Citation:

gsjfr.44.2.76

Name: Impact of changing carbonate chemistry, temperature, and salinity on growth and test degradation of the benthic foraminifer Ammonia aomoriensis
Published: 2014-05-27
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Ammonia aomoriensis; Baltic Sea; Benthos; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Coast and continental shelf; Foraminifera; Growth/Morphology; Heterotrophic prokaryotes; Laboratory experiment; Mortality/Survival; Salinity; Single species; Temperate; Temperature

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Abstract:

The present study investigated the combined effects of ocean acidification, temperature, and salinity on growth and test degradation of Ammonia aomoriensis. This species is one of the dominant benthic foraminifera in near-coastal habitats of the southwestern Baltic Sea that can be particularly sensitive to changes in seawater carbonate chemistry. To assess potential responses to ocean acidification and climate change, we performed a fully crossed experiment involving three temperatures (8, 13, and 18°C), three salinities (15, 20, and 25) and four pCO2 levels (566, 1195, 2108, and 3843 µatm) for six weeks. Our results highlight a sensitive response of A. aomoriensis to undersaturated seawater with respect to calcite. The specimens continued to grow and increase their test diameter in treatments with pCO2 <1200 µatm, when Omega calc >1. Growth rates declined when pCO2 exceeded 1200 µatm (Omega calc <1). A significant reduction in test diameter and number of tests due to dissolution was observed below a critical Omega calc of 0.5. Elevated temperature (18°C) led to increased Omega calc, larger test diameter, and lower test degradation. Maximal growth was observed at 18°C. No significant relationship was observed between salinity and test growth. Lowered and undersaturated Omega calc, which results from increasing pCO2 in bottom waters, may cause a significant future decline of the population density of A. aomoriensis in its natural environment. At the same time, this effect might be partially compensated by temperature rise due to global warming.

Keyword(s):

Ammonia aomoriensis; Baltic Sea; Benthos; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Coast and continental shelf; Foraminifera; Growth/Morphology; Heterotrophic prokaryotes; Laboratory experiment; Mortality/Survival; Salinity; Single species; Temperate; Temperature

Further details:

Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-05-26.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Haynert, Kristin
2	Figure	Fig		Haynert, Kristin
3	Table	Tab		Haynert, Kristin
4	Temperature, water	Temp	°C	Haynert, Kristin
5	Salinity	Sal		Haynert, Kristin
6	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Haynert, Kristin
7	Percentage	Perc	%	Haynert, Kristin		living specimens, with cytoplasm
8	Percentage	Perc	%	Haynert, Kristin		dead tests, without cytoplasm
9	Incubation duration	Inc dur	days	Haynert, Kristin
10	Temperature, water	Temp	°C	Haynert, Kristin
11	Temperature, water, standard deviation	Temp std dev	±	Haynert, Kristin
12	Salinity	Sal		Haynert, Kristin
13	Salinity, standard deviation	Sal std dev	±	Haynert, Kristin
14	pH	pH		Haynert, Kristin	Potentiometric	NBS scale
15	pH, standard deviation	pH std dev	±	Haynert, Kristin	Potentiometric	NBS scale
16	Alkalinity, total	AT	µmol/kg	Haynert, Kristin	Potentiometric titration
17	Alkalinity, total, standard deviation	AT std dev	±	Haynert, Kristin	Potentiometric titration
18	Phosphate	[PO4]3-	µmol/l	Haynert, Kristin	Colorimetric
19	Phosphate, standard deviation	[PO4]3- std dev	±	Haynert, Kristin	Colorimetric
20	Silicate	Si(OH)4	µmol/l	Haynert, Kristin	Colorimetric
21	Silicate, standard deviation	Si(OH)4 std dev	±	Haynert, Kristin	Colorimetric
22	Carbon, inorganic, dissolved	DIC	µmol/kg	Haynert, Kristin	Calculated using CO2SYS
23	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Haynert, Kristin	Calculated using CO2SYS
24	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Haynert, Kristin	Calculated using CO2SYS
25	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Haynert, Kristin	Calculated using CO2SYS
26	Calcite saturation state	Omega Cal		Haynert, Kristin	Calculated using CO2SYS
27	Calcite saturation state, standard deviation	Omega Cal std dev	±	Haynert, Kristin	Calculated using CO2SYS
28	Diameter change	Ø change	µm	Haynert, Kristin
29	Diameter change, standard deviation	Diam change std dev	±	Haynert, Kristin
30	Survival	Survival	%	Haynert, Kristin
31	Survival rate, standard deviation	Survival rate std dev	±	Haynert, Kristin
32	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
34	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

2538 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 Fig	3 Tab	4 Temp [°C]	5 Sal	6 pCO2water_SST_wet [µatm]	7 Perc [%] (living specimens, with cytoplasm)	8 Perc [%] (dead tests, without cytoplasm)	9 Inc dur [days]	10 Temp [°C]	11 Temp std dev [±]	12 Sal	13 Sal std dev [±]	14 pH (NBS scale, Potentiometric)	15 pH std dev [±] (NBS scale, Potentiometric)	16 AT [µmol/kg] (Potentiometric titration)	17 AT std dev [±] (Potentiometric titration)	18 [PO4]3- [µmol/l] (Colorimetric)	19 [PO4]3- std dev [±] (Colorimetric)	20 Si(OH)4 [µmol/l] (Colorimetric)	21 Si(OH)4 std dev [±] (Colorimetric)	22 DIC [µmol/kg] (Calculated using CO2SYS)	23 DIC std dev [±] (Calculated using CO2SYS)	24 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	25 pCO2 std dev [±] (Calculated using CO2SYS)	26 Omega Cal (Calculated using CO2SYS)	27 Omega Cal std dev [±] (Calculated using CO2SYS)	28 Ø change [µm]	29 Diam change std dev [±]	30 Survival [%]	31 Survival rate std dev [±]	32 CSC flag (Calculated using seacarb afte...)	33 pH (total scale, Calculated using...)	34 CO2 [µmol/kg] (Calculated using seacarb afte...)	35 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	36 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	37 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	38 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	39 DIC [µmol/kg] (Calculated using seacarb afte...)	40 Omega Arg (Calculated using seacarb afte...)	41 Omega Cal (Calculated using seacarb afte...)
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	8	15	380	100	0		8.0	0.03	15.0	0.03	8.10	0.00	1833.6	166.8	0.06	0.01	16.93	3.99	1790.4	165.4	481.0	55.2	1.34	0.13	33	8.76	78	5.00	26	8.06	20	383	382	1689	62	1771	0.95	1.64
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	8	15	1120	88	13		8.0	0.03	15.0	0.03	7.77	0.03	1824.9	172.9	0.04	0.01	16.71	4.71	1846.5	166.3	1052.0	49.4	0.66	0.13	-24	12.51	73	5.00	26	7.73	44	840	836	1753	30	1828	0.47	0.81
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	8	15	2400	75	25		8.0	0.03	15.0	0.03	7.60	0.00	1830.6	208.2	0.06	0.01	16.34	4.44	1885.1	211.7	1498.0	115.0	0.47	0.07	-24	12.09	63	5.00	26	7.56	67	1281	1276	1782	21	1870	0.32	0.55
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	8	15	4000	25	75		8.0	0.03	15.0	0.04	7.27	0.00	1832.5	171.8	0.05	0.02	16.22	4.08	2001.7	238.3	3442.0	1324.8	0.22	0.04	-61	17.24	53	5.00	26	7.23	146	2777	2766	1809	10	1965	0.15	0.26
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	8	20	380	100	0		8.0	0.02	20.0	0.02	8.06	0.00	2077.7	37.1	0.25	0.27	26.46	1.87	2018.6	29.0	549.0	38.6	1.61	0.15	32	8.39	73	5.00	26	8.01	23	451	449	1902	74	1998	1.14	1.90
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	8	20	1120	17	83		8.0	0.03	20.0	0.03	7.77	0.00	2083.9	41.5	0.24	0.26	27.05	2.00	2096.8	54.8	1149.0	186.6	0.84	0.10	-7	4.77	70	8.16	26	7.72	47	915	912	1989	40	2075	0.61	1.02
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	8	20	2400	24	76		8.0	0.03	20.0	0.00	7.57	0.00	2093.8	51.0	0.24	0.29	28.00	1.65	2159.4	59.2	1861.0	204.8	0.54	0.05	-25	3.16	60	8.16	26	7.53	75	1466	1460	2032	26	2132	0.40	0.67
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	8	20	4000	0	100		8.0	0.03	20.0	0.03	7.26	0.00	2092.8	44.4	0.26	0.28	26.52	2.39	2261.0	67.2	3618.0	928.7	0.29	0.07	-60	24.33	48	5.00	26	7.22	155	3048	3036	2062	13	2230	0.20	0.33
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	8	25	380	94	6		8.0	0.02	25.1	0.02	8.11	0.00	2387.8	39.7	0.13	0.13	28.75	1.44	2282.0	23.1	521.0	98.8	2.37	0.49	27	14.93	73	5.00	26	8.06	22	437	436	2133	106	2261	1.64	2.67
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	8	25	1120	58	42		8.0	0.02	25.1	0.03	7.77	0.00	2394.3	47.8	0.16	0.12	28.28	2.14	2394.2	53.2	1234.0	147.0	1.10	0.10	-8	10.37	70	8.16	26	7.72	50	1001	997	2268	53	2370	0.81	1.32
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	8	25	2400	75	25		8.0	0.03	25.1	0.03	7.56	0.00	2393.9	38.0	0.14	0.15	28.07	2.15	2454.3	43.1	2011.0	227.0	0.70	0.08	-13	4.86	58	5.00	26	7.51	83	1672	1665	2315	33	2431	0.51	0.82
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	8	25	4000	50	50		8.0	0.03	25.1	0.02	7.26	0.01	2370.9	36.1	0.14	0.10	29.31	2.40	2551.1	87.9	4055.0	1172.2	0.36	0.08	-36	11.59	43	9.57	26	7.21	166	3345	3331	2331	17	2513	0.26	0.42
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	13	15	380	100	0		13.0	0.03	15.0	0.03	8.09	0.00	1829.2	177.2	0.04	0.01	17.16	4.67	1775.9	183.3	530.0	110.5	1.54	0.07	23	4.52	73	9.57	26	8.05	18	401	399	1660	72	1751	1.14	1.94
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	13	15	1120	25	75		13.0	0.01	15.0	0.03	7.80	0.00	1803.3	160.1	0.04	0.01	16.67	3.22	1807.0	153.0	1021.0	38.4	0.83	0.15	-9	1.50	65	5.77	26	7.75	36	814	811	1714	38	1789	0.60	1.02
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	13	15	2400	0	100		13.0	0.02	15.0	0.03	7.54	0.00	1822.4	177.5	0.05	0.03	15.69	3.27	1882.9	173.1	1896.0	104.5	0.48	0.10	-18	2.96	58	9.57	26	7.49	68	1521	1516	1772	22	1861	0.34	0.58
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	13	15	4000	33	67		13.0	0.15	15.0	0.03	7.26	0.00	1827.3	175.3	0.05	0.01	14.87	4.09	1977.0	234.2	3662.0	1324.4	0.26	0.04	-40	8.56	55	5.77	26	7.21	133	2997	2986	1801	11	1945	0.18	0.31
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	13	20	380	100	0		12.9	0.00	20.1	0.03	8.05	0.19	2064.0	49.3	0.23	0.28	24.46	1.94	1998.7	10.4	685.0	316.1	1.80	0.58	35	6.04	83	12.58	26	7.99	21	477	476	1863	85	1969	1.33	2.19
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	13	20	1120	100	0		13.0	0.03	20.1	0.03	7.86	0.19	2090.3	54.5	0.21	0.27	24.38	2.38	2098.5	57.9	1324.0	74.7	0.93	0.00	-6	6.74	68	5.00	26	7.81	33	768	765	1953	58	2044	0.91	1.50
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	13	20	2400	100	0		13.0	0.02	20.1	0.00	7.53	0.02	2120.5	69.9	0.26	0.27	27.48	2.97	2180.5	85.6	2140.0	326.8	0.61	0.06	-17	5.42	68	9.57	26	7.48	75	1733	1727	2053	28	2156	0.45	0.74
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	13	20	4000	0	100		13.0	0.00	20.1	0.02	7.25	0.00	2075.9	55.1	0.22	0.25	24.41	3.00	2230.1	62.5	3972.0	906.8	0.33	0.07	-59	18.89	58	5.00	26	7.20	141	3258	3246	2040	15	2196	0.23	0.39
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	13	25	380	100	0		13.0	0.02	25.0	0.00	8.07	0.01	2362.0	39.8	0.13	0.10	26.15	3.15	2250.2	51.5	596.0	75.6	2.50	0.21	39	1.02	90	8.16	26	8.01	21	490	489	2089	115	2224	1.80	2.90
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	13	25	1120	75	25		13.0	0.00	25.1	0.00	7.74	0.00	2408.1	31.5	0.11	0.12	27.01	3.58	2395.5	26.1	1355.0	155.7	1.27	0.16	-13	4.86	75	5.77	26	7.68	48	1153	1149	2271	58	2377	0.90	1.46
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	13	25	2400	67	33		13.0	0.03	25.0	0.00	7.55	0.00	2390.6	31.8	0.09	0.10	25.86	2.85	2436.3	38.6	2154.0	264.7	0.82	0.09	-12	7.11	68	9.57	26	7.49	77	1822	1816	2301	37	2415	0.59	0.95
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	13	25	4000	0	100		13.0	0.02	25.0	0.03	7.27	0.00	2381.9	53.6	0.10	0.10	25.73	3.93	2521.5	75.9	3924.0	734.5	0.46	0.07	-45	7.73	63	9.57	26	7.21	147	3499	3486	2334	20	2501	0.31	0.51
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	18	15	380	60	40		18.1	0.03	15.2	0.03	8.01	0.01	1937.3	6.3	0.65	0.87	1.14	1.54	1875.5	11.5	656.0	69.5	1.80	0.16	92	55.33	53	5.00	26	7.95	21	551	549	1763	75	1860	1.22	2.06
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	18	15	1120	55	45		18.1	0.04	15.1	0.03	7.74	0.02	1916.3	28.7	0.60	0.80	0.75	1.01	1911.9	25.0	1202.0	55.0	1.03	0.07	44	6.82	60	8.16	26	7.68	40	1053	1049	1819	42	1901	0.68	1.14
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	18	15	2400	0	100		18.1	0.06	15.1	0.01	7.51	0.01	1890.3	52.0	0.60	0.79	1.65	2.32	1946.8	57.5	2227.0	182.7	0.56	0.01	-8	19.15	80	14.14	26	7.46	68	1777	1770	1832	25	1924	0.41	0.69
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	18	15	4000	100	0		18.1	0.03	15.1	0.02	7.25	0.01	1895.0	53.6	0.61	0.82	1.69	2.37	2027.4	54.4	3936.0	564.0	0.33	0.05	-28	15.34	38	17.08	26	7.19	128	3357	3345	1863	14	2004	0.22	0.38
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	18	20	380	85	15		18.1	0.03	20.1	0.03	8.05	0.01	1779.6	0.3	0.68	0.92	2.07	2.49	1696.0	8.5	526.0	46.7	1.91	0.15	65	18.31	70	14.14	26	7.99	16	422	421	1571	86	1673	1.39	2.27
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	18	20	1120	82	18		18.1	0.06	20.1	0.03	7.73	0.01	1778.0	11.0	0.71	0.95	2.16	2.70	1770.4	11.4	1179.0	53.3	0.94	0.04	35	11.42	68	26.30	26	7.66	35	950	947	1673	43	1751	0.70	1.14
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	18	20	2400	0	100		18.1	0.03	20.1	0.03	7.41	0.01	1788.9	7.4	0.70	0.93	2.02	2.47	1855.2	11.9	2493.0	169.3	0.47	0.03	9	8.13	75	10.00	26	7.35	76	2054	2047	1736	22	1834	0.35	0.57
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	18	20	4000	100	0		18.1	0.00	20.1	0.05	7.20	0.01	1773.1	4.9	0.71	0.95	2.01	2.71	1896.4	19.1	3795.0	419.6	0.31	0.03	-14	7.16	58	9.57	26	7.14	124	3342	3331	1740	13	1877	0.21	0.35
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	18	25	380	67	33		18.1	0.02	25.1	0.02	8.10	0.00	2232.3	53.2	0.58	0.78	1.91	2.25	2093.5	55.3	551.0	41.4	2.90	0.12	31	12.07	65	5.77	26	8.02	16	456	455	1918	131	2066	2.10	3.35
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	18	25	1120	50	50		18.1	0.03	25.1	0.02	7.77	0.01	2245.2	18.4	0.57	0.76	1.60	2.04	2208.4	20.1	1240.0	22.6	1.48	0.01	30	16.53	63	12.58	26	7.69	38	1058	1054	2085	67	2190	1.07	1.71
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	18	25	2400	22	78		18.0	0.02	25.1	0.00	7.44	0.01	2266.2	1.6	0.57	0.76	1.56	2.19	2322.2	12.7	2692.0	242.9	0.75	0.06	-15	4.19	73	9.57	26	7.37	84	2329	2321	2186	33	2304	0.53	0.85
Ammonia aomoriensis (protist)	4, 5 and 6	1, 3	18	25	4000	0	100		18.0	0.03	25.1	0.02	7.25	0.01	2258.0	0.6	0.56	0.75	1.33	1.87	2382.1	10.9	4185.0	228.3	0.49	0.02	-24	11.07	50	11.55	26	7.18	132	3649	3637	2206	22	2359	0.35	0.55
Ammonia aomoriensis (protist)	5 (Haynert et al. 2011)		13	18	380				11.8	0.60	18.4	0.80	7.90	0.05	2040.1	20.7	0.99	0.33			1980.2	31.8	617.9	8.5	1.66	0.25	23	5.40			26	7.85	31	688	686	1907	56	1994	0.88	1.47
Ammonia aomoriensis (protist)	5 (Haynert et al. 2011)		13	18	1120				11.7	0.60	18.4	0.80	7.81	0.04	2039.0	17.4	1.02	0.32			1999.5	22.3	929.1	23.4	1.14	0.13	21	4.44			26	7.76	39	856	853	1929	46	2015	0.72	1.21
Ammonia aomoriensis (protist)	5 (Haynert et al. 2011)		13	18	2400				11.6	0.60	18.4	0.80	7.43	0.04	2036.2	21.5	1.01	0.30			2075.2	20.4	1829.2	33.5	0.64	0.08	-3	5.80			26	7.38	96	2114	2106	1989	20	2105	0.31	0.52
Ammonia aomoriensis (protist)	5 (Haynert et al. 2011)		13	18	4000				11.9	0.50	18.4	0.80	7.19	0.04	2039.7	19.2	1.00	0.33			2156.2	24.5	3130.2	33.6	0.37	0.04	-37	6.51			26	7.14	168	3729	3715	2012	12	2192	0.18	0.30
Ammonia aomoriensis (protist)	2		8	15				0	8.0	0.01
Ammonia aomoriensis (protist)	2		8	15				7	8.0	0.02
Ammonia aomoriensis (protist)	2		8	15				14	8.0	0.02
Ammonia aomoriensis (protist)	2		8	15				21	8.0	0.03
Ammonia aomoriensis (protist)	2		8	15				28	8.1	0.00
Ammonia aomoriensis (protist)	2		8	15				34	8.1	0.03
Ammonia aomoriensis (protist)	2		8	15				41	8.1	0.02
Ammonia aomoriensis (protist)	2		8	20				0	8.0	0.04
Ammonia aomoriensis (protist)	2		8	20				7	8.0	0.01
Ammonia aomoriensis (protist)	2		8	20				14	8.0	0.03
Ammonia aomoriensis (protist)	2		8	20				21	8.0	0.03
Ammonia aomoriensis (protist)	2		8	20				28	8.1	0.03
Ammonia aomoriensis (protist)	2		8	20				34	8.1	0.03
Ammonia aomoriensis (protist)	2		8	20				41	8.1	0.03
Ammonia aomoriensis (protist)	2		8	25				0	8.0	0.03
Ammonia aomoriensis (protist)	2		8	25				7	8.0	0.03
Ammonia aomoriensis (protist)	2		8	25				14	8.0	0.01
Ammonia aomoriensis (protist)	2		8	25				21	8.0	0.03
Ammonia aomoriensis (protist)	2		8	25				28	8.1	0.03
Ammonia aomoriensis (protist)	2		8	25				34	8.1	0.00
Ammonia aomoriensis (protist)	2		8	25				41	8.1	0.03
Ammonia aomoriensis (protist)	2		8	15				0			15.0	0.00
Ammonia aomoriensis (protist)	2		8	15				7			14.8	0.03
Ammonia aomoriensis (protist)	2		8	15				14			15.0	0.03
Ammonia aomoriensis (protist)	2		8	15				21			15.0	0.03
Ammonia aomoriensis (protist)	2		8	15				28			15.1	0.03
Ammonia aomoriensis (protist)	2		8	15				34			15.1	0.04
Ammonia aomoriensis (protist)	2		8	15				41			15.1	0.03
Ammonia aomoriensis (protist)	2		8	20				0			20.0	0.03
Ammonia aomoriensis (protist)	2		8	20				7			20.1	0.03
Ammonia aomoriensis (protist)	2		8	20				14			20.0	0.00
Ammonia aomoriensis (protist)	2		8	20				21			20.0	0.03
Ammonia aomoriensis (protist)	2		8	20				28			20.1	0.03
Ammonia aomoriensis (protist)	2		8	20				34			20.0	0.00
Ammonia aomoriensis (protist)	2		8	20				41			20.1	0.03
Ammonia aomoriensis (protist)	2		8	25				0			25.0	0.03
Ammonia aomoriensis (protist)	2		8	25				7			25.2	0.03
Ammonia aomoriensis (protist)	2		8	25				14			25.1	0.00
Ammonia aomoriensis (protist)	2		8	25				21			25.0	0.03
Ammonia aomoriensis (protist)	2		8	25				28			25.0	0.03
Ammonia aomoriensis (protist)	2		8	25				34			25.1	0.00
Ammonia aomoriensis (protist)	2		8	25				41			25.0	0.03
Ammonia aomoriensis (protist)	2		8	15	380			0					8.08	0.00
Ammonia aomoriensis (protist)	2		8	15	380			7					8.11	0.00
Ammonia aomoriensis (protist)	2		8	15	380			14					8.11	0.00
Ammonia aomoriensis (protist)	2		8	15	380			21					8.09	0.00
Ammonia aomoriensis (protist)	2		8	15	380			28					8.12	0.01
Ammonia aomoriensis (protist)	2		8	15	380			34					8.11	0.01
Ammonia aomoriensis (protist)	2		8	15	380			41					8.06	0.01
Ammonia aomoriensis (protist)	2		8	20	380			0					8.02	0.00
Ammonia aomoriensis (protist)	2		8	20	380			7					7.94	0.00
Ammonia aomoriensis (protist)	2		8	20	380			14					8.04	0.01
Ammonia aomoriensis (protist)	2		8	20	380			21					8.13	0.00
Ammonia aomoriensis (protist)	2		8	20	380			28					8.09	0.00
Ammonia aomoriensis (protist)	2		8	20	380			34					8.12	0.00
Ammonia aomoriensis (protist)	2		8	20	380			41					8.08	0.01
Ammonia aomoriensis (protist)	2		8	25	380			0					8.09	0.01
Ammonia aomoriensis (protist)	2		8	25	380			7					7.99	0.01
Ammonia aomoriensis (protist)	2		8	25	380			14					8.03	0.01
Ammonia aomoriensis (protist)	2		8	25	380			21					8.15	0.01
Ammonia aomoriensis (protist)	2		8	25	380			28					8.23	0.01
Ammonia aomoriensis (protist)	2		8	25	380			34					8.17	0.01
Ammonia aomoriensis (protist)	2		8	25	380			41					8.08	0.00
Ammonia aomoriensis (protist)	2		8	15	1120			0					7.82	0.00
Ammonia aomoriensis (protist)	2		8	15	1120			7					7.82	0.08
Ammonia aomoriensis (protist)	2		8	15	1120			14					7.79	0.01
Ammonia aomoriensis (protist)	2		8	15	1120			21					7.78	0.01
Ammonia aomoriensis (protist)	2		8	15	1120			28					7.73	0.01
Ammonia aomoriensis (protist)	2		8	15	1120			34					7.75	0.00
Ammonia aomoriensis (protist)	2		8	15	1120			41					7.72	0.01
Ammonia aomoriensis (protist)	2		8	20	1120			0					7.82	0.00
Ammonia aomoriensis (protist)	2		8	20	1120			7					7.85	0.00
Ammonia aomoriensis (protist)	2		8	20	1120			14					7.79	0.00
Ammonia aomoriensis (protist)	2		8	20	1120			21					7.77	0.00
Ammonia aomoriensis (protist)	2		8	20	1120			28					7.71	0.01
Ammonia aomoriensis (protist)	2		8	20	1120			34					7.75	0.00
Ammonia aomoriensis (protist)	2		8	20	1120			41					7.70	0.00
Ammonia aomoriensis (protist)	2		8	25	1120			0					7.80	0.00
Ammonia aomoriensis (protist)	2		8	25	1120			7					7.85	0.00
Ammonia aomoriensis (protist)	2		8	25	1120			14					7.75	0.01
Ammonia aomoriensis (protist)	2		8	25	1120			21					7.80	0.01
Ammonia aomoriensis (protist)	2		8	25	1120			28					7.78	0.02
Ammonia aomoriensis (protist)	2		8	25	1120			34					7.75	0.00
Ammonia aomoriensis (protist)	2		8	25	1120			41					7.70	0.01
Ammonia aomoriensis (protist)	2		8	15	2400			0					7.64	0.00
Ammonia aomoriensis (protist)	2		8	15	2400			7					7.64	0.00
Ammonia aomoriensis (protist)	2		8	15	2400			14					7.53	0.00
Ammonia aomoriensis (protist)	2		8	15	2400			21					7.57	0.00
Ammonia aomoriensis (protist)	2		8	15	2400			28					7.62	0.01
Ammonia aomoriensis (protist)	2		8	15	2400			34					7.58	0.01
Ammonia aomoriensis (protist)	2		8	15	2400			41					7.60	0.01
Ammonia aomoriensis (protist)	2		8	20	2400			0					7.61	0.00
Ammonia aomoriensis (protist)	2		8	20	2400			7					7.63	0.01
Ammonia aomoriensis (protist)	2		8	20	2400			14					7.53	0.01
Ammonia aomoriensis (protist)	2		8	20	2400			21					7.59	0.00
Ammonia aomoriensis (protist)	2		8	20	2400			28					7.52	0.01
Ammonia aomoriensis (protist)	2		8	20	2400			34					7.60	0.01
Ammonia aomoriensis (protist)	2		8	20	2400			41					7.55	0.00
Ammonia aomoriensis (protist)	2		8	25	2400			0					7.62	0.00
Ammonia aomoriensis (protist)	2		8	25	2400			7					7.66	0.00
Ammonia aomoriensis (protist)	2		8	25	2400			14					7.52	0.01
Ammonia aomoriensis (protist)	2		8	25	2400			21					7.56	0.01
Ammonia aomoriensis (protist)	2		8	25	2400			28					7.53	0.01
Ammonia aomoriensis (protist)	2		8	25	2400			34					7.50	0.00
Ammonia aomoriensis (protist)	2		8	25	2400			41					7.53	0.00
Ammonia aomoriensis (protist)	2		8	15	4000			0					7.12	0.01
Ammonia aomoriensis (protist)	2		8	15	4000			7					7.12	0.01
Ammonia aomoriensis (protist)	2		8	15	4000			14					7.38	0.00
Ammonia aomoriensis (protist)	2		8	15	4000			21					7.32	0.01
Ammonia aomoriensis (protist)	2		8	15	4000			28					7.32	0.01
Ammonia aomoriensis (protist)	2		8	15	4000			34					7.30	0.01
Ammonia aomoriensis (protist)	2		8	15	4000			41					7.31	0.01
Ammonia aomoriensis (protist)	2		8	20	4000			0					7.13	0.00
Ammonia aomoriensis (protist)	2		8	20	4000			7					7.21	0.00
Ammonia aomoriensis (protist)	2		8	20	4000			14					7.39	0.01
Ammonia aomoriensis (protist)	2		8	20	4000			21					7.21	0.00
Ammonia aomoriensis (protist)	2		8	20	4000			28					7.29	0.01
Ammonia aomoriensis (protist)	2		8	20	4000			34					7.31	0.01
Ammonia aomoriensis (protist)	2		8	20	4000			41					7.30	0.01
Ammonia aomoriensis (protist)	2		8	25	4000			0					7.10	0.00
Ammonia aomoriensis (protist)	2		8	25	4000			7					7.18	0.00
Ammonia aomoriensis (protist)	2		8	25	4000			14					7.37	0.03
Ammonia aomoriensis (protist)	2		8	25	4000			21					7.30	0.01
Ammonia aomoriensis (protist)	2		8	25	4000			28					7.29	0.02
Ammonia aomoriensis (protist)	2		8	25	4000			34					7.32	0.01
Ammonia aomoriensis (protist)	2		8	25	4000			41					7.28	0.01