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Sinutok, Sutinee; Hill, Ross; Doblin, Martina A; Wuhrer, Richard; Ralph, Peter J (2011): Seawater carbonate chemistry, calcification rate, oxygen production, maximum quantum yield, symbiont density, chlorophyll concentration and crystal width of Halimeda macroloba, Halimeda cylindracea and Marginopora vertebralis during experiments, 2011 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.774792, Supplement to: Sinutok, S et al. (2011): Warmer more acidic conditions cause decreased productivity and calcification in subtropical coral reef sediment-dwelling calcifiers. Limnology and Oceanography, 56(4), 1200-1212, https://doi.org/10.4319/lo.2011.56.4.1200

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Abstract:
The effects of elevated CO2 and temperature on photosynthesis and calcification in the calcifying algae Halimeda macroloba and Halimeda cylindracea and the symbiont-bearing benthic foraminifera Marginopora vertebralis were investigated through exposure to a combination of four temperatures (28°C, 30°C, 32°C, and 34°C) and four CO2 levels (39, 61, 101, and 203 Pa; pH 8.1, 7.9, 7.7, and 7.4, respectively). Elevated CO2 caused a profound decline in photosynthetic efficiency (FV : FM), calcification, and growth in all species. After five weeks at 34°C under all CO2 levels, all species died. Chlorophyll (Chl) a and b concentration in Halimeda spp. significantly decreased in 203 Pa, 32°C and 34°C treatments, but Chl a and Chl c2 concentration in M. vertebralis was not affected by temperature alone, with significant declines in the 61, 101, and 203 Pa treatments at 28°C. Significant decreases in FV : FM in all species were found after 5 weeks of exposure to elevated CO2 (203 Pa in all temperature treatments) and temperature (32°C and 34°C in all pH treatments). The rate of oxygen production declined at 61, 101, and 203 Pa in all temperature treatments for all species. The elevated CO2 and temperature treatments greatly reduced calcification (growth and crystal size) in M. vertebralis and, to a lesser extent, in Halimeda spp. These findings indicate that 32°C and 101 Pa CO2, are the upper limits for survival of these species on Heron Island reef, and we conclude that these species will be highly vulnerable to the predicted future climate change scenarios of elevated temperature and ocean acidification.
Keyword(s):
Benthos; Biomass/Abundance/Elemental composition; Calcification/Dissolution; Chlorophyta; Chromista; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Foraminifera; Growth/Morphology; Heterotrophic prokaryotes; Laboratory experiment; Macroalgae; Marginopora vertebralis; Plantae; Primary production/Photosynthesis; Single species; South Pacific; Temperature; Tropical
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
IdentificationIDHill, Ross
Experimental treatmentExp treatHill, Ross
pHpHHill, RossMeasuredNBS scale
SalinitySalHill, Ross
Temperature, waterTemp°CHill, Ross
Alkalinity, totalATµmol/kgHill, RossAutotitrator (Mettler Toledo)
Alkalinity, total, standard errorAT std e±Hill, Ross
Carbon dioxide, partial pressurepCO2PaHill, RossCalculated using CO2SYS
Carbon dioxide, partial pressure, standard deviationpCO2 std dev±Hill, Ross
10 Carbon dioxideCO2µmol/kgHill, RossCalculated using CO2SYS
11 Carbon dioxide, standard errorCO2 std e±Hill, Ross
12 Carbonate ion[CO3]2-µmol/kgHill, RossCalculated using CO2SYS
13 Carbonate ion, standard error[CO3]2- std e±Hill, Ross
14 Bicarbonate ion[HCO3]-µmol/kgHill, RossCalculated using CO2SYS
15 Bicarbonate ion, standard error[HCO3]- std e±Hill, Ross
16 Carbon, inorganic, dissolvedDICµmol/kgHill, RossCalculated using CO2SYS
17 Carbon, inorganic, dissolved, standard errorDIC std e±Hill, Ross
18 Calcite saturation stateOmega CalHill, RossCalculated using CO2SYS
19 Calcite saturation state, standard deviationOmega Cal std dev±Hill, Ross
20 Aragonite saturation stateOmega ArgHill, RossCalculated using CO2SYS
21 Aragonite saturation state, standard deviationOmega Arg std dev±Hill, Ross
22 Calcification rateCalc ratemg/dayHill, RossBuoyant weighing technique according to Davies (1989)Halimeda macroloba
23 Calcification rate, standard errorCalc rate std e±Hill, RossHalimeda macroloba
24 Calcification rateCalc ratemg/dayHill, RossBuoyant weighing technique according to Davies (1989)Halimeda cylindracea
25 Calcification rate, standard errorCalc rate std e±Hill, RossHalimeda cylindracea
26 Calcification rateCalc ratemg/dayHill, RossBuoyant weighing technique according to Davies (1989)Marginopora vertebralis
27 Calcification rate, standard errorCalc rate std e±Hill, RossMarginopora vertebralis
28 Marginopora vertebralis, symbiont cell densityM. vertebralis symbiont#/mm2Hill, Ross
29 Marginopora vertebralis, symbiont density, standard errorM. vertebralis symbiont std e±Hill, Ross
30 Halimeda macroloba, chlorophyll aH. macroloba chl aµg/mm2Hill, RossSpectrophotometry
31 Halimeda macroloba, chlorophyll a, standard errorH. macroloba chl a std e±Hill, Ross
32 Halimeda macroloba, chlorophyll bH. macroloba chl bµg/mm2Hill, RossSpectrophotometry
33 Halimeda macroloba, chlorophyll b, standard errorH. macroloba chl b std e±Hill, Ross
34 Halimeda cylindracea, chlorophyll aH. cylindracea chl aµg/mm2Hill, RossSpectrophotometry
35 Halimeda cylindracea, chlorophyll a, standard errorH. cylindracea chl a std e±Hill, Ross
36 Halimeda cylindracea, chlorophyll bH. cylindracea chl bµg/mm2Hill, RossSpectrophotometry
37 Halimeda cylindracea, chlorophyll b, standard errorH. cylindracea chl b std e±Hill, Ross
38 Marginopora vertebralis, chlorophyll aM. vertebralis chl aµg/mm2Hill, RossSpectrophotometry
39 Marginopora vertebralis, chlorophyll a, standard errorM. vertebralis chl a std e±Hill, Ross
40 Marginopora vertebralis, chlorophyll c2M. vertebralis chl c2µg/mm2Hill, Ross
41 Marginopora vertebralis, chlorophyll c2, standard errorM. vertebralis chl c2 std e±Hill, Ross
42 Maximum photochemical quantum yield of photosystem IIFv/FmHill, Rosssee reference(s)Halimeda macroloba
43 Maximum photochemical quantum yield, standard errorFv/Fm std e±Hill, RossHalimeda macroloba
44 Maximum photochemical quantum yield of photosystem IIFv/FmHill, Rosssee reference(s)Halimeda cylindracea
45 Maximum photochemical quantum yield, standard errorFv/Fm std e±Hill, RossHalimeda cylindracea
46 Maximum photochemical quantum yield of photosystem IIFv/FmHill, Rosssee reference(s)Marginopora vertebralis
47 Maximum photochemical quantum yield, standard errorFv/Fm std e±Hill, RossMarginopora vertebralis
48 Oxygen production rateO2 prodµmol/lHill, RossFiber-optic oxygen microsensor PSt1 with MicroTX3 transmitter (Presens)Halimeda macroloba
49 Oxygen production rate, standard errorO2 prod std e±Hill, RossHalimeda macroloba
50 Oxygen production rateO2 prodµmol/lHill, RossFiber-optic oxygen microsensor PSt1 with MicroTX3 transmitter (Presens)Halimeda cylindracea
51 Oxygen production rate, standard errorO2 prod std e±Hill, RossHalimeda cylindracea
52 Oxygen production rateO2 prodµmol/lHill, RossFiber-optic oxygen microsensor PSt1 with MicroTX3 transmitter (Presens)Marginopora vertebralis
53 Oxygen production rate, standard errorO2 prod std e±Hill, RossMarginopora vertebralis
54 Halimeda macroloba, crystal widthH. macroloba crystalµmHill, RossScanning electron microscope (Zeiss Supra 55VP)
55 Halimeda macroloba, crystal width, standard errorH. macroloba crystal std e±Hill, Ross
56 Halimeda cylindracea, crystal widthH. cylindracea crystalµmHill, RossScanning electron microscope (Zeiss Supra 55VP)
57 Halimeda cylindracea, crystal width, standard errorH. cylindracea crystal std e±Hill, Ross
58 Marginopora vertebralis, crystal widthM. vertebralis crystalµmHill, RossScanning electron microscope (Zeiss Supra 55VP)
59 Marginopora vertebralis, crystal width, standard errorM. vertebralis crystal std e±Hill, Ross
60 Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
61 pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
62 Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
63 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
64 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
65 Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
66 Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
67 Carbon, inorganic, dissolvedDICµmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
68 Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
69 Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
3776 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


ID

Exp treat

pH
(NBS scale, Measured)

Sal

Temp [°C]

AT [µmol/kg]
(Autotitrator (Mettler Toledo))

AT std e [±]

pCO2 [Pa]
(Calculated using CO2SYS)

pCO2 std dev [±]
10 
CO2 [µmol/kg]
(Calculated using CO2SYS)
11 
CO2 std e [±]
12 
[CO3]2- [µmol/kg]
(Calculated using CO2SYS)
13 
[CO3]2- std e [±]
14 
[HCO3]- [µmol/kg]
(Calculated using CO2SYS)
15 
[HCO3]- std e [±]
16 
DIC [µmol/kg]
(Calculated using CO2SYS)
17 
DIC std e [±]
18 
Omega Cal
(Calculated using CO2SYS)
19 
Omega Cal std dev [±]
20 
Omega Arg
(Calculated using CO2SYS)
21 
Omega Arg std dev [±]
22 
Calc rate [mg/day]
(Halimeda macroloba, Buoyant w...)
23 
Calc rate std e [±]
(Halimeda macroloba)
24 
Calc rate [mg/day]
(Halimeda cylindracea, Buoyant...)
25 
Calc rate std e [±]
(Halimeda cylindracea)
26 
Calc rate [mg/day]
(Marginopora vertebralis, Buoy...)
27 
Calc rate std e [±]
(Marginopora vertebralis)
28 
M. vertebralis symbiont [#/mm2]
29 
M. vertebralis symbiont std e [±]
30 
H. macroloba chl a [µg/mm2]
(Spectrophotometry)
31 
H. macroloba chl a std e [±]
32 
H. macroloba chl b [µg/mm2]
(Spectrophotometry)
33 
H. macroloba chl b std e [±]
34 
H. cylindracea chl a [µg/mm2]
(Spectrophotometry)
35 
H. cylindracea chl a std e [±]
36 
H. cylindracea chl b [µg/mm2]
(Spectrophotometry)
37 
H. cylindracea chl b std e [±]
38 
M. vertebralis chl a [µg/mm2]
(Spectrophotometry)
39 
M. vertebralis chl a std e [±]
40 
M. vertebralis chl c2 [µg/mm2]
41 
M. vertebralis chl c2 std e [±]
42 
Fv/Fm
(Halimeda macroloba, see refer...)
43 
Fv/Fm std e [±]
(Halimeda macroloba)
44 
Fv/Fm
(Halimeda cylindracea, see ref...)
45 
Fv/Fm std e [±]
(Halimeda cylindracea)
46 
Fv/Fm
(Marginopora vertebralis, see ...)
47 
Fv/Fm std e [±]
(Marginopora vertebralis)
48 
O2 prod [µmol/l]
(Halimeda macroloba, Fiber-opt...)
49 
O2 prod std e [±]
(Halimeda macroloba)
50 
O2 prod [µmol/l]
(Halimeda cylindracea, Fiber-o...)
51 
O2 prod std e [±]
(Halimeda cylindracea)
52 
O2 prod [µmol/l]
(Marginopora vertebralis, Fibe...)
53 
O2 prod std e [±]
(Marginopora vertebralis)
54 
H. macroloba crystal [µm]
(Scanning electron microscope ...)
55 
H. macroloba crystal std e [±]
56 
H. cylindracea crystal [µm]
(Scanning electron microscope ...)
57 
H. cylindracea crystal std e [±]
58 
M. vertebralis crystal [µm]
(Scanning electron microscope ...)
59 
M. vertebralis crystal std e [±]
60 
CSC flag
(Calculated using seacarb afte...)
61 
pH
(Total scale, Calculated using...)
62 
CO2 [µmol/kg]
(Calculated using seacarb afte...)
63 
pCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)
64 
fCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)
65 
[HCO3]- [µmol/kg]
(Calculated using seacarb afte...)
66 
[CO3]2- [µmol/kg]
(Calculated using seacarb afte...)
67 
DIC [µmol/kg]
(Calculated using seacarb afte...)
68 
Omega Arg
(Calculated using seacarb afte...)
69 
Omega Cal
(Calculated using seacarb afte...)
T0T0500.45462.3775.1533.0340.6670.3910.4750.5790.1030.1163.7792.1591.2660.668
T0pH8.18.103328231418736.82.68128035163513519231606.030.954.040.610.4490.0420.3430.0910.2380.001267.9513.89522.97521.381848.44191.472053.803.124.71
T0pH8.18.103330264719036.92.781134030184214521901667.400.554.990.630.5030.0340.4710.0250.1960.030267.9515.40607.40605.592098.74230.962345.103.815.71
T0pH8.18.103332269518936.92.68136542183513822091628.020.735.440.600.4690.0540.4800.0170.1740.021267.9415.15625.14623.332111.54246.612373.304.126.13
T0pH8.18.103334239219032.22.67133835157913319241607.500.745.130.600.4200.0760.4800.0220.2620.027267.9412.94557.61556.031841.48228.082082.503.865.71
T0pH7.97.903328268516966.94.61622314214017023871703.340.103.340.090.4840.0610.3260.0340.1360.013267.7527.691042.491039.322325.22151.992504.902.483.74
T0pH7.97.903330262416865.14.51622403205817323141733.520.153.520.100.4830.0300.3880.0540.1700.023267.7526.181032.881029.822252.65156.472435.302.583.87
T0pH7.97.903332260717064.64.51522383204418022971823.490.123.490.100.5470.0220.3260.0360.1400.021267.7425.211040.631037.622218.72163.572407.502.734.07
T0pH7.97.903334230917056.34.61222354174317519907603.570.143.570.120.5010.0210.4620.0270.2060.020267.7421.61931.33928.701941.41151.782114.802.573.80
T0pH7.77.7033282532110108.04.72711481821728023471012.140.442.140.330.5300.0170.4510.0220.1950.022267.5543.511637.921632.952305.0395.062443.601.552.34
T0pH7.77.7033302565108110.04.7251160192185822370992.360.522.360.320.5300.0170.4510.0220.1950.022267.5542.771687.241682.242322.70101.842467.301.682.52
T0pH7.77.7033322427110104.04.82411621820428322281102.410.492.410.340.5300.0170.4510.0220.1950.022267.5439.301622.091617.402183.29101.612324.201.702.53
T0pH7.77.7033342691109115.04.8251191202244802460952.900.492.900.330.5300.0170.4510.0220.1950.022267.5442.511832.051826.882410.63118.962572.102.012.98
T0pH7.47.4033282696136247.012.5624858249514526421331.840.161.230.150.5300.0170.4510.0220.1950.022267.2596.773642.733631.672570.4853.162720.400.871.31
T0pH7.47.4033302568129236.012.2565877236213525051321.890.151.280.150.5300.0170.4510.0220.1950.022267.2589.623535.643525.162440.5353.652583.800.891.33
T0pH7.47.4033322827134262.012.75951028258813027491392.260.181.530.170.5300.0170.4510.0220.1950.022267.2496.173969.313957.822680.6362.602839.401.051.56
T0pH7.47.4033342526135235.012.5515985229514024441352.180.151.490.160.5300.0170.4510.0220.1950.022267.2483.763610.193599.982385.5259.122528.401.001.48
T1pH8.18.103328231418736.82.68128035163513519231606.030.954.040.610.4740.0560.3490.0480.1470.04511.2063.6166.0351.1355.0831.432267.9513.89522.97521.381848.44191.472053.803.124.71
T1pH8.18.103330264719036.92.781134030184214521901667.400.554.990.630.5500.0200.3990.0390.2160.02211.1493.2889.5123.48811.1933.063267.9515.40607.40605.592098.74230.962345.103.815.71
T1pH8.18.103332269518936.92.68136542183513822091628.020.735.440.600.4850.0320.4540.0120.1590.01813.4041.8978.9843.77210.6621.131267.9415.15625.14623.332111.54246.612373.304.126.13
T1pH8.18.103334239219032.22.67133835157913319241607.500.745.130.600.4950.0430.4020.0410.0080.0058.3034.3968.1662.7714.4770.820267.9412.94557.61556.031841.48228.082082.503.865.71
T1pH7.97.903328268516966.94.61622314214017023871703.340.103.340.090.5410.0240.3410.0440.1540.0289.3531.8286.9501.8975.9580.987267.7527.691042.491039.322325.22151.992504.902.483.74
T1pH7.97.903330262416865.14.51622403205817323141733.520.153.520.100.4910.0340.2810.0400.0960.0399.2772.4038.6954.3467.0962.006267.7526.181032.881029.822252.65156.472435.302.583.87
T1pH7.97.903332260717064.64.51522383204418022971823.490.123.490.100.4350.0650.2120.0200.1110.0326.6531.7307.1992.7563.6640.972267.7425.211040.631037.622218.72163.572407.502.734.07
T1pH7.97.903334230917056.34.61222354174317519907603.570.143.570.120.3390.0700.2790.0720.0410.0154.5380.47214.8544.82711.7054.177267.7421.61931.33928.701941.41151.782114.802.573.80
T1pH7.77.7033282532110108.04.72711481821728023471012.140.442.140.330.5330.0220.2060.1110.1430.0089.0062.2636.6160.9147.3601.080267.5543.511637.921632.952305.0395.062443.601.552.34
T1pH7.77.7033302565108110.04.7251160192185822370992.360.522.360.320.4980.0370.3120.0680.1440.0099.0062.2636.6160.9147.3601.080267.5542.771687.241682.242322.70101.842467.301.682.52
T1pH7.77.7033322427110104.04.82411621820428322281102.410.492.410.340.5310.0190.2470.0870.2590.0419.0062.2636.6160.9147.3601.080267.5439.301622.091617.402183.29101.612324.201.702.53
T1pH7.77.7033342691109115.04.8251191202244802460952.900.492.900.330.3900.0280.0760.0240.0200.0089.0062.2636.6160.9147.3601.080267.5442.511832.051826.882410.63118.962572.102.012.98
T1pH7.47.4033282696136247.012.5624858249514526421331.840.161.230.150.5220.0270.3170.0070.3250.0469.0062.2636.6160.9147.3601.080267.2596.773642.733631.672570.4853.162720.400.871.31
T1pH7.47.4033302568129236.012.2565877236213525051321.890.151.280.150.4840.0340.2220.0180.1970.0509.0062.2636.6160.9147.3601.080267.2589.623535.643525.162440.5353.652583.800.891.33
T1pH7.47.4033322827134262.012.75951028258813027491392.260.181.530.170.3770.0460.1670.0310.0880.0109.0062.2636.6160.9147.3601.080267.2496.173969.313957.822680.6362.602839.401.051.56
T1pH7.47.4033342526135235.012.5515985229514024441352.180.151.490.160.3490.0430.2250.0680.1250.0049.0062.2636.6160.9147.3601.080267.2483.763610.193599.982385.5259.122528.401.001.48
T2pH8.18.103328231418736.82.68128035163513519231606.030.954.040.610.5470.0210.3800.0220.1410.0447.1251.5055.8891.9168.2581.686267.9513.89522.97521.381848.44191.472053.803.124.71
T2pH8.18.103330264719036.92.781134030184214521901667.400.554.990.630.4770.0540.4190.0290.1830.0085.0070.6936.9841.7747.3493.160267.9515.40607.40605.592098.74230.962345.103.815.71
T2pH8.18.103332269518936.92.68136542183513822091628.020.735.440.600.4910.0320.4160.0480.1390.0169.0871.8049.3071.7077.4310.887267.9415.15625.14623.332111.54246.612373.304.126.13
T2pH8.18.103334239219032.22.67133835157913319241607.500.745.130.600.2170.0620.3190.0500.0000.0009.7802.99512.9703.8954.0130.726267.9412.94557.61556.031841.48228.082082.503.865.71
T2pH7.97.903328268516966.94.61622314214017023871703.340.103.340.090.5040.0380.2900.0290.0270.0174.7331.0508.0961.6101.7781.778267.7527.691042.491039.322325.22151.992504.902.483.74
T2pH7.97.903330262416865.14.51622403205817323141733.520.153.520.100.4790.0270.2840.0320.0800.0613.0500.7954.2420.9211.0701.070267.7526.181032.881029.822252.65156.472435.302.583.87
T2pH7.97.903332260717064.64.51522383204418022971823.490.123.490.100.3350.0820.2500.0340.0660.0384.4940.6433.9240.8081.7251.057267.7425.211040.631037.622218.72163.572407.502.734.07
T2pH7.97.903334230917056.34.61222354174317519907603.570.143.570.120.1210.0910.2530.0570.0000.0003.0782.2139.6053.2280.0000.000267.7421.61931.33928.701941.41151.782114.802.573.80
T2pH7.77.7033282532110108.04.72711481821728023471012.140.442.140.330.5010.0240.4160.0880.1670.0972.7830.5262.4590.5382.3830.508267.5543.511637.921632.952305.0395.062443.601.552.34
T2pH7.77.7033302565108110.04.7251160192185822370992.360.522.360.320.4440.0570.4340.0290.1720.1005.3191.0784.9521.6773.8110.236267.5542.771687.241682.242322.70101.842467.301.682.52
T2pH7.77.7033322427110104.04.82411621820428322281102.410.492.410.340.5180.0140.1880.0690.1860.0633.4070.4513.5730.2063.1780.340267.5439.301622.091617.402183.29101.612324.201.702.53
T2pH7.77.7033342691109115.04.8251191202244802460952.900.492.900.330.2630.0320.3180.0550.0120.0124.6440.5324.0180.7002.2830.909267.5442.511832.051826.882410.63118.962572.102.012.98
T2pH7.47.4033282696136247.012.5624858249514526421331.840.161.230.150.3480.0680.2560.0680.1080.1083.4931.1141.7250.2732.1780.480267.2596.773642.733631.672570.4853.162720.400.871.31
T2pH7.47.4033302568129236.012.2565877236213525051321.890.151.280.150.4630.0260.0590.0590.0000.0002.8890.4022.0670.2641.9080.581267.2589.623535.643525.162440.5353.652583.800.891.33
T2pH7.47.4033322827134262.012.75951028258813027491392.260.181.530.170.3950.0440.3170.0260.0440.0442.5320.3623.2441.0782.5350.707267.2496.173969.313957.822680.6362.602839.401.051.56
T2pH7.47.4033342526135235.012.5515985229514024441352.180.151.490.160.3650.0830.3610.0390.0000.0001.7580.3442.1740.4660.0000.000267.2483.763610.193599.982385.5259.122528.401.001.48
T3pH8.18.103328231418736.82.68128035163513519231606.030.954.040.610.4840.0250.3750.0510.1890.0229.0553.8396.8261.7476.5690.862267.9513.89522.97521.381848.44191.472053.803.124.71
T3pH8.18.103330264719036.92.781134030184214521901667.400.554.990.630.4880.0370.3870.0340.1120.0135.9840.8885.0130.9087.6440.349267.9515.40607.40605.592098.74230.962345.103.815.71
T3pH8.18.103332269518936.92.68136542183513822091628.020.735.440.600.4060.0330.1080.0210.1130.03814.0571.4737.8661.8148.3013.295267.9415.15625.14623.332111.54246.612373.304.126.13
T3pH8.18.103334239219032.22.67133835157913319241607.500.745.130.600.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.000267.9412.94557.61556.031841.48228.082082.503.865.71
T3pH7.97.903328268516966.94.61622314214017023871703.340.103.340.090.5260.0240.2520.0680.0000.0008.7102.81210.5372.7060.0000.000267.7527.691042.491039.322325.22151.992504.902.483.74
T3pH7.97.903330262416865.14.51622403205817323141733.520.153.520.100.4630.0540.1520.0130.0000.0005.9731.0929.6351.8180.0000.000267.7526.181032.881029.822252.65156.472435.302.583.87
T3pH7.97.903332260717064.64.51522383204418022971823.490.123.490.100.2480.0810.1720.0350.0000.0008.6322.1445.0701.6710.0000.000267.7425.211040.631037.622218.72163.572407.502.734.07
T3pH7.97.903334230917056.34.61222354174317519907603.570.143.570.120.0000.0000.2600.0450.0000.0003.2383.2388.1762.9230.0000.000267.7421.61931.33928.701941.41151.782114.802.573.80
T3pH7.77.7033282532110108.04.72711481821728023471012.140.442.140.330.3050.1070.2640.1030.0000.0002.8190.5771.7130.9890.6020.355267.5543.511637.921632.952305.0395.062443.601.552.34
T3pH7.77.7033302565108110.04.7251160192185822370992.360.522.360.320.4530.0350.1870.1100.0250.0253.1110.6852.5660.8911.3870.886267.5542.771687.241682.242322.70101.842467.301.682.52
T3pH7.77.7033322427110104.04.82411621820428322281102.410.492.410.340.4580.0220.1500.0870.0500.0001.7250.3571.7370.6121.0340.546267.5439.301622.091617.402183.29101.612324.201.702.53
T3pH7.77.7033342691109115.04.8251191202244802460952.900.492.900.330.0190.0190.1720.1020.0000.0001.4660.8640.5450.3270.0000.000267.5442.511832.051826.882410.63118.962572.102.012.98
T3pH7.47.4033282696136247.012.5624858249514526421331.840.161.230.150.3450.0460.0970.0970.1000.1000.9980.5821.5460.8930.2200.220267.2596.773642.733631.672570.4853.162720.400.871.31
T3pH7.47.4033302568129236.012.2565877236213525051321.890.151.280.150.4440.0390.0890.0890.0000.0000.9490.8060.5620.5620.1170.117267.2589.623535.643525.162440.5353.652583.800.891.33
T3pH7.47.4033322827134262.012.75951028258813027491392.260.181.530.170.1200.1200.1430.0890.0000.0001.0790.6281.4370.5850.0000.000267.2496.173969.313957.822680.6362.602839.401.051.56
T3pH7.47.4033342526135235.012.5515985229514024441352.180.151.490.160.0000.0000.1170.0700.0000.0000.8380.4930.4950.3490.0000.000267.2483.763610.193599.982385.5259.122528.401.001.48
T4pH8.18.103328231418736.82.68128035163513519231606.030.954.040.610.024200.002800.013300.00360-0.008300.00100459.69929.4146.8414.3051.7491.0615.4863.0950.6830.3609.3513.9872.3020.9690.4850.0550.3050.0410.1610.0126.7500.7675.1340.4044.7600.5200.262750.007650.333250.004010.122500.00096267.9513.89522.97521.381848.44191.472053.803.124.71
T4pH8.18.103330264719036.92.781134030184214521901667.400.554.990.630.024200.00160-0.169200.18140-0.005000.00220327.20031.9804.7322.7951.0541.4115.3314.0540.4571.1105.3314.0540.4571.1100.4640.0160.3000.0590.2040.0316.5550.8458.6732.0826.4000.3300.259500.004730.333250.006410.123000.00058267.9515.40607.40605.592098.74230.962345.103.815.71
T4pH8.18.103332269518936.92.68136542183513822091628.020.735.440.600.013300.002700.013300.00270-0.077500.014700.0000.0004.6153.2100.5860.4294.3122.3190.6140.5665.8891.8971.5250.3680.4170.0290.2710.0430.0000.0005.6050.2285.6180.4160.0000.0000.260250.006790.333000.010210.098500.00253267.9415.15625.14623.332111.54246.612373.304.126.13
T4pH8.18.103334239219032.22.67133835157913319241607.500.745.130.60-1.158300.34510-0.815000.17200-0.093300.018800.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.257000.006280.331000.009350.096000.00216267.9412.94557.61556.031841.48228.082082.503.865.71
T4pH7.97.903328268516966.94.61622314214017023871703.340.103.340.090.004200.003700.002500.00160-0.049200.010800.0000.0004.9913.3090.7030.4764.5382.7481.1260.6870.0000.0000.0000.0000.4540.0370.2920.0630.0000.0006.3460.9408.8282.0020.0000.0000.253750.002690.333000.005350.109000.00108267.7527.691042.491039.322325.22151.992504.902.483.74
T4pH7.97.903330262416865.14.51622403205817323141733.520.153.520.100.006700.003600.002500.00160-0.060000.023900.0000.0004.7473.4600.4800.3903.5432.2081.2520.7700.0000.0000.0000.0000.3970.0630.1550.0620.0000.0006.0570.4085.7952.2660.0000.0000.243000.009830.330000.005480.108250.00048267.7526.181032.881029.822252.65156.472435.302.583.87
T4pH7.97.903332260717064.64.51522383204418022971823.490.123.490.100.007500.00080-0.005000.00520-0.100000.034300.0000.0003.5432.2080.1520.1234.1442.6781.6471.0610.0000.0000.0000.0000.3170.0370.1420.0710.0000.0002.8241.1241.0560.7030.0000.0000.238500.004840.329250.006070.101250.00125267.7425.211040.631037.622218.72163.572407.502.734.07
T4pH7.97.903334230917056.34.61222354174317519907603.570.143.570.12-0.827500.26850-0.720000.06300-0.120000.028500.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.238500.005950.326000.001470.094750.00217267.7421.61931.33928.701941.41151.782114.802.573.80
T4pH7.77.7033282532110108.04.72711481821728023471012.140.442.140.330.004200.00210-0.420000.25530-0.084200.036800.0000.0003.3442.0610.4090.2741.8801.1351.1360.6870.0000.0000.0000.0000.4600.0440.1180.1180.0000.0002.2930.3101.0801.0800.0000.0000.238250.004230.324500.002100.102250.00048267.5543.511637.921632.952305.0395.062443.601.552.34
T4pH7.77.7033302565108110.04.7251160192185822370992.360.522.360.320.009200.00340-0.464200.31160-0.052500.01760178.84542.4252.7721.8430.4090.2852.3421.4841.4230.9121.1090.3951.1090.3950.4040.0640.0650.0650.0950.0951.8560.5480.2790.2790.5850.5850.238250.006020.324250.002660.096750.00165267.5542.771687.241682.242322.70101.842467.301.682.52
T4pH7.77.7033322427110104.04.82411621820428322281102.410.492.410.34-0.185000.18500-0.665000.50110-0.069200.012100.0000.0002.8971.7111.0880.6572.4021.3761.4010.8040.0000.0000.0000.0000.2930.1030.0620.0620.0000.0001.3330.7610.6930.6930.0000.0000.237000.002740.324250.007040.096500.00171267.5439.301622.091617.402183.29101.612324.201.702.53
T4pH7.77.7033342691109115.04.8251191202244802460952.900.492.900.33-0.481700.31230-0.879200.20530-0.048300.006200.0000.0000.9760.1390.6340.0812.1081.2061.2240.6990.0000.0000.0000.0000.0000.0000.0480.0480.0000.0000.0000.0000.5880.5880.0000.0000.236750.003420.324000.004020.091250.00111267.5442.511832.051826.882410.63118.962572.102.012.98
T4pH7.47.4033282696136247.012.5624858249514526421331.840.161.230.15-0.828300.46810-0.359200.21530-0.037500.00520323.27521.0432.4031.5121.4540.9550.4840.2470.4840.2471.4540.7341.4540.7340.1120.0640.1130.1130.0580.0581.2030.7670.4030.4030.3050.3050.221900.007940.319000.003140.088750.00266267.2596.773642.733631.672570.4853.162720.400.871.31
T4pH7.47.4033302568129236.012.2565877236213525051321.890.151.280.15-0.670800.42840-0.288300.16210-0.045800.004800.0000.0001.6360.9420.9880.5842.3481.1491.6100.7640.0000.0000.0000.0000.1910.1100.0520.0520.0000.0000.9900.5960.8930.8930.0000.0000.221250.006790.317250.002530.089000.00082267.2589.623535.643525.162440.5353.652583.800.891.33
T4pH7.47.4033322827134262.012.75951028258813027491392.260.181.530.17-1.270800.34460-0.407500.12930-0.041700.002900.0000.0000.0000.0000.0000.0002.2101.3221.2810.7670.0000.0000.0000.0000.0000.0000.1670.1170.0000.0000.0000.0000.7700.5420.0000.0000.221250.001930.317250.001890.087000.00168267.2496.173969.313957.822680.6362.602839.401.051.56
T4pH7.47.4033342526135235.012.5515985229514024441352.180.151.490.16-1.237500.42160-0.730800.15380-0.037500.002800.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.0000.221000.006870.316500.003070.087000.00274267.2483.763610.193599.982385.5259.122528.401.001.48