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PANGAEA.
Data Publisher for Earth & Environmental Science

Li, William K W (2013): Bacteria abundance measured on water bottle samples during HUDSON cruise HUD94/8. PANGAEA, https://doi.org/10.1594/PANGAEA.808266

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Related to:
Spear, Donald (2000): JGOFS Canada Data Sets 1989-1998. CD-ROM Version 1.0 Dec. 2000, Marine Environmental Data Service, Department of Fisheries and Oceans, Canada
Project(s):
Coverage:
Median Latitude: 55.131667 * Median Longitude: -51.731667 * South-bound Latitude: 46.800000 * West-bound Longitude: -53.860000 * North-bound Latitude: 60.360000 * East-bound Longitude: -48.500000
Date/Time Start: 1994-05-26T00:00:00 * Date/Time End: 1994-06-08T00:00:00
Minimum DEPTH, water: 0.0 m * Maximum DEPTH, water: 300.0 m
Event(s):
HUD94/8_BO2 * Latitude: 46.800000 * Longitude: -52.500000 * Date/Time: 1994-05-26T00:00:00 * Campaign: HUD94/8 * Basis: Hudson * Device: Bottle, Niskin (NIS)
HUD94/8_BO27 * Latitude: 60.360000 * Longitude: -48.500000 * Date/Time: 1994-05-30T00:00:00 * Campaign: HUD94/8 * Basis: Hudson * Device: Bottle, Niskin (NIS)
HUD94/8_BO3 * Latitude: 50.860000 * Longitude: -51.460000 * Date/Time: 1994-05-27T00:00:00 * Campaign: HUD94/8 * Basis: Hudson * Device: Bottle, Niskin (NIS)
Comment:
Samples for enumeration of bacterial abundance were drawn from Niskin bottles and immediately preserved in 20 mL glass scintillation vials with 0.2 µm filtered (Millex-GS, Millipore Corp.) formalin (2% final concentration). Preserved samples were stored dark in a refrigerator. Each sample was poured over triplicate polycarbonate membranes (0.2 µm black Nuclepore) underlain by pre-wetted backing filters (Sartorius #11306) which aid in distributing cells in an even manner on the Nuclepore surface. Cells were stained for 10 minutes with DAPI at a final concentration of 2 µg/ml and then drawn through the filters at a vacuum pressure of about 150 mm Hg. The filters were examined by epifluorescence microscopy (Leitz Orthoplan) under ultraviolet excitation from a mercury arc lamp (HBO 100W).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Event labelEvent
2Date/Time of eventDate/Time
3Latitude of eventLatitude
4Longitude of eventLongitude
5DEPTH, waterDepth watermGeocode
6BacteriaBact#/mlLi, BillCounting
Size:
181 data points

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