Citation:

bio.2011036

Name: Seawater carbonate chemistry and expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera during experiments, 2012
Published: 2012-04-21
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Acropora digitifera; Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Cnidaria; Coast and continental shelf; Gene expression (incl. proteomics); Laboratory experiment; North Pacific; Single species; Temperate

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Abstract:

Ocean acidification is an ongoing threat for marine organisms due to the increasing atmospheric CO2 concentration. Seawater acidification has a serious impact on physiologic processes in marine organisms at all life stages. On the other hand, potential tolerance to external pH changes has been reported in coral larvae. Information about the possible mechanisms underlying such tolerance responses, however, is scarce. In the present study, we examined the effects of acidified seawater on the larvae of Acropora digitifera at the molecular level. We targeted two heat shock proteins, Hsp70 and Hsp90, and a heat shock transcription factor, Hsf1, because of their importance in stress responses and in early life developmental stages. Coral larvae were maintained under the ambient and elevated CO2 conditions that are expected to occur within next 100 years, and then we evaluated the expression of hsps and hsf1 by quantitative real-time polymerase chain reaction (PCR). Expression levels of these molecules significantly differed among target genes, but they did not change significantly between CO2conditions. These findings indicate that the expression of hsps is not changed due to external pH changes, and suggest that tolerance to acidified seawater in coral larvae may not be related to hsp expression.

Keyword(s):

Acropora digitifera; Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Cnidaria; Coast and continental shelf; Gene expression (incl. proteomics); Laboratory experiment; North Pacific; Single species; Temperate

Project(s):

European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)

European Project on Ocean Acidification (EPOCA)

Ocean Acidification International Coordination Centre (OA-ICC)

Funding:

Seventh Framework Programme (FP7), grant/award no. 211384 : European Project on Ocean Acidification

Sixth Framework Programme (FP6), grant/award no. 511106 : European network of excellence for Ocean Ecosystems Analysis

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Experimental treatment	Exp treat		Nakamura, Masoko
2	Experiment day	Exp day	day	Nakamura, Masoko
3	Salinity	Sal		Nakamura, Masoko	Salinometer (AS ONE, IS/Mill-E)
4	Temperature, water	Temp	°C	Nakamura, Masoko
5	pH	pH		Nakamura, Masoko	pH meter (Mettler Toledo, USA)	NBS scale
6	pH, standard error	pH std e	±	Nakamura, Masoko
7	Alkalinity, total	AT	µmol/kg	Nakamura, Masoko	Total alkalinity titration analyzer (Kimoto ATT-05, Japan)
8	Alkalinity, total, standard error	AT std e	±	Nakamura, Masoko
9	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Nakamura, Masoko	Calculated using CO2SYS
10	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error	pCO2water_SST_wet std e	±	Nakamura, Masoko
11	Bicarbonate ion	[HCO3]-	µmol/kg	Nakamura, Masoko	Calculated using CO2SYS
12	Bicarbonate ion, standard error	[HCO3]- std e	±	Nakamura, Masoko
13	Carbonate ion	[CO3]2-	µmol/kg	Nakamura, Masoko	Calculated using CO2SYS
14	Carbonate ion, standard error	[CO3]2- std e	±	Nakamura, Masoko
15	Aragonite saturation state	Omega Arg		Nakamura, Masoko	Calculated
16	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Nakamura, Masoko
17	Acropora digitifera, heat shock protein 70 expression	A. digitifera hsp70	#	Nakamura, Masoko	see reference(s)
18	Acropora digitifera, heat shock protein 70 expression, standard error	A. digitifera hsp70 std e	±	Nakamura, Masoko
19	Acropora digitifera, heat shock protein 90 expression	A. digitifera hsp90	#	Nakamura, Masoko	see reference(s)
20	Acropora digitifera, heat shock protein 90 expression, standard error	A. digitifera hsp90 std e	±	Nakamura, Masoko
21	Acropora digitifera, heat shock factor 1 expression	A. digitifera hsf1	#	Nakamura, Masoko	see reference(s)
22	Acropora digitifera, heat shock factor 1 expression, standard error	A. digitifera hsf1 std e	±	Nakamura, Masoko
23	Carbonate system computation flag	CSC flag		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
24	pH	pH		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)	Total scale
25	Carbon dioxide	CO2	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
26	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
27	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
28	Bicarbonate ion	[HCO3]-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
29	Carbonate ion	[CO3]2-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
30	Carbon, inorganic, dissolved	DIC	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
31	Aragonite saturation state	Omega Arg		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
32	Calcite saturation state	Omega Cal		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

256 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Exp treat	2 Exp day [day]	3 Sal (Salinometer (AS ONE, IS/Mill-E))	4 Temp [°C]	5 pH (NBS scale, pH meter (Mettler ...)	6 pH std e [±]	7 AT [µmol/kg] (Total alkalinity titration an...)	8 AT std e [±]	9 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	10 pCO2water_SST_wet std e [±]	11 [HCO3]- [µmol/kg] (Calculated using CO2SYS)	12 [HCO3]- std e [±]	13 [CO3]2- [µmol/kg] (Calculated using CO2SYS)	14 [CO3]2- std e [±]	15 Omega Arg (Calculated)	16 Omega Arg std dev [±]	17 A. digitifera hsp70 [#] (see reference(s))	18 A. digitifera hsp70 std e [±]	19 A. digitifera hsp90 [#] (see reference(s))	20 A. digitifera hsp90 std e [±]	21 A. digitifera hsf1 [#] (see reference(s))	22 A. digitifera hsf1 std e [±]	23 CSC flag (Calculated using seacarb afte...)	24 pH (Total scale, Calculated using...)	25 CO2 [µmol/kg] (Calculated using seacarb afte...)	26 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	27 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	28 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	29 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	30 DIC [µmol/kg] (Calculated using seacarb afte...)	31 Omega Arg (Calculated using seacarb afte...)	32 Omega Cal (Calculated using seacarb afte...)
Control (380 ppm)	1	35	27	8.13	0.01	2255.1	3.9	492.3	24.0	1776.9	16.6	194.1	6.2	3.0	0.1	0.0019	0.0012	0.5559	0.1496	0.0119	0.0037	26	7.99	12.26	455.11	453.71	1762.92	199.73	1974.90	3.20	4.82
1000 ppm	1	35	27	7.89	0.00	2255.9	4.2	925.6	10.8	1954.9	3.9	122.1	0.9	1.9	0.0	0.0063	0.0046	0.3776	0.1069	0.0151	0.0090	26	7.75	23.49	871.93	869.25	1944.22	126.79	2094.50	2.03	3.06
Control (380 ppm)	3	35	27	8.13	0.01	2255.1	3.9	492.3	24.0	1776.9	16.6	194.1	6.2	3.0	0.1	0.0024	0.0016	0.1239	0.0131	0.0072	0.0023	26	7.99	12.26	455.11	453.71	1762.92	199.73	1974.90	3.20	4.82
1000 ppm	3	35	27	7.89	0.00	2255.9	4.2	925.6	10.8	1954.9	3.9	122.1	0.9	1.9	0.0	0.0049	0.0040	0.1022	0.0120	0.0056	0.0015	26	7.75	23.49	871.93	869.25	1944.22	126.79	2094.50	2.03	3.06
Control (380 ppm)	5	35	27	8.13	0.01	2255.1	3.9	492.3	24.0	1776.9	16.6	194.1	6.2	3.0	0.1	0.0011	0.0006	0.1581	0.0865	0.0205	0.0112	26	7.99	12.26	455.11	453.71	1762.92	199.73	1974.90	3.20	4.82
1000 ppm	5	35	27	7.89	0.00	2255.9	4.2	925.6	10.8	1954.9	3.9	122.1	0.9	1.9	0.0	0.0003	0.0001	0.1014	0.0144	0.0045	0.0015	26	7.75	23.49	871.93	869.25	1944.22	126.79	2094.50	2.03	3.06
Control (380 ppm)	7	35	27	8.13	0.01	2255.1	3.9	492.3	24.0	1776.9	16.6	194.1	6.2	3.0	0.1	0.0015	0.0006	0.1207	0.0241	0.0112	0.0028	26	7.99	12.26	455.11	453.71	1762.92	199.73	1974.90	3.20	4.82
1000 ppm	7	35	27	7.89	0.00	2255.9	4.2	925.6	10.8	1954.9	3.9	122.1	0.9	1.9	0.0	0.0010	0.0004	0.0803	0.0162	0.0069	0.0028	26	7.75	23.49	871.93	869.25	1944.22	126.79	2094.50	2.03	3.06