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Borrego-Acevedo, Rodney; Roelfsema, Christiaan M; Phinn, Stuart R; Grinham, Alistair (2013): Microphytobenthos composition in Heron Reef, Australia, by High Performance Liquid Chromatography (HPLC) (July 2012). PANGAEA,, Supplement to: Borrego-Acevedo, R et al. (2014): Predicting distribution of microphytobenthos abundance on a reef platform by combining in-situ underwater spectrometry and pigment analysis. Limnology and Oceanography, 5(5), 461-470,

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We quantified pigment biomarkers by high performance liquid chromatography (HPLC) to obtain a broad taxonomic classification of microphytobenthos (MPB) (i.e. identification of dominant taxa). Three replicate sediment cores were collected at 0, 50 and 100 m along transects 5-9 in Heron Reef lagoon (n=15) (Fig. 1). Transects 1-4 could not be processed because the means to have the samples analysed by HPLC were not available at the time of field data collection. Cores were stored frozen and scrapes taken from the top of each one and placed in cryovials immersed in dry ice. Samples were sent to the laboratory (CSIRO Marine and Atmospheric Research, Hobart, Australia) where pigments were extracted with 100% acetone during fifteen hours at 4°C after vortex mixing (30 seconds) and sonication (15 minutes). Samples were then centrifuged and filtered prior to the analysis of pigment composition with a Waters - Alliance HPLC system equipped with a photo-diode array detector. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) and a binary gradient system with an elevated column temperature following a modified version of the Van Heukelem and Thomas (2001) method. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Standards for HPLC system calibration were obtained from Sigma (USA) and DHI (Denmark).
Related to:
Van Heukelem, L; Thomas, C S (2001): Computer-assisted high-performance liquid chromatography method development with applications to the isolation and analysis of phytoplankton pigments. Journal of Chromatography A, 1, 31-49,
Median Latitude: -23.451121 * Median Longitude: 151.944467 * South-bound Latitude: -23.455170 * West-bound Longitude: 151.932100 * North-bound Latitude: -23.447160 * East-bound Longitude: 151.953780
Minimum DEPTH, sediment/rock: 1.5 m * Maximum DEPTH, sediment/rock: 3.0 m
Heron_Reef * Latitude Start: -23.432317 * Longitude Start: 151.898827 * Latitude End: -23.449153 * Longitude End: 151.924951 * Location: Heron Reef, Great Barrier Reef, Queensland
#NameShort NameUnitPrincipal InvestigatorMethodComment
1NumberNoBorrego-Acevedo, Rodney
2DEPTH, sediment/rockDepthmGeocode
5Distance, relativeDist relmBorrego-Acevedo, Rodney
6CommentCommentBorrego-Acevedo, Rodney
7MassMassgBorrego-Acevedo, Rodneysediment weight
8Fucoxanthin per unit sediment massFuco/sedµg/gBorrego-Acevedo, Rodney
9Peridinin per unit sediment massPerid/sedµg/gBorrego-Acevedo, Rodney
10Zeaxanthin per unit sediment massZea/sedµg/gBorrego-Acevedo, Rodney
11Chlorophyllide a per unit sediment massChlide a/sedµg/gBorrego-Acevedo, Rodney
120 data points

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