Citation:

s00227-012-2059-6

Name: Seawater carbonate chemistry, the abiotic conditions in the fluid surrounding the embryo, growth, calcification of the cuttlefish Sepia officinalis in a laboratory experiment
Published: 2013
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Growth/Morphology; Laboratory experiment; Mediterranean Sea; Mollusca; Nekton; Pelagos; Reproduction; Sepia officinalis; Single species; Temperate; Temperature

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Abstract:

This study investigated the effects of seawater pH (i.e., 8.10, 7.85 and 7.60) and temperature (16 and 19 °C) on (a) the abiotic conditions in the fluid surrounding the embryo (viz. the perivitelline fluid), (b) growth, development and (c) cuttlebone calcification of embryonic and juvenile stages of the cephalopod Sepia officinalis. Egg swelling increased in response to acidification or warming, leading to an increase in egg surface while the interactive effects suggested a limited plasticity of the swelling modulation. Embryos experienced elevated pCO2 conditions in the perivitelline fluid (>3-fold higher pCO2 than that of ambient seawater), rendering the medium under-saturated even under ambient conditions. The growth of both embryos and juveniles was unaffected by pH, whereas 45Ca incorporation in cuttlebone increased significantly with decreasing pH at both temperatures. This phenomenon of hypercalcification is limited to only a number of animals but does not guarantee functional performance and calls for better mechanistic understanding of calcification processes.

Keyword(s):

Animalia; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Growth/Morphology; Laboratory experiment; Mediterranean Sea; Mollusca; Nekton; Pelagos; Reproduction; Sepia officinalis; Single species; Temperate; Temperature

Further details:

Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-03-18.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Dorey, Narimane
2	Experiment	Exp		Dorey, Narimane
3	Treatment	Treat		Dorey, Narimane
4	Alkalinity, total	AT	µmol/kg	Dorey, Narimane	Potentiometric titration
5	Alkalinity, total, standard deviation	AT std dev	±	Dorey, Narimane	Potentiometric titration
6	Salinity	Sal		Dorey, Narimane
7	Temperature, water	Temp	°C	Dorey, Narimane
8	Temperature, water, standard deviation	Temp std dev	±	Dorey, Narimane
9	pH	pH		Dorey, Narimane	Potentiometric	total scale
10	pH, standard deviation	pH std dev	±	Dorey, Narimane	Potentiometric	total scale
11	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Dorey, Narimane	Calculated using seacarb
12	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Dorey, Narimane	Calculated using seacarb
13	Aragonite saturation state	Omega Arg		Dorey, Narimane	Calculated using seacarb
14	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Dorey, Narimane	Calculated using seacarb
15	Oxygen, partial pressure, perivitelline fluid	PVF pO2	%	Dorey, Narimane		% air saturation
16	Oxygen, partial pressure, perivitelline fluid, standard deviation	PVF pO2 std dev	±	Dorey, Narimane		% air saturation
17	pH, perivitelline fluid	PVF pH		Dorey, Narimane		seawater scale
18	pH, perivitelline fluid, standard deviation	PVF pH std dev	±	Dorey, Narimane		seawater scale
19	Partial pressure of carbon dioxide, perivitelline fluid	PVF pCO2	µatm	Dorey, Narimane
20	Partial pressure of carbon dioxide, perivitelline fluid, standard deviation	PVF pCO2 std dev	±	Dorey, Narimane
21	Aragonite saturation state, perivitelline fluid	PVF Omega Arg		Dorey, Narimane
22	Aragonite saturation state, perivitelline fluid, standard deviation	PVF Omega Arg std dev	±	Dorey, Narimane
23	Eggs weight, fresh	Egg Wf	g	Dorey, Narimane
24	Eggs weight, fresh, standard deviation	Egg Wf std dev	±	Dorey, Narimane
25	Body mass, dry	BM dry	mg	Dorey, Narimane		embryo
26	Body mass, dry, standard deviation	BM dry std dev	±	Dorey, Narimane		embryo
27	Cuttlebone weight, dry	Cb DW	mg	Dorey, Narimane		embryo
28	Cuttlebone weight, dry, standard deviation	Cb DW std dev	±	Dorey, Narimane		embryo
29	Body mass, dry	BM dry	mg	Dorey, Narimane		juveniles
30	Body mass, dry, standard deviation	BM dry std dev	±	Dorey, Narimane		juveniles
31	Cuttlebone weight, dry	Cb DW	mg	Dorey, Narimane		juveniles
32	Cuttlebone weight, dry, standard deviation	Cb DW std dev	±	Dorey, Narimane		juveniles
33	Calcium-45 content per skeleton element	Qca	µmol/#	Dorey, Narimane		embryo, per cuttlebone
34	Calcium-45 content per skeleton element, standard deviation	Qca std dev	±	Dorey, Narimane		embryo, per cuttlebone
35	Calcium-45 content per skeleton element	Qca	µmol/#	Dorey, Narimane		juveniles, per cuttlebone
36	Calcium-45 content per skeleton element, standard deviation	Qca std dev	±	Dorey, Narimane		juveniles, per cuttlebone
37	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

420 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 Exp	3 Treat	4 AT [µmol/kg] (Potentiometric titration)	5 AT std dev [±] (Potentiometric titration)	6 Sal	7 Temp [°C]	8 Temp std dev [±]	9 pH (total scale, Potentiometric)	10 pH std dev [±] (total scale, Potentiometric)	11 pCO2water_SST_wet [µatm] (Calculated using seacarb)	12 pCO2 std dev [±] (Calculated using seacarb)	13 Omega Arg (Calculated using seacarb)	14 Omega Arg std dev [±] (Calculated using seacarb)	15 PVF pO2 [%] (% air saturation)	16 PVF pO2 std dev [±] (% air saturation)	17 PVF pH (seawater scale)	18 PVF pH std dev [±] (seawater scale)	19 PVF pCO2 [µatm]	20 PVF pCO2 std dev [±]	21 PVF Omega Arg	22 PVF Omega Arg std dev [±]	23 Egg Wf [g]	24 Egg Wf std dev [±]	25 BM dry [mg] (embryo)	26 BM dry std dev [±] (embryo)	27 Cb DW [mg] (embryo)	28 Cb DW std dev [±] (embryo)	29 BM dry [mg] (juveniles)	30 BM dry std dev [±] (juveniles)	31 Cb DW [mg] (juveniles)	32 Cb DW std dev [±] (juveniles)	33 Qca [µmol/#] (embryo, per cuttlebone)	34 Qca std dev [±] (embryo, per cuttlebone)	35 Qca [µmol/#] (juveniles, per cuttlebone)	36 Qca std dev [±] (juveniles, per cuttlebone)	37 CSC flag (Calculated using seacarb afte...)	38 CO2 [µmol/kg] (Calculated using seacarb afte...)	39 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	40 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	41 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	42 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	43 DIC [µmol/kg] (Calculated using seacarb afte...)	44 Omega Arg (Calculated using seacarb afte...)	45 Omega Cal (Calculated using seacarb afte...)
Sepia officinalis (mollusk)	1 (2008)	Temp 16-pH 7.6	2597	12	38	16.0	0.1	7.60	0.08	1433	208	1.21	0.31									1.84	0.10	32.200	2.100	1.400	0.200					20.600	1.300			8	50	1411	1406	2404	79	2533	1.19	1.84
Sepia officinalis (mollusk)	1 (2008)	Temp 16-pH 7.85	2597	12	38	16.0	0.1	7.84	0.05	775	83	1.97	0.20									1.79	0.07	31.700	0.600	1.700	0.200					18.200	0.600			8	27	770	767	2279	130	2436	1.96	3.03
Sepia officinalis (mollusk)	1 (2008)	Temp 16-pH 8.1	2597	12	38	16.0	0.1	8.11	0.09	378	65	3.35	0.63									1.53	0.03	33.300	0.600	1.200	0.200					17.600	1.800			8	13	374	373	2061	219	2294	3.30	5.11
Sepia officinalis (mollusk)	1 (2008)	Temp 19-pH 7.6	2597	12	38	18.9	0.3	7.58	0.08	1517	273	1.30	0.26									2.34	0.08	25.300	1.900	3.300	1.000					13.600	0.700			8	49	1499	1494	2393	84	2526	1.27	1.96
Sepia officinalis (mollusk)	1 (2008)	Temp 19-pH 7.85	2597	12	38	18.9	0.3	7.86	0.12	766	181	2.32	0.73									2.23	0.09	26.900	1.300	3.400	2.000					13.200	0.500			8	24	734	732	2234	149	2407	2.26	3.48
Sepia officinalis (mollusk)	1 (2008)	Temp 19-pH 8.1	2597	12	38	18.9	0.3	8.14	0.09	393	83	3.56	0.67									2.28	0.10	26.800	1.500	3.000	0.500					11.800	0.800			8	11	342	341	1982	252	2245	3.83	5.88
Sepia officinalis (mollusk)	2 (2009)	Temp 16-pH 7.6	2549	65	38	16.0	0.4	7.60	0.09	1381	30	1.16	0.03	23.4	2.2	7.07	0.00942809	4354	177.7596	0.34	0.01080123	3.14	0.21					43.9	5.9	3.2	0.5			4.3	1.1	8	49	1385	1380	2359	77	2486	1.17	1.81
Sepia officinalis (mollusk)	2 (2009)	Temp 16-pH 7.85	2549	65	38	16.0	0.4	7.81	0.04	739	15	1.97	0.04	25.4	3.1	7.21	0.01646545	3074	192.5744	0.44	0.01567021	2.75	0.18					44.3	3.0	3.1	0.3			2.3	0.4	8	29	816	813	2254	120	2404	1.81	2.80
Sepia officinalis (mollusk)	2 (2009)	Temp 16-pH 8.1	2549	65	38	16.0	0.4	8.06	0.08	376	11	3.17	0.09	27.1	2.4	7.35	0.02529822	2311	142.7301	0.64	0.03777124	3.01	0.16					42.7	4.6	2.7	0.4			2.7	0.6	8	15	421	420	2068	196	2279	2.95	4.57
Sepia officinalis (mollusk)	2 (2009)	Temp 19-pH 7.6	2549	65	38	19.1	0.5	7.60	0.03	1395	38	1.30	0.04	24.1	2.3	7.10	0.02850439	4238	249.4897	0.42	0.03150964	3.80	0.41					40.8	10.0	3.3	1.1			3.6	1.1	8	46	1400	1395	2338	86	2470	1.31	2.02
Sepia officinalis (mollusk)	2 (2009)	Temp 19-pH 7.85	2549	65	38	19.1	0.5	7.83	0.02	741	22	2.19	0.06	24.2	2.0	7.22	0.02988868	3020	230.5835	0.53	0.05981453	3.72	0.17					40.5	0.6	3.9	0.7			2.7	0.3	8	25	779	777	2210	139	2374	2.11	3.24
Sepia officinalis (mollusk)	2 (2009)	Temp 19-pH 8.1	2549	65	38	19.1	0.5	8.03	0.06	370	7	3.46	0.06	22.9	3.0	7.45	0.02926369	1822	163.6248	0.89	0.03931227	3.56	0.31					38.9	1.7	3.8	0.8			2.0	0.5	8	15	456	455	2050	204	2269	3.10	4.76