Citation:

nclimate1291

Name: Seawater carbonate chemistry, length and survival of Inland silverside, Menidia beryllina, during experiments, 2012
Published: 2011
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Bottles or small containers/Aquaria (<20 L); Chordata; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Menidia beryllina; Mortality/Survival; Nekton; North Atlantic; Pelagos; Single species; Temperate

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Abstract:

Absorption of anthropogenic carbon dioxide by the world's oceans is causing mankind's 'other CO2 problem', ocean acidification. Although this process will challenge marine organisms that synthesize calcareous exoskeletons or shells, it is unclear how it will affect internally calcifying organisms, such as marine fish. Adult fish tolerate short-term exposures to CO2 levels that exceed those predicted for the next 300 years (~2,000 ppm), but potential effects of increased CO2 on growth and survival during the early life stages of fish remain poorly understood. Here we show that the exposure of early life stages of a common estuarine fish (Menidia beryllina) to CO2 concentrations expected in the world's oceans later this century caused severely reduced survival and growth rates. When compared with present-day CO2 levels (~400 ppm), exposure of M. beryllina embryos to ~1,000 ppm until one week post-hatch reduced average survival and length by 74% and 18%, respectively. The egg stage was significantly more vulnerable to high CO2-induced mortality than the post-hatch larval stage. These findings challenge the belief that ocean acidification will not affect fish populations, because even small changes in early life survival can generate large fluctuations in adult-fish abundance.

Keyword(s):

Animalia; Bottles or small containers/Aquaria (<20 L); Chordata; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Menidia beryllina; Mortality/Survival; Nekton; North Atlantic; Pelagos; Single species; Temperate

Project(s):

European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)

European Project on Ocean Acidification (EPOCA)

Ocean Acidification International Coordination Centre (OA-ICC)

Funding:

Seventh Framework Programme (FP7), grant/award no. 211384 : European Project on Ocean Acidification

Sixth Framework Programme (FP6), grant/award no. 511106 : European network of excellence for Ocean Ecosystems Analysis

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Identification	ID		Gobler, Christopher J
2	Experimental treatment	Exp treat		Gobler, Christopher J
3	Salinity	Sal		Gobler, Christopher J
4	Salinity, standard deviation	Sal std dev	±	Gobler, Christopher J
5	Temperature, water	Temp	°C	Gobler, Christopher J
6	Temperature, standard deviation	T std dev	±	Gobler, Christopher J
7	pH	pH		Gobler, Christopher J	pH meter (Orion)	NBS scale; H+ ions in µmol/l
8	pH, standard deviation	pH std dev	±	Gobler, Christopher J
9	pH	pH		Gobler, Christopher J		NBS scale; H+ ions in µmol/kg
10	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Gobler, Christopher J	Calculated using CO2SYS
11	Carbon dioxide, partial pressure, standard deviation	pCO2 std dev	±	Gobler, Christopher J
12	Calcite saturation state	Omega Cal		Gobler, Christopher J	Calculated using CO2SYS
13	Calcite saturation state, standard deviation	Omega Cal std dev	±	Gobler, Christopher J
14	Aragonite saturation state	Omega Arg		Gobler, Christopher J	Calculated using CO2SYS
15	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Gobler, Christopher J
16	Carbon, inorganic, dissolved	DIC	µmol/l	Gobler, Christopher J	Measured
17	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Gobler, Christopher J
18	Carbon, inorganic, dissolved	DIC	µmol/kg	Gobler, Christopher J	Calculated
19	Carbonate ion	[CO3]2-	µmol/l	Gobler, Christopher J	Calculated using CO2SYS
20	Carbonate ion, standard deviation	[CO3]2- std dev	±	Gobler, Christopher J
21	Alkalinity, total	AT	mmol(eq)/l	Gobler, Christopher J	Calculated using CO2SYS
22	Alkalinity, total, standard deviation	AT std dev	±	Gobler, Christopher J
23	Alkalinity, total	AT	µmol/kg	Gobler, Christopher J	Calculated using CO2SYS	From mean pH and DIC
24	Menidia beryllina, length	M. beryllina l	mm	Gobler, Christopher J	Measured	Weighed mean
25	Menidia beryllina, length, standard devitation	M. beryllina l std dev	±	Gobler, Christopher J
26	Sample ID	Sample ID		Gobler, Christopher J
27	Survival	Survival	%	Gobler, Christopher J
28	Carbonate system computation flag	CSC flag		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
29	pH	pH		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)	Total scale; H+ ions in µmol/kg
30	Carbon dioxide	CO2	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
31	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
32	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
33	Bicarbonate ion	[HCO3]-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
34	Carbonate ion	[CO3]2-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
35	Aragonite saturation state	Omega Arg		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
36	Calcite saturation state	Omega Cal		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1887 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 ID	2 Exp treat	3 Sal	4 Sal std dev [±]	5 Temp [°C]	6 T std dev [±]	7 pH (NBS scale; H+ ions in µmol/l,...)	8 pH std dev [±]	9 pH (NBS scale; H+ ions in µmol/kg)	10 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	11 pCO2 std dev [±]	12 Omega Cal (Calculated using CO2SYS)	13 Omega Cal std dev [±]	14 Omega Arg (Calculated using CO2SYS)	15 Omega Arg std dev [±]	16 DIC [µmol/l] (Measured)	17 DIC std dev [±]	18 DIC [µmol/kg] (Calculated)	19 [CO3]2- [µmol/l] (Calculated using CO2SYS)	20 [CO3]2- std dev [±]	21 AT [mmol(eq)/l] (Calculated using CO2SYS)	22 AT std dev [±]	23 AT [µmol/kg] (From mean pH and DIC, Calcula...)	24 M. beryllina l [mm] (Weighed mean, Measured)	25 M. beryllina l std dev [±]	26 Sample ID	27 Survival [%]	28 CSC flag (Calculated using seacarb afte...)	29 pH (Total scale; H+ ions in µmol/...)	30 CO2 [µmol/kg] (Calculated using seacarb afte...)	31 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	32 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	33 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	34 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	35 Omega Arg (Calculated using seacarb afte...)	36 Omega Cal (Calculated using seacarb afte...)
Experiment 1	Ambient CO2	30	1	24	1	8.21	0.010	8.05	391.2	10.97	5.37	0.180	3.49	0.110	2019	44.69	1979.7	211.8	7.1	2.304	0.026	2159.9	4.06	0.16	1	30.0	27	7.91	16.89	566.69	564.88	1825.78	137.01	2.22	3.42
Experiment 1	Ambient CO2	30	1	24	1	8.21	0.010	8.05	391.2	10.97	5.37	0.180	3.49	0.110	2019	44.69	1979.7	211.8	7.1	2.304	0.026	2159.9			2	33.0	27	7.91	16.89	566.69	564.88	1825.78	137.01	2.22	3.42
Experiment 1	Ambient CO2	30	1	24	1	8.21	0.010	8.05	391.2	10.97	5.37	0.180	3.49	0.110	2019	44.69	1979.7	211.8	7.1	2.304	0.026	2159.9			3	29.0	27	7.91	16.89	566.69	564.88	1825.78	137.01	2.22	3.42
Experiment 1	Elevated CO2, level 1	30	1	24	1	7.82	0.004	7.67	1052.4	6.81	2.36	0.020	1.53	0.020	2075	6.75	2034.6	93.0	2.4	2.176	0.010	2079.3	3.26	0.47	1	24.0	27	7.53	42.93	1440.60	1436.01	1931.35	60.31	0.98	1.51
Experiment 1	Elevated CO2, level 1	30	1	24	1	7.82	0.004	7.67	1052.4	6.81	2.36	0.020	1.53	0.020	2075	6.75	2034.6	93.0	2.4	2.176	0.010	2079.3			2	19.0	27	7.53	42.93	1440.60	1436.01	1931.35	60.31	0.98	1.51
Experiment 1	Elevated CO2, level 1	30	1	24	1	7.82	0.004	7.67	1052.4	6.81	2.36	0.020	1.53	0.020	2075	6.75	2034.6	93.0	2.4	2.176	0.010	2079.3			3	1.0	27	7.53	42.93	1440.60	1436.01	1931.35	60.31	0.98	1.51
Experiment 1	Elevated CO2, level 2	30	1	24	1	7.50	0.010	7.35	2060.9	45.96	1.18	0.170	0.77	0.170	2041	15.27	2001.2	45.5	0.4	2.044	0.014	1956.6	3.16		1	0.0	27	7.21	86.75	2910.91	2901.63	1886.03	28.46	0.46	0.71
Experiment 1	Elevated CO2, level 2	30	1	24	1	7.50	0.010	7.35	2060.9	45.96	1.18	0.170	0.77	0.170	2041	15.27	2001.2	45.5	0.4	2.044	0.014	1956.6			2	1.0	27	7.21	86.75	2910.91	2901.63	1886.03	28.46	0.46	0.71
Experiment 1	Elevated CO2, level 2	30	1	24	1	7.50	0.010	7.35	2060.9	45.96	1.18	0.170	0.77	0.170	2041	15.27	2001.2	45.5	0.4	2.044	0.014	1956.6			3	0.0	27	7.21	86.75	2910.91	2901.63	1886.03	28.46	0.46	0.71
Experiment 2	Ambient CO2	30	1	24	1	8.16	0.008	8.00	396.0	14.40	4.22	0.142	2.74	0.095	1781	46.70	1746.3	169.0	5.6	2.015	0.051	1893.0	3.93	0.03	1	45.0	27	7.86	16.75	562.18	560.39	1620.72	108.83	1.76	2.72
Experiment 2	Ambient CO2	30	1	24	1	8.16	0.008	8.00	396.0	14.40	4.22	0.142	2.74	0.095	1781	46.70	1746.3	169.0	5.6	2.015	0.051	1893.0			2	52.0	27	7.86	16.75	562.18	560.39	1620.72	108.83	1.76	2.72
Experiment 2	Ambient CO2	30	1	24	1	8.16	0.008	8.00	396.0	14.40	4.22	0.142	2.74	0.095	1781	46.70	1746.3	169.0	5.6	2.015	0.051	1893.0			3	46.0	27	7.86	16.75	562.18	560.39	1620.72	108.83	1.76	2.72
Experiment 2	Ambient CO2	30	1	24	1	8.16	0.008	8.00	396.0	14.40	4.22	0.142	2.74	0.095	1781	46.70	1746.3	169.0	5.6	2.015	0.051	1893.0			4	64.0	27	7.86	16.75	562.18	560.39	1620.72	108.83	1.76	2.72
Experiment 2	Elevated CO2, level 1	30	1	24	1	8.00	0.010	7.85	650.0	5.40	3.43	0.141	2.23	0.092	2004	42.60	1965.0	137.0	5.7	2.180	0.049	2064.8	3.73	0.15	1	35.0	27	7.71	27.26	914.79	911.88	1850.51	87.19	1.41	2.18
Experiment 2	Elevated CO2, level 1	30	1	24	1	8.00	0.010	7.85	650.0	5.40	3.43	0.141	2.23	0.092	2004	42.60	1965.0	137.0	5.7	2.180	0.049	2064.8			2	47.0	27	7.71	27.26	914.79	911.88	1850.51	87.19	1.41	2.18
Experiment 2	Elevated CO2, level 1	30	1	24	1	8.00	0.010	7.85	650.0	5.40	3.43	0.141	2.23	0.092	2004	42.60	1965.0	137.0	5.7	2.180	0.049	2064.8			3	29.0	27	7.71	27.26	914.79	911.88	1850.51	87.19	1.41	2.18
Experiment 2	Elevated CO2, level 1	30	1	24	1	8.00	0.010	7.85	650.0	5.40	3.43	0.141	2.23	0.092	2004	42.60	1965.0	137.0	5.7	2.180	0.049	2064.8			4	45.0	27	7.71	27.26	914.79	911.88	1850.51	87.19	1.41	2.18
Experiment 2	Elevated CO2, level 2	30	1	24	1	7.88	0.013	7.72	814.0	30.80	2.39	0.119	1.55	0.078	1845	60.40	1809.1	96.0	4.8	1.961	0.064	1866.5	3.25	0.09	1	12.0	27	7.58	33.73	1131.67	1128.07	1714.82	60.52	0.98	1.51
Experiment 2	Elevated CO2, level 2	30	1	24	1	7.88	0.013	7.72	814.0	30.80	2.39	0.119	1.55	0.078	1845	60.40	1809.1	96.0	4.8	1.961	0.064	1866.5			2	12.0	27	7.58	33.73	1131.67	1128.07	1714.82	60.52	0.98	1.51
Experiment 2	Elevated CO2, level 2	30	1	24	1	7.88	0.013	7.72	814.0	30.80	2.39	0.119	1.55	0.078	1845	60.40	1809.1	96.0	4.8	1.961	0.064	1866.5			3	18.0	27	7.58	33.73	1131.67	1128.07	1714.82	60.52	0.98	1.51
Experiment 2	Elevated CO2, level 2	30	1	24	1	7.88	0.013	7.72	814.0	30.80	2.39	0.119	1.55	0.078	1845	60.40	1809.1	96.0	4.8	1.961	0.064	1866.5			4	16.0	27	7.58	33.73	1131.67	1128.07	1714.82	60.52	0.98	1.51
Experiment 2	Elevated CO2, level 3	30	1	24	1	7.73	0.008	7.58	1068.0	22.90	1.61	0.071	1.05	0.047	1718	50.03	1684.5	64.0	2.8	1.784	0.053	1704.3	3.22	0.14	1	5.0	27	7.44	43.59	1462.64	1457.97	1600.17	40.78	0.66	1.02
Experiment 2	Elevated CO2, level 3	30	1	24	1	7.73	0.008	7.58	1068.0	22.90	1.61	0.071	1.05	0.047	1718	50.03	1684.5	64.0	2.8	1.784	0.053	1704.3			2	8.0	27	7.44	43.59	1462.64	1457.97	1600.17	40.78	0.66	1.02
Experiment 2	Elevated CO2, level 3	30	1	24	1	7.73	0.008	7.58	1068.0	22.90	1.61	0.071	1.05	0.047	1718	50.03	1684.5	64.0	2.8	1.784	0.053	1704.3			3	6.0	27	7.44	43.59	1462.64	1457.97	1600.17	40.78	0.66	1.02
Experiment 2	Elevated CO2, level 3	30	1	24	1	7.73	0.008	7.58	1068.0	22.90	1.61	0.071	1.05	0.047	1718	50.03	1684.5	64.0	2.8	1.784	0.053	1704.3			4	7.0	27	7.44	43.59	1462.64	1457.97	1600.17	40.78	0.66	1.02
Experiment 3	Ambient CO 2	30	1	24	1	8.09	0.023	7.94	410.0	10.97	3.24	0.340	2.10	0.210	1567	90.94	1536.5	129.5	14.0	1.751	0.109	1650.0	4.16	0.07	1	61.0	27	7.80	17.27	579.37	577.52	1436.28	82.93	1.34	2.07
Experiment 3	Ambient CO 2	30	1	24	1	8.09	0.023	7.94	410.0	10.97	3.24	0.340	2.10	0.210	1567	90.94	1536.5	129.5	14.0	1.751	0.109	1650.0			2	87.0	27	7.80	17.27	579.37	577.52	1436.28	82.93	1.34	2.07
Experiment 3	Ambient CO 2	30	1	24	1	8.09	0.023	7.94	410.0	10.97	3.24	0.340	2.10	0.210	1567	90.94	1536.5	129.5	14.0	1.751	0.109	1650.0			3	67.0	27	7.80	17.27	579.37	577.52	1436.28	82.93	1.34	2.07
Experiment 3	Ambient CO 2	30	1	24	1	8.09	0.023	7.94	410.0	10.97	3.24	0.340	2.10	0.210	1567	90.94	1536.5	129.5	14.0	1.751	0.109	1650.0			4	39.0	27	7.80	17.27	579.37	577.52	1436.28	82.93	1.34	2.07
Experiment 3	Elevated CO2	30	1	24	1	7.80	0.017	7.64	781.2	12.23	1.62	0.150	1.05	0.090	1481	77.26	1452.2	64.9	6.0	1.560	0.084	1486.2	3.38	0.07	1	3.0	27	7.50	32.49	1090.04	1086.57	1379.04	40.64	0.66	1.01
Experiment 3	Elevated CO2	30	1	24	1	7.80	0.017	7.64	781.2	12.23	1.62	0.150	1.05	0.090	1481	77.26	1452.2	64.9	6.0	1.560	0.084	1486.2			2	29.0	27	7.50	32.49	1090.04	1086.57	1379.04	40.64	0.66	1.01
Experiment 3	Elevated CO2	30	1	24	1	7.80	0.017	7.64	781.2	12.23	1.62	0.150	1.05	0.090	1481	77.26	1452.2	64.9	6.0	1.560	0.084	1486.2			3	17.0	27	7.50	32.49	1090.04	1086.57	1379.04	40.64	0.66	1.01
Experiment 3	Elevated CO2	30	1	24	1	7.80	0.017	7.64	781.2	12.23	1.62	0.150	1.05	0.090	1481	77.26	1452.2	64.9	6.0	1.560	0.084	1486.2			4	13.0	27	7.50	32.49	1090.04	1086.57	1379.04	40.64	0.66	1.01
Experiment 4	Ambient CO 2	30	1	24	1	8.09	0.020	7.93	394.8	8.00	3.12	0.150	2.02	0.090	1509	26.32	1479.6	124.7	6.0	1.689	0.035	1588.5			1	44.4	27	7.79	16.91	567.45	565.64	1384.07	78.63	1.27	1.96
Experiment 4	Ambient CO 2	30	1	24	1	8.09	0.020	7.93	394.8	8.00	3.12	0.150	2.02	0.090	1509	26.32	1479.6	124.7	6.0	1.689	0.035	1588.5			2	33.3	27	7.79	16.91	567.45	565.64	1384.07	78.63	1.27	1.96
Experiment 4	Ambient CO 2	30	1	24	1	8.09	0.020	7.93	394.8	8.00	3.12	0.150	2.02	0.090	1509	26.32	1479.6	124.7	6.0	1.689	0.035	1588.5			3	35.6	27	7.79	16.91	567.45	565.64	1384.07	78.63	1.27	1.96
Experiment 4	Ambient CO 2	30	1	24	1	8.09	0.020	7.93	394.8	8.00	3.12	0.150	2.02	0.090	1509	26.32	1479.6	124.7	6.0	1.689	0.035	1588.5			4	37.8	27	7.79	16.91	567.45	565.64	1384.07	78.63	1.27	1.96
Experiment 4	Ambient CO 2	30	1	24	1	8.09	0.020	7.93	394.8	8.00	3.12	0.150	2.02	0.090	1509	26.32	1479.6	124.7	6.0	1.689	0.035	1588.5			5	44.4	27	7.79	16.91	567.45	565.64	1384.07	78.63	1.27	1.96
Experiment 4	Ambient CO 2	30	1	24	1	8.09	0.020	7.93	394.8	8.00	3.12	0.150	2.02	0.090	1509	26.32	1479.6	124.7	6.0	1.689	0.035	1588.5			6	35.6	27	7.79	16.91	567.45	565.64	1384.07	78.63	1.27	1.96
Experiment 4	Elevated CO2	30	1	24	1	7.75	0.070	7.60	891.5	28.14	1.47	0.410	0.95	0.270	1502	199.10	1472.7	58.8	17.0	1.568	0.223	1497.0			1	17.8	27	7.46	36.50	1224.59	1220.69	1399.02	37.23	0.60	0.93
Experiment 4	Elevated CO2	30	1	24	1	7.75	0.070	7.60	891.5	28.14	1.47	0.410	0.95	0.270	1502	199.10	1472.7	58.8	17.0	1.568	0.223	1497.0			2	6.7	27	7.46	36.50	1224.59	1220.69	1399.02	37.23	0.60	0.93
Experiment 4	Elevated CO2	30	1	24	1	7.75	0.070	7.60	891.5	28.14	1.47	0.410	0.95	0.270	1502	199.10	1472.7	58.8	17.0	1.568	0.223	1497.0			3	2.2	27	7.46	36.50	1224.59	1220.69	1399.02	37.23	0.60	0.93
Experiment 4	Elevated CO2	30	1	24	1	7.75	0.070	7.60	891.5	28.14	1.47	0.410	0.95	0.270	1502	199.10	1472.7	58.8	17.0	1.568	0.223	1497.0			4	40.0	27	7.46	36.50	1224.59	1220.69	1399.02	37.23	0.60	0.93
Experiment 4	Elevated CO2	30	1	24	1	7.75	0.070	7.60	891.5	28.14	1.47	0.410	0.95	0.270	1502	199.10	1472.7	58.8	17.0	1.568	0.223	1497.0			5	15.6	27	7.46	36.50	1224.59	1220.69	1399.02	37.23	0.60	0.93
Experiment 4	Elevated CO2	30	1	24	1	7.75	0.070	7.60	891.5	28.14	1.47	0.410	0.95	0.270	1502	199.10	1472.7	58.8	17.0	1.568	0.223	1497.0			6	8.9	27	7.46	36.50	1224.59	1220.69	1399.02	37.23	0.60	0.93
Experiment 5	Ambient CO 2	30	1	24	1	8.16	0.039	8.00	412.8	23.30	4.56	0.430	2.96	0.280	1893	94.20	1856.1	182.1	17.6	2.140	0.129	2009.9			1	46.0	27	7.86	17.67	592.94	591.05	1721.98	116.48	1.89	2.91
Experiment 5	Ambient CO 2	30	1	24	1	8.16	0.039	8.00	412.8	23.30	4.56	0.430	2.96	0.280	1893	94.20	1856.1	182.1	17.6	2.140	0.129	2009.9			2	50.0	27	7.86	17.67	592.94	591.05	1721.98	116.48	1.89	2.91
Experiment 5	Ambient CO 2	30	1	24	1	8.16	0.039	8.00	412.8	23.30	4.56	0.430	2.96	0.280	1893	94.20	1856.1	182.1	17.6	2.140	0.129	2009.9			3	70.0	27	7.86	17.67	592.94	591.05	1721.98	116.48	1.89	2.91
Experiment 5	Ambient CO 2	30	1	24	1	8.16	0.039	8.00	412.8	23.30	4.56	0.430	2.96	0.280	1893	94.20	1856.1	182.1	17.6	2.140	0.129	2009.9			4	42.0	27	7.86	17.67	592.94	591.05	1721.98	116.48	1.89	2.91
Experiment 5	Ambient CO 2	30	1	24	1	8.16	0.039	8.00	412.8	23.30	4.56	0.430	2.96	0.280	1893	94.20	1856.1	182.1	17.6	2.140	0.129	2009.9			5	54.0	27	7.86	17.67	592.94	591.05	1721.98	116.48	1.89	2.91
Experiment 5	Elevated CO2	30	1	24	1	7.84	0.027	7.69	962.0	53.90	2.41	0.230	1.57	0.150	2010	95.90	1970.9	96.5	9.3	2.119	0.105	2020.5			1	10.0	27	7.55	39.80	1335.58	1331.33	1870.06	60.99	0.99	1.52
Experiment 5	Elevated CO2	30	1	24	1	7.84	0.027	7.69	962.0	53.90	2.41	0.230	1.57	0.150	2010	95.90	1970.9	96.5	9.3	2.119	0.105	2020.5			2	24.0	27	7.55	39.80	1335.58	1331.33	1870.06	60.99	0.99	1.52
Experiment 5	Elevated CO2	30	1	24	1	7.84	0.027	7.69	962.0	53.90	2.41	0.230	1.57	0.150	2010	95.90	1970.9	96.5	9.3	2.119	0.105	2020.5			3	12.0	27	7.55	39.80	1335.58	1331.33	1870.06	60.99	0.99	1.52
Experiment 5	Elevated CO2	30	1	24	1	7.84	0.027	7.69	962.0	53.90	2.41	0.230	1.57	0.150	2010	95.90	1970.9	96.5	9.3	2.119	0.105	2020.5			4	36.0	27	7.55	39.80	1335.58	1331.33	1870.06	60.99	0.99	1.52
Experiment 5	Elevated CO2	30	1	24	1	7.84	0.027	7.69	962.0	53.90	2.41	0.230	1.57	0.150	2010	95.90	1970.9	96.5	9.3	2.119	0.105	2020.5			5	32.0	27	7.55	39.80	1335.58	1331.33	1870.06	60.99	0.99	1.52