/* DATA DESCRIPTION:
Citation:	Wittmann, Astrid C (2012): Temperature dependent growth and inorganic ion composition of haemolymph of juvenile Sepia officinalis, 2006 [dataset publication series]. Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven, PANGAEA, https://doi.org/10.1594/PANGAEA.788860
Abstract:	B Body wet weight and mantle length of juvenile Sepia officinalis were monitored over a peroid of five weeks. The animals had hatched in our aquarium system in Bremerhaven, Germany at 16°C and were descendants of individuals collected in the Oosterschelde estuary, Netherlands. Animals were kept in natural sea water at 10 or 17°C and fed small live shrimp (Palaemonetes varians) ad libitum daily. At the end of the experiment some animals kept at 17°C were sacrificed using ethanol. Haemolymph was withdrawn from the head vein using syringe and needle. Haemolymph samples were stored at -20°C until Na+, Cl-, K+, Mg2+, Ca2+ and SO42- concentrations were determined using ion chromatography. Mean body weight more that tripled at 17°C during the investigation period, while growth was impared by exposue to 10°C. Haemolymph ion concentrations were similar to those in sea water, except for sulphate. The concentration of this ion in the haemolymph was more that ten times lower than in sea water.
Coverage:	DATE/TIME START: 2006-11-08T00:00:00 * DATE/TIME END: 2006-12-20T00:00:00
Event(s):	Sepia_officinalis_2006 * METHOD/DEVICE: Experiment (EXP) * COMMENT: The animals had hatched in the aquarium system in the Alfred Wegener Institute, Bremerhaven, Germany at 16°C and were descendants of individuals collected in the Oosterschelde estuary, Netherlands.
License:	Creative Commons Attribution 3.0 Unported (CC-BY-3.0) (URI: https://creativecommons.org/licenses/by/3.0/)
Size:	3 datasets
*/
/* TABULAR SUMMARY OF DATASETS LISTED IN THIS COLLECTION: */
Filename	Citation of child dataset
Sepia_officinalis_2006_inorganic_comp.tab	Wittmann, Astrid C (2012): Inorganic ion composition of haemolymph of juvenile Sepia officinalis after growth experiments. https://doi.org/10.1594/PANGAEA.788859
Sepia_officinalis_2006_sea_water.tab	Wittmann, Astrid C (2012): Ion composition of natural sea water during growth experiments of Sepia officinalis. https://doi.org/10.1594/PANGAEA.788857
Sepia_officinalis_2006_weight_length.tab	Wittmann, Astrid C (2012): Body weight and mantle length of Sepia officinalis during growth experiments. https://doi.org/10.1594/PANGAEA.788858
