/* DATA DESCRIPTION: Citation: Dueñas-Bohórquez, Adriana; Raitzsch, Markus; de Nooijer, Lennart Jan; Reichart, Gert-Jan (2011): Seawater carbonate chemistry and impacts of calcium and carbonate ion concentration on Mg and Sr incorporation in cultured benthic foraminifera (Ammonia tepida and Heterostegina depressa), 2011. PANGAEA, https://doi.org/10.1594/PANGAEA.770474, Supplement to: Dueñas-Bohórquez, A et al. (2011): Independent impacts of calcium and carbonate ion concentration on Mg and Sr incorporation in cultured benthic foraminifera. Marine Micropaleontology, 81(3-4), 122-130, https://doi.org/10.1016/j.marmicro.2011.08.002 Abstract: Laboratory culture experiments were conducted to determine effects of seawater carbonate ion concentration ([CO32-]), and thereby calcite saturation state, on Mg and Sr incorporation into calcite of two species of shallow-water benthic foraminifera: Ammonia tepida and Heterostegina depressa. Impact on Mg and Sr incorporation by increased seawater [CO32-] and thereby higher calcite saturation state, is absent in either species. Comparison to results from a similar culturing experiment, in which calcite saturation state was varied as a function of [Ca2+], reveals that saturation state affects incorporation of Mg and Sr through calcium- rather than carbonate availability. The similarity in response by both species is surprising since the average Mg/Ca ratio is ~ 70 times higher in H. depressa than in A. tepida. Furthermore, these results suggest that the ions involved in biomineralization (i.e. Ca2+ and DIC) are processed by separate cellular transport mechanisms. The similar response of Mg and Sr incorporation in this study suggests that only differences in the Ca2+ transport mechanism affect divalent cation partitioning. Keyword(s): Ammonia tepida; Benthos; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Coast and continental shelf; Foraminifera; Heterostegina depressa; Heterotrophic prokaryotes; Laboratory experiment; Mortality/Survival; North Atlantic; Single species; Temperate Project(s): European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS) (URI: https://eur-oceans.eu/) European Project on Ocean Acidification (EPOCA) (URI: https://en.wikipedia.org/wiki/European_Project_on_Ocean_Acidification) Ocean Acidification International Coordination Centre (OA-ICC) (URI: https://www.iaea.org/services/oa-icc/science-and-collaboration/data-access-and-management) Funding: Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification (URI: https://cordis.europa.eu/project/id/211384) Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis (URI: https://cordis.europa.eu/project/id/511106) Comment: In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). Parameter(s): Species (Species) * PI: Dueñas-Bohórquez, Adriana Identification (ID) * PI: Dueñas-Bohórquez, Adriana Salinity (Sal) * PI: Dueñas-Bohórquez, Adriana Salinity, standard deviation [±] (Sal std dev) * PI: Dueñas-Bohórquez, Adriana Temperature, water [°C] (Temp) * PI: Dueñas-Bohórquez, Adriana Temperature, standard deviation [±] (T std dev) * PI: Dueñas-Bohórquez, Adriana Alkalinity, total [µmol/kg] (AT) * PI: Dueñas-Bohórquez, Adriana * METHOD/DEVICE: Measured Alkalinity, total, standard deviation [±] (AT std dev) * PI: Dueñas-Bohórquez, Adriana Carbon, inorganic, dissolved [µmol/kg] (DIC) * PI: Dueñas-Bohórquez, Adriana * METHOD/DEVICE: Measured Carbon, inorganic, dissolved, standard deviation [±] (DIC std dev) * PI: Dueñas-Bohórquez, Adriana pH (pH) * PI: Dueñas-Bohórquez, Adriana * METHOD/DEVICE: Calculated using CO2SYS (URI: http://cdiac.ornl.gov/oceans/co2rprt.html) pH, standard deviation [±] (pH std dev) * PI: Dueñas-Bohórquez, Adriana Carbonate ion [µmol/kg] ([CO3]2-) * PI: Dueñas-Bohórquez, Adriana * METHOD/DEVICE: Calculated using CO2SYS (URI: http://cdiac.ornl.gov/oceans/co2rprt.html) Carbonate ion, standard deviation [±] ([CO3]2- std dev) * PI: Dueñas-Bohórquez, Adriana Calcite saturation state (Omega Cal) * PI: Dueñas-Bohórquez, Adriana * METHOD/DEVICE: Calculated using CO2SYS (URI: http://cdiac.ornl.gov/oceans/co2rprt.html) Calcite saturation state, standard deviation [±] (Omega Cal std dev) * PI: Dueñas-Bohórquez, Adriana Number of specimens [#] (No spec) * PI: Dueñas-Bohórquez, Adriana * COMMENT: Beginning of experiment Number of specimens [#] (No spec) * PI: Dueñas-Bohórquez, Adriana * COMMENT: Living at the end of experiment Number of specimens [#] (No spec) * PI: Dueñas-Bohórquez, Adriana * COMMENT: Living juveniles at the end of experiment Number of specimens [#] (No spec) * PI: Dueñas-Bohórquez, Adriana * COMMENT: Specimens analysed Ammonia tepida, chambers added [#] (A. tepida chambers) * PI: Dueñas-Bohórquez, Adriana Ammonia tepida, chambers added per individual [#/#] (A. tepida chambers/ind) * PI: Dueñas-Bohórquez, Adriana Rows of chambers added [#] (Rows chamb) * PI: Dueñas-Bohórquez, Adriana * COMMENT: of Heterostegian depressa Rows of chambers added per individual [#/#] (Rows chamb/ind) * PI: Dueñas-Bohórquez, Adriana * COMMENT: of Heterostegian depressa Replicates [#] (Repl) * PI: Dueñas-Bohórquez, Adriana Ammonia tepida, magnesium/calcium ratio [mmol/mol] (A. tepida Mg/Ca) * PI: Dueñas-Bohórquez, Adriana Ammonia tepida, magnesium/calcium ratio, standard deviation [±] (A. tepida Mg/Ca std dev) * PI: Dueñas-Bohórquez, Adriana Heterostegina depressa, magnesium/calcium ratio [mmol/mol] (H. depressa Mg/Ca) * PI: Dueñas-Bohórquez, Adriana Heterostegina depressa, magnesium/calcium ratio, standard deviation [±] (H. depressa Mg/Ca std dev) * PI: Dueñas-Bohórquez, Adriana Ammonia tepida, strontium/calcium ratio [mmol/mol] (A. tepida Sr/Ca) * PI: Dueñas-Bohórquez, Adriana Ammonia tepida, strontium/calcium ratio, standard deviation [±] (A. tepida Sr/Ca std dev) * PI: Dueñas-Bohórquez, Adriana Heterostegina depressa, strontium/calcium ratio [mmol/mol] (H. depressa Sr/Ca) * PI: Dueñas-Bohórquez, Adriana Heterostegina depressa, strontium/calcium ratio, standard deviation [±] (H. depressa Sr/Ca std dev) * PI: Dueñas-Bohórquez, Adriana Carbonate system computation flag (CSC flag) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) pH (pH) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: Total scale Carbon dioxide [µmol/kg] (CO2) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) [µatm] (pCO2water_SST_wet) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Fugacity of carbon dioxide (water) at sea surface temperature (wet air) [µatm] (fCO2water_SST_wet) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Bicarbonate ion [µmol/kg] ([HCO3]-) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Carbonate ion [µmol/kg] ([CO3]2-) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Aragonite saturation state (Omega Arg) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Calcite saturation state (Omega Cal) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) License: Creative Commons Attribution 3.0 Unported (CC-BY-3.0) (URI: https://creativecommons.org/licenses/by/3.0/) Status: Curation Level: Enhanced curation (URI: https://wiki.pangaea.de/wiki/Curation_levels) Size: 282 data points */ Species ID Sal Sal std dev [±] Temp [°C] T std dev [±] AT [µmol/kg] (Measured) AT std dev [±] DIC [µmol/kg] (Measured) DIC std dev [±] pH (Calculated using CO2SYS) pH std dev [±] [CO3]2- [µmol/kg] (Calculated using CO2SYS) [CO3]2- std dev [±] Omega Cal (Calculated using CO2SYS) Omega Cal std dev [±] No spec [#] (Beginning of experiment) No spec [#] (Living at the end of experiment) No spec [#] (Living juveniles at the end o...) No spec [#] (Specimens analysed) A. tepida chambers [#] A. tepida chambers/ind [#/#] Rows chamb [#] (of Heterostegian depressa) Rows chamb/ind [#/#] (of Heterostegian depressa) Repl [#] A. tepida Mg/Ca [mmol/mol] A. tepida Mg/Ca std dev [±] H. depressa Mg/Ca [mmol/mol] H. depressa Mg/Ca std dev [±] A. tepida Sr/Ca [mmol/mol] A. tepida Sr/Ca std dev [±] H. depressa Sr/Ca [mmol/mol] H. depressa Sr/Ca std dev [±] CSC flag (Calculated using seacarb afte...) pH (Total scale, Calculated using...) CO2 [µmol/kg] (Calculated using seacarb afte...) pCO2water_SST_wet [µatm] (Calculated using seacarb afte...) fCO2water_SST_wet [µatm] (Calculated using seacarb afte...) [HCO3]- [µmol/kg] (Calculated using seacarb afte...) [CO3]2- [µmol/kg] (Calculated using seacarb afte...) Omega Arg (Calculated using seacarb afte...) Omega Cal (Calculated using seacarb afte...) Ammonia tepida 1 35 0.1 18 0.5 2236 5 2074 36 8.00 0.07 124 22 3 0.5 31 82 9 17 2.00 9 1.63 0.06 1.47 0.04 15 7.88 20.55 601.450 599.390 1930.570 122.880 1.90 2.93 Ammonia tepida 2 35 0.3 18 0.5 2340 28 2137 74 8.08 0.10 158 43 4 0.8 30 21 >50 8 32 4.00 8 1.69 0.03 1.35 0.03 15 7.96 17.44 510.490 508.730 1968.960 150.590 2.32 3.59 Ammonia tepida 3 35 0.1 18 0.5 2500 11 2076 40 8.43 0.05 298 31 7 0.7 34 34 >50 10 28 2.80 10 1.87 0.08 1.39 0.04 15 8.31 7.06 206.520 205.810 1768.600 300.340 4.64 7.17 Ammonia tepida 4 35 0.1 18 0.5 2700 12 2084 26 8.63 0.02 432 22 10 0.5 36 84 11 37 3.40 11 2.00 0.07 1.50 0.04 15 8.51 4.11 120.380 119.960 1637.940 441.950 6.82 10.55 Heterostegina depressa 1 35 0.1 24 0.5 2250 5 2080 25 7.95 0.05 130 13 3 0.3 30 30 9 10 1.10 9 135.00 4.00 2.50 0.07 15 7.81 21.31 734.000 731.660 1928.230 130.460 2.06 3.13 Heterostegina depressa 2 35 0.1 24 0.5 2500 40 2074 25 8.35 0.02 290 37 7 0.9 31 31 11 11 1.00 11 132.00 3.00 2.56 0.06 15 8.22 7.64 263.020 262.180 1762.270 304.100 4.80 7.31 Heterostegina depressa 3 35 0.1 24 0.5 2550 74 2098 29 8.38 0.04 324 63 8 1.5 35 35 12 14 1.20 12 134.00 3.00 2.42 0.04 15 8.25 7.21 248.270 247.480 1766.920 323.870 5.11 7.78 Heterostegina depressa 4 35 0.1 24 0.5 2800 40 2114 48 8.61 0.02 465 34 11 0.8 30 30 5 5 1.00 5 144.00 6.00 2.54 0.10 15 8.47 3.90 134.370 133.940 1612.030 498.070 7.86 11.97