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Langer, Gerald; Oetjen, Kerstin; Brenneis, Tina (2012): Seawater carbonate chemistry, nutrients, and Calcidiscus leptoporus (strain RCC1135) growth rate and calcification rate during experiments, 2012 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.778475, Supplement to: Langer, G et al. (2012): Calcification of Calcidiscus leptoporus under nitrogen and phosphorus limitation. Journal of Experimental Marine Biology and Ecology, 413, 131-137, https://doi.org/10.1016/j.jembe.2011.11.028

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Abstract:
The coccolithophore Calcidiscus leptoporus was grown in batch culture under nitrogen (N) as well as phosphorus (P) limitation. Growth rate, particulate inorganic carbon (PIC), particulate organic carbon (POC), particulate organic nitrogen (PON), and particulate organic phosphorus (POP) production were determined and coccolith morphology was analysed. While PON production decreased by 70% under N-limitation and POP production decreased by 65% under P-limitation, growth rate decreased by 33% under N- as well as P-limitation. POC as well as PIC production (calcification rate) increased by 27% relative to the control under P-limitation, and did not change under N-limitation. Coccolith morphology did not change in response to either P or N limitation. While these findings, supported by a literature survey, suggest that coccolith morphogenesis is not hampered by either P or N limitation, calcification rate might be. The latter conclusion is in apparent contradiction to our data. We discuss the reasons for this inference.
Keyword(s):
Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcidiscus leptoporus; Calcification/Dissolution; Chromista; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Macro-nutrients; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; South Atlantic
Project(s):
Biological Impacts of Ocean Acidification (BIOACID)
European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)
European Project on Ocean Acidification (EPOCA)
Mediterranean Sea Acidification in a Changing Climate (MedSeA)
Ocean Acidification International Coordination Centre (OA-ICC)
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Seventh Framework Programme (FP7), grant/award no. 265103: Mediterranean Sea Acidification in a Changing Climate
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 Experimental treatmentExp treatLanger, Gerald
2 Sample IDSample IDLanger, Gerald
3 SpeciesSpeciesLanger, Gerald
4 SalinitySalLanger, GeraldTetra Con 325 salinity and temperature probe
5 Temperature, waterTemp°CLanger, Gerald
6 Light:Dark cycleL:Dhh:hhLanger, GeraldMeasured
7 Radiation, photosynthetically activePARµmol/m2/sLanger, Gerald
8 Alkalinity, totalATµmol/kgLanger, GeraldAlkalinity, Gran titration (Gran, 1950)
9 Alkalinity, total, standard deviationAT std dev±Langer, Gerald
10 pHpHLanger, GeraldpH meter, WTW, 340iNBS scale
11 pH, standard deviationpH std dev±Langer, Gerald
12 Carbon, inorganic, dissolvedDICµmol/kgLanger, GeraldSeal QuAAtro SFA Analyzer, Seal Analytical, 800 TM
13 Carbon, inorganic, dissolved, standard deviationDIC std dev±Langer, Gerald
14 PhosphatePHSPHTµmol/kgLanger, GeraldAutoanalyzer (AA)
15 Phosphate, standard deviation[PO4]3- std dev±Langer, Gerald
16 Nitrate[NO3]-mmol/kgLanger, GeraldAutoanalyzer (AA)
17 Nitrate, standard deviationNO3 std dev±Langer, Gerald
18 Calcidiscus leptoporusC. leptoporus%Langer, GeraldNormal
19 Calcidiscus leptoporus, standard deviationC. leptoporus std dev±Langer, GeraldNormal
20 Calcidiscus leptoporusC. leptoporus%Langer, GeraldMalformed
21 Calcidiscus leptoporus, standard deviationC. leptoporus std dev±Langer, GeraldMalformed
22 Calcidiscus leptoporusC. leptoporus%Langer, GeraldIncomplete
23 Calcidiscus leptoporus, standard deviationC. leptoporus std dev±Langer, GeraldIncomplete
24 Calcidiscus leptoporusC. leptoporus%Langer, GeraldIncompete and malformed
25 Calcidiscus leptoporus, standard deviationC. leptoporus std dev±Langer, GeraldIncompete and malformed
26 Growth rateµ#/dayLanger, Gerald
27 Growth rate, standard deviationµ std dev±Langer, Gerald
28 Carbon, organic, particulate, per cellPOC/cellpg/#Langer, GeraldElement analyser CNS, EURO EA
29 Particulate organic carbon content per cell, standard deviationPOC cont/cell std dev±Langer, Gerald
30 Particulate organic carbon production per cellPOC prod/cellpg/#/dayLanger, GeraldCalculated, see reference(s)
31 Particulate organic carbon, production, standard deviationPOC prod std dev±Langer, Gerald
32 Carbon, inorganic, particulate, per cellPIC/cellpg/#Langer, GeraldCalculated, see reference(s)
33 Particulate inorganic carbon per cell, standard deviationPIC/cell std dev±Langer, Gerald
34 Calcification rate of carbon per cellCalc rate C/cellpg/#/hLanger, Gerald
35 Calcification rate, standard deviationCalc rate std dev±Langer, Gerald
36 Particulate organic phosphorus per cellPOP/cellpmol/#Langer, GeraldAutoanalyzer (AA)Original unit pg/cell, value devided by density
37 Particulate organic phosphorus per cell, standard deviationPOP/cell std dev±Langer, Gerald
38 Particulate organic phosphorus production per cellPOP prod/cellpmol/#/dayLanger, GeraldCalculated, see reference(s)Original unit pg/cell, value devided by density
39 Particulate organic phosphorus productionPOP prodµmol/l/dayLanger, GeraldOriginal unit pg/cell, value devided by density
40 Particulate organic nitrogen per cellPON/cellpg/#Langer, GeraldElement analyser CNS, EURO EA
41 Particulate organic nitrogen per cell, standard deviationPON/cell std dev±Langer, Gerald
42 Production of particulate organic nitrogenPON prodpg/#/dayLanger, GeraldCalculated, see reference(s)
43 Particulate organic nitrogen production, standard deviationPON prod std dev±Langer, Gerald
44 Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
45 pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)total scale
46 Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
47 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
48 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
49 Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
50 Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
51 Carbon, inorganic, dissolvedDICµmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
52 Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
53 Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
238 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
Exp treat		2 
Sample ID		3 
Species		4 
Sal
(Tetra Con 325 salinity and te...)		5 
Temp [°C]		6 
L:D [hh:hh]
(Measured)		7 
PAR [µmol/m2/s]		8 
AT [µmol/kg]
(Alkalinity, Gran titration (G...)		9 
AT std dev [±]		10 
pH
(NBS scale, pH meter, WTW, 340i)		11 
pH std dev [±]		12 
DIC [µmol/kg]
(Seal QuAAtro SFA Analyzer, Se...)		13 
DIC std dev [±]		14 
PHSPHT [µmol/kg]
(Autoanalyzer)		15 
[PO4]3- std dev [±]		16 
[NO3]- [mmol/kg]
(Autoanalyzer)		17 
NO3 std dev [±]		18 
C. leptoporus [%]
(Normal)		19 
C. leptoporus std dev [±]
(Normal)		20 
C. leptoporus [%]
(Malformed)		21 
C. leptoporus std dev [±]
(Malformed)		22 
C. leptoporus [%]
(Incomplete)		23 
C. leptoporus std dev [±]
(Incomplete)		24 
C. leptoporus [%]
(Incompete and malformed)		25 
C. leptoporus std dev [±]
(Incompete and malformed)		26 
µ [#/day]		27 
µ std dev [±]		28 
POC/cell [pg/#]
(Element analyser CNS, EURO EA)		29 
POC cont/cell std dev [±]		30 
POC prod/cell [pg/#/day]
(Calculated, see reference(s))		31 
POC prod std dev [±]		32 
PIC/cell [pg/#]
(Calculated, see reference(s))		33 
PIC/cell std dev [±]		34 
Calc rate C/cell [pg/#/h]		35 
Calc rate std dev [±]		36 
POP/cell [pmol/#]
(Original unit pg/cell, value ...)		37 
POP/cell std dev [±]		38 
POP prod/cell [pmol/#/day]
(Original unit pg/cell, value ...)		39 
POP prod [µmol/l/day]
(Original unit pg/cell, value ...)		40 
PON/cell [pg/#]
(Element analyser CNS, EURO EA)		41 
PON/cell std dev [±]		42 
PON prod [pg/#/day]
(Calculated, see reference(s))		43 
PON prod std dev [±]		44 
CSC flag
(Calculated using seacarb afte...)		45 
pH
(total scale, Calculated using...)		46 
CO2 [µmol/kg]
(Calculated using seacarb afte...)		47 
pCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		48 
fCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		49 
[HCO3]- [µmol/kg]
(Calculated using seacarb afte...)		50 
[CO3]2- [µmol/kg]
(Calculated using seacarb afte...)		51 
DIC [µmol/kg]
(Calculated using seacarb afte...)		52 
Omega Arg
(Calculated using seacarb afte...)		53 
Omega Cal
(Calculated using seacarb afte...)
ControlT0Calcidiscus leptoporus (strain RCC1135)321516:08400246237.940.0042210732.960.290.72740.0053267.8229.88787.18784.372215.64101.682347.21.582.47
ControlTfinCalcidiscus leptoporus (strain RCC1135)321516:084002208197.870.00920151831.450.150.68970.002943.832.7520.663.242.881.4232.635.270.540.0230.531.7916.530.5770.814.501.5970833330.0680.0100.0070.00110.451.385.650.62267.7531.91840.64837.642013.5678.642124.11.221.91
PO4limitedT0Calcidiscus leptoporus (strain RCC1135)321516:08400242927.980.012219440.250.010.72670.0034267.8626.62701.21698.712164.45108.932300.01.692.65
PO4limitedTfinCalcidiscus leptoporus (strain RCC1135)321516:084001956407.790.0191835230.000.000.71320.005747.201.3426.590.941.900.5524.410.870.360.0161.828.5122.632.46144.7112.392.1795833330.1600.0100.0020.00112.971.354.750.44267.6734.42906.82903.591807.1658.721900.30.911.43
NO3limitedT0Calcidiscus leptoporus (strain RCC1135)321516:08400246237.980.0082190834.980.250.00600.0001267.8626.97710.64708.112194.35110.482331.81.712.68
NO3limitedTfinCalcidiscus leptoporus (strain RCC1135)321516:084001896427.710.02634.420.230.00010.000148.251.4020.553.311.250.4229.954.240.360.0142.373.1615.240.95107.135.261.5979166670.0840.0200.0060.0014.791.001.720.32267.5940.641070.781066.971773.7547.911862.30.741.16