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Brading, Patrick; Warner, Mark E; Davey, Philip; Smith, David J; Achterberg, Eric Pieter; Suggett, David J (2011): Seawater carbonate chemistry and growth rate during experiments with phylotypes of Symbiodinium (Dinophyceae), 2011. PANGAEA, https://doi.org/10.1594/PANGAEA.771293, Supplement to: Brading, P et al. (2011): Differential effects of ocean acidification on growth and photosynthesis among phylotypes of Symbiodinium (Dinophyceae). Limnology and Oceanography, 56(3), 927-938, https://doi.org/10.4319/lo.2011.56.3.0927

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Abstract:
We investigated the effect of elevated partial pressure of CO2 (pCO2) on the photosynthesis and growth of four phylotypes (ITS2 types A1, A13, A2, and B1) from the genus Symbiodinium, a diverse dinoflagellate group that is important, both free-living and in symbiosis, for the viability of cnidarians and is thus a potentially important model dinoflagellate group. The response of Symbiodinium to an elevated pCO2 was phylotype-specific. Phylotypes A1 and B1 were largely unaffected by a doubling in pCO2 in contrast, the growth rate of A13 and the photosynthetic capacity of A2 both increased by ~ 60%. In no case was there an effect of ocean acidification (OA) upon respiration (dark- or light-dependent) for any of the phylotypes examined. Our observations suggest that OA might preferentially select among free-living populations of Symbiodinium, with implications for future symbioses that rely on algal acquisition from the environment (i.e., horizontal transmission). Furthermore, the carbon environment within the host could differentially affect the physiology of different Symbiodinium phylotypes. The range of responses we observed also highlights that the choice of species is an important consideration in OA research and that further investigation across phylogenetic diversity, for both the direction of effect and the underlying mechanism(s) involved, is warranted.
Keyword(s):
Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Myzozoa; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Symbiodinium sp.
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
IdentificationIDBrading, Patrick
Experimental treatmentExp treatBrading, Patrick
SalinitySalBrading, Patrick
Temperature, waterTemp°CBrading, Patrick
Radiation, photosynthetically activePARµmol/m2/sBrading, Patrick
pHpHBrading, PatrickRoss Ultra pH electrode (model 8103BNUWP, Thermo Fisher Scientific)Total scale
Alkalinity, totalATµmol/kgBrading, PatrickCalculated using CO2SYS
Alkalinity, total, standard errorAT std e±Brading, Patrick
Carbon, inorganic, dissolvedDICµmol/kgBrading, PatrickTOC analyzer (Shimadzu)
10 Carbon, inorganic, dissolved, standard errorDIC std e±Brading, Patrick
11 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmBrading, PatrickCalculated using CO2SYS
12 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard errorpCO2water_SST_wet std e±Brading, Patrick
13 Bicarbonate ion[HCO3]-µmol/kgBrading, PatrickCalculated using CO2SYS
14 Bicarbonate ion, standard error[HCO3]- std e±Brading, Patrick
15 Carbonate ion[CO3]2-µmol/kgBrading, PatrickCalculated using CO2SYS
16 Carbonate ion, standard error[CO3]2- std e±Brading, Patrick
17 Carbon dioxideCO2µmol/kgBrading, PatrickCalculated using CO2SYS
18 Carbon dioxide, standard errorCO2 std e±Brading, Patrick
19 Calcite saturation stateOmega CalBrading, PatrickCalculated using CO2SYS
20 Calcite saturation state, standard deviationOmega Cal std dev±Brading, Patrick
21 Aragonite saturation stateOmega ArgBrading, PatrickCalculated using CO2SYS
22 Aragonite saturation state, standard deviationOmega Arg std dev±Brading, Patrick
23 Maximum potential capacity of photosynthesis, oxygen production, per cellPmax O2/cellfmol/#/hBrading, Patrick
24 Photosynthetic efficiency per cell, oxygen productionalpha/cell(fmol/#/h)/(µmol photons)Brading, Patrick
25 Light saturationEkµmol/m2/sBrading, Patrick
26 Carbon/Chlorophyll a ratioC/Chl aBrading, Patrick[g/g]
27 Growth rateµ#/dayBrading, PatrickCalculated
28 Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
29 Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
30 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
31 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
32 Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
33 Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
34 Alkalinity, totalATµmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
35 Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
36 Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
5700 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


ID

Exp treat

Sal

Temp [°C]

PAR [µmol/m2/s]

pH
(Total scale, Ross Ultra pH el...)

AT [µmol/kg]
(Calculated using CO2SYS)

AT std e [±]

DIC [µmol/kg]
(TOC analyzer (Shimadzu))
10 
DIC std e [±]
11 
pCO2water_SST_wet [µatm]
(Calculated using CO2SYS)
12 
pCO2water_SST_wet std e [±]
13 
[HCO3]- [µmol/kg]
(Calculated using CO2SYS)
14 
[HCO3]- std e [±]
15 
[CO3]2- [µmol/kg]
(Calculated using CO2SYS)
16 
[CO3]2- std e [±]
17 
CO2 [µmol/kg]
(Calculated using CO2SYS)
18 
CO2 std e [±]
19 
Omega Cal
(Calculated using CO2SYS)
20 
Omega Cal std dev [±]
21 
Omega Arg
(Calculated using CO2SYS)
22 
Omega Arg std dev [±]
23 
Pmax O2/cell [fmol/#/h]
24 
alpha/cell [(fmol/#/h)/(µmol photons)]
25 
Ek [µmol/m2/s]
26 
C/Chl a
([g/g])
27 
µ [#/day]
(Calculated)
28 
CSC flag
(Calculated using seacarb afte...)
29 
CO2 [µmol/kg]
(Calculated using seacarb afte...)
30 
pCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)
31 
fCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)
32 
[HCO3]- [µmol/kg]
(Calculated using seacarb afte...)
33 
[CO3]2- [µmol/kg]
(Calculated using seacarb afte...)
34 
AT [µmol/kg]
(Calculated using seacarb afte...)
35 
Omega Arg
(Calculated using seacarb afte...)
36 
Omega Cal
(Calculated using seacarb afte...)
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.03538.751.82296.2075.470.45911.10402.02400.771804.71227.172360.293.625.48
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.03437.601.98220.5472.660.23911.10402.02400.771804.71227.172360.293.625.48
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.03485.262.43199.8780.380.18911.10402.02400.771804.71227.172360.293.625.48
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.0381.390.54911.10402.02400.771804.71227.172360.293.625.48
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.030.32911.10402.02400.771804.71227.172360.293.625.48
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.030.32911.10402.02400.771804.71227.172360.293.625.48
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.030.32911.10402.02400.771804.71227.172360.293.625.48
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.030.40911.10402.02400.771804.71227.172360.293.625.48
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.030.42911.10402.02400.771804.71227.172360.293.625.48
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.030.53911.10402.02400.771804.71227.172360.293.625.48
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.030.29911.10402.02400.771804.71227.172360.293.625.48
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.030.30911.10402.02400.771804.71227.172360.293.625.48
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.030.21911.10402.02400.771804.71227.172360.293.625.48
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.030.34911.10402.02400.771804.71227.172360.293.625.48
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.030.49911.10402.02400.771804.71227.172360.293.625.48
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.030.29911.10402.02400.771804.71227.172360.293.625.48
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.030.57911.10402.02400.771804.71227.172360.293.625.48
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.030.34911.10402.02400.771804.71227.172360.293.625.48
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.030.43911.10402.02400.771804.71227.172360.293.625.48
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.030.58911.10402.02400.771804.71227.172360.293.625.48
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.030.41911.10402.02400.771804.71227.172360.293.625.48
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.030.37911.10402.02400.771804.71227.172360.293.625.48
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.030.34911.10402.02400.771804.71227.172360.293.625.48
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.030.37911.10402.02400.771804.71227.172360.293.625.48
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.030.50911.10402.02400.771804.71227.172360.293.625.48
A1Ambient35.00026.000350.0008.052356.5517.422042.9815.78404.973.131808.1913.96223.621.7311.180.095.390.043.560.030.53911.10402.02400.771804.71227.172360.293.625.48
A1Elevated35.00026.000350.0007.792460.359.622279.209.14860.703.452111.918.47143.530.5823.760.103.460.012.290.01513.613.07167.38101.480.43923.61855.16852.502109.66145.942462.982.333.52
A1Elevated35.00026.000350.0007.792460.359.622279.209.14860.703.452111.918.47143.530.5823.760.103.460.012.290.01692.152.57269.28114.400.36923.61855.16852.502109.66145.942462.982.333.52
A1Elevated35.00026.000350.0007.792460.359.622279.209.14860.703.452111.918.47143.530.5823.760.103.460.012.290.01343.311.65208.1393.470.44923.61855.16852.502109.66145.942462.982.333.52
A1Elevated35.00026.000350.0007.792460.359.622279.209.14860.703.452111.918.47143.530.5823.760.103.460.012.290.010.44923.61855.16852.502109.66145.942462.982.333.52
A1Elevated35.00026.000350.0007.792460.359.622279.209.14860.703.452111.918.47143.530.5823.760.103.460.012.290.010.48923.61855.16852.502109.66145.942462.982.333.52
A1Elevated35.00026.000350.0007.792460.359.622279.209.14860.703.452111.918.47143.530.5823.760.103.460.012.290.010.22923.61855.16852.502109.66145.942462.982.333.52
A1Elevated35.00026.000350.0007.792460.359.622279.209.14860.703.452111.918.47143.530.5823.760.103.460.012.290.010.66923.61855.16852.502109.66145.942462.982.333.52
A1Elevated35.00026.000350.0007.792460.359.622279.209.14860.703.452111.918.47143.530.5823.760.103.460.012.290.010.33923.61855.16852.502109.66145.942462.982.333.52
A1Elevated35.00026.000350.0007.792460.359.622279.209.14860.703.452111.918.47143.530.5823.760.103.460.012.290.010.32923.61855.16852.502109.66145.942462.982.333.52
A1Elevated35.00026.000350.0007.792460.359.622279.209.14860.703.452111.918.47143.530.5823.760.103.460.012.290.010.18923.61855.16852.502109.66145.942462.982.333.52
A1Elevated35.00026.000350.0007.792460.359.622279.209.14860.703.452111.918.47143.530.5823.760.103.460.012.290.010.43923.61855.16852.502109.66145.942462.982.333.52
A1Elevated35.00026.000350.0007.792460.359.622279.209.14860.703.452111.918.47143.530.5823.760.103.460.012.290.010.26923.61855.16852.502109.66145.942462.982.333.52
A1Elevated35.00026.000350.0007.792460.359.622279.209.14860.703.452111.918.47143.530.5823.760.103.460.012.290.010.30923.61855.16852.502109.66145.942462.982.333.52
A1Elevated35.00026.000350.0007.792460.359.622279.209.14860.703.452111.918.47143.530.5823.760.103.460.012.290.010.42923.61855.16852.502109.66145.942462.982.333.52
A1Elevated35.00026.000350.0007.792460.359.622279.209.14860.703.452111.918.47143.530.5823.760.103.460.012.290.010.30923.61855.16852.502109.66145.942462.982.333.52
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.03452.263.58126.1869.200.28911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.03620.604.13150.3872.430.50911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.03672.713.51191.9182.600.16911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.0374.150.36911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.030.54911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.030.20911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.030.24911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.030.60911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.030.56911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.030.09911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.030.58911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.030.43911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.030.23911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.030.39911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.030.60911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.030.09911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.030.43911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.030.36911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.030.48911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.030.11911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.030.80911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.030.22911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.030.33911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.030.51911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.030.42911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.030.04911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.030.46911.46415.15413.861863.67234.602434.103.745.65
A2Ambient35.00026.000350.0008.052430.2318.772109.7317.00418.203.371867.2715.05230.921.8611.540.095.570.043.680.030.24911.46415.15413.861863.67234.602434.103.745.65
A2Elevated35.00026.000350.0007.792485.5116.642303.1015.81869.725.972134.0614.65145.031.0024.010.163.500.022.310.02950.526.51146.0292.480.34923.85864.13861.442131.78147.472488.162.353.55
A2Elevated35.00026.000350.0007.792485.5116.642303.1015.81869.725.972134.0614.65145.031.0024.010.163.500.022.310.02792.384.70168.56102.720.46923.85864.13861.442131.78147.472488.162.353.55
A2Elevated35.00026.000350.0007.792485.5116.642303.1015.81869.725.972134.0614.65145.031.0024.010.163.500.022.310.02942.508.65109.00137.490.54923.85864.13861.442131.78147.472488.162.353.55
A2Elevated35.00026.000350.0007.792485.5116.642303.1015.81869.725.972134.0614.65145.031.0024.010.163.500.022.310.020.70923.85864.13861.442131.78147.472488.162.353.55
A2Elevated35.00026.000350.0007.792485.5116.642303.1015.81869.725.972134.0614.65145.031.0024.010.163.500.022.310.020.27923.85864.13861.442131.78147.472488.162.353.55
A2Elevated35.00026.000350.0007.792485.5116.642303.1015.81869.725.972134.0614.65145.031.0024.010.163.500.022.310.020.35923.85864.13861.442131.78147.472488.162.353.55
A2Elevated35.00026.000350.0007.792485.5116.642303.1015.81869.725.972134.0614.65145.031.0024.010.163.500.022.310.020.72923.85864.13861.442131.78147.472488.162.353.55
A2Elevated35.00026.000350.0007.792485.5116.642303.1015.81869.725.972134.0614.65145.031.0024.010.163.500.022.310.020.15923.85864.13861.442131.78147.472488.162.353.55
A2Elevated35.00026.000350.0007.792485.5116.642303.1015.81869.725.972134.0614.65145.031.0024.010.163.500.022.310.020.51923.85864.13861.442131.78147.472488.162.353.55
A2Elevated35.00026.000350.0007.792485.5116.642303.1015.81869.725.972134.0614.65145.031.0024.010.163.500.022.310.020.18923.85864.13861.442131.78147.472488.162.353.55
A2Elevated35.00026.000350.0007.792485.5116.642303.1015.81869.725.972134.0614.65145.031.0024.010.163.500.022.310.020.38923.85864.13861.442131.78147.472488.162.353.55
A2Elevated35.00026.000350.0007.792485.5116.642303.1015.81869.725.972134.0614.65145.031.0024.010.163.500.022.310.020.38923.85864.13861.442131.78147.472488.162.353.55
A2Elevated35.00026.000350.0007.792485.5116.642303.1015.81869.725.972134.0614.65145.031.0024.010.163.500.022.310.020.44923.85864.13861.442131.78147.472488.162.353.55
A2Elevated35.00026.000350.0007.792485.5116.642303.1015.81869.725.972134.0614.65145.031.0024.010.163.500.022.310.020.53923.85864.13861.442131.78147.472488.162.353.55
A2Elevated35.00026.000350.0007.792485.5116.642303.1015.81869.725.972134.0614.65145.031.0024.010.163.500.022.310.020.55923.85864.13861.442131.78147.472488.162.353.55
A2Elevated35.00026.000350.0007.792485.5116.642303.1015.81869.725.972134.0614.65145.031.0024.010.163.500.022.310.020.31923.85864.13861.442131.78147.472488.162.353.55
A2Elevated35.00026.000350.0007.792485.5116.642303.1015.81869.725.972134.0614.65145.031.0024.010.163.500.022.310.020.76923.85864.13861.442131.78147.472488.162.353.55
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.02409.092.30177.86105.090.05911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.02464.451.60290.32129.990.68911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.02484.882.23217.02104.470.12911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.0291.780.33911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.020.12911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.020.55911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.020.16911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.020.22911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.020.44911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.020.51911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.020.32911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.020.32911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.020.77911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.020.27911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.020.46911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.020.21911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.020.61911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.020.42911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.020.45911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.020.14911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.020.27911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.020.48911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.020.37911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.020.51911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.020.58911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.020.54911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.020.43911.09401.79400.541803.69227.052359.023.625.47
A13Ambient35.00026.000350.0008.052355.2815.192041.8313.76404.742.731807.1712.18223.491.5111.170.085.390.043.560.020.63911.09401.79400.541803.69227.052359.023.625.47
A13Elevated35.00026.000350.0007.792588.3613.382400.8212.71906.624.802224.6111.78151.190.8025.030.133.650.022.410.01546.622.16253.2591.890.65924.87900.79897.992222.23153.722591.122.453.71
A13Elevated35.00026.000350.0007.792588.3613.382400.8212.71906.624.802224.6111.78151.190.8025.030.133.650.022.410.01414.981.87222.41103.220.43924.87900.79897.992222.23153.722591.122.453.71
A13Elevated35.00026.000350.0007.792588.3613.382400.8212.71906.624.802224.6111.78151.190.8025.030.133.650.022.410.01473.281.84257.65104.890.66924.87900.79897.992222.23153.722591.122.453.71
A13Elevated35.00026.000350.0007.792588.3613.382400.8212.71906.624.802224.6111.78151.190.8025.030.133.650.022.410.010.77924.87900.79897.992222.23153.722591.122.453.71
A13Elevated35.00026.000350.0007.792588.3613.382400.8212.71906.624.802224.6111.78151.190.8025.030.133.650.022.410.010.45924.87900.79897.992222.23153.722591.122.453.71
A13Elevated35.00026.000350.0007.792588.3613.382400.8212.71906.624.802224.6111.78151.190.8025.030.133.650.022.410.010.73924.87900.79897.992222.23153.722591.122.453.71
A13Elevated35.00026.000350.0007.792588.3613.382400.8212.71906.624.802224.6111.78151.190.8025.030.133.650.022.410.010.58924.87900.79897.992222.23153.722591.122.453.71
A13Elevated35.00026.000350.0007.792588.3613.382400.8212.71906.624.802224.6111.78151.190.8025.030.133.650.022.410.010.57924.87900.79897.992222.23153.722591.122.453.71
A13Elevated35.00026.000350.0007.792588.3613.382400.8212.71906.624.802224.6111.78151.190.8025.030.133.650.022.410.010.53924.87900.79897.992222.23153.722591.122.453.71
A13Elevated35.00026.000350.0007.792588.3613.382400.8212.71906.624.802224.6111.78151.190.8025.030.133.650.022.410.010.66924.87900.79897.992222.23153.722591.122.453.71
A13Elevated35.00026.000350.0007.792588.3613.382400.8212.71906.624.802224.6111.78151.190.8025.030.133.650.022.410.010.73924.87900.79897.992222.23153.722591.122.453.71
A13Elevated35.00026.000350.0007.792588.3613.382400.8212.71906.624.802224.6111.78151.190.8025.030.133.650.022.410.010.69924.87900.79897.992222.23153.722591.122.453.71
A13Elevated35.00026.000350.0007.792588.3613.382400.8212.71906.624.802224.6111.78151.190.8025.030.133.650.022.410.010.52924.87900.79897.992222.23153.722591.122.453.71
A13Elevated35.00026.000350.0007.792588.3613.382400.8212.71906.624.802224.6111.78151.190.8025.030.133.650.022.410.010.77924.87900.79897.992222.23153.722591.122.453.71
A13Elevated35.00026.000350.0007.792588.3613.382400.8212.71906.624.802224.6111.78151.190.8025.030.133.650.022.410.010.61924.87900.79897.992222.23153.722591.122.453.71
A13Elevated35.00026.000350.0007.792588.3613.382400.8212.71906.624.802224.6111.78151.190.8025.030.133.650.022.410.010.57924.87900.79897.992222.23153.722591.122.453.71
A13Elevated35.00026.000350.0007.792588.3613.382400.8212.71906.624.802224.6111.78151.190.8025.030.133.650.022.410.010.77924.87900.79897.992222.23153.722591.122.453.71
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.02338.980.90376.69108.530.41910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.02372.131.13327.93130.060.32910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.02262.240.90290.22130.800.55910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.02128.360.22910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.020.60910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.020.31910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.020.59910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.020.44910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.020.40910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.020.19910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.020.46910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.020.22910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.020.10910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.020.59910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.020.28910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.020.24910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.020.42910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.020.37910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.020.44910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.020.32910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.020.29910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.020.19910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.020.39910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.020.35910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.020.27910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.020.36910.91395.21393.981774.15223.332322.043.565.38
B1Ambient35.00026.000350.0008.052318.3615.092008.3913.67398.112.711777.5712.10219.831.5010.990.075.300.043.500.020.58910.91395.21393.981774.15223.332322.043.565.38
B1Elevated35.00026.000350.0007.792411.1412.362232.4411.74843.044.432068.5910.88140.580.7423.270.123.390.022.240.01394.081.62243.48169.740.45923.12837.62835.012066.38142.942413.712.283.45
B1Elevated35.00026.000350.0007.792411.1412.362232.4411.74843.044.432068.5910.88140.580.7423.270.123.390.022.240.01273.901.08253.38148.140.46923.12837.62835.012066.38142.942413.712.283.45
B1Elevated35.00026.000350.0007.792411.1412.362232.4411.74843.044.432068.5910.88140.580.7423.270.123.390.022.240.01318.341.03310.00182.360.36923.12837.62835.012066.38142.942413.712.283.45
B1Elevated35.00026.000350.0007.792411.1412.362232.4411.74843.044.432068.5910.88140.580.7423.270.123.390.022.240.010.37923.12837.62835.012066.38142.942413.712.283.45
B1Elevated35.00026.000350.0007.792411.1412.362232.4411.74843.044.432068.5910.88140.580.7423.270.123.390.022.240.010.33923.12837.62835.012066.38142.942413.712.283.45
B1Elevated35.00026.000350.0007.792411.1412.362232.4411.74843.044.432068.5910.88140.580.7423.270.123.390.022.240.010.60923.12837.62835.012066.38142.942413.712.283.45
B1Elevated35.00026.000350.0007.792411.1412.362232.4411.74843.044.432068.5910.88140.580.7423.270.123.390.022.240.010.53923.12837.62835.012066.38142.942413.712.283.45
B1Elevated35.00026.000350.0007.792411.1412.362232.4411.74843.044.432068.5910.88140.580.7423.270.123.390.022.240.010.34923.12837.62835.012066.38142.942413.712.283.45
B1Elevated35.00026.000350.0007.792411.1412.362232.4411.74843.044.432068.5910.88140.580.7423.270.123.390.022.240.010.49923.12837.62835.012066.38142.942413.712.283.45
B1Elevated35.00026.000350.0007.792411.1412.362232.4411.74843.044.432068.5910.88140.580.7423.270.123.390.022.240.010.33923.12837.62835.012066.38142.942413.712.283.45
B1Elevated35.00026.000350.0007.792411.1412.362232.4411.74843.044.432068.5910.88140.580.7423.270.123.390.022.240.010.31923.12837.62835.012066.38142.942413.712.283.45
B1Elevated35.00026.000350.0007.792411.1412.362232.4411.74843.044.432068.5910.88140.580.7423.270.123.390.022.240.010.36923.12837.62835.012066.38142.942413.712.283.45
B1Elevated35.00026.000350.0007.792411.1412.362232.4411.74843.044.432068.5910.88140.580.7423.270.123.390.022.240.010.34923.12837.62835.012066.38142.942413.712.283.45
B1Elevated35.00026.000350.0007.792411.1412.362232.4411.74843.044.432068.5910.88140.580.7423.270.123.390.022.240.010.31923.12837.62835.012066.38142.942413.712.283.45
B1Elevated35.00026.000350.0007.792411.1412.362232.4411.74843.044.432068.5910.88140.580.7423.270.123.390.022.240.010.30923.12837.62835.012066.38142.942413.712.283.45
B1Elevated35.00026.000350.0007.792411.1412.362232.4411.74843.044.432068.5910.88140.580.7423.270.123.390.022.240.010.34923.12837.62835.012066.38142.942413.712.283.45
B1Elevated35.00026.000350.0007.792411.1412.362232.4411.74843.044.432068.5910.88140.580.7423.270.123.390.022.240.010.31923.12837.62835.012066.38142.942413.712.283.45