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Hofmann, Laurie C; Straub, Susanne M; Bischof, Kai (2014): Experiment: Elevated CO2 levels affect the activity of nitrate reductase and carbonic anhydrase in the calcifying rhodophyte Corallina officinalis. PANGAEA, https://doi.org/10.1594/PANGAEA.830299, Supplement to: Hofmann, LC et al. (2013): Elevated CO2 levels affect the activity of nitrate reductase and carbonic anhydrase in the calcifying rhodophyte Corallina officinalis. Journal of Experimental Botany, 64(4), 899-908, https://doi.org/10.1093/jxb/ers369

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Abstract:
The concentration of CO2 in global surface ocean waters is increasing due to rising atmospheric CO2 emissions, resulting in lower pH and a lower saturation state of carbonate ions. Such changes in seawater chemistry are expected to impact calcification in calcifying marine organisms. However, other physiological processes related to calcification might also be affected, including enzyme activity. In a mesocosm experiment, macroalgal communities were exposed to three CO2 concentrations (380, 665, and 1486 µatm) to determine how the activity of two enzymes related to inorganic carbon uptake and nutrient assimilation in Corallina officinalis, an abundant calcifying rhodophyte, will be affected by elevated CO2 concentrations. The activity of external carbonic anhydrase, an important enzyme functioning in macroalgal carbon-concentrating mechanisms, was inversely related to CO2 concentration after long-term exposure (12 weeks). Nitrate reductase, the enzyme responsible for reduction of nitrate to nitrite, was stimulated by CO2 and was highest in algae grown at 665 µatm CO2. Nitrate and phosphate uptake rates were inversely related to CO2, while ammonium uptake was unaffected, and the percentage of inorganic carbon in the algal skeleton decreased with increasing CO2. The results indicate that the processes of inorganic carbon and nutrient uptake and assimilation are affected by elevated CO2 due to changes in enzyme activity, which change the energy balance and physiological status of C. officinalis, therefore affecting its competitive interactions with other macroalgae. The ecological implications of the physiological changes in C. officinalis in response to elevated CO2 are discussed.
Keyword(s):
Benthos; Coast and continental shelf; Containers and aquaria (20- 1000 L or < 1 m**2); Corallina officinalis; Laboratory experiment; Macroalgae; North Atlantic; Other metabolic rates; Plantae; Rhodophyta; Single species; Temperate
Related to:
Hofmann, Laurie C (2014): Experiment: Elevated CO2 levels affect the activity of nitrate reductase and carbonic anhydrase in the calcifying rhodophyte Corallina officinalis (Fig. 2: Nutrients). PANGAEA, https://doi.org/10.1594/PANGAEA.830300
Hofmann, Laurie C (2014): Experiment: Elevated CO2 levels affect the activity of nitrate reductase and carbonic anhydrase in the calcifying rhodophyte Corallina officinalis (Fig.3: Temperature and pH). PANGAEA, https://doi.org/10.1594/PANGAEA.830301
Further details:
Lavigne, Héloise; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-02-11.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1SpeciesSpeciesHofmann, Laurie C
2IdentificationIDHofmann, Laurie C
3TreatmentTreatHofmann, Laurie C
4Phosphate uptake ratePO4 upt rateµmol/m2/hHofmann, Laurie C
5Nitrate uptake rateNO3 upt rateµmol/m2/hHofmann, Laurie C
6Ammonium uptake rate[NH4]+ upt rateµmol/m2/dayHofmann, Laurie C
7DateDateHofmann, Laurie C
8GroupGroupHofmann, Laurie C
9Carbonic anhydrase, activityCA activity1/gHofmann, Laurie CTotal CA activity
10Carbonic anhydrase, activityCA activity1/gHofmann, Laurie CExternal CA activity
11Carbon, inorganic, totalTIC%Hofmann, Laurie C
12Nitrate reductase activityNO3 reduct actµmol/g/hHofmann, Laurie C
13SalinitySalHofmann, Laurie C
14Temperature, waterTemp°CHofmann, Laurie C
15Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmHofmann, Laurie C
16Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Hofmann, Laurie C
17pHpHHofmann, Laurie CNBS scale
18pH, standard deviationpH std dev±Hofmann, Laurie CNBS scale
19Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
20pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
21Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
22Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
23Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
24Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
25Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
26Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
27Alkalinity, totalATµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
28Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
29Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Size:
16955 data points

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