Petersen Jillian M 0000-0002-9852-3445 Zielinski Frank U Pape Thomas tpape@marum.de 0000-0002-6872-2144 Seifert Richard seifert@geowiss.uni-hamburg.de http://www.geowiss.uni-hamburg.de/i-bioge/start.html Moraru Cristina Amann Rudolf 0000-0002-0846-7372 Hourdez Stéphane Girguis Peter R 0000-0002-3599-8160 Wankel Scott D Barbe Valerie 0000-0002-8162-3343 Pelletier Eric 0000-0003-4228-1712 Fink Dennis Borowski Christian cborowsk@mpi-bremen.de 0000-0001-7921-3022 Bach Wolfgang wbach@uni-bremen.de https://www.marum.de/en/Wolfgang-Bach.html 0000-0002-3099-7142 Dubilier Nicole ndubilie@mpi-bremen.de http://www.marum.de/Nicole_Dubilier.html 0000-0002-9394-825X 2013 Hydrogen consumption by gills of Bathymodiolus spp. collected at the Comfortless cove and Lilliput hydrothermal vent fields in May 2006 dataset https://doi.org/10.1594/PANGAEA.808951 2013-03-16T21:52:56 https://doi.org/10.1594/PANGAEA.810465 The discovery of deep-sea hydrothermal vents in 1977 revolutionized our understanding of the energy sources that fuel primary productivity on Earth. Hydrothermal vent ecosystems are dominated by animals that live in symbiosis with chemosynthetic bacteria. So far, only two energy sources have been shown to power chemosynthetic symbioses: reduced sulphur compounds and methane. Using metagenome sequencing, single-gene fluorescence in situ hybridization, immunohistochemistry, shipboard incubations and in situ mass spectrometry, we show here that the symbionts of the hydrothermal vent mussel Bathymodiolus from the Mid-Atlantic Ridge use hydrogen to power primary production. In addition, we show that the symbionts of Bathymodiolus mussels from Pacific vents have hupL, the key gene for hydrogen oxidation. Furthermore, the symbionts of other vent animals such as the tubeworm Riftia pachyptila and the shrimp Rimicaris exoculata also have hupL. We propose that the ability to use hydrogen as an energy source is widespread in hydrothermal vent symbioses, particularly at sites where hydrogen is abundant. Koschinsky Andrea akoschinsky@constructor.university 0000-0002-9224-0663 Billings Andrew Devey Colin W cdevey@geomar.de 0000-0002-0930-7274 Dubilier Nicole ndubilie@mpi-bremen.de http://www.marum.de/Nicole_Dubilier.html 0000-0002-9394-825X Duester Alan Edge David Garbe-Schönberg Dieter dieter.garbe-schoenberg@ifg.uni-kiel.de 0000-0001-9006-9463 German Christopher R cgerman@whoi.edu 0000-0002-3417-6413 Giere Olav olav.giere@zoologie.uni-hamburg.de Keir Robin rkeir@geomar.de Lackschewitz Klas Sven klackschewitz@geomar.de Mai Anh Hoang Marbler Herwig h.marbler@jacobs-university.de Mawick Jule Melchert Bernd bmelchert@geomar.de Mertens Christian cmertens@physik.uni-bremen.de http://www.ocean.uni-bremen.de/mitarbeiter/cmertens/index_cm.html 0000-0002-7602-4194 Ochsenhirt Wolf-Thilo Peters Marc Sander Sylvia Schmale Oliver Schmidt Werner Seifert Richard seifert@geowiss.uni-hamburg.de http://www.geowiss.uni-hamburg.de/i-bioge/start.html Seiter Christian Stöber Uwe 0000-0002-2955-2453 Suck Inken inkens@geomar.de Walter Maren maren.walter@uni-bremen.de http://www.ocean.uni-bremen.de/mitarbeiter/mwalter/index_mw_eng.html Weber Stefan stefan.weber@uni-hamburg.de Yoerger Dana Zarrouk Marcel mschroeder@marum.de Zielinski Frank frank.zielinski@ufz.de 2006 Mid-Atlantic Expedition 2006, Cruise No. 68, Leg 1, Fluid geochemistry, biology and geological setting of hydrothermal systems at the southern MAR (4°S - 10°S) (MAR-SÜD III), 27 April - 2 June 2006, Bridgetown (Barbados) - Recife (Brazil) report METEOR-Berichte, Leitstelle Meteor, Institut für Meereskunde der Universität Hamburg 06 https://doi.org/10.2312/cr_m68-1 189 pp Koschinsky Andrea akoschinsky@constructor.university 0000-0002-9224-0663 Billings Andrew Devey Colin W cdevey@geomar.de 0000-0002-0930-7274 Dubilier Nicole ndubilie@mpi-bremen.de http://www.marum.de/Nicole_Dubilier.html 0000-0002-9394-825X Duester Alan Edge David Garbe-Schönberg Dieter dieter.garbe-schoenberg@ifg.uni-kiel.de 0000-0001-9006-9463 German Christopher R cgerman@whoi.edu 0000-0002-3417-6413 Giere Olav olav.giere@zoologie.uni-hamburg.de Keir Robin rkeir@geomar.de Lackschewitz Klas Sven klackschewitz@geomar.de Mai Anh Hoang Marbler Herwig h.marbler@jacobs-university.de Mawick Jule Melchert Bernd bmelchert@geomar.de Mertens Christian cmertens@physik.uni-bremen.de http://www.ocean.uni-bremen.de/mitarbeiter/cmertens/index_cm.html 0000-0002-7602-4194 Peters Michael Sander Sylvia Schmale Oliver Schmidt Werner Seifert Richard seifert@geowiss.uni-hamburg.de http://www.geowiss.uni-hamburg.de/i-bioge/start.html Seiter Christian Stöber Uwe 0000-0002-2955-2453 Suck Inken inkens@geomar.de Walter Maren maren.walter@uni-bremen.de http://www.ocean.uni-bremen.de/mitarbeiter/mwalter/index_mw_eng.html Weber Stefan stefan.weber@uni-hamburg.de Yoerger Dana Zarrouk Marcel mschroeder@marum.de Zielinski Frank frank.zielinski@ufz.de 2006 Discovery of new hydrothermal vent fields on the Southern Mid-Atlantic Ridge (4°S - 10°S) during Cruise M68/1 report InterRidge News 15 hdl:10013/epic.41214.d001 9-15 Petersen Jillian M 0000-0002-9852-3445 Zielinski Frank U Pape Thomas tpape@marum.de 0000-0002-6872-2144 Seifert Richard seifert@geowiss.uni-hamburg.de http://www.geowiss.uni-hamburg.de/i-bioge/start.html Moraru Cristina Amann Rudolf 0000-0002-0846-7372 Hourdez Stéphane Girguis Peter R 0000-0002-3599-8160 Wankel Scott D Barbe Valerie 0000-0002-8162-3343 Pelletier Eric 0000-0003-4228-1712 Fink Dennis Borowski Christian cborowsk@mpi-bremen.de 0000-0001-7921-3022 Bach Wolfgang wbach@uni-bremen.de https://www.marum.de/en/Wolfgang-Bach.html 0000-0002-3099-7142 Dubilier Nicole ndubilie@mpi-bremen.de http://www.marum.de/Nicole_Dubilier.html 0000-0002-9394-825X 2011 Hydrogen is an energy source for hydrothermal vent symbioses journal article Nature 476 https://doi.org/10.1038/nature10325 176-180 Zielinski Frank frank.zielinski@ufz.de 2009 Geobiological coupling of hydrothermal vent fluids with endosymbiotic primary producers of bathymodiolus mussels from hydrothermal vents on the Mid-Atlantic Ridge dissertation University of Bremen, Germany urn:nbn:de:gbv:46-diss000115375 159 pp -13.209130000000016 -12.371500000000026 -9.55338 -4.80233 -12.791273750000016 -7.176583750000001 2006-05-14T11:06:00 2006-05-27T13:45:00 -2993.0 -1475.0 not specified DERIDGE From Mantle to Ocean: Energy-, Material- and Life-cycles at Spreading Axes https://www.geomar.de/forschen/fb4/fb4-muhs/projekte/spp1144/ Research Center for Marine Geosciences at Christian Albrechts University, Kiel Geomar http://www.geomar.de/en/ German Research Foundation DFG http://www.dfg.de/en/ https://doi.org/10.13039/501100001659 5471797 From Mantle to Ocean: Energy-, Material- and Life-cycles at Spreading Axes https://gepris.dfg.de/project/5471797 M68/1-20-ROV -4.80283 -12.3725 -2993.0 2006-05-14T11:06:00 -4.80283 -12.3715 M68/1 M68/1 MARSUED3 MARSUED3 Bridgetown, Barbados 2006-04-27T00:00:00 2006-04-27 Recife, Brazil 2006-06-02T00:00:00 2006-06-02 Meteor (1986) https://en.wikipedia.org/wiki/RV_Meteor_(1986) DBBH 8411279 Koschinsky, Andrea Koschinsky, Andrea doi:10.2312/cr_m68-1 20060127 hdl:10013/epic.44784.d020 hdl:10013/epic.44803.d085 Meteor (1986) https://en.wikipedia.org/wiki/RV_Meteor_(1986) DBBH 8411279 Remote operated vehicle ROV Twin Peaks M68/1-24-ROV -4.80233 -12.382 -2993.0 2006-05-15T15:06:00 -4.803 -12.3715 -2991.0 2006-05-16T01:32:00 M68/1 M68/1 MARSUED3 MARSUED3 Bridgetown, Barbados 2006-04-27T00:00:00 2006-04-27 Recife, Brazil 2006-06-02T00:00:00 2006-06-02 Meteor (1986) https://en.wikipedia.org/wiki/RV_Meteor_(1986) DBBH 8411279 Koschinsky, Andrea Koschinsky, Andrea doi:10.2312/cr_m68-1 20060127 hdl:10013/epic.44784.d020 hdl:10013/epic.44803.d085 Meteor (1986) https://en.wikipedia.org/wiki/RV_Meteor_(1986) DBBH 8411279 Remote operated vehicle ROV Turtle Pits area M68/1-39-ROV -9.55338 -13.20856 -1475.0 2006-05-18T19:24:00 -9.54863 -13.20913 -1494.0 2006-05-19T00:01:00 M68/1 M68/1 MARSUED3 MARSUED3 Bridgetown, Barbados 2006-04-27T00:00:00 2006-04-27 Recife, Brazil 2006-06-02T00:00:00 2006-06-02 Meteor (1986) https://en.wikipedia.org/wiki/RV_Meteor_(1986) DBBH 8411279 Koschinsky, Andrea Koschinsky, Andrea doi:10.2312/cr_m68-1 20060127 hdl:10013/epic.44784.d020 hdl:10013/epic.44803.d085 Meteor (1986) https://en.wikipedia.org/wiki/RV_Meteor_(1986) DBBH 8411279 Remote operated vehicle ROV S of Liliput, 2nd mussel bed M68/1-70-ROV -9.55017 -13.20633 -1495.0 2006-05-27T13:45:00 -9.5495 -13.20867 M68/1 M68/1 MARSUED3 MARSUED3 Bridgetown, Barbados 2006-04-27T00:00:00 2006-04-27 Recife, Brazil 2006-06-02T00:00:00 2006-06-02 Meteor (1986) https://en.wikipedia.org/wiki/RV_Meteor_(1986) DBBH 8411279 Koschinsky, Andrea Koschinsky, Andrea doi:10.2312/cr_m68-1 20060127 hdl:10013/epic.44784.d020 hdl:10013/epic.44803.d085 Meteor (1986) https://en.wikipedia.org/wiki/RV_Meteor_(1986) DBBH 8411279 Remote operated vehicle ROV Liliput area 1582 CC-BY-3.0 Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/ Hydrogen consumption incubations Sampling site: For on-board physiological experiments specimens belonging to undescribed Bathymodiolus species (B. spp.) were collected at the Comfortless Cove and Lilliput hydrothermal vent fields on the southern Mid-Atlantic Ridge. Mussels were sampled using nets (40 cm length, 20 cm diameter opening, mesh size 335 µm, Hydrobios, Kiel, Germany) handled by the manipulator arm of the remotely operated vehicles (ROVs) Quest 4000 m (MARUM, Bremen, Germany). Mussel dissection for shipboard incubations: Mussels were opened with a scalpel by cutting through the posterior and anterior adductor muscles. Viability was tested by prodding the foot with a dissection needle and only mussels whose foot contracted were used. The foot and both gills were separated from the remaining tissue using dissection scissors. Tissue pieces of 6 mm in diameter were cut out of the gill and the foot tissues using a steel hole-puncher. One tissue piece from each individual was frozen in liquid nitrogen for weight determination in the home laboratory. For negative controls, foot tissues were used which do not contain endosymbiotic bacteria. Experimental setup: For hydrogen partial pressures up to 1,000 ppm (in helium) glass serum vials (58 ml) were fully filled with chilled (4°C) sterile-filtered (0.22 µm) bottom seawater retrieved from 3,000 m water depth. One piece of gill tissue was placed in the vial, the vial closed with a gas-tight butyl rubber stopper, and crimped with aluminium seals. Control vials contained foot tissue or no tissue at all. 20 ml of hydrogen gas (100 ppm, 250 ppm or 1,000 ppm in helium, Linde) were injected through the rubber stopper using a gas-tight syringe (SGE Analytical Science and Hamilton) with a second syringe allowing pressure compensation through the outflow of the same volume of seawater. For hydrogen partial pressures above 1,000 ppm (in air) glass serum vials were processed as described above with the exception that the vials were filled with only 38 ml of seawater. Pure hydrogen gas (100%; Air Liquide) was then injected in the air headspace through the rubber stopper to the desired final partial pressure (1,000-3,000 ppm) using a gas-tight syringe. All vials were placed upside down to minimize possible gas exchange via the rubber stopper and incubated at 4°C on a slowly rotating table. At given time points a subsample was taken with a gas-tight syringe from the headspace with the pressure decrease compensated through the inflow of oxygen-saturated, chilled sterile-filtered seawater from a second syringe. Analysis of the headspace hydrogen content: The hydrogen concentration in the headspace was determined using a gas chromatograph (Thermo Trace GC ultra) equipped with a packed stainless steel column (Molecular Sieve 5A, carrier gas: He) and a pulse discharge detector (PDD). Recording and calculation of results was performed using a PC operated integration system (Thermo Chrom Card A/D). Analytical procedures were calibrated daily with commercial gas standards (Linde). Incubation conditions and rate calculations: The concentrations of dissolved hydrogen and oxygen were calculated from Crozier and Yamamoto (1974) [doi:10.1021/je60062a007] and Weiss (1970) [doi:10.1016/0011-7471(70)90037-9] under the assumption of Henry's law i.e. that the concentration of a dissolved gas in a solution is directly proportional to the partial pressure of that gas above the solution. The molar volume of an ideal gas (22.414 l mol-1) was used to convert between the partial pressure of a gas (ppm) and the amount of the gas (in moles) in the headspace. The rates of hydrogen removal from the headspace [in nmol h-1 (piece tissue)-1] were calculated for the first 60 minutes after addition of the electron source performing non-linear regression through the data points obtained during the complete incubation period of up to 60 hours. Exponential and hyperbolic regression described by the equations f=a*exp(b/(x+c)) and f=y0+(a*b)/(b+x) gave well-fitting regression curves (R2 = 0.920 and 0.921, respectively; standard error of estimate 6.434 and 6.400, respectively). The effect of methodological hydrogen removal from the headspace through subsampling was considered. The rates at which hydrogen disappeared from incubation vials containing only seawater but no tissues (chemical oxidation, hydrogen loss by diffusion) were subtracted from the tissue rates. Resulting rates were then normalized to gram wet weight (in nmol h-1 [g wet weight]-1). Event label Event Event Sample ID Sample ID Zielinski Frank frank.zielinski@ufz.de Sample ID Time in minutes Time min min Time T http://dbpedia.org/resource/Time Zielinski Frank frank.zielinski@ufz.de Time min [min] Hydrogen H2 ppmv hydrogen Zielinski Frank frank.zielinski@ufz.de headspace H2 [ppmv] Hydrogen concentration H2 nmol Amount of Substance N http://dbpedia.org/resource/Amount_of_substance Zielinski Frank frank.zielinski@ufz.de dissolved H2 [nmol] Wet mass Wet m mg wet mass Mass M http://dbpedia.org/resource/Mass Zielinski Frank frank.zielinski@ufz.de Wet m [mg] Tissue piece, number of Tissue p # number Abundance or Count Zielinski Frank frank.zielinski@ufz.de Tissue p [#] Hydrogen consumption rate H2 cons rate nmol/h Catalytic Activity http://dbpedia.org/resource/Catalysis Zielinski Frank frank.zielinski@ufz.de per gill tissue piece H2 cons rate [nmol/h] Hydrogen consumption rate per weight H2 cons rate nmol/h/g Weight http://dbpedia.org/resource/Weight Specific Activity http://search.ebscohost.com/login.aspx?direct=true&db=nlebk&AN=98508&site=ehost-live&ebv=EB&ppid=pp_610 Zielinski Frank frank.zielinski@ufz.de normalized to wet weight H2 cons rate [nmol/h/g] Comment Comment Zielinski Frank frank.zielinski@ufz.de Comment citable deNBIchemical author10070 author10333 author148 author21730 author21933 author22511 author23871 author26179 author26521 author28857 author29326 author29589 author29735 author30038 author30375 author30476 author30670 author31088 author31090 author31095 author31116 author33037 author34648 author34899 author34903 author35157 author35158 author35159 author35200 author35202 author35208 author35209 author35210 author35211 author35227 author36453 author36655 author39220 author39221 author39222 author39223 author39224 author47801 author661 award112 basis2 campaign31082 campaign3391660 event2422777 event2422781 event2422805 event2579879 funder20472 geocode1599 geocode1600 geocode1601 geocode8128 inst20038 journal11658 journal16725 license101 method11520 param105457 param122890 param122891 param123142 param123680 param1570 param16065 param5058 param53104 pi28857 project4042 ref29189 ref34797 ref39232 ref39664 term2663481 term33871 term38563 term40811 term43762 term43783 term43892 term43975 term43989 term44025 term44032 term77654 term77796 topotype1