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Fiorini, Sarah; Middelburg, Jack J; Gattuso, Jean-Pierre (2011): Seawater carbonate chemistry, nutrients, particulate carbon and growth rate of Emiliania huxleyi (AC472), Calcidiscus leptoporus (AC370) and Syracosphaera pulchra (AC418) during experiments, 2011 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.773860, Supplement to: Fiorini, S et al. (2011): Testing the effects of elevated pCO2 on coccolithophores (Prymnesiophyceae): comparison between haploid and diploid life stages. Journal of Phycology, 47(6), 1281–1291, https://doi.org/10.1111/j.1529-8817.2011.01080.x

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Abstract:
The response of Emiliania huxleyi (Lohmann) W. W. Hay et H. Mohler, Calcidiscus leptoporus (G. Murray et V. H. Blackman) J. Schiller, andSyracosphaera pulchra Lohmann to elevated partial pressure of carbon dioxide (pCO2) was investigated in batch cultures. We reported on the response of both haploid and diploid life stages of these three species. Growth rate, cell size, particulate inorganic carbon (PIC), and particulate organic carbon (POC) of both life stages were measured at two different pCO2 (400 and 760 parts per million [ppm]), and their organic and inorganic carbon production were calculated. The two life stages within the same species generally exhibited a similar response to elevated pCO2, the response of the haploid stage being often more pronounced than that of the diploid stage. The growth rate was consistently higher at elevated pCO2, but the response of other processes varied among species. Calcification rate of C. leptoporusand of S. pulchra did not change at elevated pCO2, whereas it increased in E. huxleyi. POC production and cell size of both life stages of S. pulchra and of the haploid stage of E. huxleyi markedly decreased at elevated pCO2. It remained unaltered in the diploid stage of E. huxleyi and C. leptoporus and increased in the haploid stage of the latter. The PIC:POC ratio increased in E. huxleyi and was constant in C. leptoporus and S. pulchra. Elevated pCO2 has a significant effect on these three coccolithophore species, the haploid stage being more sensitive. This effect must be taken into account when predicting the fate of coccolithophores in the future ocean.
Keyword(s):
Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcidiscus leptoporus; Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; South Pacific; Syracosphaera pulchra
Project(s):
European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)
European Project on Ocean Acidification (EPOCA)
Ocean Acidification International Coordination Centre (OA-ICC)
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 SpeciesSpeciesFiorini, Sarah
2 Experimental treatmentExp treatFiorini, Sarah
3 IdentificationIDFiorini, Sarah
4 Light:Dark cycleL:Dhh:hhFiorini, SarahMeasured
5 Radiation, photosynthetically activePARµmol/m2/sFiorini, Sarah
6 SalinitySalFiorini, Sarah
7 Temperature, waterTemp°CFiorini, Sarah
8 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmFiorini, SarahCalculated using seacarb
9 Carbon dioxideCO2µmol/kgFiorini, SarahCalculated using seacarb
10 Bicarbonate ion[HCO3]-µmol/kgFiorini, SarahCalculated using seacarb
11 Carbonate ion[CO3]2-µmol/kgFiorini, SarahCalculated using seacarb
12 Calcite saturation stateOmega CalFiorini, SarahCalculated using seacarb
13 Carbon, inorganic, dissolvedDICµmol/kgFiorini, SarahCalculated using seacarb
14 Alkalinity, totalATµmol/kgFiorini, SarahAlkalinity, Gran titration (Gran, 1950)
15 pHpHFiorini, SarahpH meter (Metrohm electrodes)Total scale
16 Nitrate[NO3]-µmol/lFiorini, Sarah
17 Phosphate[PO4]3-µmol/lFiorini, Sarah
18 Carbon/Nitrogen ratioC/NFiorini, Sarah
19 Carbon/Nitrogen ratio, standard deviationC/N std dev±Fiorini, Sarah
20 Emiliania huxleyiE. huxleyi#/lFiorini, SarahLemaur hemocytometer (Fisher Scientific)
21 Emiliania huxleyi, standard deviationE. huxleyi std dev±Fiorini, Sarah
22 Calcidiscus leptoporusC. leptoporus#/lFiorini, SarahLemaur hemocytometer (Fisher Scientific)
23 Calcidiscus leptoporus, standard deviationC. leptoporus std dev±Fiorini, Sarah
24 Syracosphaera pulchraS. pulchra#/lFiorini, SarahLemaur hemocytometer (Fisher Scientific)
25 Syracosphaera pulchra, standard deviationS. pulchra std dev±Fiorini, Sarah
26 Phytoplankton, cell biovolumePP biovolµm3Fiorini, Sarah
27 Phytoplankton, cell biovolume, standard deviationPhytopl biovol std dev±Fiorini, Sarah
28 Growth rateµ#/dayFiorini, SarahCalculated
29 Growth rate, standard deviationµ std dev±Fiorini, Sarah
30 Carbon, inorganic, particulate, per cellPIC/cellpg/#Fiorini, SarahCalculated
31 Particulate inorganic carbon per cell, standard deviationPIC/cell std dev±Fiorini, Sarah
32 Carbon, inorganic, particulate, production per cellPIC prod/cellpg/#/dayFiorini, SarahCalculated
33 Particulate inorganic carbon, production, standard deviationPIC prod std dev±Fiorini, Sarah
34 Particulate inorganic carbon/particulate organic carbon ratioPIC/POCFiorini, Sarah
35 Particulate inorganic carbon/particulate organic carbon ratio, standard deviationPIC/POC ratio std dev±Fiorini, Sarah
36 Carbon, organic, particulate, per cellPOC/cellpg/#Fiorini, SarahElement analyser, Thermo Finnigan flash EA 1112
37 Particulate organic carbon content per cell, standard deviationPOC cont/cell std dev±Fiorini, Sarah
38 Carbon, organic, particulate, production per cellPOC prod/cellpg/#/dayFiorini, SarahCalculated
39 Particulate organic carbon, production, standard deviationPOC prod std dev±Fiorini, Sarah
40 Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
41 Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
42 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
43 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
44 Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
45 Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
46 Carbon, inorganic, dissolvedDICµmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
47 Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
48 Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
492 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
Species		2 
Exp treat		3 
ID		4 
L:D [hh:hh]
(Measured)		5 
PAR [µmol/m2/s]		6 
Sal		7 
Temp [°C]		8 
pCO2water_SST_wet [µatm]
(Calculated using seacarb)		9 
CO2 [µmol/kg]
(Calculated using seacarb)		10 
[HCO3]- [µmol/kg]
(Calculated using seacarb)		11 
[CO3]2- [µmol/kg]
(Calculated using seacarb)		12 
Omega Cal
(Calculated using seacarb)		13 
DIC [µmol/kg]
(Calculated using seacarb)		14 
AT [µmol/kg]
(Alkalinity, Gran titration (G...)		15 
pH
(Total scale, pH meter (Metroh...)		16 
[NO3]- [µmol/l]		17 
[PO4]3- [µmol/l]		18 
C/N		19 
C/N std dev [±]		20 
E. huxleyi [#/l]
(Lemaur hemocytometer (Fisher ...)		21 
E. huxleyi std dev [±]		22 
C. leptoporus [#/l]
(Lemaur hemocytometer (Fisher ...)		23 
C. leptoporus std dev [±]		24 
S. pulchra [#/l]
(Lemaur hemocytometer (Fisher ...)		25 
S. pulchra std dev [±]		26 
PP biovol [µm3]		27 
Phytopl biovol std dev [±]		28 
µ [#/day]
(Calculated)		29 
µ std dev [±]		30 
PIC/cell [pg/#]
(Calculated)		31 
PIC/cell std dev [±]		32 
PIC prod/cell [pg/#/day]
(Calculated)		33 
PIC prod std dev [±]		34 
PIC/POC		35 
PIC/POC ratio std dev [±]		36 
POC/cell [pg/#]
(Element analyser, Thermo Finn...)		37 
POC cont/cell std dev [±]		38 
POC prod/cell [pg/#/day]
(Calculated)		39 
POC prod std dev [±]		40 
CSC flag
(Calculated using seacarb afte...)		41 
CO2 [µmol/kg]
(Calculated using seacarb afte...)		42 
pCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		43 
fCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		44 
[HCO3]- [µmol/kg]
(Calculated using seacarb afte...)		45 
[CO3]2- [µmol/kg]
(Calculated using seacarb afte...)		46 
DIC [µmol/kg]
(Calculated using seacarb afte...)		47 
Omega Arg
(Calculated using seacarb afte...)		48 
Omega Cal
(Calculated using seacarb afte...)
Emiliania huxleyi AC472Low pCO2Diploid12:12160331940313.417901814.4198422318.04161107.10.24826700030220006150.950.0190.280.30.540.00160.4150.6813.46400.99399.631823.90165.102002.472.584.00
Emiliania huxleyi AC472Low pCO2Haploid12:12160331940313.417901814.4198422318.04161106.90.23709100032020004040.920.01222.32023.0813.46400.99399.631823.90165.102002.472.584.00
Emiliania huxleyi AC472High CO2Diploid12:12160331977425.819621132.7210122247.80168126.30.53319900021990006820.990.02100.2100.30.700.01151.1151.3825.28752.96750.411970.82102.662098.761.612.49
Emiliania huxleyi AC472High CO2Haploid12:12160331977425.819621132.7210122247.80168125.70.12443000011880002240.940.00171.2161.2825.28752.96750.411970.82102.662098.761.612.49
Calcidiscus leptoporus AC370Low pCO2Diploid12:12160331939012.817681864.5196722218.05167105.90.45061000559000846270.330.0111315.2376.61.380.28829.1274.1813.04388.38387.071807.74167.451988.232.624.06
Calcidiscus leptoporus AC370Low pCO2Haploid12:12160331939012.817681864.5196722218.05167107.81.69268000642000124280.540.015013.1277.6813.04388.38387.071807.74167.451988.232.624.06
Calcidiscus leptoporus AC370High CO2Diploid12:12160331977225.519891192.9213322787.80181125.40.16162000139000759230.420.01965.8403.31.180.06810.2340.9825.91771.65769.032019.73105.212150.841.652.55
Calcidiscus leptoporus AC370High CO2Haploid12:12160331977225.519891192.9213322787.80181127.20.25965000278000262390.630.01593.7372.6825.91771.65769.032019.73105.212150.841.652.55
Syracosphaera pulchra AC418Low pCO2Diploid12:12160331941613.817891784.3198022228.03165107.70.26186000861000577230.620.023015.01910.30.210.1814415.58913.0813.78410.32408.931823.87161.341998.992.523.91
Syracosphaera pulchra AC418Low pCO2Haploid12:12160331941613.817891784.3198022228.03165107.80.2393400056000651550.530.002020.51070.7813.78410.32408.931823.87161.341998.992.523.91
Syracosphaera pulchra AC418High CO2Diploid12:12160331973024.119441192.9208822357.82165106.20.17655000252000451450.660.00222.7151.90.220.04996.3654.6824.14718.85716.421970.22107.462101.821.682.60
Syracosphaera pulchra AC418High CO2Haploid12:12160331973024.119441192.9208822357.82165106.60.11010700078800055520.610.03962.2583.9824.14718.85716.421970.22107.462101.821.682.60