Citation:

j.1529-8817.2010.00916.x

Always quote citation above when using data! You can download the citation in several formats below.

RIS Citation BibTeX Citation Text Citation Facebook Twitter

Abstract:

The coccolithophore Emiliania huxleyi (Lohmann) W. W. Hay et H. Mohler was cultured in natural seawater with the addition of either the microtubule-inhibitor colchicine, the actin-inhibitor cytochalasin B, or the photosynthesis inhibitor 3-(3,4 dichlorophenyl)-1,1-dimethyl-urea (DCMU). Additionally, E. huxleyi was cultured at different light intensities and temperatures. Growth rate was monitored, and coccolith morphology analyzed. While every treatment affected growth rate, the percentage of malformed coccoliths increased with colchicine, cytochalasin B, and at higher than optimal temperature. These results represent the first experimental evidence for the role of microtubules and actin microfilaments in coccolith morphogenesis.

Project(s):

Biological Impacts of Ocean Acidification (BIOACID)

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator
1	Variable	Variable		Langer, Gerald
2	Treatment	Treat		Langer, Gerald
3	Growth rate	µ	#/day	Langer, Gerald
4	Growth rate, standard deviation	µ std dev	±	Langer, Gerald
5	Coccoliths, normal	Cocco norm	%	Langer, Gerald
6	Coccoliths, normal, standard deviation	Cocco norm std dev	±	Langer, Gerald
7	Malformation rate	Malformation	%	Langer, Gerald
8	Malformation rate, standard deviation	Malformation std dev	±	Langer, Gerald
9	Coccoliths, incomplete	Cocco incomplete	%	Langer, Gerald
10	Coccoliths, incomplete, standard deviation	Cocco incomplete std dev	±	Langer, Gerald
11	Coccoliths, malformed and incomplete	Cocco malformed	%	Langer, Gerald
12	Coccoliths, malformed and incomplete, standard deviation	Cocco malformed std dev	±	Langer, Gerald

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Size:

204 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Variable	2 Treat	3 µ [#/day]	4 µ std dev [±]	5 Cocco norm [%]	6 Cocco norm std dev [±]	7 Malformation [%]	8 Malformation std dev [±]	9 Cocco incomplete [%]	10 Cocco incomplete std dev [±]	11 Cocco malformed [%]	12 Cocco malformed std dev [±]
Colchicine III	Control	1.35	0.01	91.90	0.17	3.27	1.02	1.93	0.47	2.90	0.71
Colchicine III	500 µM	1.25	0.01	89.67	2.15	4.93	0.01	1.78	0.42	3.62	2.56
Colchicine IV	Control	1.34	0.01	92.45	0.87	4.00	0.76	1.15	0.34	2.40	0.45
Colchicine IV	750 µM	0.95	0.00	81.27	1.66	8.93	2.85	2.47	1.03	7.33	0.16
Colchicine II	Control	1.33	0.02	90.85	1.88	4.75	0.69	2.38	0.34	2.02	0.85
Colchicine II	1000 µM	0.59	0.04	42.25	4.10	22.09	1.54	2.53	0.14	33.14	2.69
Cytochalasin test	0.1 µM	1.15
Cytochalasin test	1 µM	1.18
Cytochalasin test	10 µM	1.02
Cytochalasin test	100 µM	0.20
Cytochalasin	Control	1.51	0.03	86.99	3.96	6.33	3.38	2.42	1.50	4.25	2.08
Cytochalasin	100 µM	0.20	0.02	15.31	1.10	29.34	2.82	2.55	1.71	52.80	3.43
Photosynthesis	Control	1.41	0.03	88.91	2.12	4.21	1.11	3.27	0.75	3.61	0.27
Photosynthesis	DCMU, 0.01 µM	0.58	0.01	92.82	1.55	3.49	0.08	3.07	1.35	0.62	0.13
Photosynthesis	Low light, 5-7 µMol photons/m**2/s	0.56	0.08	87.67	4.61	7.18	3.91	4.28	0.79	0.87	0.09
DMSO	Control	1.23	0.15
DMSO	0.04vol %	1.41	0.01
DMSO	0.1vol %	1.44	0.03
DMSO	0.2vol %	1.41	0.02
DMSO	0.5vol %	1.41	0.00
Temperature	10?C			80.99	10.77	7.99	1.77	1.86	0.44	9.16	8.56
Temperature	15?C			86.87	0.27	3.39	0.53	3.43	1.19	6.31	0.39
Temperature	20?C			89.08	1.23	4.05	0.87	3.17	0.37	3.69	0.01
Temperature	25?C			28.86	1.33	24.67	2.58	1.73	1.27	44.74	2.52