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Töbe, Kerstin; Alpermann, Tilman J; Tillmann, Urban; Krock, Bernd; Cembella, Allan; John, Uwe (2013): (Table S2) Cell concentrations of individual and combined Alexandrium spp. and phylogenetic groups as obtained by qPCR assay and Utermöhl counts, as well as particulate PSP toxin concentrations from discrete water depths. PANGAEA, https://doi.org/10.1594/PANGAEA.820036, Supplement to: Töbe, K et al. (2013): Molecular discrimination of toxic and non-toxic Alexandrium species (Dinophyta) in natural phytoplankton assemblages from the Scottish coast of the North Sea. European Journal of Phycology, 48(1), 12-26, https://doi.org/10.1080/09670262.2012.752870

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Abstract:
Molecular methods provide promising tools for routine detection and quantification of toxic microalgae in plankton samples. To this end, novel TaqMan minor groove binding probes and primers targeting the small (SSU) or large (LSU) ribosomal subunit (rRNA) were developed for two species of the marine dinoflagellate genus Alexandrium (A. minutum, A. tamutum) and for three groups/ribotypes of the A. tamarense species complex: Group I/North American (NA), Group II/Mediterranean (ME) and Group III/Western European (WE). Primers and probes for real-time quantitative PCR (qPCR) were species-specific and highly efficient when tested in qPCR assays for cross-validation with pure DNA from cultured Alexandrium strains. Suitability of the qPCR assays as molecular tools for the detection and estimation of relative cell abundances of Alexandrium species and groups was evaluated from samples of natural plankton assemblages along the Scottish east coast. The results were compared with inverted microscope cell counts (Utermöhl technique) of Alexandrium spp. and associated paralytic shellfish poisoning (PSP) toxin concentrations. The qPCR assays indicated that A. tamarense (Group I) and A. tamutum were the most abundant Alexandrium taxa and both were highly positively correlated with PSP toxin content of plankton samples. Cells of A. tamarense (Group III) were present at nearly all stations but in low abundance. Alexandrium minutum and A. tamarense (Group II) cells were not detected in any of the samples, thereby arguing for their absence from the specific North Sea region, at least at the time of the survey. The sympatric occurrence of A. tamarense Group I and Group III gives further support to the hypothesis that the groups/ribotypes of the A. tamarense species complex are cryptic species rather than variants belonging to the same species.
Coverage:
Median Latitude: 57.078000 * Median Longitude: -2.048000 * South-bound Latitude: 56.090000 * West-bound Longitude: -3.000000 * North-bound Latitude: 57.840000 * East-bound Longitude: -1.690000
Date/Time Start: 2004-06-01T00:00:00 * Date/Time End: 2004-06-01T00:00:00
Minimum DEPTH, water: m * Maximum DEPTH, water: 20 m
Event(s):
HE209_S10 (S10) * Latitude: 57.840000 * Longitude: -3.000000 * Date/Time: 2004-06-01T00:00:00 * Location: North Sea * Campaign: HE209 * Basis: Heincke * Method/Device: CTD/Rosette (CTD-RO)
HE209_S11 (S11) * Latitude: 57.810000 * Longitude: -2.120000 * Date/Time: 2004-06-01T00:00:00 * Location: North Sea * Campaign: HE209 * Basis: Heincke * Method/Device: CTD/Rosette (CTD-RO)
HE209_S12 (S12) * Latitude: 57.420000 * Longitude: -1.690000 * Date/Time: 2004-06-01T00:00:00 * Location: North Sea * Campaign: HE209 * Basis: Heincke * Method/Device: CTD/Rosette (CTD-RO)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEvent
2Date/Time of eventDate/Time
3Latitude of eventLatitude
4Longitude of eventLongitude
5Sample code/labelSample labelJohn, Uwe
6DEPTH, waterDepth watermGeocode
7Alexandrium tamarenseA. tamarense#/lJohn, UweReal-time quantitative polymerase chain reaction (qPCR)Group I/North American (NA)
8Alexandrium tamarenseA. tamarense#/lJohn, UweReal-time quantitative polymerase chain reaction (qPCR)Group III/Western European (WE)
9Alexandrium tamutumA. tamutum#/lJohn, UweReal-time quantitative polymerase chain reaction (qPCR)
10Alexandrium spp.Alexandrium spp.#/lJohn, UweReal-time quantitative polymerase chain reaction (qPCR)
11Alexandrium spp.Alexandrium spp.#/lJohn, UweQuantitative phytoplankton method (Utermöhl, 1958)
12Paralytic shellfish poisoning toxinsPSP toxinspmol/lJohn, UweModified liquid chromatography with fluorescence detection (Yu et al 1998)
Size:
404 data points

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