/* DATA DESCRIPTION: Citation: Garde, Eva; Frie, Anne K; Dunshea, Glenn; Hansen, Steen H; Kovacs, Kit Maureen; Lydersen, Christian (2010): (Table 2) Age determination of harp seals (Pagophilus groenlandicus) from the Barents Sea - comparison of methods. PANGAEA, https://doi.org/10.1594/PANGAEA.810152, Supplement to: Garde, E et al. (2010): Harp seal ageing techniques-teeth, aspartic acid racemization, and telomere sequence analysis. Journal of Mammalogy, 91(6), 1365-1374, https://doi.org/10.1644/10-MAMM-A-080.1 Abstract: Lower jaws (containing the teeth), eyes, and skin samples were collected from harp seals (Pagophilus groenlandicus) in the southeastern Barents Sea for the purpose of comparing age estimates obtained by 3 different methods, the traditional technique of counting growth layer groups (GLGs) in teeth and 2 novel approaches, aspartic acid racemization (AAR) in eye lens nuclei and telomere sequence analyses as a proxy for telomere length. A significant correlation between age estimates obtained using GLGs and AAR was found, whereas no correlation was found between GLGs and telomere length. An AAR rate (k Asp) of 0.00130/year ± 0.00005 SE and a D-enantiomer to L-enantiomer ratio at birth (D/L 0 value) of 0.01933 ± 0.00048 SE were estimated by regression of D/L ratios against GLG ages from 25 animals (12 selected teeth that had high readability and 13 known-aged animals). AAR could prove to be useful, particularly for ageing older animals in species such as harp seals where difficulties in counting GLGs tend to increase with age. Age estimation by telomere length did not show any correlation with GLG ages and is not recommended for harp seals. Project(s): International Polar Year (2007-2008) (IPY) (URI: https://www.ipy.org/) Coverage: MEDIAN LATITUDE: 69.300000 * MEDIAN LONGITUDE: 42.875000 * SOUTH-BOUND LATITUDE: 68.400000 * WEST-BOUND LONGITUDE: 40.167000 * NORTH-BOUND LATITUDE: 70.200000 * EAST-BOUND LONGITUDE: 45.583000 DATE/TIME START: 2006-04-09T00:00:00 * DATE/TIME END: 2006-04-24T00:00:00 Event(s): BarentsSea_seal * LATITUDE START: 68.400000 * LONGITUDE START: 40.167000 * LATITUDE END: 70.200000 * LONGITUDE END: 45.583000 * DATE/TIME START: 2006-04-09T00:00:00 * DATE/TIME END: 2006-04-24T00:00:00 * LOCATION: Barents Sea * METHOD/DEVICE: Biological sample (BIOS) Comment: Data extracted in the frame of a joint ICSTI/PANGAEA IPY effort, see http://doi.pangaea.de/10.1594/PANGAEA.150150 Parameter(s): Sample ID (Sample ID) * PI: Garde, Eva * COMMENT: harp seal no. Sex (Sex) * PI: Garde, Eva Aspartic acid D/L ratio (Asp D/L) * PI: Garde, Eva * COMMENT: corrected values Pagophilus groenlandicus, age [a] (P. groenlandicus age) * PI: Garde, Eva * METHOD/DEVICE: Ageing, aspartic acid racemization (AAR) in eye lens nuclei (URI: https://en.wikipedia.org/wiki/Amino_acid_dating) Pagophilus groenlandicus, standard deviation [±] (P. groenlandicus std dev) * PI: Garde, Eva * METHOD/DEVICE: Ageing, aspartic acid racemization (AAR) in eye lens nuclei (URI: https://en.wikipedia.org/wiki/Amino_acid_dating) * COMMENT: AAR age Pagophilus groenlandicus, age [a] (P. groenlandicus age) * PI: Garde, Eva * METHOD/DEVICE: Ageing, counting growth layer groups (GLG) in teeth (URI: https://doi.org/10.1126/science.112.2907.309-a) License: Creative Commons Attribution 3.0 Unported (CC-BY-3.0) (URI: https://creativecommons.org/licenses/by/3.0/) Size: 527 data points */ Sample ID (harp seal no.) Sex Asp D/L (corrected values) P. groenlandicus age [a] (Ageing, aspartic acid racemiz...) P. groenlandicus std dev [±] (AAR age, Ageing, aspartic aci...) P. groenlandicus age [a] (Ageing, counting growth layer...) 18 F 0.03029 8.4 1.3 10 19 F 0.02159 1.7 1.1 2 20 M 0.04838 22.4 1.7 16 21 M 0.03238 10.1 1.3 7 22 M 0.04711 21.4 1.7 16 23 F 0.01946 0.1 1.1 1 24 M 0.01807 -1.0 1.1 2 28 M 0.02370 3.4 1.2 5 35A M 0.05614 28.4 2.0 22 37 F 0.02289 2.7 1.1 1 39 F 0.02543 4.7 1.2 5 40 M 0.05114 24.5 1.8 22 41 F 0.05489 27.4 1.9 25 44 0.02229 2.3 1.1 4 46 M 0.02476 4.2 1.2 5 47 M 0.02323 3.0 1.1 5 49 M 0.02744 6.3 1.2 8 50 M 0.04848 22.5 1.7 16 51 F 0.05402 26.7 1.9 18 54 F 0.02497 4.3 1.2 5 55 M 0.02761 6.4 1.2 7 57 M 0.02516 4.5 1.2 6 58 M 0.03597 12.8 1.4 12 59 F 0.05222 25.3 1.9 15 60 F 0.05225 25.4 1.9 20 61 M 0.05432 27.0 1.9 16 63 M 0.03771 14.2 1.5 13 64 F 0.05367 26.5 1.9 19 65 M 0.05444 27.0 1.9 19 66 M 0.05554 27.9 1.9 19 67 F 0.05748 29.4 2.0 23 68 M 0.03270 10.3 1.4 12 70 M 0.02859 7.1 1.3 9 71 F 0.06195 32.8 2.1 25 72 F 0.01844 -0.7 1.1 1 73 M 0.04309 18.3 1.6 18 74 M 0.04650 20.9 1.7 18 75 M 0.02028 0.7 1.1 3 76 M 0.04569 20.3 1.7 14 79 F 0.05778 29.6 2.0 14 80 M 0.02843 7.0 1.3 8 81 F 0.02418 3.7 1.2 6 84 F 0.05760 29.5 2.0 24 85 M 0.03380 11.1 1.4 11 86 M 0.05069 24.2 1.8 20 87 M 0.04587 20.4 1.7 18 88 F 0.02519 4.5 1.2 6 91 F 0.02418 3.7 1.2 6 92 M 0.04195 17.4 1.6 25 94 F 0.02264 2.5 1.1 5 95 M 0.05134 24.7 1.8 12 96 F 0.04971 23.4 1.8 16 97 M 0.02000 0.5 1.1 2 98 F? 0.04835 22.4 1.7 20 99 M 0.03810 14.5 1.5 13 100 M 0.04679 21.2 1.7 20 101 M 0.03188 9.7 1.3 9 104 M 0.04243 17.8 1.6 21 105 F 0.01899 -0.3 1.1 1 106 M 0.02068 1.0 1.1 2 107 M 0.02351 3.2 1.2 2 108 F 0.05358 26.4 1.9 18 109 M 0.03242 10.1 1.3 11 111 M 0.04416 19.1 1.6 18 112 M 0.03424 11.5 1.4 14 113 M 0.02172 1.8 1.1 3 114 M 0.03387 11.2 1.4 14 116 M 0.05297 25.9 1.9 18 117 M 0.02542 4.7 1.2 7 118 F 0.02396 3.6 1.2 6 119 M 0.04874 22.7 1.8 17 120 M 0.02797 6.7 1.2 9 121 F 0.04934 23.1 1.8 16 123 F 0.03351 10.9 1.4 11 124 M 0.03122 9.2 1.3 9 125 F 0.04508 19.8 1.7 15 126 M 0.02518 4.5 1.2 7 127 M 0.02266 2.6 1.1 5 128 M 0.04796 22.1 1.7 16 138 F 0.02792 6.6 1.2 8 139 M 0.03240 10.1 1.3 10 140 M 0.04604 20.6 1.7 18 141 F 0.03053 8.6 1.3 9 142 M 0.03158 9.4 1.3 8 143 M 0.04957 23.3 1.8 24 144 M 0.04636 20.8 1.7 17 145 F 0.02322 3.0 1.1 6 146 M 0.03081 8.8 1.3 7