, Magdalena A; Dorey, Narimane; Himmerkus, Nina; Holtmann, Wiebke C; Dupont, Sam; Thorndyke, Mike; Bleich, Markus (2012): Acidified seawater impacts sea urchin larvae pH regulatory systems relevant for calcification. Proceedings of the National Academy of Sciences, 109(44), 18192-18197, doi:10.1073/pnas.1209174109
Abstract:
Calcifying echinoid larvae respond to changes in seawater carbonate chemistry with reduced growth and developmental delay. To date, no information exists on how ocean acidification acts on pH homeostasis in echinoderm larvae. Understanding acid-base regulatory capacities is important because intracellular formation and maintenance of the calcium carbonate skeleton is dependent on pH homeostasis. Using H(+)-selective microelectrodes and the pH-sensitive fluorescent dye BCECF, we conducted in vivo measurements of extracellular and intracellular pH (pH(e) and pH(i)) in echinoderm larvae. We exposed pluteus larvae to a range of seawater CO(2) conditions and demonstrated that the extracellular compartment surrounding the calcifying primary mesenchyme cells (PMCs) conforms to the surrounding seawater with respect to pH during exposure to elevated seawater pCO(2). Using FITC dextran conjugates, we demonstrate that sea urchin larvae have a leaky integument. PMCs and spicules are therefore directly exposed to strong changes in pH(e) whenever seawater pH changes. However, measurements of pH(i) demonstrated that PMCs are able to fully compensate an induced intracellular acidosis. This was highly dependent on Na(+) and HCO(3)(-), suggesting a bicarbonate buffer mechanism involving secondary active Na(+)-dependent membrane transport proteins. We suggest that, under ocean acidification, maintained pH(i) enables calcification to proceed despite decreased pH(e). However, this probably causes enhanced costs. Increased costs for calcification or cellular homeostasis can be one of the main factors leading to modifications in energy partitioning, which then impacts growth and, ultimately, results in increased mortality of echinoid larvae during the pelagic life stage.
Further details:
Lavigne, Héloise; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb *
Project(s):
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-05-28.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Species *SpeciesStumpp, Meike *
2Figure *FigStumpp, Meike *
3Treatment *TreatmStumpp, Meike *
4Replicate *ReplicateStumpp, Meike *
5pH *pHStumpp, Meike *NBS scale
6pH, extracellular *pHeStumpp, Meike *NBS scale
7Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetPaStumpp, Meike *treatment
8Molecular mass *MuStumpp, Meike *
9Time in minutes *TimeminStumpp, Meike *
10Fluorescence *Fluorescencearbitrary unitsStumpp, Meike *ratio of FITC fluorescence within the ECM and the SW surrounding the larva
11Fluorescence, standard deviation *Fluorescence std dev±Stumpp, Meike *ratio of FITC fluorescence within the ECM and the SW surrounding the larva
12Time in seconds *TimesStumpp, Meike *
13Ratio *RatioStumpp, Meike *detected emission ratio of the pH-sensitive dye BCECF
14pH, intracellular *pH inStumpp, Meike *
15Recovery *Rec%Stumpp, Meike *
16Slope inclination *Slope inc%Stumpp, Meike *
17Temperature, water *Temp°CStumpp, Meike *
18Temperature, water, standard deviation *Temp std dev±Stumpp, Meike *
19Salinity *SalStumpp, Meike *
20Salinity, standard deviation *Sal std dev±Stumpp, Meike *
21pH *pHStumpp, Meike *
22pH, standard deviation *pH std dev±Stumpp, Meike *
23Calcite saturation state *Omega CalStumpp, Meike *
24Calcite saturation state, standard deviation *Omega Cal std dev±Stumpp, Meike *
25Aragonite saturation state *Omega ArgStumpp, Meike *
26Aragonite saturation state, standard deviation *Omega Arg std dev±Stumpp, Meike *
27Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetppmvStumpp, Meike *
28Partial pressure of carbon dioxide, standard deviation *pCO2 std dev±Stumpp, Meike *
29Carbon, inorganic, dissolved *DICµmol/kgStumpp, Meike *
30Carbon, inorganic, dissolved, standard deviation *DIC std dev±Stumpp, Meike *
31Carbonate system computation flag *CSC flagYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
32pH *pHYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *total scale
33Carbon dioxide *CO2µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
34Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
35Fugacity of carbon dioxide (water) at sea surface temperature (wet air) *fCO2water_SST_wetµatmYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
36Bicarbonate ion *[HCO3]-µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
37Carbonate ion *[CO3]2-µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
38Alkalinity, total *ATµmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
39Aragonite saturation state *Omega ArgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
40Calcite saturation state *Omega CalYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
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41045 data points

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