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Lohbeck, Kai T; Riebesell, Ulf; Reusch, Thorsten BH (2014): Gene expression changes in the coccolithophore Emiliania huxleyi after 500 generations of selection to ocean acidification. doi:10.1594/PANGAEA.832536,
Supplement to: Lohbeck, KT et al. (2014): Gene expression changes in the coccolithophore Emiliania huxleyi after 500 generations of selection to ocean acidification. Proceedings of the Royal Society B-Biological Sciences, 281(1786), 20140003-20140003, doi:10.1098/rspb.2014.0003

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Abstract:
Coccolithophores are unicellular marine algae that produce biogenic calcite scales and substantially contribute to marine primary production and carbon export to the deep ocean. Ongoing ocean acidification particularly impairs calcifying organisms, mostly resulting in decreased growth and calcification. Recent studies revealed that the immediate physiological response in the coccolithophore Emiliania huxleyi to ocean acidification may be partially compensated by evolutionary adaptation, yet the underlying molecular mechanisms are currently unknown. Here, we report on the expression levels of 10 candidate genes putatively relevant to pH regulation, carbon transport, calcification and photosynthesis in E. huxleyi populations short-term exposed to ocean acidification conditions after acclimation (physiological response) and after 500 generations of high CO2 adaptation (adaptive response). The physiological response revealed downregulation of candidate genes, well reflecting the concomitant decrease of growth and calcification. In the adaptive response, putative pH regulation and carbon transport genes were up-regulated, matching partial restoration of growth and calcification in high CO2-adapted populations. Adaptation to ocean acidification in E. huxleyi likely involved improved cellular pH regulation, presumably indirectly affecting calcification. Adaptive evolution may thus have the potential to partially restore cellular pH regulatory capacity and thereby mitigate adverse effects of ocean acidification.
Further details:
Lavigne, Héloise; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-05-15.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Sample code/labelLabelLohbeck, Kai T
2SpeciesSpeciesLohbeck, Kai T
3ExperimentExpLohbeck, Kai T
4TreatmentTreatLohbeck, Kai TSelection condition (µatm pCO2)
5SalinitySalLohbeck, Kai TSelection condition
6Temperature, waterTemp°CLohbeck, Kai TSelection condition
7PhosphatePHSPHTµmol/kgLohbeck, Kai TSelection condition
8Alkalinity, totalATµmol/kgLohbeck, Kai TSelection condition
9Carbon, inorganic, dissolvedDICµmol/kgLohbeck, Kai TSelection condition
10pHpHLohbeck, Kai TSelection condition, total scale
11Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmLohbeck, Kai TSelection condition
12TreatmentTreatLohbeck, Kai TAssay condition (µatm pCO2)
13SalinitySalLohbeck, Kai TAssay condition
14Temperature, waterTemp°CLohbeck, Kai TAssay condition
15PhosphatePHSPHTµmol/kgLohbeck, Kai TAssay condition
16Alkalinity, totalATµmol/kgLohbeck, Kai TAssay condition
17Carbon, inorganic, dissolvedDICµmol/kgLohbeck, Kai TAssay condition
18pHpHLohbeck, Kai TAssay condition, total scale
19Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmLohbeck, Kai TAssay condition
20Gene nameGeneLohbeck, Kai TAssay condition
21Threshold cycleCtLohbeck, Kai TThreshold cycle_a
22Threshold cycleCtLohbeck, Kai TThreshold cycle_b
23Threshold cycleCtLohbeck, Kai TThreshold cycle_c
24Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
25pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)Selection condition, total scale
26Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)Selection condition
27Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)Selection condition
28Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)Selection condition
29Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)Selection condition
30Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)Selection condition
31Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)Selection condition
32Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)Selection condition
33pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)Assay condition, total scale
34Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)Assay condition
35Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)Assay condition
36Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)Assay condition
37Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)Assay condition
38Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)Assay condition
39Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)Assay condition
40Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)Assay condition
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15400 data points

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