/* DATA DESCRIPTION: Citation: Bach, Lennart Thomas (2014): Experiment: changing carbonate chemistry influence on coccoliths formed by Emiliania huxleyi. PANGAEA, https://doi.org/10.1594/PANGAEA.829081, Supplement to: Bach, Lennart Thomas; Bauke, Christine; Meier, K J Sebastian; Riebesell, Ulf; Schulz, Kai Georg (2012): Influence of changing carbonate chemistry on morphology and weight of coccoliths formed by Emiliania huxleyi. Biogeosciences, 9(8), 3449-3463, https://doi.org/10.5194/bg-9-3449-2012 Abstract: The coccolithophore Emiliania huxleyi is a marine phytoplankton species capable of forming small calcium carbonate scales (coccoliths) which cover the organic part of the cell. Calcification rates of E. huxleyi are known to be sensitive to changes in seawater carbonate chemistry. It has, however, not yet been clearly determined how these changes are reflected in size and weight of individual coccoliths and which specific parameter(s) of the carbonate system drive morphological modifications. Here, we compare data on coccolith size, weight, and malformation from a set of five experiments with a large diversity of carbonate chemistry conditions. This diversity allows distinguishing the influence of individual carbonate chemistry parameters such as carbon dioxide (CO2), bicarbonate (HCO3- ), carbonate ion (CO32-), and protons (H+) on the measured parameters. Measurements of fine-scale morphological structures reveal an increase of coccolith malformation with decreasing pH suggesting that H+ is the major factor causing malformations. Coccolith distal shield area varies from about 5 to 11 µm2. Changes in size seem to be mainly induced by varying [HCO3- ] and [H+] although influence of [CO32-] cannot be entirely ruled out. Changes in coccolith weight were proportional to changes in size. Increasing CaCO3 production rates are reflected in an increase in coccolith weight and an increase of the number of coccoliths formed per unit time. The combined investigation of morphological features and coccolith production rates presented in this study may help to interpret data derived from sediment cores, where coccolith morphology is used to reconstruct calcification rates in the water column. Project(s): Biological Impacts of Ocean Acidification (BIOACID) (URI: https://www.bioacid.de/) Parameter(s): Treatment (Treat) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) Carbon, inorganic, dissolved [µmol/kg] (DIC) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) Alkalinity, total [µmol/kg] (AT) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) pH (pH) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) Carbon dioxide, partial pressure [µatm] (pCO2) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) Bicarbonate ion [µmol/kg] ([HCO3]-) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) Carbonate ion [µmol/kg] ([CO3]2-) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) Carbon dioxide [µmol/kg] (CO2) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) Calcite saturation state (Omega Cal) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) Aragonite saturation state (Omega Arg) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) Cell size [µm] (Cell size) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) Particulate inorganic carbon production per cell [pg/#/day] (PIC prod/cell) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) Mass [µg] (Mass) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) * COMMENT: mean coccolith weight Mass, standard deviation [±] (Mass std dev) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) * COMMENT: coccolith weight stdev - (-) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) * COMMENT: CaCO3 prod/cell Production [1/day] (P) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) * COMMENT: number of coccoliths produced per day Surface area [µm**2] (SA) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) * COMMENT: coccolith distal shield area mean Surface area, standard deviation [±] (SA std dev) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) Length [µm] (l) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) * COMMENT: mean coccolith length Length, standard deviation [±] (l std dev) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) Width [µm] (w) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) * COMMENT: mean coccolith width Width, standard deviation [±] (w std dev) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) * METHOD/DEVICE: Measured Length [µm] (l) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) * COMMENT: mean central area length Length, standard deviation [±] (l std dev) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) Width [µm] (w) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) * COMMENT: mean central area width Width, standard deviation [±] (w std dev) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) * METHOD/DEVICE: Measured Height/width ratio (Height/width) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) * COMMENT: mean coccolith length/coccolith width ratio Height/width ratio, standard deviation [±] (Height/width std dev) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) Height/width ratio (Height/width) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) * COMMENT: mean central area length/central area width ratio Height/width ratio, standard deviation [±] (Height/width std dev) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) Surface area [µm**2] (SA) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) * COMMENT: mean calculated distal shield surface area Surface area, standard deviation [±] (SA std dev) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) Surface area [µm**2] (SA) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) * COMMENT: mean calculated central area surface area Surface area, standard deviation [±] (SA std dev) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) Coccoliths, malformed and incomplete [%] (Cocco malformed) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) * COMMENT: mean malformation Coccoliths, malformed and incomplete, standard deviation [±] (Cocco malformed std dev) * PI: Bach, Lennart Thomas (https://orcid.org/0000-0003-0202-3671, lennart.bach@utas.edu.au) License: Creative Commons Attribution 3.0 Unported (CC-BY-3.0) (URI: https://creativecommons.org/licenses/by/3.0/) Size: 1977 data points */ Treat DIC [µmol/kg] AT [µmol/kg] pH pCO2 [µatm] [HCO3]- [µmol/kg] [CO3]2- [µmol/kg] CO2 [µmol/kg] Omega Cal Omega Arg Cell size [µm] PIC prod/cell [pg/#/day] Mass [µg] (mean coccolith weight) Mass std dev [±] (coccolith weight stdev) - (CaCO3 prod/cell) P [1/day] (number of coccoliths produced...) SA [µm**2] (coccolith distal shield area ...) SA std dev [±] l [µm] (mean coccolith length) l std dev [±] w [µm] (mean coccolith width) w std dev [±] l [µm] (mean central area length) l std dev [±] w [µm] (mean central area width) w std dev [±] Height/width (mean coccolith length/coccoli...) Height/width std dev [±] Height/width (mean central area length/cent...) Height/width std dev [±] SA [µm**2] (mean calculated distal shield...) SA std dev [±] SA [µm**2] (mean calculated central area ...) SA std dev [±] Cocco malformed [%] (mean malformation) Cocco malformed std dev [±] constant_CO2 545.123 563.714 7.513 450.979 516.963 11.267 16.893 0.269 0.172 3.985 0.153 1.275381414 constant_CO2 534.833 560.688 7.577 382.348 507.693 12.817 14.322 0.306 0.196 3.979 0.296 2.466730629 constant_CO2 520.042 553.244 7.641 320.910 493.587 14.434 12.021 0.344 0.221 4.051 0.560 4.662706805 4.594 0.983 2.723 0.285 2.148 0.263 1.717 0.163 1.132 0.142 1.273 0.083 1.528 0.129 4.642 1.023 1.540 0.322 constant_CO2 822.211 860.779 7.664 481.343 780.140 24.041 18.031 0.573 0.368 4.653 4.026 1.070 0.600 33.54834918 7.489 1.732 3.399 0.383 2.773 0.371 1.707 0.177 1.110 0.167 1.231 0.060 1.551 0.119 7.508 1.749 1.507 0.359 0.458 0.152 constant_CO2 827.898 867.143 7.667 480.755 785.488 24.401 18.009 0.582 0.374 4.636 3.979 33.15351835 constant_CO2 829.317 867.143 7.658 492.443 786.960 23.911 18.447 0.570 0.366 4.607 4.387 36.55603398 34.167 constant_CO2 1159.280 1235.655 7.825 464.638 1092.990 48.885 17.405 1.165 0.748 4.620 6.275 1.646 1.422 52.29068289 31.775 8.318 1.755 3.543 0.363 2.952 0.356 1.610 0.193 1.035 0.162 1.204 0.061 1.566 0.112 8.301 1.782 1.329 0.351 0.344 0.193 constant_CO2 1183.500 1260.510 7.825 474.346 1115.826 49.906 17.769 1.190 0.764 4.707 6.418 2.415 1.564 53.48241115 22.149 constant_CO2 1180.578 1259.576 7.834 463.483 1112.449 50.767 17.362 1.210 0.777 4.692 6.715 1.345 1.073 55.95501388 41.590 constant_CO2 1912.840 2115.871 8.089 406.681 1753.805 143.802 15.234 3.428 2.201 4.688 8.099 1.838 1.200 67.48532978 36.722 9.614 1.780 3.787 0.360 3.205 0.330 1.692 0.231 1.112 0.159 1.183 0.047 1.525 0.067 9.618 1.850 1.504 0.415 0.295 0.119 constant_CO2 1909.666 2113.272 8.090 404.264 1750.419 144.103 15.143 3.435 2.206 4.710 7.992 1.784 1.383 66.59359251 37.336 constant_CO2 1912.179 2115.392 8.089 406.540 1753.199 143.752 15.229 3.426 2.200 4.674 7.628 1.736 0.872 63.56222723 36.620 constant_CO2 4117.424 4797.800 8.383 415.414 3531.162 570.701 15.561 13.603 8.736 4.668 9.304 1.846 1.095 77.52890959 42.003 10.622 2.269 3.952 0.414 3.377 0.410 1.699 0.165 1.112 0.144 1.173 0.047 1.537 0.104 10.607 2.367 1.499 0.320 0.288 0.099 constant_CO2 4107.309 4795.550 8.390 406.881 3514.788 577.279 15.241 13.760 8.837 4.669 9.251 2.087 1.441 77.0889015 36.937 constant_CO2 4111.680 4799.300 8.390 408.247 3519.497 576.890 15.293 13.750 8.831 4.604 10.230 1.906 1.469 85.24513784 44.730 constant_TA 1420.799 2334.102 9.060 19.913 803.539 616.514 0.746 14.695 9.437 3.566 2.366 0.903 0.924 19.71933749 5.936 1.066 3.031 0.283 2.453 0.251 1.331 0.170 0.796 0.124 1.238 0.051 1.683 0.135 5.890 1.088 0.846 0.229 0.255 0.093 constant_TA 1612.030 2324.410 8.810 49.614 1125.076 485.095 1.858 11.562 7.426 4.109 4.085 1.359 1.510 34.04349334 6.775 1.283 3.221 0.301 2.653 0.283 1.464 0.166 0.889 0.150 1.216 0.044 1.665 0.148 6.776 1.314 1.040 0.283 0.240 0.072 constant_TA 1748.580 2318.520 8.634 89.457 1354.966 390.262 3.351 9.302 5.974 4.253 5.403 1.662 1.879 45.02644529 8.925 1.859 3.642 0.377 3.073 0.358 1.495 0.224 0.918 0.178 1.189 0.062 1.645 0.129 8.881 1.888 1.106 0.375 0.239 0.065 constant_TA 1854.272 2315.272 8.492 140.314 1531.271 317.745 5.256 7.574 4.864 4.654 7.198 1.799 1.984 59.98389255 33.347 constant_TA 1941.618 2309.835 8.359 209.021 1677.637 256.151 7.830 6.105 3.921 4.774 8.939 1.944 2.218 74.4875857 38.323 constant_TA 1995.942 2292.541 8.245 287.899 1776.409 208.749 10.784 4.976 3.195 4.841 9.088 2.073 2.494 75.73140122 36.535 9.317 1.996 3.730 0.371 3.150 0.369 1.680 0.194 1.079 0.181 1.188 0.052 1.574 0.131 9.326 2.000 1.447 0.392 0.268 0.082 constant_TA 2063.838 2297.626 8.128 398.461 1880.025 168.886 14.926 4.025 2.585 4.841 11.001 1.927 2.229 91.67159636 47.564 constant_TA 2104.845 2302.031 8.052 491.995 1940.139 146.276 18.430 3.487 2.239 4.811 8.896 1.855 2.512 74.1312276 39.955 constant_TA 2131.437 2288.815 7.962 621.440 1986.125 122.034 23.279 2.909 1.868 4.841 7.230 1.736 1.987 60.24926151 34.698 9.930 2.471 3.855 0.441 3.256 0.453 1.634 0.230 1.064 0.175 1.189 0.054 1.546 0.131 10.005 2.514 1.393 0.414 0.367 0.160 constant_TA 2174.968 2304.775 7.893 744.669 2040.496 106.578 27.895 2.540 1.631 4.778 4.753 1.669 1.811 39.6027568 23.732 constant_TA 2192.344 2294.831 7.821 890.564 2067.521 91.464 33.360 2.180 1.400 4.673 6.026 1.580 1.930 50.21723629 31.775 constant_TA 2226.860 2314.290 7.777 1003.526 2105.040 84.229 37.591 2.008 1.289 4.501 7.072 1.604 1.821 58.93226145 36.751 9.745 2.063 3.841 0.399 3.210 0.374 1.701 0.192 1.133 0.152 1.199 0.045 1.511 0.131 9.791 2.100 1.529 0.362 0.459 0.095 constant_TA 2236.059 2302.484 7.716 1164.552 2118.568 73.868 43.623 1.761 1.131 4.843 5.977 1.489 1.711 49.80732062 33.446 constant_TA 2283.225 2291.483 7.537 1790.712 2166.256 49.890 67.079 1.189 0.764 4.704 4.111 1.252 1.283 34.25586943 27.366 constant_TA 2327.870 2294.101 7.410 2441.037 2198.595 37.836 91.439 0.902 0.579 4.587 3.513 1.277 1.538 29.27309712 22.927 8.004 1.632 3.496 0.358 2.886 0.342 1.615 0.171 1.053 0.147 1.215 0.071 1.546 0.139 8.008 1.686 1.350 0.311 0.487 0.138 constant_TA 2374.687 2306.940 7.316 3074.922 2228.619 30.884 115.184 0.736 0.473 4.552 2.951 1.281 1.379 24.58838012 19.199 7.254 1.552 3.315 0.350 2.763 0.338 1.626 0.159 1.078 0.111 1.204 0.076 1.515 0.147 7.272 1.592 1.384 0.246 0.439 0.117 constant_TA 2207.193 2296.316 7.781 984.812 2085.857 84.446 36.890 2.013 1.293 4.910 6.878 57.31449595 constant_TA 2418.182 2319.417 7.239 3728.994 2252.152 26.345 139.685 0.628 0.403 4.609 2.403 20.02501285 constant_TA 2459.458 2312.556 7.131 4787.293 2259.633 20.496 179.328 0.489 0.314 4.332 1.898 1.206 1.343 15.81718326 13.120 7.069 1.497 3.279 0.324 2.734 0.335 1.497 0.425 1.006 0.289 1.205 0.083 1.497 0.153 7.109 1.523 1.354 0.294 0.611 0.152 constant_TA 2513.604 2336.838 7.075 5493.916 2289.556 18.250 205.798 0.435 0.279 4.494 1.461 12.17094635 pH7_7 212.513 259.764 7.741 103.695 201.204 7.425 3.884 0.177 0.114 3.797 0.226 1.882085148 pH7_7 169.275 215.696 7.740 83.053 160.281 5.882 3.111 0.140 0.090 3.818 0.167 1.394773787 pH7_7 216.715 264.008 7.741 105.715 205.182 7.574 3.960 0.181 0.116 3.803 0.189 1.57568993 pH7_7 315.070 364.656 7.746 151.797 298.235 11.149 5.686 0.266 0.171 3.919 0.174 1.449758822 pH7_7 347.188 397.317 7.746 166.862 328.623 12.315 6.251 0.294 0.189 3.892 0.402 3.349293173 pH7_7 339.150 389.435 7.749 161.976 320.975 12.107 6.067 0.289 0.185 3.890 0.362 3.017180543 pH7_7 913.138 972.185 7.743 444.694 864.507 31.973 16.658 0.762 0.489 4.610 4.368 1.433 1.034 36.40090474 25.404 8.102 1.677 3.545 0.371 2.908 0.349 1.828 0.257 1.209 0.218 1.223 0.058 1.526 0.115 8.190 1.733 1.776 0.551 0.436 0.095 pH7_7 900.399 959.125 7.742 439.499 852.482 31.454 16.463 0.750 0.481 4.599 4.346 1.197 0.478 36.2161347 30.250 pH7_7 919.938 978.536 7.740 450.612 871.035 32.023 16.880 0.763 0.490 4.621 4.661 1.458 1.062 38.84029041 26.637 pH7_7 2027.149 2102.937 7.737 1002.255 1919.651 69.955 37.544 1.667 1.071 4.857 7.162 1.804 1.260 59.68014702 33.091 9.324 1.919 3.744 0.387 3.152 0.356 1.636 0.221 1.065 0.162 1.191 0.056 1.545 0.137 9.366 1.963 1.392 0.370 0.414 0.204 pH7_7 2027.796 2103.288 7.736 1004.488 1920.327 69.841 37.627 1.665 1.069 4.882 8.104 1.518 0.830 67.52961103 44.475 pH7_7 2023.438 2098.684 7.735 1004.305 1916.265 69.553 37.620 1.658 1.065 4.833 7.454 1.685 1.284 62.10961651 36.853 pH7_7 4071.232 4186.773 7.749 1955.943 3853.412 144.552 73.268 3.445 2.213 4.782 8.013 2.956 2.178 66.76869866 22.585 10.046 2.131 3.869 0.405 3.275 0.379 1.728 0.201 1.014 0.216 1.184 0.049 1.540 0.110 10.064 2.165 1.555 0.411 0.348 0.093 pH7_7 4048.331 4162.759 7.748 1949.132 3831.897 143.420 73.013 3.418 2.195 4.846 8.647 3.469 3.052 72.05429461 20.770 pH7_7 4057.676 4172.292 7.748 1953.462 3840.737 143.764 73.175 3.427 2.201 4.873 9.118 2.859 1.851 75.98099237 26.576 pH7_7 7376.234 7542.704 7.742 3601.076 6983.470 257.870 134.893 6.146 3.947 4.936 7.805 1.566 1.099 65.03530904 41.521 9.536 2.202 3.767 0.422 3.187 0.408 1.581 0.246 1.129 0.167 1.185 0.049 1.580 0.142 9.557 2.258 1.297 0.479 0.447 0.159 pH7_7 7363.601 7527.080 7.739 3616.326 6972.284 255.852 135.465 6.098 3.916 4.942 8.386 1.648 0.772 69.88104325 42.394 pH7_7 7355.635 7521.911 7.742 3591.413 6963.981 257.122 134.532 6.129 3.936 4.979 8.764 1.870 1.603 73.02663039 39.053 pH8 148.053 217.349 7.984 40.532 137.644 8.891 1.518 0.212 0.136 3.574 0.945 7.875573149 pH8 300.005 374.134 7.995 78.881 274.401 18.149 2.955 0.433 0.278 3.709 0.000 0 pH8 351.350 432.239 7.992 94.612 326.395 21.411 3.544 0.510 0.328 3.853 0.000 0 pH8 486.117 574.885 7.997 129.240 451.310 29.965 4.841 0.714 0.459 4.266 2.015 1.342 0.896 16.78797817 12.508 5.463 1.118 2.984 0.284 2.314 0.272 1.770 0.188 1.133 0.174 1.294 0.065 1.576 0.125 5.478 1.148 1.596 0.409 pH8 705.425 804.339 7.992 189.864 655.307 43.005 7.112 1.025 0.658 4.519 2.984 1.472 1.301 24.86837721 16.896 6.814 1.194 3.290 0.283 2.629 0.270 1.733 0.169 1.121 0.158 1.254 0.053 1.559 0.125 6.848 1.232 1.542 0.345 0.389 0.133 pH8 808.091 910.486 7.985 221.390 751.312 48.486 8.293 1.156 0.742 4.830 5.067 1.712 1.852 42.22615654 24.669 9.114 1.748 3.723 0.351 3.091 0.304 1.607 0.152 0.985 0.132 1.206 0.052 1.644 0.135 9.113 1.749 1.256 0.272 0.335 0.119 pH8 1430.177 1564.749 7.988 388.265 1329.124 86.508 14.544 2.062 1.324 4.681 6.182 2.131 1.535 51.513248 24.169 pH8 1817.436 1971.159 7.988 494.384 1689.180 109.737 18.519 2.616 1.680 4.668 6.800 2.055 1.814 56.66184745 27.573 10.758 2.246 3.947 0.498 3.378 0.477 1.667 0.200 1.026 0.172 1.177 0.094 1.641 0.138 10.646 2.527 1.367 0.384 0.302 0.120 pH8 2283.465 2464.784 7.996 608.396 2120.117 140.558 22.790 3.350 2.152 4.801 1.948 1.498 pH8 2879.129 3084.670 7.985 788.453 2676.726 172.868 29.535 4.120 2.646 4.860 2.227 1.152 10.767 2.447 3.991 0.420 3.393 0.417 1.743 0.205 1.145 0.191 1.179 0.040 1.537 0.122 10.765 2.450 1.595 0.438 0.286 0.061 pH8 3417.045 3656.191 7.995 912.015 3172.952 209.930 34.163 5.004 3.214 4.734 8.933 2.169 1.536 74.43774489 34.323 pH8 4024.885 4299.221 8.001 1060.717 3735.083 250.068 39.734 5.961 3.828 4.827 7.492 2.300 1.667 62.4344753 27.140 10.695 2.399 3.971 0.439 3.391 0.400 1.695 0.191 1.085 0.169 1.173 0.042 1.576 0.121 10.706 2.423 1.466 0.377 0.307 0.096 pH8 5249.381 5582.693 7.995 1401.150 4874.472 322.423 52.486 7.685 4.935 4.809 6.638 2.269 1.450 55.31270722 24.379 pH8 5623.630 5973.584 7.993 1510.813 5223.558 343.478 56.594 8.187 5.258 4.681 2.287 1.414 11.509 2.540 4.103 0.431 3.532 0.435 1.567 0.166 0.925 0.151 1.165 0.052 1.713 0.151 11.518 2.620 1.155 0.297 0.339 0.124 pH8 7359.831 7808.457 8.001 1939.629 6829.893 457.281 72.657 10.900 7.000 4.791 6.658 2.304 1.866 55.47729015 24.074 pH8 8994.166 9525.461 7.999 2380.435 8348.324 556.673 89.169 13.269 8.521 4.732 11.145 2.216 4.026 0.404 3.496 0.378 1.556 0.187 0.905 0.136 1.153 0.047 1.729 0.114 11.164 2.278 1.051 0.401 0.343 0.124 pH8 12360.488 13077.752 8.004 3230.252 11465.641 773.844 121.003 18.445 11.846 4.773 5.451 45.42336924 pH8 14084.584 14899.153 8.007 3657.234 13060.760 886.827 136.997 21.138 13.575 4.781 5.752 47.9309627 9.996 2.303 3.836 0.431 3.282 0.403 1.690 0.215 1.061 0.207 1.171 0.048 1.622 0.188 10.015 2.327 1.437 0.435 0.369 0.166 pH8_3 198.586 335.571 8.320 23.584 173.419 24.284 0.883 0.579 0.372 3.379 0.060 0.503060518 pH8_3 178.597 316.091 8.332 20.530 155.447 22.381 0.769 0.533 0.343 3.402 0.083 0.693169614 pH8_3 193.529 332.784 8.332 22.274 168.465 24.230 0.834 0.578 0.371 3.374 0.095 0.794116723 pH8_3 893.814 1117.474 8.332 102.918 778.095 111.864 3.855 2.666 1.712 4.428 4.468 1.568 0.402 37.22962784 23.746 6.423 1.166 3.177 0.283 2.561 0.260 1.613 0.188 1.022 0.164 1.243 0.045 1.590 0.107 6.442 1.196 1.317 0.364 0.365 0.105 pH8_3 898.802 1125.328 8.338 102.142 781.337 113.639 3.826 2.709 1.740 4.326 4.426 2.077 1.219 36.87843667 17.755 pH8_3 897.938 1123.934 8.337 102.267 780.771 113.336 3.831 2.701 1.735 4.320 4.337 1.720 0.592 36.1434215 21.009 pH8_3 2247.302 2637.354 8.336 256.544 1954.502 283.190 9.610 6.750 4.335 4.806 6.928 2.243 0.570 57.72850621 25.740 10.142 2.024 3.890 0.378 3.292 0.355 1.647 0.213 1.039 0.178 1.184 0.050 1.600 0.121 10.155 2.055 1.370 0.393 0.337 0.102 pH8_3 2250.171 2640.562 8.336 256.877 1957.002 283.546 9.622 6.758 4.340 4.744 7.526 2.347 0.563 62.71241744 26.716 pH8_3 2251.687 2643.637 8.338 255.885 1957.379 284.723 9.585 6.787 4.358 4.711 7.793 2.094 0.304 64.93893496 31.010 pH8_3 3922.453 4522.755 8.341 441.755 3406.460 499.445 16.548 11.905 7.645 4.771 7.106 2.441 0.601 59.21666625 24.259 9.834 2.056 3.818 0.381 3.251 0.370 1.611 0.211 1.010 0.176 1.177 0.046 1.610 0.124 9.851 2.092 1.305 0.393 0.295 0.071 pH8_3 3925.105 4524.648 8.340 443.046 3409.606 498.903 16.596 11.892 7.637 4.748 7.323 2.592 0.620 61.02052073 23.546 pH8_3 3934.356 4535.026 8.340 444.088 3417.640 500.081 16.635 11.920 7.655 4.631 7.823 1.914 0.468 65.18633911 34.057 pH8_3 6683.782 7636.011 8.347 740.927 5794.412 861.616 27.755 20.537 13.189 4.867 8.207 2.570 0.556 68.3848939 26.611 9.931 2.095 3.822 0.406 3.270 0.385 1.652 0.219 1.061 0.174 1.171 0.040 1.568 0.095 9.931 2.139 1.404 0.391 0.314 0.124 pH8_3 6680.652 7632.552 8.347 740.582 5791.700 861.211 27.742 20.527 13.183 4.823 8.102 2.281 0.571 67.51553945 29.594 pH8_3 6673.736 7626.511 8.348 738.169 5784.254 861.831 27.651 20.542 13.192 4.759 8.364 2.140 0.473 69.69858812 32.567 pH8_3 9917.417 11219.382 8.328 1155.323 8643.261 1230.879 43.278 29.339 18.842 4.833 9.108 4.230 1.311 75.89437205 17.943 10.062 2.021 3.854 0.375 3.292 0.360 1.491 0.459 0.932 0.292 1.173 0.043 1.608 0.128 10.063 2.075 1.275 0.362 0.368 0.127 pH8_3 9937.891 11239.855 8.327 1160.466 8663.170 1231.251 43.470 29.347 18.847 4.778 8.014 3.050 0.773 66.77868466 21.897 pH8_3 9962.393 11267.189 8.327 1163.445 8684.624 1234.188 43.582 29.417 18.892 4.814 12.189 3.472 0.854 101.5684849 29.251