Data Description

Citation:
Lohbeck, KT et al. (2014): Gene expression changes in the coccolithophore Emiliania huxleyi after 500 generations of selection to ocean acidification. doi:10.1594/PANGAEA.832536,
Supplement to: Lohbeck, Kai T; Riebesell, Ulf; Reusch, Thorsten BH (2014): Gene expression changes in the coccolithophore Emiliania huxleyi after 500 generations of selection to ocean acidification. Proceedings of the Royal Society B-Biological Sciences, 281(1786), 20140003-20140003, doi:10.1098/rspb.2014.0003
Abstract:
Coccolithophores are unicellular marine algae that produce biogenic calcite scales and substantially contribute to marine primary production and carbon export to the deep ocean. Ongoing ocean acidification particularly impairs calcifying organisms, mostly resulting in decreased growth and calcification. Recent studies revealed that the immediate physiological response in the coccolithophore Emiliania huxleyi to ocean acidification may be partially compensated by evolutionary adaptation, yet the underlying molecular mechanisms are currently unknown. Here, we report on the expression levels of 10 candidate genes putatively relevant to pH regulation, carbon transport, calcification and photosynthesis in E. huxleyi populations short-term exposed to ocean acidification conditions after acclimation (physiological response) and after 500 generations of high CO2 adaptation (adaptive response). The physiological response revealed downregulation of candidate genes, well reflecting the concomitant decrease of growth and calcification. In the adaptive response, putative pH regulation and carbon transport genes were up-regulated, matching partial restoration of growth and calcification in high CO2-adapted populations. Adaptation to ocean acidification in E. huxleyi likely involved improved cellular pH regulation, presumably indirectly affecting calcification. Adaptive evolution may thus have the potential to partially restore cellular pH regulatory capacity and thereby mitigate adverse effects of ocean acidification.
Further details:
Lavigne, Héloise; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb *
Project(s):
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-05-15.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Sample code/label *LabelLohbeck, Kai T *
2Species *SpeciesLohbeck, Kai T *
3Experiment *ExpLohbeck, Kai T *
4Treatment *TreatmLohbeck, Kai T *Selection condition (µatm pCO2)
5Salinity *SalLohbeck, Kai T *Selection condition
6Temperature, water *Temp°CLohbeck, Kai T *Selection condition
7Phosphate *PHSPHTµmol/kgLohbeck, Kai T *Selection condition
8Alkalinity, total *ATµmol/kgLohbeck, Kai T *Selection condition
9Carbon, inorganic, dissolved *DICµmol/kgLohbeck, Kai T *Selection condition
10pH *pHLohbeck, Kai T *Selection condition, total scale
11Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmLohbeck, Kai T *Selection condition
12Treatment *TreatmLohbeck, Kai T *Assay condition (µatm pCO2)
13Salinity *SalLohbeck, Kai T *Assay condition
14Temperature, water *Temp°CLohbeck, Kai T *Assay condition
15Phosphate *PHSPHTµmol/kgLohbeck, Kai T *Assay condition
16Alkalinity, total *ATµmol/kgLohbeck, Kai T *Assay condition
17Carbon, inorganic, dissolved *DICµmol/kgLohbeck, Kai T *Assay condition
18pH *pHLohbeck, Kai T *Assay condition, total scale
19Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmLohbeck, Kai T *Assay condition
20Gene name *GeneLohbeck, Kai T *Assay condition
21Threshold cycle *CtLohbeck, Kai T *Threshold cycle_a
22Threshold cycle *CtLohbeck, Kai T *Threshold cycle_b
23Threshold cycle *CtLohbeck, Kai T *Threshold cycle_c
24Carbonate system computation flag *CSC flagYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
25pH *pHYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *Selection condition, total scale
26Carbon dioxide *CO2µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *Selection condition
27Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *Selection condition
28Fugacity of carbon dioxide (water) at sea surface temperature (wet air) *fCO2water_SST_wetµatmYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *Selection condition
29Bicarbonate ion *[HCO3]-µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *Selection condition
30Carbonate ion *[CO3]2-µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *Selection condition
31Aragonite saturation state *Omega ArgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *Selection condition
32Calcite saturation state *Omega CalYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *Selection condition
33pH *pHYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *Assay condition, total scale
34Carbon dioxide *CO2µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *Assay condition
35Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *Assay condition
36Fugacity of carbon dioxide (water) at sea surface temperature (wet air) *fCO2water_SST_wetµatmYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *Assay condition
37Bicarbonate ion *[HCO3]-µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *Assay condition
38Carbonate ion *[CO3]2-µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *Assay condition
39Aragonite saturation state *Omega ArgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *Assay condition
40Calcite saturation state *Omega CalYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *Assay condition
License:
Size:
15400 data points

Download Data

Download dataset as tab-delimited text (use the following character encoding: )

View dataset as HTML (shows only first 2000 rows)