Citation:

mms.553

Name: Seawater carbonate chemistry and egg production, hatching and metabolic rates of a Mediterranean copepod species (Acartia clausi) in a laboratory experiment
Published: 2014-01-11
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Acartia clausi; Animalia; Arthropoda; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Mediterranean Sea; Other metabolic rates; Pelagos; Reproduction; Respiration; Single species; Temperate; Temperature; Zooplankton

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Abstract:

This study includes the first information on the combined effect of low pH and raised temperature on egg production rate (EP), hatching success (HS), excretion and respiration of the Mediterranean copepod Acartia clausi. Adult individuals of A. clausi and fresh surface seawater were collected at a coastal station in Saronikos Gulf during April 2012. Four different conditions were applied: two different pH levels (present: 8.09 and future: 7.83) at two temperature values (present: 16°C and present+4 °C= 20°C). EP and HS success decreased significantly over the duration of exposure at future pH at both temperature conditions. However, the analysis of the combined effect of pH, T, chlorophyll a and the duration of the experiments on EP and HS revealed that ocean acidification had no discernible effect, whereas warming; food and the duration of exposure were more significant for the reproductive output of A. clausi. Temperature appeared to have a positive effect on respiration and excretion. Acidification had no clear effect on respiration, but a negative effect on the A. clausi excretion was observed. Acidification and warming resulted in the increase of the excretion rate and the increase was higher than that observed by warming only. Our findings showed that a direct effect of ocean acidification on copepod's vital rates was not obvious, except maybe in the case of excretion. Therefore, the combination of acidification with the ambient oligotrophic conditions and the warming could result in species being less able to allocate resources for coping with multiple stressors.

Keyword(s):

Acartia clausi; Animalia; Arthropoda; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Mediterranean Sea; Other metabolic rates; Pelagos; Reproduction; Respiration; Single species; Temperate; Temperature; Zooplankton

Further details:

Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb

Project(s):

Mediterranean Sea Acidification in a Changing Climate (MedSeA)

Ocean Acidification International Coordination Centre (OA-ICC)

Funding:

Seventh Framework Programme (FP7), grant/award no. 265103 : Mediterranean Sea Acidification in a Changing Climate

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-12-18.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Treatment	Treat		Zervoudaki, Soultana
2	Identification	ID		Zervoudaki, Soultana
3	Species	Species		Zervoudaki, Soultana
4	Temperature, water	Temp	°C	Zervoudaki, Soultana
5	Salinity	Sal		Zervoudaki, Soultana
6	Light:Dark cycle	L:D	hh:hh	Zervoudaki, Soultana
7	pH	pH		Krasakopoulou, Evangelia	Potentiometric	total scale
8	Carbon, inorganic, dissolved	DIC	µmol/kg	Giannoudi, Louisa	Coulometric titration
9	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Giannoudi, Louisa	Coulometric titration
10	Ammonia excretion per individual	NH3/[NH4]+ exc/ind	µg/#/day	Frangoulis, Constantin
11	Ammonium, excretion, standard deviation	[NH4]+ exc std dev	±	Frangoulis, Constantin
12	Respiration rate, oxygen, per individual	Resp O2/ind	µl/#/day	Frangoulis, Constantin
13	Respiration rate, oxygen, standard deviation	Resp O2 std dev	±	Frangoulis, Constantin
14	Egg production rate per female	EPR	#/female/day	Zervoudaki, Soultana
15	Egg production rate, standard deviation	EPR std dev	±	Zervoudaki, Soultana
16	Hatching rate	Hatching	%	Zervoudaki, Soultana
17	Hatching rate, standard deviation	Hatching std dev	±	Zervoudaki, Soultana
18	Carbon dioxide	CO2	µmol/kg	Krasakopoulou, Evangelia	Calculated using seacarb
19	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Krasakopoulou, Evangelia	Calculated using seacarb
20	Bicarbonate ion	[HCO3]-	µmol/kg	Krasakopoulou, Evangelia	Calculated using seacarb
21	Carbonate ion	[CO3]2-	µmol/kg	Krasakopoulou, Evangelia	Calculated using seacarb
22	Alkalinity, total	AT	µmol/kg	Krasakopoulou, Evangelia	Calculated using seacarb
23	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
24	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
25	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
26	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
27	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
28	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
29	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
31	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

862 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Treat	2 ID	3 Species	4 Temp [°C]	5 Sal	6 L:D [hh:hh]	7 pH (total scale, Potentiometric)	8 DIC [µmol/kg] (Coulometric titration)	9 DIC std dev [±] (Coulometric titration)	10 NH3/[NH4]+ exc/ind [µg/#/day]	11 [NH4]+ exc std dev [±]	12 Resp O2/ind [µl/#/day]	13 Resp O2 std dev [±]	14 EPR [#/female/day]	15 EPR std dev [±]	16 Hatching [%]	17 Hatching std dev [±]	18 CO2 [µmol/kg] (Calculated using seacarb)	19 pCO2water_SST_wet [µatm] (Calculated using seacarb)	20 [HCO3]- [µmol/kg] (Calculated using seacarb)	21 [CO3]2- [µmol/kg] (Calculated using seacarb)	22 AT [µmol/kg] (Calculated using seacarb)	23 CSC flag (Calculated using seacarb afte...)	24 CO2 [µmol/kg] (Calculated using seacarb afte...)	25 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	26 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	27 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	28 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	29 AT [µmol/kg] (Calculated using seacarb afte...)	30 Omega Arg (Calculated using seacarb afte...)	31 Omega Cal (Calculated using seacarb afte...)
pH1T1	Day1	Acartia clausi (copepod)	16.000	38.700	14:10	8.04	2401	4					5.2	0.7	35.3	3.8	16.5	464	2183	201	2677	9	17	465	464	2184	201	2673	3.01	4.65
pH1T2	Day1	Acartia clausi (copepod)	20.000	38.700	14:10	8.00	2299	3					2.9	0.4	44.4	7.0	15.8	499	2081	203	2580	9	16	499	497	2080	203	2576	3.07	4.71
pH2T1	Day1	Acartia clausi (copepod)	16.000	38.700	14:10	7.83	2460	4					4.9	0.5	59.3	5.0	28.2	793	2302	131	2623	9	28	795	792	2301	130	2620	1.95	3.02
pH2T2	Day1	Acartia clausi (copepod)	20.000	38.700	14:10	7.83	2429	5					5.2	0.3	73.3	4.8	25.2	795	2254	149	2620	9	25	799	797	2255	149	2617	2.25	3.45
pH1T1	Day2	Acartia clausi (copepod)	16.000	38.700	14:10	8.04	2457	3					4.3	0.9	51.0	7.6	16.8	474	2234	206	2737	9	17	476	474	2235	205	2733	3.08	4.76
pH1T2	Day2	Acartia clausi (copepod)	20.000	38.700	14:10	8.04	2245	4					3.8	0.9	68.8	2.4	13.9	440	2016	215	2547	9	14	441	439	2016	215	2544	3.26	5.00
pH2T1	Day2	Acartia clausi (copepod)	16.000	38.700	14:10	7.83	2496	6					3.8	0.6	55.0	6.4	28.6	805	2335	132	2660	9	29	807	804	2335	132	2658	1.98	3.07
pH2T2	Day2	Acartia clausi (copepod)	20.000	38.700	14:10	7.83	2460	5					3.7	0.4	71.0	6.4	25.5	806	2283	151	2653	9	26	809	807	2284	150	2650	2.28	3.49
pH1T1	Day3	Acartia clausi (copepod)	16.000	38.800	14:10	8.04	2403	3					4.6	0.5	64.0	2.2	16.5	465	2185	201	2679	9	17	466	464	2185	201	2676	3.01	4.66
pH1T2	Day3	Acartia clausi (copepod)	20.000	38.800	14:10	8.00	2313	2					3.4	0.4	49.0	4.3	15.9	502	2093	204	2595	9	16	501	500	2093	204	2592	3.09	4.74
pH2T1	Day3	Acartia clausi (copepod)	16.000	38.800	14:10	7.83	2544	7					2.1	0.5	41.9	5.7	29.1	820	2380	135	2710	9	29	822	819	2380	135	2708	2.02	3.13
pH2T2	Day3	Acartia clausi (copepod)	20.000	38.800	14:10	7.83	2493	4					3.9	0.5	71.5	9.9	26.0	820	2315	152	2687	9	26	820	817	2314	153	2685	2.31	3.55
pH1T1	Day4	Acartia clausi (copepod)	16.000	38.700	14:10	8.04	2398	3					3.0	0.7	38.6	10.6	16.5	464	2181	201	2674	9	16	465	463	2181	200	2670	3.00	4.65
pH1T2	Day4	Acartia clausi (copepod)	20.000	38.700	14:10	8.04	2390	3					3.4	0.3	54.3	5.4	14.8	468	2145	230	2706	9	15	469	467	2146	229	2702	3.47	5.32
pH2T1	Day4	Acartia clausi (copepod)	16.000	38.700	14:10	7.83	2537	6					2.3	0.3	25.0	4.0	29.1	820	2374	134	2703	9	29	820	817	2374	134	2700	2.01	3.12
pH2T2	Day4	Acartia clausi (copepod)	20.000	38.700	14:10	7.83	2565	6					1.8	0.2	54.0	6.1	26.7	844	2381	157	2762	9	27	844	841	2381	157	2760	2.38	3.64
pH1T1	Day5	Acartia clausi (copepod)	16.000	38.800	14:10	8.04	2382	4					2.3	0.7	37.8	9.2	16.4	462	2167	199	2656	9	16	462	460	2166	199	2653	2.98	4.62
pH1T2	Day5	Acartia clausi (copepod)	20.000	38.800	14:10	8.00	2382	3					1.6	0.4	42.6	10.1	16.4	517	2156	210	2669	9	16	516	515	2155	210	2667	3.19	4.88
pH2T1	Day5	Acartia clausi (copepod)	16.000	38.800	14:10	7.83	2513	5					1.7	0.2	32.4	2.0	28.8	812	2350	133	2677	9	29	812	809	2351	133	2676	2.00	3.09
pH2T2	Day5	Acartia clausi (copepod)	20.000	38.800	14:10	7.83	2552	6					1.7	0.1	44.4	7.9	26.6	840	2369	156	2749	9	27	840	837	2369	156	2747	2.37	3.63
pH1T1	exp A	Acartia clausi (copepod)	16.000	38.700	14:10	8.04	2484	3	0.04597	0.024300	0.97	0.29					17.1	481	2260	207	2766	9	17	481	480	2259	207	2762	3.11	4.81
pH1T2	exp A	Acartia clausi (copepod)	20.000	38.700	14:10	8.00	2238	4	0.04909	0.011230	1.08	0.52					15.4	485	2025	197	2514	9	15	485	484	2025	197	2510	2.99	4.58
pH2T1	exp A	Acartia clausi (copepod)	16.000	38.700	14:10	7.83	2628	6	0.01972	0.010010	1.01	0.16					30.2	850	2459	139	2798	9	30	850	847	2459	139	2795	2.09	3.23
pH2T2	exp A	Acartia clausi (copepod)	20.000	38.700	14:10	7.83	2406	5			1.10	0.28					25.1	792	2234	147	2595	9	25	792	789	2234	147	2593	2.23	3.42
pH1T1	exp B	Acartia clausi (copepod)	16.000	38.800	14:10	8.04	2456	4	0.05177	0.008640	0.79	0.25					16.9	476	2234	205	2735	9	17	476	474	2234	206	2733	3.08	4.76
pH1T2	exp B	Acartia clausi (copepod)	20.000	38.800	14:10	8.00	2456	4	0.07030	0.009170	1.28	0.58					16.9	533	2223	217	2750	9	17	532	531	2222	217	2747	3.28	5.04
pH2T1	exp B	Acartia clausi (copepod)	16.000	38.800	14:10	7.83	2559	5	0.02526	0.012150	1.02	0.41					29.3	825	2394	136	2726	9	29	827	824	2394	136	2724	2.03	3.15
pH2T2	exp B	Acartia clausi (copepod)	20.000	38.800	14:10	7.83	2415	4	0.10059	0.053310	1.06	0.58					25.1	793	2242	148	2605	9	25	795	792	2242	148	2603	2.24	3.43
pH1T1	exp C	Acartia clausi (copepod)	16.000	38.700	14:10	8.06	2397	4	0.03058	0.030710	0.50	0.23					15.7	442	2172	209	2685	9	16	442	440	2172	209	2681	3.13	4.85
pH1T2	exp C	Acartia clausi (copepod)	20.000	38.700	14:10	8.04	2336	3	0.04605	0.036460	1.40	0.44					14.4	456	2097	225	2648	9	15	458	457	2097	224	2643	3.39	5.20
pH2T1	exp C	Acartia clausi (copepod)	16.000	38.700	14:10	7.83	2580	5	0.00756	0.003120	0.41	0.38					29.3	826	2413	138	2749	9	30	834	831	2414	137	2745	2.05	3.17
pH2T2	exp C	Acartia clausi (copepod)	20.000	38.700	14:10	7.83	2574	6	0.08686	0.016150	0.87	0.31					26.7	845	2389	158	2772	9	27	847	844	2390	157	2769	2.38	3.66