Not logged in
PANGAEA.
Data Publisher for Earth & Environmental Science

Hu, Marian Y; Tseng, Yung-Che; Stumpp, Meike; Gutowska, Magdalena A; Kiko, Rainer; Lucassen, Magnus; Melzner, Frank (2011): Seawater carbonate chemistry, mass, length and gene expression of Sepia officinalis during experiments, 2011. PANGAEA, https://doi.org/10.1594/PANGAEA.774787, Supplement to: Hu, MY et al. (2011): Elevated seawater pCO2 differentially affects branchial acid-base transporters over the course of development in the cephalopod Sepia officinalis. American Journal of Physiology, 300(5), R1100-R1114, https://doi.org/10.1152/ajpregu.00653.2010

Always quote citation above when using data! You can download the citation in several formats below.

RIS CitationBibTeX Citation

Abstract:
The specific transporters involved in maintenance of blood pH homeostasis in cephalopod molluscs have not been identified to date. Using in situ hybridization and immuno histochemical methods, we demonstrate that Na+/K+-ATPase (soNKA), a V-type H+-ATPase (soV-HA), and Na+/HCO3- cotransporter (soNBC) are co-localized in NKA-rich cells in the gills of Sepia officinalis. mRNA expression patterns of these transporters and selected metabolic genes were examined in response to moderately elevated seawater pCO2 (0.16 and 0.35 kPa) over a time-course of six weeks in different ontogenetic stages. The applied CO2 concentrations are relevant for ocean acidification scenarios projected for the coming decades. We determined strong expression changes in late stage embryos and hatchlings, with one to three log2-fold reductions in soNKA, soNBCe, socCAII and COX. In contrast, no hypercapnia induced changes in mRNA expression were observed in juveniles during both short- and long-term exposure. However a transiently increased demand of ion regulatory demand was evident during the initial acclimation reaction to elevated seawater pCO2. Gill Na+/K+-ATPase activity and protein concentration were increased by approximately 15% in during short (2-11 day), but not long term (42 day) exposure. Our findings support the hypothesis that the energy budget of adult cephalopods is not significantly compromised during long-term exposure to moderate environmental hypercapnia. However, the down regulation of ion-regulatory and metabolic genes in late stage embryos, taken together with a significant reduction in somatic growth, indicates that cephalopod early life stages are challenged by elevated seawater pCO2.
Keyword(s):
Acid-base regulation; Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Gene expression (incl. proteomics); Growth/Morphology; Laboratory experiment; Mollusca; North Atlantic; Sepia officinalis; Single species; Temperate
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
SpeciesSpeciesHu, Marian Y
CommentCommentHu, Marian Y
Experimental treatmentExp treatHu, Marian Y
Experiment dayExp daydayHu, Marian Y
SalinitySalHu, Marian Y
Salinity, standard deviationSal std dev±Hu, Marian Y
Temperature, waterTemp°CHu, Marian Y
Temperature, standard deviationT std dev±Hu, Marian Y
pHpHHu, Marian YWTW 340i pH-analyzer and WTW SenTix 81-electrodeNBS scale
10 pH, standard deviationpH std dev±Hu, Marian Y
11 Aragonite saturation stateOmega ArgHu, Marian YCalculated using CO2SYS
12 Aragonite saturation state, standard deviationOmega Arg std dev±Hu, Marian Y
13 Carbon dioxide, partial pressurepCO2PaHu, Marian YCalculated using CO2SYS
14 Carbon dioxide, partial pressure, standard deviationpCO2 std dev±Hu, Marian Y
15 Alkalinity, totalATµmol/kgHu, Marian YPotentiometric open-cell titration
16 Alkalinity, total, standard deviationAT std dev±Hu, Marian Y
17 Sepia officinalis, massS. officinalis mgHu, Marian YPrecision scale (Sartorius TE64, Sartorius AG, Germany)
18 Sepia officinalis, mass, standard deviationS. officinalis m std dev±Hu, Marian Y
19 Sepia officinalis, length, mantleS. officinalis lmmHu, Marian YStereomicroscopy (Leica)
20 Sepia officinalis, length, mantle, standard deviationS. officinalis l std dev±Hu, Marian Y
21 Sodium/Potassium adenosine triphosphatase gene expressionNa/K-ATPaseHu, Marian Ysee reference(s)
22 Sodium/Potassium adenosine triphosphatase gene expression, standard deviationNa/K-ATPase std dev±Hu, Marian Y
23 Sodium/bicarbonate cotransporter gene expressionNa/HCO3 cotranspHu, Marian Ysee reference(s)
24 Sodium/bicarbonate cotransporter gene expression, standard deviationNa/HCO3 cotransp std dev±Hu, Marian Y
25 Sodium driven chlorine/bicarbonate exchanger gene expressionNa-driven Cl/HCO3 exchangerHu, Marian Ysee reference(s)
26 Sodium driven chlorine/bicarbonate exchanger gene expression, standard deviationNa-driven Cl/HCO3 exchanger std dev±Hu, Marian Y
27 Carbonic anhydrase gene expressionCAHu, Marian Ysee reference(s)
28 Carbonic anhydrase gene expression, standard deviationCA std dev±Hu, Marian Y
29 Adenosine triphosphate synthase gene expressionATP-synthHu, Marian Ysee reference(s)
30 Adenosine triphosphate synthase gene expression, standard deviationATP-synth std dev±Hu, Marian Y
31 Cytochrome P-450 gene expressionCYP 450Hu, Marian Ysee reference(s)
32 Cytochrome P-450 gene expression, standard deviationCYP 450 std dev±Hu, Marian Y
33 Cytochrome-c-oxydase gene expressionCOXHu, Marian Ysee reference(s)
34 Cytochrome-c-oxydase gene expression, standard deviationCOX std dev±Hu, Marian Y
35 Octopine dehydrogenase gene expressionODHHu, Marian Ysee reference(s)
36 Octopine dehydrogenase gene expression, standard deviationODH std dev±Hu, Marian Y
37 Sodium/Potassium adenosine triphosphatase maximumNa/K-ATPase maxHu, Marian Ysee reference(s)
38 Sodium/Potassium adenosine triphosphatase maximum, standard deviationNa/K-ATPase max std dev±Hu, Marian Y
39 Sodium/Potassium adenosine triphosphatase relative protein concentrationNa/K-ATPase protein%Hu, Marian Ysee reference(s)
40 Sodium/Potassium adenosine triphosphatase relative protein concentration, standard deviationNa/K-ATPase protein std dev±Hu, Marian Y
41 Sodium/Potassium adenosine triphosphatase relative mRNA expressionNa/K-ATPase mRNA%Hu, Marian Ysee reference(s)
42 Sodium/Potassium adenosine triphosphatase relative mRNA expression, standard deviationNa/K-ATPase mRNA std dev±Hu, Marian Y
43 Sepia officinalis, perivitelline fluid, pHS. officinalis PVF pHHu, Marian YWTW 340i pH-analyzer and WTW SenTix 81-electrode
44 Sepia officinalis, perivitelline fluid, pCO2S. officinalis PVF pCO2kPaHu, Marian YCalculated using CO2SYS
45 Gene nameGeneHu, Marian Y
46 Sepia officinalis, mRNA gene expressionS. officinalis mRNA expressHu, Marian Ysee reference(s)
47 Sepia officinalis, mRNA gene expression, standard deviationS. officinalis mRNA express std dev±Hu, Marian Y
48 Sepia officinalis, mRNA gene expression changeS. officinalis mRNA express change%Hu, Marian Ysee reference(s)
49 Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
50 pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
51 Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
52 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
53 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
54 Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
55 Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
56 Carbon, inorganic, dissolvedDICµmol/kgHu, Marian YCalculated using CO2SYS
57 Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
58 Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2979 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


Species

Comment

Exp treat

Exp day [day]

Sal

Sal std dev [±]

Temp [°C]

T std dev [±]

pH
(NBS scale, WTW 340i pH-analyz...)
10 
pH std dev [±]
11 
Omega Arg
(Calculated using CO2SYS)
12 
Omega Arg std dev [±]
13 
pCO2 [Pa]
(Calculated using CO2SYS)
14 
pCO2 std dev [±]
15 
AT [µmol/kg]
(Potentiometric open-cell titr...)
16 
AT std dev [±]
17 
S. officinalis m [g]
(Precision scale (Sartorius TE...)
18 
S. officinalis m std dev [±]
19 
S. officinalis l [mm]
(Stereomicroscopy (Leica))
20 
S. officinalis l std dev [±]
21 
Na/K-ATPase
(see reference(s))
22 
Na/K-ATPase std dev [±]
23 
Na/HCO3 cotransp
(see reference(s))
24 
Na/HCO3 cotransp std dev [±]
25 
Na-driven Cl/HCO3 exchanger
(see reference(s))
26 
Na-driven Cl/HCO3 exchanger std dev [±]
27 
CA
(see reference(s))
28 
CA std dev [±]
29 
ATP-synth
(see reference(s))
30 
ATP-synth std dev [±]
31 
CYP 450
(see reference(s))
32 
CYP 450 std dev [±]
33 
COX
(see reference(s))
34 
COX std dev [±]
35 
ODH
(see reference(s))
36 
ODH std dev [±]
37 
Na/K-ATPase max
(see reference(s))
38 
Na/K-ATPase max std dev [±]
39 
Na/K-ATPase protein [%]
(see reference(s))
40 
Na/K-ATPase protein std dev [±]
41 
Na/K-ATPase mRNA [%]
(see reference(s))
42 
Na/K-ATPase mRNA std dev [±]
43 
S. officinalis PVF pH
(WTW 340i pH-analyzer and WTW ...)
44 
S. officinalis PVF pCO2 [kPa]
(Calculated using CO2SYS)
45 
Gene
46 
S. officinalis mRNA express
(see reference(s))
47 
S. officinalis mRNA express std dev [±]
48 
S. officinalis mRNA express change [%]
(see reference(s))
49 
CSC flag
(Calculated using seacarb afte...)
50 
pH
(Total scale, Calculated using...)
51 
CO2 [µmol/kg]
(Calculated using seacarb afte...)
52 
pCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)
53 
fCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)
54 
[HCO3]- [µmol/kg]
(Calculated using seacarb afte...)
55 
[CO3]2- [µmol/kg]
(Calculated using seacarb afte...)
56 
DIC [µmol/kg]
(Calculated using CO2SYS)
57 
Omega Arg
(Calculated using seacarb afte...)
58 
Omega Cal
(Calculated using seacarb afte...)
Sepia officinalis EmbryosControl34.940.1014.600.477.960.021.690.0670.974.132413.8149.100.16940.00886905187267.8526.80709.20706.652143.08110.122280.001.682.62
Sepia officinalis EmbryospCO2 0.14 kPa34.920.0714.620.477.630.020.840.01164.0424.482431.8956.120.17030.00497010159-0.03870.1377-1.22830.0283-0.24730.00230.52410.18740.12430.12490.23950.00030.00780.39000.08040.0013267.5161.921639.081633.212297.4054.782414.100.841.31
Sepia officinalis EmbryospCO2 0.4 kPa34.900.0514.620.457.280.030.390.02371.3528.522415.1643.000.16190.00486328167-0.77020.3894-2.13680.15330.06700.1345-1.23620.2277-0.21650.1171-0.33180.30550.90540.4672-0.14480.3472267.17140.263712.693699.392352.8825.362518.500.390.60
Sepia officinalis hatchlingControl34.940.1014.600.477.960.021.690.0670.974.132413.8149.100.22120.00777378196267.8526.80709.20706.652143.08110.122280.001.682.62
Sepia officinalis hatchlingpCO2 0.14 kPa34.920.0714.620.477.630.020.840.01164.0424.482431.8956.120.22630.00847366110-0.00010.4245-0.56710.44290.06460.1562-0.18600.38050.04120.1066-0.15640.61620.28720.2994-0.75220.3664267.5161.921639.081633.212297.4054.782414.100.841.31
Sepia officinalis hatchlingpCO2 0.4 kPa34.900.0514.620.457.280.030.390.02371.3528.522415.1643.000.19150.00596775650-0.97100.4958-2.95850.16030.03570.1576-1.53320.2074-0.26200.0886-0.83830.3169-1.25300.2661-0.08490.2957267.17140.263712.693699.392352.8825.362518.500.390.60
Sepia officinalis juvenileControl33.080.2014.810.208.210.083.530.5350.268.762827.00129.08268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenilepCO2 0.4 kPa CO233.090.2114.830.217.390.090.750.11308.9437.403127.53141.91-0.0364-0.0647-0.0234-0.0287-0.0755-0.1326-0.2038-0.0797-0.1620-0.0621-0.1245-0.0880-0.2017-0.2067-0.2826-0.0872267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenileControl233.080.2014.810.208.210.083.530.5350.268.762827.00129.0894.83363.864058.6337.86079.6297.400268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenileControl1133.080.2014.810.208.210.083.530.5350.268.762827.00129.0883.18153.526052.1376.98075.8516.310268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenileControl4233.080.2014.810.208.210.083.530.5350.268.762827.00129.0887.88094.637096.8481.22089.9286.330268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenilepCO2 0.4 kPa CO2233.090.2114.830.217.390.090.750.11308.9437.403127.53141.91112.52894.279070.7324.66074.6213.680267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenilepCO2 0.4 kPa CO21133.090.2114.830.217.390.090.750.11308.9437.403127.53141.91101.79084.438077.0343.35073.9595.650267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenilepCO2 0.4 kPa CO24233.090.2114.830.217.390.090.750.11308.9437.403127.53141.9193.25663.6990100.0001.970100.0008.920267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis embryosfinal sage of development (stage 30)Control34.940.1014.600.477.960.021.690.0670.974.132413.8149.107.3160.292267.8526.80709.20706.652143.08110.122280.001.682.62
Sepia officinalis embryosfinal sage of development (stage 30)Control34.940.1014.600.477.960.021.690.0670.974.132413.8149.107.3520.269267.8526.80709.20706.652143.08110.122280.001.682.62
Sepia officinalis embryosfinal sage of development (stage 30)Control34.940.1014.600.477.960.021.690.0670.974.132413.8149.107.4310.231267.8526.80709.20706.652143.08110.122280.001.682.62
Sepia officinalis embryosfinal sage of development (stage 30)Control34.940.1014.600.477.960.021.690.0670.974.132413.8149.107.3720.252267.8526.80709.20706.652143.08110.122280.001.682.62
Sepia officinalis embryosfinal sage of development (stage 30)Control34.940.1014.600.477.960.021.690.0670.974.132413.8149.107.4070.255267.8526.80709.20706.652143.08110.122280.001.682.62
Sepia officinalis embryosfinal sage of development (stage 30)Control34.940.1014.600.477.960.021.690.0670.974.132413.8149.107.4240.233267.8526.80709.20706.652143.08110.122280.001.682.62
Sepia officinalis embryosfinal sage of development (stage 30)Control34.940.1014.600.477.960.021.690.0670.974.132413.8149.107.4490.212267.8526.80709.20706.652143.08110.122280.001.682.62
Sepia officinalis embryosfinal sage of development (stage 30)Control34.940.1014.600.477.960.021.690.0670.974.132413.8149.107.3820.263267.8526.80709.20706.652143.08110.122280.001.682.62
Sepia officinalis embryosfinal sage of development (stage 30)Control34.940.1014.600.477.960.021.690.0670.974.132413.8149.107.4030.234267.8526.80709.20706.652143.08110.122280.001.682.62
Sepia officinalis embryosfinal sage of development (stage 30)Control34.940.1014.600.477.960.021.690.0670.974.132413.8149.107.3220.300267.8526.80709.20706.652143.08110.122280.001.682.62
Sepia officinalis embryosfinal sage of development (stage 30)Control34.940.1014.600.477.960.021.690.0670.974.132413.8149.107.3290.283267.8526.80709.20706.652143.08110.122280.001.682.62
Sepia officinalis embryosfinal sage of development (stage 30)Control34.940.1014.600.477.960.021.690.0670.974.132413.8149.107.3940.266267.8526.80709.20706.652143.08110.122280.001.682.62
Sepia officinalis embryosfinal sage of development (stage 30)Control34.940.1014.600.477.960.021.690.0670.974.132413.8149.107.4440.224267.8526.80709.20706.652143.08110.122280.001.682.62
Sepia officinalis embryosfinal sage of development (stage 30)Control34.940.1014.600.477.960.021.690.0670.974.132413.8149.107.2600.330267.8526.80709.20706.652143.08110.122280.001.682.62
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.14 kPa34.920.0714.620.477.630.020.840.01164.0424.482431.8956.127.2100.391267.5161.921639.081633.212297.4054.782414.100.841.31
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.14 kPa34.920.0714.620.477.630.020.840.01164.0424.482431.8956.127.2280.419267.5161.921639.081633.212297.4054.782414.100.841.31
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.14 kPa34.920.0714.620.477.630.020.840.01164.0424.482431.8956.127.0060.649267.5161.921639.081633.212297.4054.782414.100.841.31
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.14 kPa34.920.0714.620.477.630.020.840.01164.0424.482431.8956.127.2130.438267.5161.921639.081633.212297.4054.782414.100.841.31
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.14 kPa34.920.0714.620.477.630.020.840.01164.0424.482431.8956.127.3330.323267.5161.921639.081633.212297.4054.782414.100.841.31
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.14 kPa34.920.0714.620.477.630.020.840.01164.0424.482431.8956.127.2940.339267.5161.921639.081633.212297.4054.782414.100.841.31
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.14 kPa34.920.0714.620.477.630.020.840.01164.0424.482431.8956.127.1340.507267.5161.921639.081633.212297.4054.782414.100.841.31
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.14 kPa34.920.0714.620.477.630.020.840.01164.0424.482431.8956.127.2130.428267.5161.921639.081633.212297.4054.782414.100.841.31
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.14 kPa34.920.0714.620.477.630.020.840.01164.0424.482431.8956.127.3340.328267.5161.921639.081633.212297.4054.782414.100.841.31
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.14 kPa34.920.0714.620.477.630.020.840.01164.0424.482431.8956.127.2800.369267.5161.921639.081633.212297.4054.782414.100.841.31
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.14 kPa34.920.0714.620.477.630.020.840.01164.0424.482431.8956.127.1060.538267.5161.921639.081633.212297.4054.782414.100.841.31
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.14 kPa34.920.0714.620.477.630.020.840.01164.0424.482431.8956.127.1110.533267.5161.921639.081633.212297.4054.782414.100.841.31
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.14 kPa34.920.0714.620.477.630.020.840.01164.0424.482431.8956.127.2390.433267.5161.921639.081633.212297.4054.782414.100.841.31
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.14 kPa34.920.0714.620.477.630.020.840.01164.0424.482431.8956.127.0600.615267.5161.921639.081633.212297.4054.782414.100.841.31
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.4 kPa34.900.0514.620.457.280.030.390.02371.3528.522415.1643.007.0570.808267.17140.263712.693699.392352.8825.362518.500.390.60
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.4 kPa34.900.0514.620.457.280.030.390.02371.3528.522415.1643.007.0471.217267.17140.263712.693699.392352.8825.362518.500.390.60
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.4 kPa34.900.0514.620.457.280.030.390.02371.3528.522415.1643.007.0270.714267.17140.263712.693699.392352.8825.362518.500.390.60
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.4 kPa34.900.0514.620.457.280.030.390.02371.3528.522415.1643.007.1180.713267.17140.263712.693699.392352.8825.362518.500.390.60
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.4 kPa34.900.0514.620.457.280.030.390.02371.3528.522415.1643.006.9960.666267.17140.263712.693699.392352.8825.362518.500.390.60
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.4 kPa34.900.0514.620.457.280.030.390.02371.3528.522415.1643.006.9570.771267.17140.263712.693699.392352.8825.362518.500.390.60
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.4 kPa34.900.0514.620.457.280.030.390.02371.3528.522415.1643.007.1670.767267.17140.263712.693699.392352.8825.362518.500.390.60
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.4 kPa34.900.0514.620.457.280.030.390.02371.3528.522415.1643.006.7860.620267.17140.263712.693699.392352.8825.362518.500.390.60
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.4 kPa34.900.0514.620.457.280.030.390.02371.3528.522415.1643.007.0540.686267.17140.263712.693699.392352.8825.362518.500.390.60
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.4 kPa34.900.0514.620.457.280.030.390.02371.3528.522415.1643.007.016267.17140.263712.693699.392352.8825.362518.500.390.60
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.4 kPa34.900.0514.620.457.280.030.390.02371.3528.522415.1643.007.069267.17140.263712.693699.392352.8825.362518.500.390.60
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.4 kPa34.900.0514.620.457.280.030.390.02371.3528.522415.1643.007.007267.17140.263712.693699.392352.8825.362518.500.390.60
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.4 kPa34.900.0514.620.457.280.030.390.02371.3528.522415.1643.007.040267.17140.263712.693699.392352.8825.362518.500.390.60
Sepia officinalis embryosfinal sage of development (stage 30)pCO2 0.4 kPa34.900.0514.620.457.280.030.390.02371.3528.522415.1643.007.100267.17140.263712.693699.392352.8825.362518.500.390.60
Sepia officinalis juvenileControl233.080.2014.810.208.210.083.530.5350.268.762827.00129.08NKA7.8070.725268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenileControl233.080.2014.810.208.210.083.530.5350.268.762827.00129.08NBC2.9820.298268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenileControl233.080.2014.810.208.210.083.530.5350.268.762827.00129.08NDCBE0.2610.038268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenileControl233.080.2014.810.208.210.083.530.5350.268.762827.00129.08CA3.3270.368268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenileControl233.080.2014.810.208.210.083.530.5350.268.762827.00129.08ATP-Synthase8.0540.637268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenileControl233.080.2014.810.208.210.083.530.5350.268.762827.00129.08CYP 4500.4300.032268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenileControl233.080.2014.810.208.210.083.530.5350.268.762827.00129.08COX8.3882.409268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenileControl233.080.2014.810.208.210.083.530.5350.268.762827.00129.08ODH0.9970.074268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenile0.3 kPa233.090.2114.830.217.390.090.750.11308.9437.403127.53141.91NKA7.3160.361-6.289267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenile0.3 kPa233.090.2114.830.217.390.090.750.11308.9437.403127.53141.91NBC2.8040.253-5.970267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenile0.3 kPa233.090.2114.830.217.390.090.750.11308.9437.403127.53141.91NDCBE0.2070.010-20.770267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenile0.3 kPa233.090.2114.830.217.390.090.750.11308.9437.403127.53141.91CA3.2450.222-2.471267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenile0.3 kPa233.090.2114.830.217.390.090.750.11308.9437.403127.53141.91ATP-Synthase8.3240.2223.345267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenile0.3 kPa233.090.2114.830.217.390.090.750.11308.9437.403127.53141.91CYP 4500.4380.0341.957267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenile0.3 kPa233.090.2114.830.217.390.090.750.11308.9437.403127.53141.91COX4.1580.613-50.432267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenile0.3 kPa233.090.2114.830.217.390.090.750.11308.9437.403127.53141.91ODH1.0090.0651.205267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenileControl1033.080.2014.810.208.210.083.530.5350.268.762827.00129.08NKA7.4370.619268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenileControl1033.080.2014.810.208.210.083.530.5350.268.762827.00129.08NBC2.3670.118268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenileControl1033.080.2014.810.208.210.083.530.5350.268.762827.00129.08NDCBE0.2600.039268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenileControl1033.080.2014.810.208.210.083.530.5350.268.762827.00129.08CA3.4720.339268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenileControl1033.080.2014.810.208.210.083.530.5350.268.762827.00129.08ATP-Synthase8.4520.477268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenileControl1033.080.2014.810.208.210.083.530.5350.268.762827.00129.08CYP 4500.3820.036268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenileControl1033.080.2014.810.208.210.083.530.5350.268.762827.00129.08COX7.7242.089268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenileControl1033.080.2014.810.208.210.083.530.5350.268.762827.00129.08ODH0.9840.101268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenile0.3 kPa1033.090.2114.830.217.390.090.750.11308.9437.403127.53141.91NKA7.2510.554-2.494267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenile0.3 kPa1033.090.2114.830.217.390.090.750.11308.9437.403127.53141.91NBC2.3290.130-1.609267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenile0.3 kPa1033.090.2114.830.217.390.090.750.11308.9437.403127.53141.91NDCBE0.2470.014-5.096267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenile0.3 kPa1033.090.2114.830.217.390.090.750.11308.9437.403127.53141.91CA3.0140.325-13.174267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenile0.3 kPa1033.090.2114.830.217.390.090.750.11308.9437.403127.53141.91ATP-Synthase7.5550.548-10.618267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenile0.3 kPa1033.090.2114.830.217.390.090.750.11308.9437.403127.53141.91CYP 4500.3320.030-13.050267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenile0.3 kPa1033.090.2114.830.217.390.090.750.11308.9437.403127.53141.91COX6.3502.742-17.792267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenile0.3 kPa1033.090.2114.830.217.390.090.750.11308.9437.403127.53141.91ODH0.9020.099-8.269267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenileControl4233.080.2014.810.208.210.083.530.5350.268.762827.00129.08NKA8.8200.620268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenileControl4233.080.2014.810.208.210.083.530.5350.268.762827.00129.08NBC3.8600.190268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenileControl4233.080.2014.810.208.210.083.530.5350.268.762827.00129.08NDCBE0.2800.050268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenileControl4233.080.2014.810.208.210.083.530.5350.268.762827.00129.08CA4.4500.170268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenileControl4233.080.2014.810.208.210.083.530.5350.268.762827.00129.08ATP-Synthase7.3900.400268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenileControl4233.080.2014.810.208.210.083.530.5350.268.762827.00129.08CYP 4500.3600.040268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenileControl4233.080.2014.810.208.210.083.530.5350.268.762827.00129.08COX6.9601.600268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenileControl4233.080.2014.810.208.210.083.530.5350.268.762827.00129.08ODH1.2500.040268.0916.77441.88440.302340.56204.872562.203.164.94
Sepia officinalis juvenile0.3 kPa4233.090.2114.830.217.390.090.750.11308.9437.403127.53141.91NKA9.8040.88011.201267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenile0.3 kPa4233.090.2114.830.217.390.090.750.11308.9437.403127.53141.91NBC4.1720.3008.012267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenile0.3 kPa4233.090.2114.830.217.390.090.750.11308.9437.403127.53141.91NDCBE0.3110.01011.049267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenile0.3 kPa4233.090.2114.830.217.390.090.750.11308.9437.403127.53141.91CA4.8960.26010.120267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenile0.3 kPa4233.090.2114.830.217.390.090.750.11308.9437.403127.53141.91ATP-Synthase8.5920.65016.229267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenile0.3 kPa4233.090.2114.830.217.390.090.750.11308.9437.403127.53141.91CYP 4500.4670.05029.561267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenile0.3 kPa4233.090.2114.830.217.390.090.750.11308.9437.403127.53141.91COX12.2933.58076.681267.27143.713789.743776.213033.5140.173217.400.620.97
Sepia officinalis juvenile0.3 kPa4233.090.2114.830.217.390.090.750.11308.9437.403127.53141.91ODH1.2630.1201.044267.27143.713789.743776.213033.5140.173217.400.620.97