Citation:

rspb.2013.0155

Name: Seawater carbonate chemistry, physiology, morphology of larval sea urchins, Strongylocentrotus purpuratus in a laboratory experiment
Published: 2013
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Development; Echinodermata; Growth/Morphology; Laboratory experiment; North Pacific; Pelagos; Reproduction; Respiration; Single species; Strongylocentrotus purpuratus; Temperate; Temperature; Zooplankton

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Abstract:

Ocean warming and ocean acidification, both consequences of anthropogenic production of CO2, will combine to influence the physiological performance of many species in the marine environment. In this study, we used an integrative approach to forecast the impact of future ocean conditions on larval purple sea urchins (Strongylocentrotus purpuratus) from the northeast Pacific Ocean. In laboratory experiments that simulated ocean warming and ocean acidification, we examined larval development, skeletal growth, metabolism and patterns of gene expression using an orthogonal comparison of two temperature (13°C and 18°C) and pCO2 (400 and 1100 µatm) conditions. Simultaneous exposure to increased temperature and pCO2 significantly reduced larval metabolism and triggered a widespread downregulation of histone encoding genes. pCO2 but not temperature impaired skeletal growth and reduced the expression of a major spicule matrix protein, suggesting that skeletal growth will not be further inhibited by ocean warming. Importantly, shifts in skeletal growth were not associated with developmental delay. Collectively, our results indicate that global change variables will have additive effects that exceed thresholds for optimized physiological performance in this keystone marine species.

Keyword(s):

Animalia; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Development; Echinodermata; Growth/Morphology; Laboratory experiment; North Pacific; Pelagos; Reproduction; Respiration; Single species; Strongylocentrotus purpuratus; Temperate; Temperature; Zooplankton

Further details:

Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 34.414030 * Longitude: -119.984300

Date/Time Start: 2011-11-01T00:00:00 * Date/Time End: 2011-11-30T00:00:00

Minimum Elevation: -5.0 m * Maximum Elevation: -5.0 m

Event(s):

Santa_Barbara_Channel * Latitude: 34.414030 * Longitude: -119.984300 * Date/Time Start: 2011-11-01T00:00:00 * Date/Time End: 2011-11-30T00:00:00 * Elevation: -5.0 m * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-04-09.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Padilla-Gamiño, Jacqueline L
2	Temperature, water	Temp	°C	Padilla-Gamiño, Jacqueline L		treatment
3	Treatment	Treat		Padilla-Gamiño, Jacqueline L
4	Identification	ID		Padilla-Gamiño, Jacqueline L
5	Length	l	mm	Padilla-Gamiño, Jacqueline L		skeletal
6	Length	l	mm	Padilla-Gamiño, Jacqueline L		arm
7	Length	l	mm	Padilla-Gamiño, Jacqueline L		perpendicular
8	Area in square milimeter	Area	mm²	Padilla-Gamiño, Jacqueline L		stomach
9	Respiration rate, oxygen, per individual	Resp O2/ind	pmol/#/h	Padilla-Gamiño, Jacqueline L		per larva
10	Duration	Duration	h	Padilla-Gamiño, Jacqueline L		hours after fertilization
11	Eggs	Eggs	#	Padilla-Gamiño, Jacqueline L		gastrula
12	Eggs	Eggs	#	Padilla-Gamiño, Jacqueline L		not gastrula
13	Eggs	Eggs	#	Padilla-Gamiño, Jacqueline L		pluteus
14	Eggs	Eggs	#	Padilla-Gamiño, Jacqueline L		not pluteus
15	Temperature, water	Temp	°C	Padilla-Gamiño, Jacqueline L
16	Salinity	Sal		Padilla-Gamiño, Jacqueline L
17	pH	pH		Padilla-Gamiño, Jacqueline L	Potentiometric	total scale
18	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Padilla-Gamiño, Jacqueline L	Calculated using CO2calc
19	Calcite saturation state	Omega Cal		Padilla-Gamiño, Jacqueline L	Calculated using CO2calc
20	Aragonite saturation state	Omega Arg		Padilla-Gamiño, Jacqueline L	Calculated using CO2calc
21	Alkalinity, total	AT	µmol/kg	Padilla-Gamiño, Jacqueline L	Potentiometric titration
22	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
23	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
24	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
25	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
26	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
27	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
28	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
29	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

4571 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 Temp [°C] (treatment)	3 Treat	4 ID	5 l [mm] (skeletal)	6 l [mm] (arm)	7 l [mm] (perpendicular)	8 Area [mm²] (stomach)	9 Resp O2/ind [pmol/#/h] (per larva)	10 Duration [h] (hours after fertilization)	11 Eggs [#] (gastrula)	12 Eggs [#] (not gastrula)	13 Eggs [#] (pluteus)	14 Eggs [#] (not pluteus)	15 Temp [°C]	16 Sal	17 pH (total scale, Potentiometric)	18 pCO2water_SST_wet [µatm] (Calculated using CO2calc)	19 Omega Cal (Calculated using CO2calc)	20 Omega Arg (Calculated using CO2calc)	21 AT [µmol/kg] (Potentiometric titration)	22 CSC flag (Calculated using seacarb afte...)	23 CO2 [µmol/kg] (Calculated using seacarb afte...)	24 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	25 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	26 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	27 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	28 DIC [µmol/kg] (Calculated using seacarb afte...)	29 Omega Arg (Calculated using seacarb afte...)	30 Omega Cal (Calculated using seacarb afte...)
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-11	0.285	0.115	0.218	0.002435154	8.00						13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-12	0.298	0.123	0.221	0.001957000	7.00						13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-13	0.296	0.119	0.229	0.002202600							13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-14	0.281	0.125	0.212	0.002291357	8.30						13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-15	0.294				7.00						13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-16	0.305				7.20						13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-17	0.286										13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-18	0.285				10.00						13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-11	0.329	0.141	0.246	0.002345750	7.50						13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-12	0.339	0.156	0.256	0.001838750	7.20						13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-13	0.322	0.136	0.243	0.001953000							13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-14	0.286	0.126	0.219	0.002050000	7.40						13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-15	0.313				8.90						13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-16	0.325				10.70						13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-17	0.312										13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-18	0.318				7.70						13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-11	0.290	0.111	0.214	0.002220545	8.90						18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-12	0.303	0.123	0.222	0.002092776	10.30						18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-13	0.292										18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-14	0.293	0.123	0.221	0.002095857	7.70						18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-15	0.277	0.113	0.215	0.002135077	10.20						18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-16	0.297	0.121	0.228	0.002105857	13.05						18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-17	0.259										18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-18	0.288	0.116	0.225	0.002360909	8.30						18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-11	0.328	0.145	0.235	0.002263250	15.60						18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-12	0.331	0.155	0.235	0.002138471	11.30						18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-13	0.316										18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-14	0.329	0.147	0.237	0.001990429	11.20						18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-15	0.278	0.103	0.212	0.002405182	10.80						18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-16	0.336				11.50						18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-17	0.319										18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-18	0.326	0.136	0.249	0.001953944	16.06						18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-1						28.00000000	4	46			18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-1						29.65000000	0	54			18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-1						31.38333333	0	52			18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-1						33.68333333	4	51			18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-1						35.78333333	50	0			18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-2						26.81666667	3	47			18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-2						28.68333333	9	54			18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-2						30.51666667	6	51			18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-2						32.65000000	18	38			18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-2						34.71666667	50	0			18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-3						26.51666667	7	52			18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-3						28.53333333	3	50			18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-3						30.10000000	5	49			18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-3						32.06666667	11	47			18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-3						34.25000000	50	0			18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-4						25.98333333	0	60			18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-4						27.96666667	2	50			18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-4						29.43333333	0	62			18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-4						31.50000000	22	33			18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-4						33.63333333	50	0			18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-1						28.00000000	0	48			18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-1						29.55000000	2	53			18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-1						31.55000000	1	55			18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-1						33.55000000	4	55			18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-1						34.75000000	50	0			18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-2						26.75000000	6	45			18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-2						28.53333333	0	54			18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-2						30.65000000	1	67			18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-2						32.53333333	15	39			18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-2						34.66666667	50	0			18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-3						26.05000000	16	40			18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-3						28.41666667	2	51			18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-3						29.90000000	11	40			18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-3						32.00000000	1	52			18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-3						34.16666667	50	0			18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-4						25.43333333	25	27			18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-4						27.88333333	1	59			18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-4						29.56666667	1	58			18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-4						31.40000000	11	42			18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-4						33.58333333	50	0			18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-1						37.08333333	5	47			13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-1						39.38333333	15	39			13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-1						42.33333333	23	27			13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-1						46.25000000	50	0			13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-2						36.50000000	0	52			13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-2						38.30000000	12	43			13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-2						41.25000000	26	31			13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-2						45.16666667	50	0			13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-3						35.08333333	0	50			13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-3						38.23333333	5	47			13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-3						40.50000000	0	50			13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-3						44.33333333	50	0			13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-4						34.60000000	0	50			13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-4						37.71666667	3	49			13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-4						39.85000000	0	48			13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-4						43.85000000	50	0			13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-1						37.00000000	23	29			13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-1						39.30000000	13	39			13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-1						42.25000000	50	0			13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-1						46.16666667	50	0			13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-2						36.50000000	4	47			13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-2						38.21666667	14	39			13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-2						41.16666667	50	0			13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-2						45.08333333	50	0			13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-3						35.01666667	0	59			13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-3						38.18333333	18	36			13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-3						40.33333333	12	36			13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-3						44.25000000	50	0			13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-4						34.60000000	0	61			13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-4						37.71666667	7	46			13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-4						39.85000000	0	50			13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-4						43.85000000	50	0			13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-1						50.17000000			0	50	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-1						50.20000000			0	50	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-2						50.23000000			0	50	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-2						50.27000000			12	35	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-3						50.30000000			0	50	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-3						50.33000000			0	50	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-4						50.37000000			0	50	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-4						50.40000000			0	50	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-1						52.33000000			0	50	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-1						52.37000000			3	47	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-2						52.40000000			0	50	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-2						52.43000000			10	40	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-3						52.47000000			1	49	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-3						52.50000000			8	42	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-4						52.53000000			0	50	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-4						52.57000000			3	47	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-1						54.28000000			23	28	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-1						54.62000000			29	30	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-2						54.73000000			18	32	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-2						55.02000000			42	10	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-3						55.27000000			8	46	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-3						55.32000000			10	47	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-4						55.37000000			21	37	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-4						55.40000000			29	26	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-1						56.93000000			3	67	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-1						57.02000000			37	15	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-2						57.20000000			36	17	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-2						57.28000000			49	4	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-3						57.50000000			48	20	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-3						57.53000000			67	5	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-4						57.58000000			39	13	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-4						57.62000000			66	13	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-1						59.58000000			50	0	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-1						59.62000000			50	0	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-2						59.65000000			50	0	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-2						59.68000000			50	0	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-3						59.72000000			50	0	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-3						59.75000000			50	0	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-4						59.78000000			50	0	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-4						59.82000000			50	0	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-5						67.25000000			0	50	13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-5						67.33000000			2	25	13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-6						67.42000000			0	50	13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-6						67.50000000			0	50	13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-7						67.58000000			0	50	13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-7						67.67000000			0	50	13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-8						67.75000000			0	50	13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-8						67.83000000			0	50	13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-5						69.00000000			0	50	13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-5						69.00000000			0	20	13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-6						69.00000000			0	50	13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-6						69.08000000			0	50	13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-7						69.17000000			0	50	13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-7						69.25000000			0	50	13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-8						69.33000000			0	50	13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-8						69.42000000			50	0	13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-5						72.00000000			20	1	13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-5						72.00000000			50	0	13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-6						72.50000000			50	0	13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-6						72.50000000			50	0	13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-7						72.50000000			50	0	13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-7						72.50000000			50	0	13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	13	Low CO2	13-L-8						72.50000000			50	0	13.6	33.1	8.0	396.8	3.5	2.3	2225.2	8	17	443	441	1906	128	2052	1.97	3.09
Strongylocentrotus purpuratus (Echinoderm)	13	High CO2	13-H-8						72.50000000			50	0	13.6	33.1	7.6	1124.2	1.6	1.0	2225.2	8	48	1218	1213	2086	56	2190	0.86	1.35
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-9						53.67000000			0	50	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-9						53.78000000			0	50	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-9						52.87000000			0	50	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-9						52.98000000			0	50	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-10						52.47000000			5	45	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-10						52.53000000			1	49	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-11						51.75000000			18	32	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-11						51.68000000			33	17	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-9						55.17000000			0	50	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-9						55.22000000			0	50	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-10						54.28000000			0	50	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-10						54.38000000			0	50	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-11						53.63000000			19	31	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-11						53.67000000			25	25	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-12						52.72000000			32	18	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-12						52.77000000			47	3	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-9						57.93000000			5	45	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-9						58.00000000			0	50	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-10						57.05000000			0	50	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-10						57.10000000			0	50	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-11						58.27000000			45	5	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-11						58.37000000			54	0	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-12						57.40000000			44	6	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-12						57.43000000			54	0	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-10						57.83000000			50	0	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-10						57.83000000			50	0	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-11						56.83000000			50	0	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-11						56.83000000			50	0	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63
Strongylocentrotus purpuratus (Echinoderm)	18	High CO2	18-H-12						55.83000000			50	0	18.4	33.1	7.6	1181.3	1.8	1.2	2224.8	8	42	1243	1238	2061	66	2170	1.04	1.61
Strongylocentrotus purpuratus (Echinoderm)	18	Low CO2	18-L-12						55.83000000			50	0	18.4	33.1	8.0	398.1	4.1	2.7	2224.8	8	15	445	444	1854	150	2020	2.34	3.63