Citation:

j.1472-4669.2011.00281.x

Name: Relative abundance of prokaryotes in bacterial mat covered cold seep sediments of the Nile Deep Sea Fan
Published: 2011
License: https://creativecommons.org/licenses/by/3.0/

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Project(s):

Hotspot Ecosystem Research on the Margins of European Seas (HERMES)

Funding:

Sixth Framework Programme (FP6), grant/award no. 511234 : Hotspot Ecosystem Research on the Margins of European Seas

Coverage:

Median Latitude: 32.423223 * Median Longitude: 31.255873 * South-bound Latitude: 32.367690 * West-bound Longitude: 30.352170 * North-bound Latitude: 32.533150 * East-bound Longitude: 31.711030

Date/Time Start: 2006-10-25T01:01:00 * Date/Time End: 2006-10-29T21:39:00

Minimum DEPTH, sediment/rock: 0.005 m * Maximum DEPTH, sediment/rock: 0.150 m

Event(s):

M70/2a_760_PUC-40 (760-40) * Latitude: 32.368830 * Longitude: 31.711030 * Date/Time: 2006-10-25T01:01:00 * Elevation: -1122.0 m * Location: Amon Mud Volcano * Campaign: (BIONIL) * Basis: * Method/Device: Push corer (PUC) * Comment: PUC-40

M70/2a_765_PUC-49 (765-49) * Latitude: 32.367690 * Longitude: 31.704420 * Date/Time: 2006-10-26T18:18:00 * Elevation: -1158.0 m * Location: Amon Mud Volcano * Campaign: (BIONIL) * Basis: * Method/Device: Push corer (PUC) * Comment: PUC-49

M70/2a_773_PUC-38 (773-38) * Latitude: 32.533150 * Longitude: 30.352170 * Date/Time: 2006-10-29T21:39:00 * Elevation: -1698.0 m * Location: Pockmark area * Campaign: (BIONIL) * Basis: * Method/Device: Push corer (PUC) * Comment: PC 38 put in

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Event label	Event
2	Latitude of event	Latitude
3	Longitude of event	Longitude
4	DEPTH, sediment/rock	Depth sed	m			Geocode
5	Prokaryotes, abundance as single cells	Prok cell abund	10⁹ #/cm³	Grünke, Stefanie	Fluorescence determination of DAPI stain
6	Bacteria, targed with EUB338(I-III) oligonucleotide FISH-probe	EUB338 I-III	%	Grünke, Stefanie	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)	Hybridization conditions: formamide concentration = 30%; hybridization temperature = 46°C; washing temperature = 46°C
7	Archaea, targed with ARCH915 oligonucleotide FISH-probe	ARCH915	%	Grünke, Stefanie	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)	Hybridization conditions: formamide concentration = 30%; hybridization temperature = 46°C; washing temperature = 46°C
8	Anaerobic methanotrophic archaea-1, targeted with ANME-1-350 oligonucleotide FISH-probe	ANME-1-350	%	Grünke, Stefanie	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)	Hybridization conditions: formamide concentration = 40%; hybridization temperature = 46°C; washing temperature = 46°C
9	Anaerobic methanotrophic archaea-2a, targeted with ANME-2a-647 oligonucleotide FISH-probe	ANME-2a-647	%	Grünke, Stefanie	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)	Hybridization conditions: formamide concentration = 55%; hybridization temperature = 46°C; washing temperature = 46°C
10	Anaerobic methanotrophic archaea-3, targeted with ANME-3-1249 oligonucleotide FISH-probe	ANME-3-1249	%	Grünke, Stefanie	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)	Hybridization conditions: formamide concentration = 40%; hybridization temperature = 46°C; washing temperature = 46°C
11	Desulfusarcina/Desulfococcus, targeted with DSS658 oligonucleotide FISH-probe	DSS658	%	Grünke, Stefanie	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)	Hybridization conditions: formamide concentration = 60%; hybridization temperature = 46°C; washing temperature = 46°C
12	Acrobacter spp., targeted with ARC94 oligonucleotide FISH-probe	ARC94	%	Grünke, Stefanie	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)	Hybridization conditions: formamide concentration = 20%; hybridization temperature = 46°C; washing temperature = 48°C

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Size:

143 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Event	2 Latitude	3 Longitude	4 Depth sed [m]	5 Prok cell abund [10⁹ #/cm³]	6 EUB338 I-III [%]	7 ARCH915 [%]	8 ANME-1-350 [%]	9 ANME-2a-647 [%]	10 ANME-3-1249 [%]	11 DSS658 [%]	12 ARC94 [%]
M70/2a_773_PUC-38	32.53315	30.35217	0.005	5.16	57.7	8.2	0.9	0.1	0.0	0.2	15.2
M70/2a_773_PUC-38	32.53315	30.35217	0.015	5.13	67.7	14.6	3.5	0.1	0.0	0.0	15.0
M70/2a_773_PUC-38	32.53315	30.35217	0.025	3.08	60.0	16.0	6.4	0.1	0.0	0.0	9.8
M70/2a_773_PUC-38	32.53315	30.35217	0.035	2.40	55.0	18.9	4.7	0.5	0.0	0.1	0.0
M70/2a_773_PUC-38	32.53315	30.35217	0.045	1.98	31.0	28.8	5.5	0.3	0.0	0.0	0.0
M70/2a_773_PUC-38	32.53315	30.35217	0.095	0.84	11.7	12.7	5.8	0.0	0.0	0.0	0.0
M70/2a_760_PUC-40	32.36883	31.71103	0.005	4.35	89.0	11.7	0.6	0.8	0.0	0.3	1.3
M70/2a_760_PUC-40	32.36883	31.71103	0.015	1.38	64.2		0.5	0.8	0.2	0.1	3.0
M70/2a_760_PUC-40	32.36883	31.71103	0.025	0.64	48.8	26.7	2.2	0.0	0.0	2.2	2.8
M70/2a_760_PUC-40	32.36883	31.71103	0.035	0.89	43.7	25.9	0.7	0.0	0.0	0.0	0.0
M70/2a_760_PUC-40	32.36883	31.71103	0.045	0.93	49.7	41.5	14.0	0.4	0.0	0.0	0.0
M70/2a_760_PUC-40	32.36883	31.71103	0.055	0.91	33.4	45.4	5.3	0.8	0.0	0.4	0.0
M70/2a_765_PUC-49	32.36769	31.70442	0.005	3.00	37.3	12.9	0.1	0.0	0.0	0.0	0.3
M70/2a_765_PUC-49	32.36769	31.70442	0.015	3.17	29.8	10.8	0.0	0.4	0.0	0.0	0.2
M70/2a_765_PUC-49	32.36769	31.70442	0.035	1.49	44.3	12.1	0.9	0.1	0.5	0.3	0.0
M70/2a_765_PUC-49	32.36769	31.70442	0.045	0.68	18.8	10.5	3.7	1.3	1.7	0.6	0.0
M70/2a_765_PUC-49	32.36769	31.70442	0.095	0.51	22.9	15.5	1.2	0.0	2.2	0.5	0.0
M70/2a_765_PUC-49	32.36769	31.70442	0.150	0.44	16.8	24.5	0.6	0.0	0.9	0.9	0.0