Li, William K W (2013): Bacteria and cyanobacteria abundance measured on water bottle samples during HUDSON cruise HUD93/2 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.808265
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Related to:
Spear, Donald (2000): JGOFS Canada Data Sets 1989-1998. CD-ROM Version 1.0 Dec. 2000, Marine Environmental Data Service, Department of Fisheries and Oceans, Canada
Project(s):
Joint Global Ocean Flux Study (JGOFS)
Coverage:
Median Latitude: 32.756250 * Median Longitude: -27.438750 * South-bound Latitude: 23.900000 * West-bound Longitude: -54.320000 * North-bound Latitude: 43.750000 * East-bound Longitude: -10.720000
Date/Time Start: 1993-05-20T00:00:00 * Date/Time End: 1993-06-06T00:00:00
Minimum DEPTH, water: 0.0 m * Maximum DEPTH, water: 200.0 m
Event(s):
HUD93/2_BO14 * Latitude: 31.490000 * Longitude: -10.720000 * Date/Time: 1993-05-20T00:00:00 * Campaign: HUD93/2 * Basis: Hudson * Method/Device: Bottle, Niskin (NIS)
Comment:
Bacterial counts
Samples for enumeration of bacterial abundance were drawn from Niskin bottles and immediately preserved in 20 mL glass scintillation vials with 0.2 µm filtered (Millex-GS, Millipore Corp.) formalin (2% final concentration). Preserved samples were stored dark in a refrigerator. Each sample was poured over triplicate polycarbonate membranes (0.2 µm black Nuclepore) underlain by pre-wetted backing filters (Sartorius #11306) which aid in distributing cells in an even manner on the Nuclepore surface. Cells were stained for 10 minutes with DAPI at a final concentration of 2 µg/ml and then drawn through the filters at a vacuum pressure of about 150 mm Hg. The filters were examined by epifluorescence microscopy (Leitz Orthoplan) under ultraviolet excitation from a mercury arc lamp (HBO 100W).
Cyanobacterial counts
Samples for enumeration of cyanobacterial abundance (20-100 ml) were freshly filtered in triplicate onto 0.2 µm black Nuclepore membranes which were underlain by backing filters. The membranes were stored refrigerated after having been covered in a drop of immersion oil. Cyanobacteria were enumerated by epifluorescence microscopy under green excitation (Leitz block N2).
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | ||||
| 2 | Date/Time of event | Date/Time | ||||
| 3 | Latitude of event | Latitude | ||||
| 4 | Longitude of event | Longitude | ||||
| 5 | DEPTH, water | Depth water | m | Geocode | ||
| 6 | Bacteria | Bact | #/ml | Li, Bill | Counting | |
| 7 | Cyanobacteria | Cyanobact | #/ml | Li, Bill | Counting |
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Size:
326 data points