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Wong, Kevin K W; Lane, Ackley Charles; Leung, Priscilla TY; Thlyagarajan, V (2011): Seawater carbonate chemistry and protein sports of barnicle Balanus amphitrite during experiments, 2011 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.770091, Supplement to: Wong, KKW et al. (2011): Response of larval barnacle proteome to CO2-driven seawater acidification. Comparative Biochemistry and Physiology Part D: Genomics & Proteomics, 6(3), 310-321, https://doi.org/10.1016/j.cbd.2011.07.001

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Abstract:
The majority of benthic marine invertebrates have a complex life cycle, during which the pelagic larvae select a suitable substrate, attach to it, and then metamorphose into benthic adults. Anthropogenic ocean acidification (OA) is postulated to affect larval metamorphic success through an altered protein expression pattern (proteome structure) and post-translational modifications. To test this hypothesis, larvae of an economically and ecologically important barnacle species Balanus amphitrite, were cultured from nauplius to the cyprid stage in the present (control) and in the projected elevated concentrations of CO2 for the year 2100 (the OA treatment). Cyprid response to OA was analyzed at the total proteome level as well as two protein post-translational modification (phosphorylation and glycosylation) levels using a 2-DE based proteomic approach. The cyprid proteome showed OA-driven changes. Proteins that were differentially up or down regulated by OA come from three major groups, namely those related to energy-metabolism, respiration, and molecular chaperones, illustrating a potential strategy that the barnacle larvae may employ to tolerate OA stress. The differentially expressed proteins were tentatively identified as OA-responsive, effectively creating unique protein expression signatures for OA scenario of 2100. This study showed the promise of using a sentinel and non-model species to examine the impact of OA at the proteome level.
Keyword(s):
Animalia; Arthropoda; Balanus amphitrite; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Gene expression (incl. proteomics); Laboratory experiment; North Pacific; Pelagos; Single species; Tropical; Zooplankton
Project(s):
European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)
European Project on Ocean Acidification (EPOCA)
Ocean Acidification International Coordination Centre (OA-ICC)
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 Experimental treatmentExp treatWong, Kevin K W
2 IdentificationIDWong, Kevin K W
3 SalinitySalWong, Kevin K W
4 Salinity, standard errorSal std e±Wong, Kevin K W
5 Temperature, waterTemp°CWong, Kevin K W
6 Temperature, water, standard errorT std e±Wong, Kevin K W
7 Alkalinity, totalATmmol(eq)/lWong, Kevin K WAlkalinity, Gran titration (Gran, 1950)
8 Alkalinity, total, standard errorAT std e±Wong, Kevin K W
9 pHpHWong, Kevin K WpH meter (Metrohm electrodes)Total scale; H+ ion concentration in µmol/l
10 pH, standard errorpH std e±Wong, Kevin K W
11 Alkalinity, totalATµmol/kgWong, Kevin K WCalculated
12 pHpHWong, Kevin K WCalculatedTotal scale; H+ ion concentration in µmol/kg
13 Carbon, inorganic, dissolvedDICµmol/lWong, Kevin K WCalculated using CO2SYS
14 Carbon, inorganic, dissolved, standard errorDIC std e±Wong, Kevin K W
15 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmWong, Kevin K WCalculated using CO2SYS
16 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard errorpCO2water_SST_wet std e±Wong, Kevin K W
17 Bicarbonate[HCO3]-mmol/lWong, Kevin K WCalculated using CO2SYS
18 Carbonate ion, standard error[CO3]2- std e±Wong, Kevin K W
19 Carbonate ion[CO3]2-µmol/lWong, Kevin K WCalculated using CO2SYS
20 Bicarbonate ion, standard error[HCO3]- std e±Wong, Kevin K W
21 Carbon dioxide, totalTCO2µmol/lWong, Kevin K WCalculated using CO2SYS
22 Carbon dioxide, standard errorCO2 std e±Wong, Kevin K W
23 Calcite saturation stateOmega CalWong, Kevin K WCalculated using CO2SYS
24 Calcite saturation state, standard deviationOmega Cal std dev±Wong, Kevin K W
25 Sample IDSample IDWong, Kevin K W
26 Protein spots, totalProtein spots#Wong, Kevin K W
27 Phosphoprotein spotsphosphoprotein spots#Wong, Kevin K W
28 Glycoprotein spotsglycoprotein spots#Wong, Kevin K W
29 Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
30 Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
31 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
32 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
33 Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
34 Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
35 Carbon, inorganic, dissolvedDICµmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
36 Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
37 Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
354 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
Exp treat		2 
ID		3 
Sal		4 
Sal std e [±]		5 
Temp [°C]		6 
T std e [±]		7 
AT [mmol(eq)/l]
(Alkalinity, Gran titration (G...)		8 
AT std e [±]		9 
pH
(Total scale; H+ ion concentra...)		10 
pH std e [±]		11 
AT [µmol/kg]
(Calculated)		12 
pH
(Total scale; H+ ion concentra...)		13 
DIC [µmol/l]
(Calculated using CO2SYS)		14 
DIC std e [±]		15 
pCO2water_SST_wet [µatm]
(Calculated using CO2SYS)		16 
pCO2water_SST_wet std e [±]		17 
[HCO3]- [mmol/l]
(Calculated using CO2SYS)		18 
[CO3]2- std e [±]		19 
[CO3]2- [µmol/l]
(Calculated using CO2SYS)		20 
[HCO3]- std e [±]		21 
TCO2 [µmol/l]
(Calculated using CO2SYS)		22 
CO2 std e [±]		23 
Omega Cal
(Calculated using CO2SYS)		24 
Omega Cal std dev [±]		25 
Sample ID		26 
Protein spots [#]		27 
phosphoprotein spots [#]		28 
glycoprotein spots [#]		29 
CSC flag
(Calculated using seacarb afte...)		30 
CO2 [µmol/kg]
(Calculated using seacarb afte...)		31 
pCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		32 
fCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		33 
[HCO3]- [µmol/kg]
(Calculated using seacarb afte...)		34 
[CO3]2- [µmol/kg]
(Calculated using seacarb afte...)		35 
DIC [µmol/kg]
(Calculated using seacarb afte...)		36 
Omega Arg
(Calculated using seacarb afte...)		37 
Omega Cal
(Calculated using seacarb afte...)
ControlT036.00.228.40.62.39600.02008.100.012342.097.921986.010.0340.07.01682.09.0296.010.08.80.27.090.22814.69566.69564.971865.68194.322074.693.114.66
ControlTf34.00.029.90.32.18300.02108.020.012138.077.851853.016.0394.014.01605.015.0238.07.09.90.45.830.18816.02633.82631.941751.01156.501923.542.563.84
Controlaverage35.00.129.20.52.28950.02058.100.002240.227.891919.513.0367.010.51643.512.0267.08.59.40.36.500.20CON-R1567193130815.13591.80590.031805.46176.591997.182.864.28
Controlaverage35.00.129.20.52.28950.02058.100.002240.227.891919.513.0367.010.51643.512.0267.08.59.40.36.500.20CON-R2566193131815.13591.80590.031805.46176.591997.182.864.28
Controlaverage35.00.129.20.52.28950.02058.100.002240.227.891919.513.0367.010.51643.512.0267.08.59.40.36.500.20CON-R3566194130815.13591.80590.031805.46176.591997.182.864.28
pH=7.6T036.10.628.00.62.39700.03007.580.022342.597.412279.033.01428.074.02130.032.0111.01.037.41.72.660.03855.742131.372124.902173.7669.122298.611.101.66
pH=7.6Tf34.00.329.40.12.18300.00507.620.012137.727.462057.04.01189.035.01916.06.0110.04.030.41.02.700.08844.521740.811735.611964.5270.262079.291.151.72
pH=7.6average35.10.528.70.42.29000.01757.600.002240.187.432168.018.51308.554.52023.019.0110.52.533.91.42.700.107.6-R1567192130850.451951.471945.602070.5669.122190.131.121.67
pH=7.6average35.10.528.70.42.29000.01757.600.002240.187.432168.018.51308.554.52023.019.0110.52.533.91.42.700.107.6-R2566192130850.451951.471945.602070.5669.122190.131.121.67
pH=7.6average35.10.528.70.42.29000.01757.600.002240.187.432168.018.51308.554.52023.019.0110.52.533.91.42.700.107.6-R3566192130850.451951.471945.602070.5669.122190.131.121.67