Citation:

j.marenvres.2012.06.002

Name: Physiological responses of the marine diatom Thalassiosira pseudonana to increased pCO2 and seawater acidity
Published: 2012
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Laboratory experiment; Laboratory strains; Light; North Pacific; Ochrophyta; Phytoplankton; Respiration; Thalassiosira pseudonana

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Published: 2012 (exact date unknown) • DOI registered: 2014-04-15

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Abstract:

We studied the effects of elevated CO2 concentration and seawater acidity on inorganic carbon acquisition, photoinhibition and photoprotection as well as growth and respiration in the marine diatom Thalassiosira pseudonana. After having grown under the elevated CO2 level (1000 µatm, pH 7.83) at sub-saturating photosynthetically active radiation (PAR, 75 µmol photons/m**2/s) for 20 generations, photosynthesis and dark respiration of the alga increased by 25% (14.69 ± 2.55 fmol C/cell/h) and by 35% (4.42 ± 0.98 fmol O2/cell/h), respectively, compared to that grown under the ambient CO2 level (390 µatm, pH 8.16), leading to insignificant effects on growth (1.09 ± 0.08 (1/d))v 1.04 ± 0.07 (1/d)). The photosynthetic affinity for CO2 was lowered in the high-CO2 grown cells, reflecting a down-regulation of the CO2 concentrating mechanism (CCM). When exposed to an excessively high level of PAR, photochemical and non-photochemical quenching responded similarly in the low- and high-CO2 grown cells, reflecting that photoinhibition was not influenced by the enriched level of CO2. In T. pseudonana, it appeared that the energy saved due to the down-regulated CCM did not contribute to any additional light stress as previously found in another diatom Phaeodactylum tricornutum, indicating differential physiological responses to ocean acidification between these two diatom species.

Keyword(s):

Laboratory experiment; Laboratory strains; Light; North Pacific; Ochrophyta; Phytoplankton; Respiration; Thalassiosira pseudonana

Further details:

Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4 [webpage]. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-01-20.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Treatment	Treat		Yang, Gui yuan		LC=Low pCO2, HC=High pCO2
2	Identification	ID		Yang, Gui yuan
3	Species	Species		Yang, Gui yuan
4	Carbon, inorganic, dissolved	DIC	µmol/l	Yang, Gui yuan		in figure 1
5	Irradiance	E	µmol photons/m²/s	Yang, Gui yuan
6	Photosynthetic carbon fixation rate per cell	C fix/cell	fmol/#/h	Yang, Gui yuan
7	Photosynthetic carbon fixation rate, standard deviation	C fix std dev	±	Yang, Gui yuan
8	Maximal electron transport rate, relative	rETR max	µmol e/m²/s	Yang, Gui yuan
9	Electron transport rate, relative, standard deviation	rETR std dev	±	Yang, Gui yuan
10	Carbon, inorganic, dissolved, reciprocal of photosynthetic affinity value	DIC K1/2	mmol/l	Yang, Gui yuan		derived from photosynthesis v DIC curves
11	Carbon, inorganic, dissolved, reciprocal of photosynthetic affinity value, standard deviation	DIC K1/2 std dev	±	Yang, Gui yuan		derived from photosynthesis v DIC curves
12	Carbon, inorganic, dissolved, reciprocal of photosynthetic affinity value	DIC K1/2	mmol/l	Yang, Gui yuan		derived from the rETR v DIC curves
13	Carbon, inorganic, dissolved, reciprocal of photosynthetic affinity value, standard deviation	DIC K1/2 std dev	±	Yang, Gui yuan		derived from the rETR v DIC curves
14	Carbon dioxide, reciprocal of photosynthetic affinity value	CO2 K1/2	µmol/l	Yang, Gui yuan		derived from photosynthesis v DIC curves
15	Carbon dioxide, reciprocal of photosynthetic affinity value, standard deviation	CO2 K1/2 std dev	±	Yang, Gui yuan		derived from photosynthesis v DIC curves
16	Carbon dioxide, reciprocal of photosynthetic affinity value	CO2 K1/2	µmol/l	Yang, Gui yuan		derived from the rETR v DIC curves
17	Carbon dioxide, reciprocal of photosynthetic affinity value, standard deviation	CO2 K1/2 std dev	±	Yang, Gui yuan		derived from the rETR v DIC curves
18	Photosynthetic carbon fixation per cell, maximum velocity	C fix Vmax/cell	fmol/#/h	Yang, Gui yuan
19	Photosynthetic carbon fixation, maximum velocity, standard deviation	C fix Vmax std dev	±	Yang, Gui yuan
20	Electron transport rate, relative, maximum velocity	rETR Vmax	arbitrary units	Yang, Gui yuan
21	Electron transport rate, relative, maximum velocity, standard deviation	rETR Vmax std dev	±	Yang, Gui yuan
22	Growth rate	µ	1/day	Yang, Gui yuan
23	Growth rate, standard deviation	µ std dev	±	Yang, Gui yuan
24	Chlorophyll a per cell	Chl a/cell	pg/#	Yang, Gui yuan
25	Chlorophyll a, standard deviation	Chl a std dev	±	Yang, Gui yuan
26	Chlorophyll c per cell	Chl c/cell	pg/#	Yang, Gui yuan
27	Chlorophyll c, standard deviation	Chl c std dev	±	Yang, Gui yuan
28	Gross carbon fixation rate per cell	G C fix/cell	fmol/#/day	Yang, Gui yuan
29	Gross carbon fixation rate, standard deviation	G C fix std dev	±	Yang, Gui yuan
30	Respiration rate, oxygen, per cell	Resp O2/cell	fmol/#/h	Yang, Gui yuan
31	Respiration rate, oxygen, standard deviation	Resp O2 std dev	±	Yang, Gui yuan
32	Photosynthetic efficiency	Fv/Fm		Yang, Gui yuan		derived from photosynthesis v DIC curves
33	Photosynthetic efficiency, standard deviation	alpha std dev	±	Yang, Gui yuan		derived from photosynthesis v DIC curves
34	Photosynthetic efficiency	Fv/Fm		Yang, Gui yuan		derived from the rETR v DIC curves
35	Photosynthetic efficiency, standard deviation	alpha std dev	±	Yang, Gui yuan		derived from the rETR v DIC curves
36	Light saturation point	Ik		Yang, Gui yuan		derived from photosynthesis v DIC curves
37	Light saturation point, standard deviation	Ik std dev	±	Yang, Gui yuan		derived from photosynthesis v DIC curves
38	Light saturation point	Ik		Yang, Gui yuan		derived from the rETR v DIC curves
39	Light saturation point, standard deviation	Ik std dev	±	Yang, Gui yuan		derived from the rETR v DIC curves
40	Time in seconds	Time sec	s	Yang, Gui yuan
41	Effective quantum yield	Y		Yang, Gui yuan
42	Effective quantum yield, standard deviation	Y std dev	±	Yang, Gui yuan
43	Effective absorbance cross-section of photosystem II	sigma PSII	A²/quanta	Yang, Gui yuan
44	Effective absorbance cross-section of photosystem II, standard deviation	sigma PSII std dev	±	Yang, Gui yuan
45	Non photochemical quenching	NPQ		Yang, Gui yuan
46	Non photochemical quenching, standard deviation	NPQ std dev	±	Yang, Gui yuan
47	Temperature, water	Temp	°C	Yang, Gui yuan		in the middle of the light period
48	Salinity	Sal		Yang, Gui yuan		in the middle of the light period
49	pH, NBS scale	pH NBS		Yang, Gui yuan		in the middle of the light period, NBS scale
50	pH, standard deviation	pH std dev	±	Yang, Gui yuan		in the middle of the light period, NBS scale
51	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Gui yuan		in the middle of the light period
52	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Yang, Gui yuan		in the middle of the light period
53	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Gui yuan	Calculated using CO2SYS	in the middle of the light period
54	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Yang, Gui yuan	Calculated using CO2SYS	in the middle of the light period
55	Carbonate ion	[CO3]2-	µmol/kg	Yang, Gui yuan	Calculated using CO2SYS	in the middle of the light period
56	Carbonate ion, standard deviation	[CO3]2- std dev	±	Yang, Gui yuan	Calculated using CO2SYS	in the middle of the light period
57	Carbon dioxide	CO2	µmol/kg	Yang, Gui yuan	Calculated using CO2SYS	in the middle of the light period
58	Carbon dioxide, standard deviation	CO2 std dev	±	Yang, Gui yuan	Calculated using CO2SYS	in the middle of the light period
59	Alkalinity, total	AT	µmol/kg	Yang, Gui yuan	Calculated using CO2SYS	in the middle of the light period
60	Alkalinity, total, standard deviation	AT std dev	±	Yang, Gui yuan	Calculated using CO2SYS	in the middle of the light period
61	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
62	pH, total scale	pHT		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
63	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
64	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
65	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
66	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
67	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
68	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
69	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
70	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
71	pH, NBS scale	pH NBS		Yang, Gui yuan		pCO2 switched, NBS scale
72	pH, standard deviation	pH std dev	±	Yang, Gui yuan		pCO2 switched, NBS scale
73	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Gui yuan		pCO2 switched
74	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Yang, Gui yuan		pCO2 switched
75	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Gui yuan	Calculated using CO2SYS	pCO2 switched
76	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Yang, Gui yuan	Calculated using CO2SYS	pCO2 switched
77	Carbonate ion	[CO3]2-	µmol/kg	Yang, Gui yuan	Calculated using CO2SYS	pCO2 switched
78	Carbonate ion, standard deviation	[CO3]2- std dev	±	Yang, Gui yuan	Calculated using CO2SYS	pCO2 switched
79	Carbon dioxide	CO2	µmol/kg	Yang, Gui yuan	Calculated using CO2SYS	pCO2 switched
80	Carbon dioxide, standard deviation	CO2 std dev	±	Yang, Gui yuan	Calculated using CO2SYS	pCO2 switched
81	Alkalinity, total	AT	µmol/kg	Yang, Gui yuan	Calculated using CO2SYS	pCO2 switched
82	Alkalinity, total, standard deviation	AT std dev	±	Yang, Gui yuan	Calculated using CO2SYS	pCO2 switched
83	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	pCO2 switched
84	pH, total scale	pHT		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	pCO2 switched, total scale
85	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	pCO2 switched
86	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	pCO2 switched
87	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	pCO2 switched
88	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	pCO2 switched
89	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	pCO2 switched
90	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	pCO2 switched
91	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	pCO2 switched
92	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	pCO2 switched

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

4682 data points

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