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Felden, Janine; Wenzhöfer, Frank (2014): Ex situ sulphate reduction and methane oxidation rates of sediment at the rim of a vesicomyd clam colony in the Japan Deep Sea Trench (dive 956) [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.827104, In supplement to: Felden, Janine; Ruff, S Emil; Ertefai, Tobias F; Inagaki, Fumio; Hinrichs, Kai-Uwe; Wenzhöfer, Frank (2014): Anaerobic methanotrophic community of a 5346 m-deep vesicomyid clam colony in the Japan Trench. Geobiology, https://doi.org/10.1111/gbi.12078

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Abstract:
Sediment samples were collected from the rim of a large vesicomyid clam colony in the Japan Deep Sea Trench. Immediately after sample recovery onboard, the sediment core was sub-sampled for ex situ rate measurements. Sulfate reduction and anaerobic oxidation of methane were measured ex situ by the whole core injection method with three replicate measurements for each method. We incubated the samples at in situ temperature (1.5°C) for 48 hours with either 14C-methane (dissolved in water, 2.5 kBq) or carrier-free 35S-sulfate (dissolved in water, 50 kBq). Sediment was fixed in 25 ml sodium hydroxide (NaOH) solution (2.5%, w/v) or 20 ml ZnAc solution (20%, w/v) for AOM or SR, respectively. Turnover rates were measured as previously described (Kallmeyer et al., 2004; Treude et al., 2003).
Further details:
Kallmeyer, Jens; Ferdelman, Timothy G; Weber, Andreas; Fossing, Henrik; Jørgensen, Bo Barker (2004): Evaluation of a cold chromium distillation procedure for recovering very small amounts of radiolabeled sulfide related to sulfate reduction measurements. Limnology and Oceanography-Methods, 2, 171-180, https://doi.org/10.4319/lom.2004.2.171
Treude, Tina; Boetius, Antje; Knittel, Katrin; Wallmann, Klaus; Jørgensen, Bo Barker (2003): Anaerobic oxidation of methane above gas hydrates at Hydrate Ridge, NE Pacific Ocean. Marine Ecology Progress Series, 264, 1-14, https://doi.org/10.3354/meps264001
Coverage:
Latitude: 39.105930 * Longitude: 143.892690
Date/Time Start: 2006-06-07T00:00:00 * Date/Time End: 2006-06-07T00:00:00
Minimum DEPTH, sediment/rock: 0.005 m * Maximum DEPTH, sediment/rock: 0.275 m
Event(s):
6K-956-CR * Latitude: 39.105930 * Longitude: 143.892690 * Date/Time: 2006-06-07T00:00:00 * Elevation: -5346.0 m * Location: Sanriku Escarpment in the Japan Trench * Campaign: YK06-05 * Basis: Yokosuka * Method/Device: Push corer (PUC) * Comment: Submersible Shinkai 6500 (JAMSTEC, Japan), dive 956, push core red, outside mussel bed
6K-956-CRW * Latitude: 39.105930 * Longitude: 143.892690 * Date/Time: 2006-06-07T00:00:00 * Elevation: -5346.0 m * Location: Sanriku Escarpment in the Japan Trench * Campaign: YK06-05 * Basis: Yokosuka * Method/Device: Push corer (PUC) * Comment: Submersible Shinkai 6500 (JAMSTEC, Japan), dive 956, push core red/white, outside mussel bed
6K-956-CW * Latitude: 39.105930 * Longitude: 143.892690 * Date/Time: 2006-06-07T00:00:00 * Elevation: -5346.0 m * Location: Sanriku Escarpment in the Japan Trench * Campaign: YK06-05 * Basis: Yokosuka * Method/Device: Push corer (PUC) * Comment: Submersible Shinkai 6500 (JAMSTEC, Japan), dive 956, push core white, outside mussel bed
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEvent
2DEPTH, sediment/rockDepth sedmGeocode
3Methane, oxidation rate, anaerobicCH4 AOMnmol/cm3/dayFelden, JanineRadioactive tracer injection, Gas Chromatography and scintillation counting
4Sulfate reduction rateSRRnmol/cm3/dayFelden, JanineRadioactive tracer injection, Anion exchange chromatography, scintillation count
Size:
156 data points

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