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Xie, Sitan; Lipp, Julius S; Wegener, Gunter; Ferdelman, Timothy G; Hinrichs, Kai-Uwe (2013): Degradation of [14C]GlcDGD in the marine sediment by monitoring radioactivity in the aqueous and gas phase using liquid scintillation counting (LSC) techniques. PANGAEA, https://doi.org/10.1594/PANGAEA.815450, Supplement to: Xie, S et al. (2013): Turnover of microbial lipids in the deep biosphere and growth of benthic archaeal populations. 110(15), 6010-6014, https://doi.org/10.1073/pnas.1218569110

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Abstract:
An experiment was conceived in which we monitored degradation of GlcDGD. Independent of the fate of the [14C]glucosyl headgroup after hydrolysis from the glycerol backbone, the 14C enters the aqueous or gas phase whereas the intact lipid is insoluble and remains in the sediment phase. Total degradation of GlcDGD then is obtained by combining the increase of radioactivity in the aqueous and gaseous phases. We chose two different sediment to perform this experiment. One is from microbially actie surface sediment sampled in February 2010 from the upper tidal flat of the German Wadden Sea near Wremen (53° 38' 0N, 8° 29' 30E). The other one is deep subsurface sediments recovered from northern Cascadia Margin during Integrated Ocean Drilling Program Expedition 311 [site U1326, 138.2 meters below seafloor (mbsf), in situ temperature 20 °C, water depth 1,828 m. We performed both alive and killed control experiments for comparison. Surface and subsurface sediment slurry were incubated in the dark at in situ temperature, 4 °C and 20 °C for 300 d, respectively. The sterilized slurry was stored at 20 °C. All incubations were carried out under N2 headspace to ensure anaerobic conditions. The sampling frequency was high during the first half-month, i.e., after 1, 2, 7, and 14 d; thereafter, the sediment slurry was sampled every 2 months. At each time point, samples were taken in triplicate for radioactivity measurements. After 300 d of incubation, no significant changes of radioactivity in the aqueous phase were detected. This may be the result of either the rapid turnover of released [14C] glucose or the relatively high limit of detection caused by the slight solubility (equivalent to 2% of initial radioactivity) of GlcDGD in water. Therefore, total degradation of GlcDGD in the dataset was calculated by combining radioactivity of DIC, CH4, and CO2, leading to a minimum estimate.
Coverage:
Median Latitude: 51.130316 * Median Longitude: -59.254586 * South-bound Latitude: 48.627300 * West-bound Longitude: -127.000840 * North-bound Latitude: 53.633333 * East-bound Longitude: 8.491667
Date/Time Start: 2011-02-21T00:00:00 * Date/Time End: 2011-12-20T00:00:00
Minimum DEPTH, sediment/rock: 0.0 m * Maximum DEPTH, sediment/rock: 138.2 m
Event(s):
311-U1326C * Latitude: 48.627300 * Longitude: -127.000840 * Elevation: -1828.0 m * Recovery: 54.39 m * Campaign: Exp311 (Cascadia Margin Gas Hydrates) * Basis: Joides Resolution * Method/Device: Drilling/drill rig (DRILL) * Comment: 13 cores; 85.7 m cored; 63.5 % recovered; 1 m drilled; 86.7 m penetrated
German_Wadden_Sea_2010 * Latitude: 53.633333 * Longitude: 8.491667 * Elevation: 0.0 m * Location: German Bight, North Sea * Method/Device: Multiple investigations (MULT) * Comment: sampled in February 2010
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEvent
2DEPTH, sediment/rockDepthmGeocode
3Sample, optional label/labor noLab noXie, Sitan
4DATE/TIMEDate/TimeGeocode
5Incubation durationInc durdaysXie, Sitan
6Temperature, technicalT tech°CXie, Sitan
7RadioactivityRadioactivity%Xie, SitanLiquid scintillation counter (TR-2900, Canberra-Packard)14C-DIC in aqueous
8RadioactivityRadioactivity%Xie, SitanLiquid scintillation counter (TR-2900, Canberra-Packard)14C-CO2
9RadioactivityRadioactivity%Xie, SitanLiquid scintillation counter (TR-2900, Canberra-Packard)14C-CH4
10RadioactivityRadioactivity%Xie, SitanLiquid scintillation counter (TR-2900, Canberra-Packard)Total degradation of GlcDGD
11CommentCommentXie, Sitan
Size:
640 data points

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