Citation:

j.1574-6941.2012.01443.x

Name: Effect of increased pCO2 on bacterial assemblage shifts in response to glucose addition in Fram Strait seawater mesocosms
Published: 2012
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Arctic; Community composition and diversity; Entire community; Field experiment; Gene expression (incl. proteomics); Mesocosm or benthocosm; Open ocean; Other metabolic rates; Pelagos; Polar

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Abstract:

Ocean acidification may stimulate primary production through increased availability of inorganic carbon in the photic zone, which may in turn change the biogenic flux of dissolved organic carbon (DOC) and the growth potential of heterotrophic bacteria. To investigate the effects of ocean acidification on marine bacterial assemblages, a two-by-three factorial mescosom experiment was conducted using surface sea water from the East Greenland Current in Fram Strait. Pyrosequencing of the V1-V2 region of bacterial 16S ribosomal RNA genes was used to investigate differences in the endpoint (Day 9) composition of bacterial assemblages in mineral nutrient-replete mesocosms amended with glucose (0 µm, 5.3 µm and 15.9 µm) under ambient (250 µatm) or acidified (400 µatm) partial pressures of CO2 (pCO2). All mesocosms showed low richness and diversity by Chao1 estimator and Shannon index, respectively, with general dominance by Gammaproteobacteria and Flavobacteria. Nonmetric multidimensional scaling analysis and two-way analysis of variance of the Jaccard dissimilarity matrix (97% similarity cut-off) demonstrated that the significant community shift between 0 µm and 15.9 µm glucose addition at 250 µatm pCO2 was eliminated at 400 µatm pCO2. These results suggest that the response potential of marine bacteria to DOC input may be altered under acidified conditions.

Keyword(s):

Arctic; Community composition and diversity; Entire community; Field experiment; Gene expression (incl. proteomics); Mesocosm or benthocosm; Open ocean; Other metabolic rates; Pelagos; Polar

Further details:

Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 76.930000 * Longitude: -3.580000

Date/Time Start: 2009-06-01T00:00:00 * Date/Time End: 2009-06-30T00:00:00

Event(s):

Fram_Strait_OA * Latitude: 76.930000 * Longitude: -3.580000 * Date/Time Start: 2009-06-01T00:00:00 * Date/Time End: 2009-06-30T00:00:00 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-03-24.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Ray, Jessica L
2	Incubation duration	Inc dur	days	Ray, Jessica L
3	Treatment	Treat		Ray, Jessica L		F=future pCO2, P=present pCO2, 0=no glucose, 1=5.3 µM glucose, 3=15.9 µM glucose
4	Bacteria, abundance	Bact abund	10³ #/cm³	Ray, Jessica L
5	Algae abundance	Algae ab	#/ml	Ray, Jessica L
6	Class	Class		Ray, Jessica L
7	Sequence coverage	Seq cov	%	Ray, Jessica L		on class level
8	Family	Family		Ray, Jessica L
9	Sequence coverage	Seq cov	%	Ray, Jessica L		on family level
10	Operational taxonomic unit	OTU	#	Ray, Jessica L
11	Sequence abundance	sequence abund	#	Ray, Jessica L
12	Bacterial production	BP	µg/l	Ray, Jessica L
13	Salinity	Sal		Ray, Jessica L
14	Temperature, water	Temp	°C	Ray, Jessica L
15	Alkalinity, total	AT	µmol/kg	Bellerby, Richard G J
16	Carbon, inorganic, dissolved	DIC	µmol/kg	Silyakova, Anna
17	Phosphate	PHSPHT	µmol/kg	Ray, Jessica L
18	Silicate	SILCAT	µmol/kg	Ray, Jessica L
19	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
20	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
21	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
22	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
23	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
24	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
25	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
26	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
27	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

112442 data points

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