Franke, Andrea; Clemmesen, Catriona (2011): Seawater carbonate chemistry, mortality and hatching rate, size and mass of Clupea harengus during experiments, 2011 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.774430, Supplement to: Franke, A; Clemmesen, C (2011): Effect of ocean acidification on early life stages of Atlantic herring (Clupea harengus L.). Biogeosciences, 8(12), 3697-3707, https://doi.org/10.5194/bg-8-3697-2011
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Abstract:
Due to atmospheric accumulation of anthropogenic CO2 the partial pressure of carbon dioxide (pCO2) in surface seawater increases and the pH decreases. This process known as ocean acidification might have severe effects on marine organisms and ecosystems. The present study addresses the effect of ocean acidification on early developmental stages, the most sensitive stages in life history, of the Atlantic herring (Clupea harengus L.). Eggs of the Atlantic herring were fertilized and incubated in artificially acidified seawater (pCO2 1260, 1859, 2626, 2903, 4635 µatm) and a control treatment (pCO2 480 µatm) until the main hatch of herring larvae occurred. The development of the embryos was monitored daily and newly hatched larvae were sampled to analyze their morphometrics, and their condition by measuring the RNA/DNA ratios. Elevated pCO2 neither affected the embryogenesis nor the hatch rate. Furthermore the results showed no linear relationship betweenpCO2 and total length, dry weight, yolk sac area and otolith area of the newly hatched larvae. For pCO2 and RNA/DNA ratio, however, a significant negative linear relationship was found. The RNA concentration at hatching was reduced at higher pCO2 levels, which could lead to a decreased protein biosynthesis. The results indicate that an increased pCO2 can affect the metabolism of herring embryos negatively. Accordingly, further somatic growth of the larvae could be reduced. This can have consequences for the larval fish, since smaller and slow growing individuals have a lower survival potential due to lower feeding success and increased predation mortality. The regulatory mechanisms necessary to compensate for effects of hypercapnia could therefore lead to lower larval survival. Since the recruitment of fish seems to be determined during the early life stages, future research on the factors influencing these stages are of great importance in fisheries science.
Keyword(s):
Project(s):
Funding:
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
951 data points
Data
1 Exp treat (Stereomicroscopy (Leica)) | 2 Sal | 3 Temp [°C] (Conductivity meter (WTW, Weil...) | 4 T std dev [±] | 5 Repl [#] | 6 pH (Free scale, Conductivity mete...) | 7 pH std dev [±] | 8 AT [µmol/kg] (Titration potentiometric) | 9 AT std dev [±] | 10 DIC [µmol/kg] (Calculated using CO2SYS) | 11 DIC std dev [±] | 12 pCO2water_SST_wet [µatm] (Calculated using CO2SYS) | 13 pCO2 std dev [±] | 14 CO2 [µmol/kg] (Calculated using CO2SYS) | 15 CO2 std dev [±] | 16 [HCO3]- [µmol/kg] (Calculated using CO2SYS) | 17 [HCO3]- std dev [±] | 18 [CO3]2- [µmol/kg] (Calculated using CO2SYS) | 19 [CO3]2- std dev [±] | 20 Omega Arg (Calculated using CO2SYS) | 21 Omega Arg std dev [±] | 22 C. harengus eggs [%] (Stereomicroscopy (Leica)) | 23 Mortality [%] | 24 Hatching [%] | 25 C. harengus l [mm] (Measured) | 26 C. harengus dm [µg] (Microbalance (Sartorius)) | 27 C. harengus yolk sac a [mm2] (Stereomicroscopy (Leica)) | 28 C. harengus sagitta [µm2] (Stereomicroscopy (Leica)) | 29 C. harengus lupillus [µm2] (Stereomicroscopy (Leica)) | 30 RNA/DNA | 31 DIC [µmol/kg] (from means, Calculated using ...) | 32 CSC flag (Calculated using seacarb afte...) | 33 pH (Total scale, Calculated using...) | 34 CO2 [µmol/kg] (Calculated using seacarb afte...) | 35 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...) | 36 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...) | 37 [HCO3]- [µmol/kg] (Calculated using seacarb afte...) | 38 [CO3]2- [µmol/kg] (Calculated using seacarb afte...) | 39 Omega Arg (Calculated using seacarb afte...) | 40 Omega Cal (Calculated using seacarb afte...) |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Control | 14 | 13.6 | 0.4 | 1 | 8.08 | 0.07 | 2070.2 | 4.1 | 1989.9 | 20.5 | 480 | 81 | 21.5 | 3.4 | 1887.8 | 28.2 | 80.6 | 11.2 | 1.27 | 0.17 | 12.0 | 14.9 | 78.4 | 6.0 | 42.6 | 0.5 | 371.8 | 345.1 | 3.8 | 1990.8 | 26 | 8.03 | 21.02 | 479.33 | 477.59 | 1890.70 | 79.08 | 1.25 | 2.14 |
Control | 14 | 13.6 | 0.4 | 2 | 8.08 | 0.07 | 2070.2 | 4.1 | 1989.9 | 20.5 | 480 | 81 | 21.5 | 3.4 | 1887.8 | 28.2 | 80.6 | 11.2 | 1.27 | 0.17 | 6.5 | 7.7 | 80.2 | 6.0 | 40.6 | 0.5 | 382.7 | 314.0 | 3.2 | 1990.8 | 26 | 8.03 | 21.02 | 479.33 | 477.59 | 1890.70 | 79.08 | 1.25 | 2.14 |
Control | 14 | 13.6 | 0.4 | 3 | 8.08 | 0.07 | 2070.2 | 4.1 | 1989.9 | 20.5 | 480 | 81 | 21.5 | 3.4 | 1887.8 | 28.2 | 80.6 | 11.2 | 1.27 | 0.17 | 4.2 | 5.5 | 78.8 | 6.1 | 45.4 | 0.4 | 393.2 | 347.2 | 3.1 | 1990.8 | 26 | 8.03 | 21.02 | 479.33 | 477.59 | 1890.70 | 79.08 | 1.25 | 2.14 |
Control | 14 | 13.6 | 0.4 | 4 | 8.08 | 0.07 | 2070.2 | 4.1 | 1989.9 | 20.5 | 480 | 81 | 21.5 | 3.4 | 1887.8 | 28.2 | 80.6 | 11.2 | 1.27 | 0.17 | 4.8 | 8.2 | 79.7 | 6.2 | 38.8 | 0.4 | 388.6 | 340.7 | 1990.8 | 26 | 8.03 | 21.02 | 479.33 | 477.59 | 1890.70 | 79.08 | 1.25 | 2.14 | |
Treatment-1 | 14 | 13.6 | 0.4 | 1 | 7.67 | 0.07 | 1965.8 | 4.7 | 1981.2 | 15.4 | 1260 | 218 | 55.7 | 8.8 | 1894.1 | 11.2 | 31.5 | 4.3 | 0.49 | 0.07 | 10.7 | 12.1 | 88.1 | 6.3 | 43.5 | 0.5 | 397.0 | 337.4 | 1980.2 | 26 | 7.62 | 54.15 | 1234.60 | 1230.12 | 1895.20 | 30.85 | 0.49 | 0.84 | |
Treatment-1 | 14 | 13.6 | 0.4 | 2 | 7.67 | 0.07 | 1965.8 | 4.7 | 1981.2 | 15.4 | 1260 | 218 | 55.7 | 8.8 | 1894.1 | 11.2 | 31.5 | 4.3 | 0.49 | 0.07 | 4.0 | 6.6 | 92.2 | 6.0 | 42.6 | 0.4 | 371.3 | 340.9 | 1980.2 | 26 | 7.62 | 54.15 | 1234.60 | 1230.12 | 1895.20 | 30.85 | 0.49 | 0.84 | |
Treatment-1 | 14 | 13.6 | 0.4 | 3 | 7.67 | 0.07 | 1965.8 | 4.7 | 1981.2 | 15.4 | 1260 | 218 | 55.7 | 8.8 | 1894.1 | 11.2 | 31.5 | 4.3 | 0.49 | 0.07 | 1.9 | 1.9 | 95.7 | 6.0 | 43.1 | 0.5 | 408.5 | 363.0 | 1980.2 | 26 | 7.62 | 54.15 | 1234.60 | 1230.12 | 1895.20 | 30.85 | 0.49 | 0.84 | |
Treatment-1 | 14 | 13.6 | 0.4 | 4 | 7.67 | 0.07 | 1965.8 | 4.7 | 1981.2 | 15.4 | 1260 | 218 | 55.7 | 8.8 | 1894.1 | 11.2 | 31.5 | 4.3 | 0.49 | 0.07 | 3.6 | 6.9 | 91.1 | 7.0 | 44.9 | 0.4 | 443.9 | 391.5 | 1980.2 | 26 | 7.62 | 54.15 | 1234.60 | 1230.12 | 1895.20 | 30.85 | 0.49 | 0.84 | |
Treatment-2 | 14 | 13.6 | 0.4 | 1 | 7.49 | 0.05 | 1922.6 | 5.1 | 1977.4 | 14.9 | 1859 | 240 | 81.6 | 9.0 | 1874.9 | 8.2 | 20.9 | 1.9 | 0.33 | 0.03 | 6.4 | 8.3 | 91.7 | 6.3 | 40.2 | 0.4 | 417.0 | 379.3 | 2.8 | 1977.5 | 26 | 7.44 | 81.06 | 1848.15 | 1841.44 | 1876.24 | 20.20 | 0.32 | 0.55 |
Treatment-2 | 14 | 13.6 | 0.4 | 2 | 7.49 | 0.05 | 1922.6 | 5.1 | 1977.4 | 14.9 | 1859 | 240 | 81.6 | 9.0 | 1874.9 | 8.2 | 20.9 | 1.9 | 0.33 | 0.03 | 2.8 | 2.8 | 94.2 | 5.9 | 42.5 | 0.4 | 416.1 | 344.2 | 2.9 | 1977.5 | 26 | 7.44 | 81.06 | 1848.15 | 1841.44 | 1876.24 | 20.20 | 0.32 | 0.55 |
Treatment-2 | 14 | 13.6 | 0.4 | 3 | 7.49 | 0.05 | 1922.6 | 5.1 | 1977.4 | 14.9 | 1859 | 240 | 81.6 | 9.0 | 1874.9 | 8.2 | 20.9 | 1.9 | 0.33 | 0.03 | 3.0 | 5.5 | 87.5 | 6.2 | 53.9 | 0.6 | 428.5 | 373.0 | 3.0 | 1977.5 | 26 | 7.44 | 81.06 | 1848.15 | 1841.44 | 1876.24 | 20.20 | 0.32 | 0.55 |
Treatment-2 | 14 | 13.6 | 0.4 | 4 | 7.49 | 0.05 | 1922.6 | 5.1 | 1977.4 | 14.9 | 1859 | 240 | 81.6 | 9.0 | 1874.9 | 8.2 | 20.9 | 1.9 | 0.33 | 0.03 | 10.6 | 12.9 | 86.2 | 6.5 | 48.9 | 0.4 | 419.1 | 374.0 | 1977.5 | 26 | 7.44 | 81.06 | 1848.15 | 1841.44 | 1876.24 | 20.20 | 0.32 | 0.55 | |
Treatment-3 | 14 | 13.6 | 0.4 | 1 | 7.33 | 0.03 | 1870.2 | 4.1 | 1967.4 | 7.6 | 2626 | 197 | 115.4 | 6.6 | 1837.9 | 3.8 | 14.1 | 0.7 | 0.22 | 0.01 | 5.8 | 10.0 | 74.7 | 6.3 | 38.8 | 0.5 | 393.6 | 350.9 | 2.6 | 1967.2 | 26 | 7.28 | 114.83 | 2618.13 | 2608.63 | 1838.68 | 13.69 | 0.22 | 0.37 |
Treatment-3 | 14 | 13.6 | 0.4 | 2 | 7.33 | 0.03 | 1870.2 | 4.1 | 1967.4 | 7.6 | 2626 | 197 | 115.4 | 6.6 | 1837.9 | 3.8 | 14.1 | 0.7 | 0.22 | 0.01 | 4.5 | 6.9 | 81.3 | 6.5 | 47.5 | 0.5 | 408.4 | 379.9 | 3.0 | 1967.2 | 26 | 7.28 | 114.83 | 2618.13 | 2608.63 | 1838.68 | 13.69 | 0.22 | 0.37 |
Treatment-3 | 14 | 13.6 | 0.4 | 3 | 7.33 | 0.03 | 1870.2 | 4.1 | 1967.4 | 7.6 | 2626 | 197 | 115.4 | 6.6 | 1837.9 | 3.8 | 14.1 | 0.7 | 0.22 | 0.01 | 4.2 | 5.9 | 92.3 | 6.5 | 50.2 | 0.6 | 433.2 | 392.7 | 3.3 | 1967.2 | 26 | 7.28 | 114.83 | 2618.13 | 2608.63 | 1838.68 | 13.69 | 0.22 | 0.37 |
Treatment-3 | 14 | 13.6 | 0.4 | 4 | 7.33 | 0.03 | 1870.2 | 4.1 | 1967.4 | 7.6 | 2626 | 197 | 115.4 | 6.6 | 1837.9 | 3.8 | 14.1 | 0.7 | 0.22 | 0.01 | 2.8 | 4.0 | 93.5 | 6.4 | 49.8 | 0.5 | 390.5 | 341.8 | 1967.2 | 26 | 7.28 | 114.83 | 2618.13 | 2608.63 | 1838.68 | 13.69 | 0.22 | 0.37 | |
Treatment-4 | 14 | 13.6 | 0.4 | 1 | 7.28 | 0.03 | 1854.8 | 3.1 | 1967.1 | 8.0 | 2903 | 204 | 128.5 | 7.3 | 1826.2 | 3.0 | 12.5 | 0.6 | 0.20 | 0.01 | 3.3 | 4.8 | 81.8 | 6.3 | 52.5 | 0.5 | 427.5 | 378.4 | 3.5 | 1967.0 | 26 | 7.23 | 128.01 | 2918.63 | 2908.04 | 1826.86 | 12.13 | 0.19 | 0.33 |
Treatment-4 | 14 | 13.6 | 0.4 | 2 | 7.28 | 0.03 | 1854.8 | 3.1 | 1967.1 | 8.0 | 2903 | 204 | 128.5 | 7.3 | 1826.2 | 3.0 | 12.5 | 0.6 | 0.20 | 0.01 | 9.7 | 14.8 | 79.3 | 6.3 | 45.3 | 0.6 | 470.5 | 419.0 | 2.5 | 1967.0 | 26 | 7.23 | 128.01 | 2918.63 | 2908.04 | 1826.86 | 12.13 | 0.19 | 0.33 |
Treatment-4 | 14 | 13.6 | 0.4 | 3 | 7.28 | 0.03 | 1854.8 | 3.1 | 1967.1 | 8.0 | 2903 | 204 | 128.5 | 7.3 | 1826.2 | 3.0 | 12.5 | 0.6 | 0.20 | 0.01 | 11.0 | 18.3 | 66.3 | 6.3 | 54.3 | 0.5 | 409.3 | 371.4 | 2.7 | 1967.0 | 26 | 7.23 | 128.01 | 2918.63 | 2908.04 | 1826.86 | 12.13 | 0.19 | 0.33 |
Treatment-4 | 14 | 13.6 | 0.4 | 4 | 7.28 | 0.03 | 1854.8 | 3.1 | 1967.1 | 8.0 | 2903 | 204 | 128.5 | 7.3 | 1826.2 | 3.0 | 12.5 | 0.6 | 0.20 | 0.01 | 3.1 | 3.8 | 86.2 | 6.3 | 41.2 | 0.4 | 382.5 | 330.0 | 1967.0 | 26 | 7.23 | 128.01 | 2918.63 | 2908.04 | 1826.86 | 12.13 | 0.19 | 0.33 | |
Treatment-5 | 14 | 13.6 | 0.4 | 1 | 7.05 | 0.03 | 1737.5 | 4.9 | 1934.4 | 16.6 | 4635 | 340 | 206.0 | 18.5 | 1721.5 | 4.0 | 6.9 | 0.7 | 0.11 | 0.01 | 38.8 | 51.0 | 48.2 | 6.0 | 46.0 | 0.6 | 374.8 | 353.6 | 2.6 | 1933.6 | 26 | 7.00 | 204.95 | 4672.86 | 4655.91 | 1721.92 | 6.73 | 0.11 | 0.18 |
Treatment-5 | 14 | 13.6 | 0.4 | 2 | 7.05 | 0.03 | 1737.5 | 4.9 | 1934.4 | 16.6 | 4635 | 340 | 206.0 | 18.5 | 1721.5 | 4.0 | 6.9 | 0.7 | 0.11 | 0.01 | 5.7 | 10.1 | 85.2 | 6.3 | 45.4 | 0.4 | 406.1 | 365.9 | 2.5 | 1933.6 | 26 | 7.00 | 204.95 | 4672.86 | 4655.91 | 1721.92 | 6.73 | 0.11 | 0.18 |
Treatment-5 | 14 | 13.6 | 0.4 | 3 | 7.05 | 0.03 | 1737.5 | 4.9 | 1934.4 | 16.6 | 4635 | 340 | 206.0 | 18.5 | 1721.5 | 4.0 | 6.9 | 0.7 | 0.11 | 0.01 | 1.9 | 2.4 | 88.4 | 6.0 | 43.0 | 0.5 | 383.3 | 343.8 | 2.5 | 1933.6 | 26 | 7.00 | 204.95 | 4672.86 | 4655.91 | 1721.92 | 6.73 | 0.11 | 0.18 |
Treatment-5 | 14 | 13.6 | 0.4 | 4 | 7.05 | 0.03 | 1737.5 | 4.9 | 1934.4 | 16.6 | 4635 | 340 | 206.0 | 18.5 | 1721.5 | 4.0 | 6.9 | 0.7 | 0.11 | 0.01 | 7.2 | 7.2 | 80.2 | 6.1 | 45.0 | 0.5 | 435.1 | 391.2 | 1933.6 | 26 | 7.00 | 204.95 | 4672.86 | 4655.91 | 1721.92 | 6.73 | 0.11 | 0.18 |