Wittmann, Astrid C; Pörtner, Hans-Otto; Sartoris, Franz-Josef (2012): Haemolymph inorganic ion composition, physiology and behaviour dependent on temperature and magnesium in adult Paralomis granulosa (Decapoda, Anomura, Lithodidae). PANGAEA, https://doi.org/10.1594/PANGAEA.788856, Supplement to: Wittmann, AC et al. (2012): A role for oxygen delivery and extracellular magnesium in limiting cold tolerance of the Sub-Antarctic stone crab Paralomis granulosa? Physiological and Biochemical Zoology, 85(3), 285-298, https://doi.org/10.1086/665328
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A low capacity for regulation of extracellular Mg2+ has been proposed to exclude reptant marine decapod crustaceans from temperatures below 0°C and thus to exclude them from the high Antarctic. To test this hypothesis and to elaborate the underlying mechanisms in the most cold-tolerant reptant decapod family of the sub-Antarctic, the Lithodidae, thermal tolerance was determined in the crab Paralomis granulosa (Decapoda, Anomura, Lithodidae) using an acute stepwise temperature protocol (-1°, 1°, 4°, 7°, 10°, and 13°C). Arterial and venous oxygen partial pressures (Po2) in hemolymph, heartbeat and ventilation beat frequencies, and hemolymph cation composition were measured at rest and after a forced activity (righting) trial. Scopes for heartbeat and ventilation beat frequencies and intermittent heartbeat and scaphognathite beat rates at rest were evaluated. Hemolymph [Mg2+] was experimentally reduced from 30 mmol/L to a level naturally observed in Antarctic caridean shrimps (12 mmol/L) to investigate whether the animals remain more active and tolerant to cold (-1°, 1°, and 4°C). In natural seawater, righting speed was significantly slower at -1° and 13°C, compared with acclimation temperature (4°C). Arterial and venous hemolymph Po2 increased in response to cooling even though heartbeat and ventilation beat frequencies as well as scopes decreased. At rest, ionic composition of the hemolymph was not affected by temperature. Activity induced a significant increase in hemolymph [K+] at -1° and 1°C. Reduction of hemolymph [Mg2+] did not result in an increase in activity, an increase in heartbeat and ventilation beat frequencies, or a shift in thermal tolerance to lower temperatures. In conclusion, oxygen delivery in this cold-water crustacean was not acutely limiting cold tolerance, and animals may have been constrained more by their functional capacity and motility. In contrast to earlier findings in temperate and subpolar brachyuran crabs, these constraints remained insensitive to changing Mg2+ levels.
Latitude: -53.160000 * Longitude: -70.930000
Punta_Arenas_2008b * Latitude: -53.160000 * Longitude: -70.930000 * Method/Device: Experiment (EXP) * Comment: Male specimens of Paralomis granulosa (fresh weight, 500 ± 63 g; carapace length, 9.6 ± 0.2 cm) were obtained from local fishermen in Punta Arenas, Chile, in April 2008. The animals were transported to the Alfred Wegener Institute for Polar and Marine Research, Bremerhaven, Germany, on board RV Polarstern (ANT-XXIV/4) and thereafter were kept in a recirculated aquarium system at 4°C in natural seawater (NSW) of 32.5 per mil salinity and an artificial 12 : 12 h light : dark cycle for approximately 1 yr.
Datasets listed in this publication series
- Wittmann, AC; Pörtner, H-O; Sartoris, F-J (2012): (Table 1) Cation composition of incubation media and hemolymph of Paralomis granulosa. https://doi.org/10.1594/PANGAEA.788781
- Wittmann, AC; Pörtner, H-O; Sartoris, F-J (2012): (Fig. 2, 3, 5) Arterial and venous hemolymph oxygen partial pressures, heart and ventilation beat frequencies, and hemolymph K+ concentration of Paralomis granulosa. https://doi.org/10.1594/PANGAEA.788783
- Wittmann, AC; Pörtner, H-O; Sartoris, F-J (2012): (Fig. 1 & 4) Mean time-to-right, pauses in heart beat and ventilation and scopes for heart and ventilation rate of Paralomis granulosa. https://doi.org/10.1594/PANGAEA.788782