Citation:

ame01520

Name: Seawater carbonate chemistry, POC, PIC, TPC, SPM, N, TEP and growth rate during experiments with coccolithophores Emiliania huxleyi (AC472), Calcidiscus leptoporus (AC370) and Syracosphaera pulchra (AC418) during experiments, 2011
Published: 2011-09-23
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcidiscus leptoporus; Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Mediterranean Sea; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Syracosphaera pulchra; Temperature

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Abstract:

The response of three coccolithophores (Emiliania huxleyi, Calcidiscus leptoporus and Syracosphaera pulchra) to elevated partial pressure (pCO2) of carbon dioxide was investigated in batch cultures. For the first time, we also report on the response of the non calcifying (haploid) life stage of these three species. The growth rate, cell size, inorganic (PIC) and organic carbon (POC) of both life stages were measured at two different pCO2 (400and 760 ppm) and their organic and inorganic carbon production calculated. The two lifestages within the same species generally exhibited a similar response to elevated pCO2, theresponse of the haploid stage being often more pronounced than that of the diploid stage. Thegrowth rate was consistently higher at higher pCO2 but the response of other processes varied among species. The calcification rate of C. leptoporus and of S. pulchra did not change at elevated pCO2 while increased in E. huxleyi. The POC production as well as the cell size of both life stages of S. pulchra and of the haploid stage of E. huxleyi markedly decreased at elevated pCO2. It remained unaltered in the diploid stage of E. huxleyi and C. leptoporus and increased in the haploid stage of the latter. The PIC:POC ratio increased in E. huxleyi and was constant in C. leptoporus and S. pulchra. These results suggest that the non-calcifying stage, is more responsive than the calcifying stage and that the most versatile genera will proliferate in a more acidic ocean rather than all coccolithophores will decline.

Keyword(s):

Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcidiscus leptoporus; Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Mediterranean Sea; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Syracosphaera pulchra; Temperature

Project(s):

European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)

European Project on Ocean Acidification (EPOCA)

Ocean Acidification International Coordination Centre (OA-ICC)

Funding:

Seventh Framework Programme (FP7), grant/award no. 211384 : European Project on Ocean Acidification

Sixth Framework Programme (FP6), grant/award no. 511106 : European network of excellence for Ocean Ecosystems Analysis

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Fiorini, Sarah
2	Sample ID	Sample ID		Fiorini, Sarah
3	Experimental treatment	Exp treat		Fiorini, Sarah
4	Salinity	Sal		Fiorini, Sarah
5	Temperature, water	Temp	°C	Fiorini, Sarah
6	Radiation, photosynthetically active	PAR	µmol/m²/s	Fiorini, Sarah
7	Light:Dark cycle	L:D	hh:hh	Fiorini, Sarah	Measured
8	Calcite saturation state	Omega Cal		Fiorini, Sarah	Calculated using seacarb
9	Emiliania huxleyi	E. huxleyi	#/l	Fiorini, Sarah	Coulter Counter (Beckman Coulter)
10	Calcidiscus leptoporus	C. leptoporus	#/l	Fiorini, Sarah	Coulter Counter (Beckman Coulter)
11	Syracosphaera pulchra	S. pulchra	#/l	Fiorini, Sarah	Coulter Counter (Beckman Coulter)
12	Growth rate	µ	#/day	Fiorini, Sarah	Calculated, see reference(s)
13	Carbon, inorganic, dissolved	DIC	µmol/kg	Fiorini, Sarah	Calculated using seacarb	T0
14	Carbon, inorganic, dissolved	DIC	µmol/kg	Fiorini, Sarah	Calculated using seacarb	Tfin
15	Carbon, inorganic, dissolved	DIC	µmol/kg	Fiorini, Sarah	Calculated	average
16	Alkalinity, total	AT	µmol/kg	Fiorini, Sarah	Alkalinity, Gran titration (Gran, 1950)	T0
17	Alkalinity, total	AT	µmol/kg	Fiorini, Sarah	Alkalinity, Gran titration (Gran, 1950)	Tfin
18	Alkalinity, total	AT	µmol/kg	Fiorini, Sarah	Calculated	average
19	Carbon dioxide	CO2	µmol/kg	Fiorini, Sarah	Calculated using seacarb	T0
20	Carbon dioxide	CO2	µmol/kg	Fiorini, Sarah	Calculated using seacarb	Tfin
21	pH	pH		Fiorini, Sarah	pH meter (Metrohm electrodes)	T0; Total scale
22	pH	pH		Fiorini, Sarah	pH meter (Metrohm electrodes)	Tfin; Total scale
23	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Fiorini, Sarah	Calculated using seacarb	T0
24	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Fiorini, Sarah	Calculated using seacarb	Tfin
25	Carbonate ion	[CO3]2-	µmol/kg	Fiorini, Sarah	Calculated using seacarb	T0
26	Carbonate ion	[CO3]2-	µmol/kg	Fiorini, Sarah	Calculated using seacarb	Tfin
27	Suspended particulate matter	SPM	mg/l	Fiorini, Sarah
28	Total particulate carbon production per cell	TPC prod/cell	pmol/#/day	Fiorini, Sarah	Mass spectrometer Thermo Electron Flash EA 1122 Analyzer
29	Carbon, total, particulate	TPC	µg/l	Fiorini, Sarah	Mass spectrometer Thermo Electron Flash EA 1122 Analyzer
30	Total particulate carbon per cell	TPC/cell	pg/#	Fiorini, Sarah	Mass spectrometer Thermo Electron Flash EA 1122 Analyzer
31	Carbon, organic, particulate	POC	%	Fiorini, Sarah	Mass spectrometer Thermo Electron Flash EA 1122 Analyzer
32	Carbon, organic, particulate	POC	µg/l	Fiorini, Sarah	Mass spectrometer Thermo Electron Flash EA 1122 Analyzer
33	Carbon, organic, particulate, per cell	POC/cell	pg/#	Fiorini, Sarah	Mass spectrometer Thermo Electron Flash EA 1122 Analyzer
34	Carbon, inorganic, particulate, per cell	PIC/cell	pg/#	Fiorini, Sarah	Calculated
35	Nitrogen, total	TN	%	Fiorini, Sarah	Skalar AutoAnalyser
36	Nitrogen	N	µg/l	Fiorini, Sarah	Skalar AutoAnalyser
37	Nitrogen per cell	N/cell	pg/#	Fiorini, Sarah	Skalar AutoAnalyser	Unit was N/# before, not sure if pg/# is correct, please be careful!
38	Carbonate system computation flag	CSC flag		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
39	pH	pH		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)	Total scale
40	Carbon dioxide	CO2	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
41	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
42	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
43	Bicarbonate ion	[HCO3]-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
44	Carbonate ion	[CO3]2-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
45	Aragonite saturation state	Omega Arg		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
46	Calcite saturation state	Omega Cal		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
47	Transparent exopolymer particles	TEP	ml	Pedrotti, Maria Luiza
48	Transparent exopolymer particles	TEP	µg C/l	Pedrotti, Maria Luiza
49	Transparent exopolymer particles per cell	TEP/cell	pg/#	Pedrotti, Maria Luiza

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1536 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 Sample ID	3 Exp treat	4 Sal	5 Temp [°C]	6 PAR [µmol/m²/s]	7 L:D [hh:hh] (Measured)	8 Omega Cal (Calculated using seacarb)	9 E. huxleyi [#/l] (Coulter Counter (Beckman Coul...)	10 C. leptoporus [#/l] (Coulter Counter (Beckman Coul...)	11 S. pulchra [#/l] (Coulter Counter (Beckman Coul...)	12 µ [#/day] (Calculated, see reference(s))	13 DIC [µmol/kg] (T0, Calculated using seacarb)	14 DIC [µmol/kg] (Tfin, Calculated using seacarb)	15 DIC [µmol/kg] (average, Calculated)	16 AT [µmol/kg] (T0, Alkalinity, Gran titratio...)	17 AT [µmol/kg] (Tfin, Alkalinity, Gran titrat...)	18 AT [µmol/kg] (average, Calculated)	19 CO2 [µmol/kg] (T0, Calculated using seacarb)	20 CO2 [µmol/kg] (Tfin, Calculated using seacarb)	21 pH (T0; Total scale, pH meter (Me...)	22 pH (Tfin; Total scale, pH meter (...)	23 pCO2water_SST_wet [µatm] (T0, Calculated using seacarb)	24 pCO2water_SST_wet [µatm] (Tfin, Calculated using seacarb)	25 [CO3]2- [µmol/kg] (T0, Calculated using seacarb)	26 [CO3]2- [µmol/kg] (Tfin, Calculated using seacarb)	27 SPM [mg/l]	28 TPC prod/cell [pmol/#/day] (Mass spectrometer Thermo Elec...)	29 TPC [µg/l] (Mass spectrometer Thermo Elec...)	30 TPC/cell [pg/#] (Mass spectrometer Thermo Elec...)	31 POC [%] (Mass spectrometer Thermo Elec...)	32 POC [µg/l] (Mass spectrometer Thermo Elec...)	33 POC/cell [pg/#] (Mass spectrometer Thermo Elec...)	34 PIC/cell [pg/#] (Calculated)	35 TN [%] (Skalar AutoAnalyser)	36 N [µg/l] (Skalar AutoAnalyser)	37 N/cell [pg/#] (Unit was N/# before, not sure...)	38 CSC flag (Calculated using seacarb afte...)	39 pH (Total scale, Calculated using...)	40 CO2 [µmol/kg] (Calculated using seacarb afte...)	41 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	42 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	43 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	44 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	45 Omega Arg (Calculated using seacarb afte...)	46 Omega Cal (Calculated using seacarb afte...)	47 TEP [ml]	48 TEP [µg C/l]	49 TEP/cell [pg/#]
Emiliania huxleyi	diploid A	pCO2=400	33.6	19	160	12:12	4.4	45947000			0.936	1984.28	1989.07	1982.67	2230.7	2250.0	2236.1	13.4	12.5	8.04	8.07	403	378	181.0	177.0	16.00	7.06	1.13	24.66	4.54	728.97	15.87	8.79	0.74	119	2.60	15	8.08	11.97	357.76	356.54	1790.23	180.47	2.82	4.35	1687	58.8	1.28
Emiliania huxleyi	diploid B	pCO2=400	33.6	19	160	12:12		51684000			0.957		1986.20			2248.7					8.07		375			18.90	6.67	1.25	24.17	4.26	798.66	15.45	8.71	0.70	131	2.54										1698	62.6	1.21
Emiliania huxleyi	diploid C	pCO2=400	33.6	19	160	12:12		47170000			0.952		1967.91			2226.0					8.06		376			19.50	5.99	1.16	24.65	3.96	768.07	16.28	8.37	0.67	129	2.74										2477	71.2	1.51
Emiliania huxleyi	haploid A	pCO2=400	33.6	19	160	12:12	4.4	33533000			0.929	1984.28	2030.09	1995.54	2230.7	2332.0	2269.2	13.4	10.8	8.04	8.13	403	332	181.0	204.0	19.70	4.19	0.83	24.71	4.18	824.30	24.58		0.70	137	4.09	15	8.11	11.10	331.75	330.63	1789.89	194.54	3.04	4.69	1069	10.8	0.32
Emiliania huxleyi	haploid B	pCO2=400	33.6	19	160	12:12		37998000			0.920		2008.01			2310.0					8.13		325			16.50	4.88	0.80	21.05	4.79	785.80	20.68		0.80	131	3.46										1542	15.6	0.41
Emiliania huxleyi	haploid C	pCO2=400	33.6	19	160	12:12		39742000			0.923		1982.32			2281.3					8.13		324			18.60	4.42	0.82	20.63	4.37	811.41	20.42		0.76	141	3.56										1079	10.9	0.27
Emiliania huxleyi	diploid A	pCO2=760	33.6	19	160	12:12	2.7	32935000			1.001	2100.50	2073.57	2086.60	2223.8	2216.0	2218.9	25.8	24.3	7.80	7.81	774	745	113.0	107.0	20.70	4.06	0.84	25.37	2.40	494.40	15.01	10.36	0.40	83	2.51	15	7.81	24.26	725.01	722.56	1956.03	106.31	1.66	2.56	3721	17.0	0.52
Emiliania huxleyi	diploid B	pCO2=760	33.6	19	160	12:12		31144000			0.979		2072.73			2213.3					7.80		748			22.50	3.60	0.81	25.98	2.20	493.56	15.85	10.13	0.43	96	3.07										3622	18.0	0.58
Emiliania huxleyi	diploid C	pCO2=760	33.6	19	160	12:12		35518000			0.974		2071.78			2213.0					7.81		742			21.90	3.93	0.86	24.20	2.23	488.23	13.75	10.45	0.44	96	2.69										3921	11.4	0.32
Emiliania huxleyi	haploid A	pCO2=760	33.6	19	160	12:12	2.7	25782000			0.943	2100.50	2107.15	2099.21	2223.8	2274.0	2243.0	25.8	21.8	7.80	7.86	774	664	113.0	121.0	20.00	2.10	0.42	16.30	2.08	415.03	16.10		0.41	83	3.20	15	7.84	22.88	683.72	681.40	1962.81	113.52	1.77	2.74	1104	3.2	0.13
Emiliania huxleyi	haploid B	pCO2=760	33.6	19	160	12:12		23952000			0.937		2104.69			2268.5					7.85		674			20.40	2.04	0.42	17.40	2.00	408.58	17.06		0.41	84	3.50										2149	6.3	0.26
Emiliania huxleyi	haploid C	pCO2=760	33.6	19	160	12:12		23557000			0.935		2081.90			2244.0					7.85		665			20.40	2.16	0.44	18.50	2.14	435.50	18.49		0.45	91	3.85										7200	9.8	0.42
Calcidiscus leptoporus	diploid A	pCO2=400	33.6	19	160	12:12	4.5		5705000		0.341	1966.59	1887.96	1929.82	2221.0	2078.7	2151.1	12.8	16.1	8.05	7.94	390	486	186.0	129.0	21.49	4.58	0.98	171.41	1.94	412.39	72.28	99.12	0.40	85	14.93	15	8.03	13.25	395.94	394.60	1759.12	157.45	2.46	3.80	1143	86.0	15.08
Calcidiscus leptoporus	diploid B	pCO2=400	33.6	19	160	12:12			4708000		0.332		1888.77			2074.0					7.93		498			19.12	5.27	1.00	213.40	2.07	395.43	83.99	129.41	0.41	79	16.77										1375	109.7	23.30
Calcidiscus leptoporus	diploid C	pCO2=400	33.6	19	160	12:12			4769000		0.317		1902.46			2091.0					7.94		492			22.26	4.32	0.96	201.73	1.93	429.61	90.08	111.65	0.37	81	17.08										1163	98.4	20.62
Calcidiscus leptoporus	haploid A	pCO2=400	33.6	19	160	12:12	4.5		9987000		0.531	1966.59	1942.45	1951.71	2221.0	2206.3	2210.1	12.8	11.9	8.05	8.08	390	355	186.0	177.0	17.51	3.81	0.67	66.92	3.73	654.28	65.51		0.45	78	7.84	15	8.09	11.40	340.70	339.55	1757.64	182.67	2.85	4.41	1143	71.8	7.19
Calcidiscus leptoporus	haploid B	pCO2=400	33.6	19	160	12:12			8752000		0.549		1935.76			2192.3					8.07		368			15.67	2.36	0.37	42.96	2.36	369.61	42.23		0.42	65	7.44										1375	59.1	6.76
Calcidiscus leptoporus	haploid C	pCO2=400	33.6	19	160	12:12			9064000		0.538		1932.28			2198.7					8.08		356			15.77	2.53	0.39	43.83	2.52	394.53	43.53		0.42	67	7.35										1163	91.3	10.07
Calcidiscus leptoporus	diploid A	pCO2=760	33.6	19	160	12:12	2.9		6064000		0.416	2133.07	2152.97	2135.78	2278.0	2265.0	2263.5	25.5	30.9	7.80	7.71	772	962	119.0	91.0	21.20	4.94	1.05	172.90	2.33	492.91	81.28	91.62	0.51	108	17.74	15	7.80	25.83	771.83	769.22	2005.02	104.93	1.64	2.53	2325	75.5	12.46
Calcidiscus leptoporus	diploid B	pCO2=760	33.6	19	160	12:12			6260000		0.428		2124.00			2233.0					7.71		962			20.67	5.48	1.13	180.91	2.45	507.42	81.06	99.85	0.52	108	17.22										5048	68.6	10.96
Calcidiscus leptoporus	haploid A	pCO2=760	33.6	19	160	12:12	2.9		6250000		0.634	2133.07	2201.16	2173.34	2278.0	2373.7	2334.2	25.5	22.6	7.80	7.86	772	700	119.0	131.0	5.67	6.11	0.35	55.52	6.09	344.57	55.34		1.02	58	9.24	15	7.87	21.92	655.08	652.86	2025.28	126.14	1.97	3.04	2852	31.7	5.10
Calcidiscus leptoporus	haploid B	pCO2=760	33.6	19	160	12:12			6012000		0.624		2204.89			2386.7					7.87		675			6.30	5.72	0.36	59.94	5.75	358.54	59.79		0.91	57	9.44										1112	54.1	9.01
Calcidiscus leptoporus	haploid C	pCO2=760	33.6	19	160	12:12			5633000		0.627		2234.80			2411.0					7.86		708			6.63	5.41	0.36	62.75	5.40	355.55	62.69		0.89	59	10.32										833	43.9	7.80
Syracosphaera pulchra	diploid A	pCO2=400	33.6	19	160	12:12				6554000	0.626	1980.07	1924.59	1956.65	2222.3	2202.3	2215.9	13.8	11.4	8.03	8.10	416	339	178.0	183.0	19.13	5.82	1.11	169.62	4.70	897.35	136.92	32.71	0.72	138	21.07	15	8.09	11.41	340.81	339.65	1761.78	183.47	2.86	4.43	1031	16.2	2.46
Syracosphaera pulchra	diploid B	pCO2=400	33.6	19	160	12:12				5202000	0.597		1941.15			2213.0					8.08		354			21.67	4.94	1.07	205.09	3.92	841.07	161.68	43.41	0.58	124	23.91										838	12.0	2.31
Syracosphaera pulchra	diploid C	pCO2=400	33.6	19	160	12:12				6801000	0.639		1933.95			2213.3					8.10		338			20.19	4.99	1.00	147.00	4.52	905.67	133.17	13.83	0.69	137	20.21										992	35.0	5.15
Syracosphaera pulchra	haploid A	pCO2=400	33.6	19	160	12:12	4.3			3969000	0.531	1980.07	1963.89	1968.25	2222.3	2244.8	2229.6	13.8	11.3	8.03	8.10	416	344	178.0	188.0	22.56	3.57	0.80	202.09	3.55	801.96	202.05		0.53	120	30.27	15	8.10	11.42	341.26	340.10	1771.56	185.27	2.89	4.47	559	18.3	4.60
Syracosphaera pulchra	haploid B	pCO2=400	33.6	19	160	12:12				3964000	0.527		1956.92			2236.7					8.10		342			17.76	4.58	0.80	202.86	4.53	797.92	201.29	0.99	0.68	120	30.35										628	16.8	4.24
Syracosphaera pulchra	haploid C	pCO2=400	33.6	19	160	12:12				3870000	0.531		1948.50			2229.3					8.10		338			22.62	3.46	0.78	202.20	3.46	782.03	202.07	0.12	0.51	116	30.03										443	22.0	5.68
Syracosphaera pulchra	diploid A	pCO2=760	33.6	19	160	12:12	2.9			7377000	0.647	2087.52	2046.41	2072.21	2235.0	2222.7	2232.6	24.1	20.1	7.82	7.89	730	601	119.0	125.0	19.06	4.99	0.93	125.82	4.15	778.70	105.56	20.26	0.77	145	19.63	15	7.88	20.40	609.66	607.59	1928.87	122.94	1.92	2.97	1805	20.9	2.83
Syracosphaera pulchra	diploid B	pCO2=760	33.6	19	160	12:12				7717000	0.658		2064.51			2234.7					7.87		632			22.25	4.27	0.95	122.47	3.38	750.52	97.26	25.21	0.65	143	18.56										1979	15.8	2.04
Syracosphaera pulchra	diploid C	pCO2=760	33.6	19	160	12:12				7870000	0.660		2059.76			2233.0					7.88		622			22.78	3.94	0.90	114.09	3.22	732.89	93.12	20.97	0.60	136	17.31										1045	13.9	1.76
Syracosphaera pulchra	haploid A	pCO2=760	33.6	19	160	12:12	2.9			10699000	0.631	2087.52	2012.64	2058.52	2235.0	2252.3	2247.3	24.1	15.4	7.82	8.02	730	428	119.0	158.0	20.35	5.22	1.03	95.95	5.16	1015.05	94.87	0.72	0.90	177	16.57	15	7.95	17.34	518.24	516.49	1900.74	140.44	2.19	3.39	475	11.6	1.08
Syracosphaera pulchra	haploid B	pCO2=760	33.6	19	160	12:12				9213000	0.580		2039.76			2260.3					7.98		482			18.98	4.79	0.91	98.70	4.77	905.29	98.26	0.46	0.85	161	17.51										461	4.6	0.49
Syracosphaera pulchra	haploid C	pCO2=760	33.6	19	160	12:12				10409000	0.614		2036.14			2266.0					8.00		460			19.49	5.11	0.98	94.45	5.10	980.03	94.15	0.61	0.89	172	16.50										502	9.7	0.93
Syracosphaera pulchra	diploid A	pCO2=400	33.6	22	160	12:12	4.8			4258000	0.623	1933.78	1909.76	1923.62	2205.0	2197.7	2200.6	11.8	11.2	8.06	8.07	379	356	198.0	189.0	17.19	4.26	0.73	172.05	3.60	619.07	145.39	26.66	0.57	98	22.95	15	8.08	10.69	346.81	345.68	1717.54	195.39	3.08	4.73
Syracosphaera pulchra	diploid B	pCO2=400	33.6	22	160	12:12				5393000	0.663		1916.18			2200.0					8.08		354			24.84	3.32	0.82	152.66	2.78	691.32	128.19	24.47	0.43	107	19.90
Syracosphaera pulchra	diploid C	pCO2=400	33.6	22	160	12:12				3727000	0.601		1914.45			2191.0					8.06		368			21.61	3.17	0.68	182.23	2.79	602.59	161.68	20.55	0.45	95	25.61
Syracosphaera pulchra	haploid A	pCO2=400	33.6	22	160	12:12	4.8			14442000	0.623	1933.78	1776.18	1861.07	2205.0	2197.0	2201.8	11.8	6.2	8.05	8.29	379	188	198.0	270.0	30.96	5.70	1.71	118.07	5.68	1702.14	117.86	0.21	0.74	221	15.29	15	8.19	7.83	253.98	253.15	1616.81	236.43	3.73	5.73
Syracosphaera pulchra	haploid B	pCO2=400	33.6	22	160	12:12				16248000	0.631		1788.80			2198.8					8.26		200			36.93	4.66	1.71	127.58	4.50	1653.10	126.68	0.19	0.63	231	14.22
Syracosphaera pulchra	haploid C	pCO2=400	33.6	22	160	12:12				10598000	0.596		1800.08			2200.0					8.25		212			36.76	3.91	1.44	135.60	3.90	1435.52	135.45	0.15	0.54	200	18.91
Syracosphaera pulchra	diploid A	pCO2=750	33.6	22	160	12:12	3.2			5549000	0.594	2077.92	2063.29	2071.99	2222.0	2237.3	2233.6	22.5	20.3	7.80	7.85	751	677	133.0	130.0	24.46	4.00	0.98	176.19	3.34	817.92	147.40	28.79	0.48	118	21.33	15	7.84	21.03	682.17	679.94	1926.05	124.92	1.97	3.03
Syracosphaera pulchra	diploid B	pCO2=750	33.6	22	160	12:12				6386000	0.595		2051.72			2232.3					7.86		658			19.21	4.82	0.91	142.87	4.33	821.69	128.67	14.20	0.60	114	17.84
Syracosphaera pulchra	diploid C	pCO2=750	33.6	22	160	12:12				5974000	0.598		2083.18			2266.0					7.86		661			19.39	4.87	0.94	157.59	4.26	824.27	137.98	19.62	0.60	116	19.41
Syracosphaera pulchra	haploid A	pCO2=750	33.6	22	160	12:12	3.1			8957000	0.586	2063.62	1998.05	2035.20	2234.0	2244.6	2241.2	22.7	14.1	7.81	8.00	745	449	129.0	169.0	25.34	4.15	1.05	116.51	4.09	1036.41	115.71		0.58	147	16.37	15	7.94	16.23	526.45	524.73	1866.90	152.08	2.40	3.68
Syracosphaera pulchra	haploid B	pCO2=750	33.6	22	160	12:12				10319000	0.597		2006.36			2248.5					7.98		462			20.64	5.25	1.09	109.25	5.06	1041.94	108.60		0.74	152	14.74
Syracosphaera pulchra	haploid C	pCO2=750	33.6	22	160	12:12				9491000	0.590		2015.91			2252.3					7.99		471			24.11	4.00	0.96	101.54	4.00	963.25	101.49		0.59	142	14.96