Not logged in
PANGAEA.
Data Publisher for Earth & Environmental Science

Melatunan, Sedercor; Calosi, Piero; Rundle, Simon; Moody, A John; Widdicombe, Stephen (2011): Seawater carbonate chemistry and energy status in the periwinkle Littorina littorea during experiments, 2011 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.771574, Supplement to: Melatunan, S et al. (2011): Exposure to elevated temperature and pCO2 reduces respiration rate and energy status in the periwinkle Littorina littorea. Physiological and Biochemical Zoology, 84(6), 583-594, https://doi.org/10.1086/662680

Always quote citation above when using data! You can download the citation in several formats below.

RIS CitationBibTeX Citation

Abstract:
In the future, marine organisms will face the challenge of coping with multiple environmental changes associated with increased levels of atmospheric Pco2, such as ocean warming and acidification. To predict how organisms may or may not meet these challenges, an in-depth understanding of the physiological and biochemical mechanisms underpinning organismal responses to climate change is needed. Here, we investigate the effects of elevated Pco2 and temperature on the whole-organism and cellular physiology of the periwinkle Littorina littorea. Metabolic rates (measured as respiration rates), adenylate energy nucleotide concentrations and indexes, and end-product metabolite concentrations were measured. Compared with values for control conditions, snails decreased their respiration rate by 31% in response to elevated Pco2 and by 15% in response to a combination of increased Pco2 and temperature. Decreased respiration rates were associated with metabolic reduction and an increase in end-product metabolites in acidified treatments, indicating an increased reliance on anaerobic metabolism. There was also an interactive effect of elevated Pco2 and temperature on total adenylate nucleotides, which was apparently compensated for by the maintenance of adenylate energy charge via AMP deaminase activity. Our findings suggest that marine intertidal organisms are likely to exhibit complex physiological responses to future environmental drivers, with likely negative effects on growth, population dynamics, and, ultimately, ecosystem processes.
Keyword(s):
Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Litiorina littorea; Mollusca; North Atlantic; Respiration; Single species; Temperate; Temperature
Project(s):
European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)
European Project on Ocean Acidification (EPOCA)
Ocean Acidification International Coordination Centre (OA-ICC)
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 Experimental treatmentExp treatMelatunan, Sedercor
2 SpeciesSpeciesMelatunan, Sedercor
3 OxygenO2µmol/lMelatunan, Sedercor
4 Oxygen, standard deviationO2 std dev±Melatunan, Sedercor
5 SalinitySalMelatunan, SedercorYSI Multiparameter system
6 Salinity, standard deviationSal std dev±Melatunan, Sedercor
7 Temperature, waterTemp°CMelatunan, SedercorYSI Multiparameter system
8 Temperature, standard deviationT std dev±Melatunan, Sedercor
9 pHpHMelatunan, SedercorpH meter (Mettler Toledo, USA)NBS scale
10 pH, standard deviationpH std dev±Melatunan, Sedercor
11 Carbon, inorganic, dissolvedDICµmol/kgMelatunan, SedercorAutomated CO2 analyzer (CIBA-Corning 965, UK)
12 Carbon, inorganic, dissolved, standard deviationDIC std dev±Melatunan, Sedercor
13 Alkalinity, totalATµmol/kgMelatunan, SedercorCalculated using CO2SYS
14 Alkalinity, total, standard deviationAT std dev±Melatunan, Sedercor
15 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmMelatunan, SedercorCalculated using CO2SYS
16 Carbon dioxide, partial pressure, standard deviationpCO2 std dev±Melatunan, Sedercor
17 Bicarbonate ion[HCO3]-µmol/kgMelatunan, SedercorCalculated using CO2SYS
18 Aragonite saturation state, standard deviationOmega Arg std dev±Melatunan, Sedercor
19 Carbonate ion[CO3]2-µmol/kgMelatunan, SedercorCalculated using CO2SYS
20 Carbonate ion, standard deviation[CO3]2- std dev±Melatunan, Sedercor
21 Calcite saturation stateOmega CalMelatunan, SedercorCalculated using CO2SYS
22 Calcite saturation state, standard deviationOmega Cal std dev±Melatunan, Sedercor
23 Aragonite saturation stateOmega ArgMelatunan, SedercorCalculated using CO2SYS
24 Aragonite saturation state, standard deviationOmega Arg std dev±Melatunan, Sedercor
25 Respiration rate, oxygenResp O2µmol/mg/hMelatunan, SedercorCalculated, see reference(s)
26 Oxygen consumption, standard deviationO2 con std dev±Melatunan, Sedercor
27 Littorina littorea, adenosine triphosphate concentrationL. littorea ATPµmol/gMelatunan, Sedercorsee reference(s)
28 Littorina littorea, adenosine triphosphate concentration, standard deviationL. littorea ATP std dev±Melatunan, Sedercor
29 Littorina littorea, adenosine diphosphate concentrationL. littorea ADPµmol/gMelatunan, Sedercorsee reference(s)
30 Littorina littorea, adenosine diphosphate concentration, standard deviationL. littorea ADP std dev±Melatunan, Sedercor
31 Littorina littorea, adenosine monophosphate concentrationL. littorea AMPµmol/gMelatunan, Sedercorsee reference(s)
32 Littorina littorea, adenosine monophosphate concentration, standard deviationL. littorea AMP std dev±Melatunan, Sedercor
33 Adenylate energy charge ratioAEC ratioMelatunan, Sedercorsee reference(s)
34 Adenylate energy charge ratio, standard deviationAEC ratio std dev±Melatunan, Sedercor
35 Littorina littorea, total adenylate nucleotidesL. littorea TANµmol/gMelatunan, Sedercorsee reference(s)
36 Littorina littorea, total adenylate nucleotides, standard deviationL. littorea TAN std dev±Melatunan, Sedercor
37 Littorina littorea, succinateL. littorea succinateµmol/gMelatunan, Sedercorsee reference(s)
38 Littorina littorea, succinate, standard deviationL. littorea succinate std dev±Melatunan, Sedercor
39 Littorina littorea, D-lactateL. littorea D-lactateµmol/gMelatunan, Sedercorsee reference(s)
40 Littorina littorea, D-lactate, standard deviationL. littorea D-lactate std dev±Melatunan, Sedercor
41 Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
42 pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
43 Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
44 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
45 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
46 Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
47 Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
48 Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
49 Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
196 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
Exp treat		2 
Species		3 
O2 [µmol/l]		4 
O2 std dev [±]		5 
Sal
(YSI Multiparameter system)		6 
Sal std dev [±]		7 
Temp [°C]
(YSI Multiparameter system)		8 
T std dev [±]		9 
pH
(NBS scale, pH meter (Mettler ...)		10 
pH std dev [±]		11 
DIC [µmol/kg]
(Automated CO2 analyzer (CIBA-...)		12 
DIC std dev [±]		13 
AT [µmol/kg]
(Calculated using CO2SYS)		14 
AT std dev [±]		15 
pCO2water_SST_wet [µatm]
(Calculated using CO2SYS)		16 
pCO2 std dev [±]		17 
[HCO3]- [µmol/kg]
(Calculated using CO2SYS)		18 
Omega Arg std dev [±]		19 
[CO3]2- [µmol/kg]
(Calculated using CO2SYS)		20 
[CO3]2- std dev [±]		21 
Omega Cal
(Calculated using CO2SYS)		22 
Omega Cal std dev [±]		23 
Omega Arg
(Calculated using CO2SYS)		24 
Omega Arg std dev [±]		25 
Resp O2 [µmol/mg/h]
(Calculated, see reference(s))		26 
O2 con std dev [±]		27 
L. littorea ATP [µmol/g]
(see reference(s))		28 
L. littorea ATP std dev [±]		29 
L. littorea ADP [µmol/g]
(see reference(s))		30 
L. littorea ADP std dev [±]		31 
L. littorea AMP [µmol/g]
(see reference(s))		32 
L. littorea AMP std dev [±]		33 
AEC ratio
(see reference(s))		34 
AEC ratio std dev [±]		35 
L. littorea TAN [µmol/g]
(see reference(s))		36 
L. littorea TAN std dev [±]		37 
L. littorea succinate [µmol/g]
(see reference(s))		38 
L. littorea succinate std dev [±]		39 
L. littorea D-lactate [µmol/g]
(see reference(s))		40 
L. littorea D-lactate std dev [±]		41 
CSC flag
(Calculated using seacarb afte...)		42 
pH
(Total scale, Calculated using...)		43 
CO2 [µmol/kg]
(Calculated using seacarb afte...)		44 
pCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		45 
fCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		46 
[HCO3]- [µmol/kg]
(Calculated using seacarb afte...)		47 
[CO3]2- [µmol/kg]
(Calculated using seacarb afte...)		48 
Omega Arg
(Calculated using seacarb afte...)		49 
Omega Cal
(Calculated using seacarb afte...)
ControlLitiorina littorea0.2160.000334.770.0314.430.068.030.01159650172652428171489479042.140.081.370.0538302102.150.100.660.080.370.040.790.013.180.161.400.070.900.08267.9216.03421.50419.991491.2688.721.362.12
Hi TLitiorina littorea0.2270.004835.120.0520.910.098.040.011573401743444281314443611452.730.121.780.0832302001.010.080.470.050.190.030.750.031.670.121.620.111.930.12267.9213.17418.36416.971446.79113.041.762.70
Hi pCO2Litiorina littorea0.2140.005435.060.0414.890.057.670.01159640162740998301518384110.970.030.620.0226401801.010.070.430.060.240.060.730.061.680.112.320.142.540.28267.5536.96987.26983.731518.6940.350.620.96
Hi pCO2 and Hi TLitiorina littorea0.2190.005435.120.0720.680.087.650.001723461767481185331633445221.230.040.800.0331801800.790.040.250.040.120.030.800.031.160.071.260.091.510.11267.5337.361179.391175.481634.9450.690.791.21