Citation:

journal.pone.0070455.t001

Name: Seawater carbonate chemistry and growth, reproduction of calcifying and non-calcifying epibionts of a brown macroalga in a laboratory experiment
Published: 2013
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Alcyonidium hirsutum; Animalia; Baltic Sea; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Electra pilosa; Growth/Morphology; Laboratory experiment; Reproduction; Single species; Spirorbis spirorbis; Temperate

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Abstract:

Seaweeds are key species of the Baltic Sea benthic ecosystems. They are the substratum of numerous fouling epibionts like bryozoans and tubeworms. Several of these epibionts bear calcified structures and could be impacted by the high pCO2 events of the late summer upwellings in the Baltic nearshores. Those events are expected to increase in strength and duration with global change and ocean acidification. If calcifying epibionts are impacted by transient acidification as driven by upwelling events, their increasing prevalence could cause a shift of the fouling communities toward fleshy species. The aim of the present study was to test the sensitivity of selected seaweed macrofoulers to transient elevation of pCO2 in their natural microenvironment, i.e. the boundary layer covering the thallus surface of brown seaweeds. Fragments of the macroalga Fucus serratus bearing an epibiotic community composed of the calcifiers Spirorbis spirorbis (Annelida) and Electra pilosa (Bryozoa) and the non-calcifier Alcyonidium hirsutum (Bryozoa) were maintained for 30 days under three pCO2 conditions: natural 460±59 µatm, present-day upwelling1193±166 µatm and future upwelling 3150±446 µatm. Only the highest pCO2 caused a significant reduction of growth rates and settlement of S. spirorbis individuals. Additionally, S. spirorbis settled juveniles exhibited enhanced calcification of 40% during daylight hours compared to dark hours, possibly reflecting a day-night alternation of an acidification-modulating effect by algal photosynthesis as opposed to an acidification-enhancing effect of algal respiration. E. pilosa colonies showed significantly increased growth rates at intermediate pCO2 (1193 µatm) but no response to higher pCO2. No effect of acidification on A. hirsutum colonies growth rates was observed. The results suggest a remarkable resistance of the algal macro-epibionts to levels of acidification occurring at present day upwellings in the Baltic. Only extreme future upwelling conditions impacted the tubeworm S. spirorbis, but not the bryozoans.

Keyword(s):

Alcyonidium hirsutum; Animalia; Baltic Sea; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Electra pilosa; Growth/Morphology; Laboratory experiment; Reproduction; Single species; Spirorbis spirorbis; Temperate

Further details:

Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 54.450000 * Longitude: 9.883330

Date/Time Start: 2011-02-01T00:00:00 * Date/Time End: 2011-02-28T00:00:00

Minimum Elevation: -2.0 m * Maximum Elevation: 0.0 m

Event(s):

Eckernforder_Bay_OA * Latitude: 54.450000 * Longitude: 9.883330 * Date/Time Start: 2011-02-01T00:00:00 * Date/Time End: 2011-02-28T00:00:00 * Elevation Start: 0.0 m * Elevation End: -2.0 m * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-12-10.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Identification	ID		Saderne, Vincent
2	Species	Species		Saderne, Vincent
3	Treatment	Treat		Saderne, Vincent
4	Irradiance	E	µmol/m²/s	Saderne, Vincent
5	Sample code/label	Sample label		Saderne, Vincent
6	Growth	Growth	%	Saderne, Vincent		growth of the bryozoan during the 30 days of incubations
7	Individuals, adult	Ind ad	#	Saderne, Vincent
8	Individuals, juvenile	Ind juv	#	Saderne, Vincent		settled
9	Juveniles, settled	Juv sett	%	Saderne, Vincent		per worm
10	Growth rate	µ	mm/day	Saderne, Vincent		adult
11	Growth rate	µ	mm/day	Saderne, Vincent		juveniles
12	Temperature, water	Temp	°C	Saderne, Vincent
13	Temperature, water, standard deviation	Temp std dev	±	Saderne, Vincent
14	Salinity	Sal		Saderne, Vincent
15	Salinity, standard deviation	Sal std dev	±	Saderne, Vincent
16	pH	pH		Saderne, Vincent	Potentiometric	total scale
17	pH, standard deviation	pH std dev	±	Saderne, Vincent	Potentiometric	total scale
18	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Saderne, Vincent	Calculated using seacarb
19	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Saderne, Vincent	Calculated using seacarb
20	Carbon, inorganic, dissolved	DIC	µmol/kg	Saderne, Vincent
21	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Saderne, Vincent
22	Alkalinity, total	AT	µmol/kg	Saderne, Vincent	Calculated using seacarb
23	Alkalinity, total, standard deviation	AT std dev	±	Saderne, Vincent	Calculated using seacarb
24	Calcite saturation state	Omega Cal		Saderne, Vincent	Calculated using seacarb
25	Calcite saturation state, standard deviation	Omega Cal std dev	±	Saderne, Vincent	Calculated using seacarb
26	Aragonite saturation state	Omega Arg		Saderne, Vincent	Calculated using seacarb
27	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Saderne, Vincent	Calculated using seacarb
28	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
29	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
31	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

4408 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 ID	2 Species	3 Treat	4 E [µmol/m²/s]	5 Sample label	6 Growth [%] (growth of the bryozoan during...)	7 Ind ad [#]	8 Ind juv [#] (settled)	9 Juv sett [%] (per worm)	10 µ [mm/day] (adult)	11 µ [mm/day] (juveniles)	12 Temp [°C]	13 Temp std dev [±]	14 Sal	15 Sal std dev [±]	16 pH (total scale, Potentiometric)	17 pH std dev [±] (total scale, Potentiometric)	18 pCO2water_SST_wet [µatm] (Calculated using seacarb)	19 pCO2 std dev [±] (Calculated using seacarb)	20 DIC [µmol/kg]	21 DIC std dev [±]	22 AT [µmol/kg] (Calculated using seacarb)	23 AT std dev [±] (Calculated using seacarb)	24 Omega Cal (Calculated using seacarb)	25 Omega Cal std dev [±] (Calculated using seacarb)	26 Omega Arg (Calculated using seacarb)	27 Omega Arg std dev [±] (Calculated using seacarb)	28 CSC flag (Calculated using seacarb afte...)	29 CO2 [µmol/kg] (Calculated using seacarb afte...)	30 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	31 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	32 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	33 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	34 AT [µmol/kg] (Calculated using seacarb afte...)	35 Omega Arg (Calculated using seacarb afte...)	36 Omega Cal (Calculated using seacarb afte...)
Figure 5	Alcyonidium hirsutum (Bryozoa)	pCO2=460 micro atm		bottle 1	14.64						16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 5	Alcyonidium hirsutum (Bryozoa)	pCO2=460 micro atm		bottle 2	55.37						16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 5	Alcyonidium hirsutum (Bryozoa)	pCO2=460 micro atm		bottle 3	3.12						16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 5	Alcyonidium hirsutum (Bryozoa)	pCO2=460 micro atm		bottle 4	24.85						16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 5	Alcyonidium hirsutum (Bryozoa)	pCO2=460 micro atm		bottle 5	8.79						16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 5	Alcyonidium hirsutum (Bryozoa)	pCO2=460 micro atm		bottle 6	23.43						16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 5	Alcyonidium hirsutum (Bryozoa)	pCO2=460 micro atm		bottle 7	11.82						16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 5	Alcyonidium hirsutum (Bryozoa)	pCO2=460 micro atm		bottle 8	81.42						16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 5	Alcyonidium hirsutum (Bryozoa)	pCO2=460 micro atm		bottle 9	21.10						16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 5	Alcyonidium hirsutum (Bryozoa)	pCO2=1200 micro atm		bottle 1	6.43						15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 5	Alcyonidium hirsutum (Bryozoa)	pCO2=1200 micro atm		bottle 2	20.24						15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 5	Alcyonidium hirsutum (Bryozoa)	pCO2=1200 micro atm		bottle 3	10.38						15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 5	Alcyonidium hirsutum (Bryozoa)	pCO2=1200 micro atm		bottle 4	11.91						15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 5	Alcyonidium hirsutum (Bryozoa)	pCO2=1200 micro atm		bottle 5	58.24						15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 5	Alcyonidium hirsutum (Bryozoa)	pCO2=1200 micro atm		bottle 6	68.24						15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 5	Alcyonidium hirsutum (Bryozoa)	pCO2=1200 micro atm		bottle 7	116.27						15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 5	Alcyonidium hirsutum (Bryozoa)	pCO2=3150 micro atm		bottle 1	13.42						15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 5	Alcyonidium hirsutum (Bryozoa)	pCO2=3150 micro atm		bottle 2	4.29						15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 5	Alcyonidium hirsutum (Bryozoa)	pCO2=3150 micro atm		bottle 3	7.16						15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 5	Alcyonidium hirsutum (Bryozoa)	pCO2=3150 micro atm		bottle 4	54.32						15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 5	Alcyonidium hirsutum (Bryozoa)	pCO2=3150 micro atm		bottle 5	17.93						15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 5	Alcyonidium hirsutum (Bryozoa)	pCO2=3150 micro atm		bottle 6	34.05						15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 5	Alcyonidium hirsutum (Bryozoa)	pCO2=3150 micro atm		bottle 7	22.28						15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 5	Electra pilosa (Bryozoa)	pCO2=460 micro atm		bottle 1	64.21						16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 5	Electra pilosa (Bryozoa)	pCO2=460 micro atm		bottle 2	72.17						16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 5	Electra pilosa (Bryozoa)	pCO2=460 micro atm		bottle 3	21.26						16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 5	Electra pilosa (Bryozoa)	pCO2=460 micro atm		bottle 4	74.12						16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 5	Electra pilosa (Bryozoa)	pCO2=460 micro atm		bottle 5	51.58						16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 5	Electra pilosa (Bryozoa)	pCO2=460 micro atm		bottle 6	45.03						16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 5	Electra pilosa (Bryozoa)	pCO2=460 micro atm		bottle 7	25.31						16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 5	Electra pilosa (Bryozoa)	pCO2=460 micro atm		bottle 8	45.77						16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 5	Electra pilosa (Bryozoa)	pCO2=460 micro atm		bottle 9	62.85						16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 5	Electra pilosa (Bryozoa)	pCO2=460 micro atm		bottle 10	44.74						16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 5	Electra pilosa (Bryozoa)	pCO2=1200 micro atm		bottle 1	50.84						15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 5	Electra pilosa (Bryozoa)	pCO2=1200 micro atm		bottle 2	65.50						15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 5	Electra pilosa (Bryozoa)	pCO2=1200 micro atm		bottle 3	59.57						15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 5	Electra pilosa (Bryozoa)	pCO2=1200 micro atm		bottle 4	47.93						15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 5	Electra pilosa (Bryozoa)	pCO2=1200 micro atm		bottle 5	117.30						15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 5	Electra pilosa (Bryozoa)	pCO2=1200 micro atm		bottle 6	52.49						15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 5	Electra pilosa (Bryozoa)	pCO2=1200 micro atm		bottle 7	71.59						15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 5	Electra pilosa (Bryozoa)	pCO2=1200 micro atm		bottle 8	73.08						15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 5	Electra pilosa (Bryozoa)	pCO2=1200 micro atm		bottle 9	80.17						15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 5	Electra pilosa (Bryozoa)	pCO2=1200 micro atm		bottle 10	146.47						15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 5	Electra pilosa (Bryozoa)	pCO2=3150 micro atm		bottle 1	19.28						15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 5	Electra pilosa (Bryozoa)	pCO2=3150 micro atm		bottle 2	13.78						15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 5	Electra pilosa (Bryozoa)	pCO2=3150 micro atm		bottle 3	38.14						15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 5	Electra pilosa (Bryozoa)	pCO2=3150 micro atm		bottle 4	36.34						15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 5	Electra pilosa (Bryozoa)	pCO2=3150 micro atm		bottle 5	56.51						15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 5	Electra pilosa (Bryozoa)	pCO2=3150 micro atm		bottle 6	24.83						15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 5	Electra pilosa (Bryozoa)	pCO2=3150 micro atm		bottle 7	42.09						15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 5	Electra pilosa (Bryozoa)	pCO2=3150 micro atm		bottle 8	67.25						15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 5	Electra pilosa (Bryozoa)	pCO2=3150 micro atm		bottle 9	119.17						15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 5	Electra pilosa (Bryozoa)	pCO2=3150 micro atm		bottle 10	12.26						15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm		bottle 1		25			0.01995		16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm		bottle 2		20			0.02097		16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm		bottle 3		5			0.03931		16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm		bottle 4		6			0.04239		16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm		bottle 5		22			0.03244		16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm		bottle 6		30			0.02670		16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm		bottle 7		4			0.04914		16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm		bottle 8		8			0.02254		16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm		bottle 9		9			0.02080		16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm		bottle 10		9			0.04092		16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm		bottle 1		46			0.02010		15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm		bottle 2		37			0.02491		15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm		bottle 3		28			0.02748		15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm		bottle 4		25			0.02429		15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm		bottle 5		14			0.02859		15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm		bottle 6		24			0.02903		15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm		bottle 7		32			0.02897		15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm		bottle 8		14			0.02439		15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm		bottle 9		35			0.02803		15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm		bottle 10		18			0.04029		15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm		bottle 1		21			0.01200		15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm		bottle 2		53			0.01063		15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm		bottle 3		11			0.02440		15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm		bottle 4		43			0.00996		15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm		bottle 5		35			0.01776		15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm		bottle 6		31			0.02731		15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm		bottle 7		37			0.01882		15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm		bottle 8		12			0.02924		15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm		bottle 9		9			0.02875		15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm		bottle 10		15			0.01336		15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm		bottle 1		14	149	10.64			16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm		bottle 2		21	311	14.81			16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm		bottle 3		6	27	4.50			16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm		bottle 4		6	75	12.50			16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm		bottle 5		18	206	11.44			16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm		bottle 6		31	172	5.55			16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm		bottle 7		4	15	3.75			16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm		bottle 8		7	32	4.57			16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm		bottle 9		11	189	17.18			16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm		bottle 10		11	94	8.55			16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm		bottle 1		51	466	9.14			15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm		bottle 2		38	116	3.05			15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm		bottle 3		28	237	8.46			15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm		bottle 4		27	16	0.59			15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm		bottle 5		15	74	4.93			15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm		bottle 6		22	258	11.73			15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm		bottle 7		32	204	6.38			15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm		bottle 8		13	317	24.38			15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm		bottle 9		31	77	2.48			15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm		bottle 10		19	99	5.21			15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm		bottle 1		22	21	0.95			15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm		bottle 2		65	10	0.15			15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm		bottle 3		10	33	3.30			15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm		bottle 4		52	181	3.48			15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm		bottle 5		42	119	2.83			15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm		bottle 6		33	0	0.00			15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm		bottle 7		34	140	4.12			15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm		bottle 8		13	4	0.31			15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm		bottle 9		10	17	1.70			15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 3	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm		bottle 10		19	16	0.84			15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm	300			424				0.07047	16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm	300			424				0.08424	16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm	300			424				0.08590	16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm	300			424				0.09357	16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm	300			424				0.04960	16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm	300			873				0.08745	15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm	300			873				0.05666	15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm	300			873				0.08198	15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm	300			873				0.07070	15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm	300			873				0.05381	15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm	300			232				0.04730	15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm	300			232				0.06611	15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm	300			232				0.07899	15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm	300			232				0.12430	15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm	300			232				0.10144	15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm	0			477				0.06608	16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm	0			477				0.03682	16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm	0			477				0.06721	16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm	0			477				0.04948	16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=460 micro atm	0			477				0.03753	16.1	0.7	16.7	0.4	8.105	0.031	450	59	1966	40	2089	52	3.0	0.3	1.8	0.2	9	16	389	387	1841	110	2093	1.75	2.93
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm	0			653				0.03897	15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm	0			653				0.03155	15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm	0			653				0.05132	15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm	0			653				0.03142	15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=1200 micro atm	0			653				0.06607	15.7	0.5	16.7	0.4	7.726	0.076	1193	166	2115	62	2135	71	1.4	0.2	0.8	0.1	9	41	1020	1016	2024	50	2138	0.79	1.33
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm	0			142					15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm	0			142				0.04288	15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm	0			142					15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm	0			142				0.03253	15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58
Figure 4	Spirorbis spirorbis (Annelida)	pCO2=3150 micro atm	0			142				0.05728	15.9	0.8	16.8	0.4	7.334	0.079	3150	445	2306	251	2298	57	0.7	0.1	0.4	0.1	9	109	2703	2694	2175	22	2226	0.35	0.58