Citation:

j.jembe.2011.06.008

Name: Seawater carbonate chemistry and biological processes of Emiliania huxleyi (strains RCC1256 and NZEH) during experiments, 2011
Published: 2011-08-12
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; South Pacific

Always quote citation above when using data! You can download the citation in several formats below.

RIS Citation BibTeX Citation Text Citation Facebook Twitter

Abstract:

With respect to their sensitivity to ocean acidification, calcifiers such as the coccolithophore Emiliania huxleyi have received special attention, as the process of calcification seems to be particularly sensitive to changes in the marine carbonate system. For E. huxleyi, apparently conflicting results regarding its sensitivity to ocean acidification have been published (Iglesias-Rodriguez et al., 2008a; Riebesell et al., 2000). As possible causes for discrepancies, intra-specific variability and different effects of CO2 manipulation methods, i.e. the manipulation of total alkalinity (TA) or total dissolved inorganic carbon (DIC), have been discussed. While Langer et al. (2009) demonstrate a high degree of intra-specific variability between strains of E. huxleyi, the question whether different CO2 manipulation methods influence the cellular responses has not been resolved yet. In this study, closed TA as well as open and closed DIC manipulation methods were compared with respect to E. huxleyi's CO2-dependence in growth rate, POC- and PIC-production. The differences in the carbonate chemistry between TA and DIC manipulations were shown not to cause any differences in response patterns, while the latter differed between open and closed DIC manipulation. The two strains investigated showed different sensitivities to acidification of seawater, RCC1256 being more negatively affected in growth rates and PIC production than NZEH.

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; South Pacific

Project(s):

Biological Impacts of Ocean Acidification (BIOACID)

European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)

European Project on Ocean Acidification (EPOCA)

Mediterranean Sea Acidification in a Changing Climate (MedSeA)

Ocean Acidification International Coordination Centre (OA-ICC)

Funding:

Seventh Framework Programme (FP7), grant/award no. 211384 : European Project on Ocean Acidification

Seventh Framework Programme (FP7), grant/award no. 265103 : Mediterranean Sea Acidification in a Changing Climate

Sixth Framework Programme (FP6), grant/award no. 511106 : European network of excellence for Ocean Ecosystems Analysis

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Hoppe, Clara Jule Marie
2	Experimental treatment	Exp treat		Hoppe, Clara Jule Marie
3	Comment	Comment		Hoppe, Clara Jule Marie
4	Sample ID	Sample ID		Hoppe, Clara Jule Marie
5	Salinity	Sal		Hoppe, Clara Jule Marie
6	Temperature, water	Temp	°C	Hoppe, Clara Jule Marie
7	Alkalinity, total	AT	µmol/kg	Hoppe, Clara Jule Marie	Alkalinity, Gran titration (Gran, 1950)
8	Carbon, inorganic, dissolved	DIC	µmol/kg	Hoppe, Clara Jule Marie	Seal QuAAtro SFA Analyzer, Seal Analytical, 800 TM
9	pH	pH		Hoppe, Clara Jule Marie	Calculated using CO2SYS	NBS scale
10	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Hoppe, Clara Jule Marie	Calculated using CO2SYS
11	Bicarbonate ion	[HCO3]-	µmol/kg	Hoppe, Clara Jule Marie	Calculated using CO2SYS
12	Carbonate ion	[CO3]2-	µmol/kg	Hoppe, Clara Jule Marie	Calculated using CO2SYS
13	Carbon dioxide	CO2	µmol/kg	Hoppe, Clara Jule Marie	Calculated using CO2SYS
14	Calcite saturation state	Omega Cal		Hoppe, Clara Jule Marie	Calculated using CO2SYS
15	Growth rate	µ	#/day	Hoppe, Clara Jule Marie	Calculated
16	Carbon, organic, particulate, per cell	POC/cell	pg/#	Hoppe, Clara Jule Marie	Mass spectrometer ANCA-SL 20-20 Europa Scientific
17	Particulate organic carbon production per cell	POC prod/cell	pg/#/day	Hoppe, Clara Jule Marie	Calculated
18	Carbon, inorganic, particulate, per cell	PIC/cell	pg/#	Hoppe, Clara Jule Marie	Calculated
19	Particulate inorganic carbon production per cell	PIC prod/cell	pg/#/day	Hoppe, Clara Jule Marie	Calculated
20	Particulate inorganic carbon/particulate organic carbon ratio	PIC/POC		Hoppe, Clara Jule Marie	Calculated
21	Carbonate system computation flag	CSC flag		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
22	pH	pH		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)	Total scale
23	Carbon dioxide	CO2	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
24	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
25	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
26	Bicarbonate ion	[HCO3]-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
27	Carbonate ion	[CO3]2-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
28	Aragonite saturation state	Omega Arg		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
29	Calcite saturation state	Omega Cal		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1638 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 Exp treat	3 Comment	4 Sample ID	5 Sal	6 Temp [°C]	7 AT [µmol/kg] (Alkalinity, Gran titration (G...)	8 DIC [µmol/kg] (Seal QuAAtro SFA Analyzer, Se...)	9 pH (NBS scale, Calculated using C...)	10 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	11 [HCO3]- [µmol/kg] (Calculated using CO2SYS)	12 [CO3]2- [µmol/kg] (Calculated using CO2SYS)	13 CO2 [µmol/kg] (Calculated using CO2SYS)	14 Omega Cal (Calculated using CO2SYS)	15 µ [#/day] (Calculated)	16 POC/cell [pg/#] (Mass spectrometer ANCA-SL 20-...)	17 POC prod/cell [pg/#/day] (Calculated)	18 PIC/cell [pg/#] (Calculated)	19 PIC prod/cell [pg/#/day] (Calculated)	20 PIC/POC (Calculated)	21 CSC flag (Calculated using seacarb afte...)	22 pH (Total scale, Calculated using...)	23 CO2 [µmol/kg] (Calculated using seacarb afte...)	24 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	25 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	26 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	27 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	28 Omega Arg (Calculated using seacarb afte...)	29 Omega Cal (Calculated using seacarb afte...)
Emiliania huxleyi (RCC1256)	pCO2=180	TA manipulation closed	1	32	15	2603	2125	8.29	310	1903.22	210.15	11.76	5.11	1.18						15	8.45	5.61	147.76	147.24	1772.66	346.73	5.38	8.42
Emiliania huxleyi (RCC1256)	pCO2=180	TA manipulation closed	2	32	15	2453	2106	8.32	285	1873.59	221.67	10.80	5.39	1.23	10.22	12.52	9.42	11.54	0.92	15	8.29	8.45	222.54	221.75	1847.49	250.06	3.88	6.08
Emiliania huxleyi (RCC1256)	pCO2=180	TA manipulation closed	3	32	15	2469	2111	8.34	271	1869.09	231.56	10.29	5.63	1.16	10.49	12.21	10.96	12.75	1.04	15	8.31	8.16	214.93	214.17	1844.70	258.14	4.00	6.27
Emiliania huxleyi (RCC1256)	pCO2=380	TA manipulation closed	1	32	15	2320	2108	7.91	776	1986.64	91.45	29.44	2.22	1.14						15	8.07	14.82	390.37	388.98	1936.55	156.63	2.43	3.81
Emiliania huxleyi (RCC1256)	pCO2=380	TA manipulation closed	2	32	15	2285	2091	8.00	621	1956.54	110.80	23.57	2.69	1.18	12.60	14.88	8.71	10.29	0.69	15	8.03	15.99	421.22	419.72	1930.72	144.29	2.24	3.51
Emiliania huxleyi (RCC1256)	pCO2=380	TA manipulation closed	3	32	15	2292	2093	8.00	622	1958.83	110.93	23.59	2.70	1.20	12.18	14.63	9.01	10.82	0.74	15	8.04	15.61	411.32	409.85	1929.77	147.62	2.29	3.59
Emiliania huxleyi (RCC1256)	pCO2=750	TA manipulation closed	1	32	15	2189	2069	7.82	942	1960.32	73.34	35.74	1.78	1.08	11.55	12.51				15	7.86	24.06	633.77	631.51	1947.38	97.56	1.51	2.37
Emiliania huxleyi (RCC1256)	pCO2=750	TA manipulation closed	2	32	15	2176	2065	7.82	940	1956.15	73.19	35.66	1.78	1.07	11.84	12.65	6.72	7.19	0.57	15	7.84	25.45	670.43	668.04	1947.33	92.22	1.43	2.24
Emiliania huxleyi (RCC1256)	pCO2=750	TA manipulation closed	3	32	15	2154	2055	7.81	957	1947.68	71.21	36.34	1.73	1.15	10.49	12.09	6.72			15	7.80	27.42	722.43	719.86	1942.43	85.15	1.32	2.07
Emiliania huxleyi (RCC1256)	pCO2=1000	TA manipulation closed	1	32	15	2162	2087	7.73	1172	1982.72	60.30	44.47	1.47	1.07	13.54	14.47	6.69	7.15	0.49	15	7.73	33.26	876.25	873.13	1980.74	73.00	1.13	1.77
Emiliania huxleyi (RCC1256)	pCO2=1000	TA manipulation closed	2	32	15	2167	2084	7.71	1225	1980.06	57.51	46.50	1.40	1.07	12.00	12.80	7.46	7.96	0.62	15	7.75	31.31	824.87	821.93	1975.55	77.14	1.20	1.87
Emiliania huxleyi (RCC1256)	pCO2=1000	TA manipulation closed	3	32	15	2152	2075	7.71	1220	1971.70	57.26	46.31	1.39	1.04	13.67	14.19	7.38	7.66	0.54	15	7.73	32.53	856.95	853.89	1968.73	73.74	1.14	1.79
Emiliania huxleyi (RCC1256)	pCO2=180	DIC manipulation closed	1	32	15	2357	1952	8.44	194	1682.35	262.40	7.36	6.38	1.16	14.48	16.85	9.92	11.54	0.69	15	8.40	5.97	157.36	156.80	1660.39	285.64	4.43	6.94
Emiliania huxleyi (RCC1256)	pCO2=180	DIC manipulation closed	2	32	15	2343	1932	8.44	192	1665.08	259.70	7.28	6.31	1.16	12.58	14.56	10.35	11.98	0.82	15	8.41	5.74	151.16	150.62	1637.16	289.10	4.48	7.02
Emiliania huxleyi (RCC1256)	pCO2=180	DIC manipulation closed	3	32	15	2348	1935	8.45	187	1662.12	265.28	7.10	6.45	1.18	12.59	14.81	10.50	12.34	0.83	15	8.41	5.72	150.61	150.07	1638.61	290.68	4.51	7.06
Emiliania huxleyi (RCC1256)	pCO2=380	DIC manipulation closed	1	32	15	2352	2063	8.21	367	1876.75	172.36	13.94	4.19	1.14	11.95	13.65	9.57	10.92	0.80	15	8.21	10.15	267.39	266.44	1845.24	207.61	3.22	5.04
Emiliania huxleyi (RCC1256)	pCO2=380	DIC manipulation closed	2	32	15	2336	2057	8.20	375	1874.24	168.22	14.24	4.09	1.19	12.30	14.66	9.65	11.50	0.78	15	8.20	10.52	277.14	276.15	1846.00	200.48	3.11	4.87
Emiliania huxleyi (RCC1256)	pCO2=380	DIC manipulation closed	3	32	15	2343	2054	8.18	394	1877.86	160.96	14.94	3.91	1.19	12.23	14.51	10.64	12.62	0.87	15	8.21	10.07	265.30	264.35	1836.62	207.31	3.22	5.04
Emiliania huxleyi (RCC1256)	pCO2=750	DIC manipulation closed	1	32	15	2322	2131	7.98	664	1997.75	108.04	25.20	2.63	1.15	11.26	12.90	8.15	9.33	0.72	15	8.02	16.75	441.34	439.77	1970.77	143.48	2.23	3.49
Emiliania huxleyi (RCC1256)	pCO2=750	DIC manipulation closed	2	32	15	2309	2119	7.99	645	1984.94	109.85	24.47	2.67	1.22	11.49	13.99	8.71	10.59	0.76	15	8.02	16.68	439.51	437.94	1959.83	142.49	2.21	3.46
Emiliania huxleyi (RCC1256)	pCO2=750	DIC manipulation closed	3	32	15	2317	2117	7.98	660	1984.92	107.35	25.04	2.61	1.18	13.03	15.34	8.60	10.13	0.66	15	8.04	15.83	417.11	415.62	1952.20	148.97	2.31	3.62
Emiliania huxleyi (RCC1256)	pCO2=1000	DIC manipulation closed	1	32	15	2337	2164	7.89	835	2042.27	89.77	31.69	2.18	1.04	11.15	11.65	7.58	7.92	0.68	15	7.98	18.88	497.31	495.53	2012.36	132.77	2.06	3.23
Emiliania huxleyi (RCC1256)	pCO2=1000	DIC manipulation closed	2	32	15	2357	2157	7.87	873	2038.67	85.58	33.13	2.08	1.12	12.32	13.85	8.11	9.12	0.66	15	8.04	16.33	430.32	428.78	1990.54	150.13	2.33	3.65
Emiliania huxleyi (RCC1256)	pCO2=1000	DIC manipulation closed	3	32	15	2333	2148	7.89	829	2027.51	89.13	31.46	2.17	1.14	13.08	14.88	9.49	10.79	0.73	15	8.01	17.51	461.19	459.55	1990.43	140.06	2.17	3.40
Emiliania huxleyi (NZEH)	pCO2=180	TA manipulation closed	1	32	15	2591	2096	8.40	231	1827.13	259.90	8.76	6.32	1.15	11.50	13.21	8.96	10.30	0.78	15	8.48	5.19	136.66	136.17	1732.65	358.17	5.55	8.70
Emiliania huxleyi (NZEH)	pCO2=180	TA manipulation closed	2	32	15	2553	2087	8.39	236	1824.86	253.67	8.95	6.17	1.29	8.47	10.90	12.90	16.61	1.52	15	8.44	5.61	147.74	147.21	1745.25	336.15	5.21	8.17
Emiliania huxleyi (NZEH)	pCO2=180	TA manipulation closed	3	32	15	2576	2085	8.40	230	1817.76	258.57	8.72	6.28	1.19	9.72	11.55	11.06	13.14	1.14	15	8.47	5.19	136.81	136.32	1725.13	354.68	5.50	8.62
Emiliania huxleyi (NZEH)	pCO2=380	TA manipulation closed	1	32	15	2382	2082	8.18	399	1903.34	163.14	15.15	3.97	1.19	9.63	11.49	10.04	11.98	1.04	15	8.23	9.87	260.15	259.22	1856.19	215.94	3.35	5.25
Emiliania huxleyi (NZEH)	pCO2=380	TA manipulation closed	2	32	15	2363	2071	8.22	360	1880.93	176.77	13.65	4.30	1.19	10.42	12.46	10.76	12.86	1.03	15	8.21	10.10	266.02	265.07	1850.93	209.98	3.26	5.10
Emiliania huxleyi (NZEH)	pCO2=380	TA manipulation closed	3	32	15	2352	2056	8.23	348	1863.31	179.19	13.22	4.36	1.26	11.00	13.85	11.55	14.55	1.05	15	8.22	9.81	258.37	257.45	1833.95	212.25	3.29	5.16
Emiliania huxleyi (NZEH)	pCO2=750	TA manipulation closed	1	32	15	2258	2038	7.92	733	1919.89	90.43	27.80	2.20	1.19	10.72	12.73	9.60	11.39	0.90	15	8.09	13.46	354.69	353.43	1864.70	159.84	2.48	3.88
Emiliania huxleyi (NZEH)	pCO2=750	TA manipulation closed	2	32	15	2257	2047	7.97	653	1921.09	101.53	24.80	2.47	1.28	10.79	13.81	9.47	12.13	0.88	15	8.07	14.24	375.15	373.82	1879.27	153.49	2.38	3.73
Emiliania huxleyi (NZEH)	pCO2=750	TA manipulation closed	3	32	15	2257	2053	7.97	655	1926.59	101.82	24.87	2.47	1.18	12.27	14.47	10.86	12.81	0.88	15	8.06	14.74	388.40	387.02	1888.54	149.72	2.32	3.64
Emiliania huxleyi (NZEH)	pCO2=1000	TA manipulation closed	1	32	15	2235	2083	7.69	1283	1979.50	54.90	48.68	1.33	1.21	10.52	12.71	8.64	10.44	0.82	15	7.94	19.98	526.27	524.40	1945.73	117.29	1.82	2.85
Emiliania huxleyi (NZEH)	pCO2=1000	TA manipulation closed	2	32	15	2236	2087	7.80	995	1978.07	70.68	37.76	1.72	1.17	11.11	13.05	9.37	11.00	0.84	15	7.93	20.39	537.18	535.26	1951.07	115.54	1.79	2.81
Emiliania huxleyi (NZEH)	pCO2=1000	TA manipulation closed	3	32	15	2237	2073	7.70	1247	1970.05	55.91	47.35	1.36	1.15	11.30	13.01	10.00	11.52	0.89	15	7.97	18.51	487.70	485.97	1929.97	124.52	1.93	3.03
Emiliania huxleyi (NZEH)	pCO2=180	DIC manipulation closed	1	32	15	2332	1923	8.15	397	1766.42	141.30	15.06	3.43	1.20	11.72	14.02	11.16	13.35	0.95	15	8.41	5.72	150.80	150.26	1630.01	287.26	4.46	6.98
Emiliania huxleyi (NZEH)	pCO2=180	DIC manipulation closed	2	32	15	2304	1913	8.14	405	1759.61	137.55	15.35	3.34	1.22	11.18	13.66	16.68	20.38	1.49	15	8.38	6.03	158.75	158.18	1633.07	273.90	4.25	6.65
Emiliania huxleyi (NZEH)	pCO2=180	DIC manipulation closed	3	32	15	2302	1899	8.13	412	1750.01	133.68	15.63	3.25	1.24	11.63	14.42	12.49	15.49	1.07	15	8.40	5.70	150.18	149.65	1611.41	281.89	4.37	6.85
Emiliania huxleyi (NZEH)	pCO2=380	DIC manipulation closed	1	32	15	2271	2020	7.94	692	1900.05	93.71	26.28	2.28	1.24	12.48	15.44	10.73	13.27	0.86	15	8.15	11.49	302.68	301.60	1828.43	180.08	2.79	4.38
Emiliania huxleyi (NZEH)	pCO2=380	DIC manipulation closed	2	32	15	2266	1998	7.97	637	1874.31	99.06	24.19	2.41	1.27	12.08	15.38	12.04	15.33	1.00	15	8.19	10.46	275.64	274.65	1796.61	190.93	2.96	4.64
Emiliania huxleyi (NZEH)	pCO2=380	DIC manipulation closed	3	32	15	2292	2058	7.95	689	1933.93	97.61	26.14	2.37	1.23	12.33	15.18	10.25	12.62	0.83	15	8.12	12.82	337.68	336.47	1875.37	169.81	2.63	4.13
Emiliania huxleyi (NZEH)	pCO2=750	DIC manipulation closed	1	32	15	2292	2116	7.85	897	2002.00	80.26	34.06	1.95	1.20	11.79	14.13	10.58	12.68	0.90	15	7.99	17.91	471.96	470.27	1964.73	133.35	2.07	3.24
Emiliania huxleyi (NZEH)	pCO2=750	DIC manipulation closed	2	32	15	2294	2121	7.80	1011	2010.51	71.84	38.38	1.75	1.15	12.19	14.04	10.46	12.05	0.86	15	7.98	18.28	481.48	479.76	1971.15	131.57	2.04	3.20
Emiliania huxleyi (NZEH)	pCO2=750	DIC manipulation closed	3	32	15	2297	2118	7.82	964	2006.16	75.06	36.57	1.82	1.22	12.67	15.47	11.07	13.51	0.87	15	8.00	17.66	465.17	463.51	1965.01	135.34	2.10	3.29
Emiliania huxleyi (NZEH)	pCO2=1000	DIC manipulation closed	1	32	15	2308	2160	7.79	1054	2048.75	71.54	40.02	1.74	1.09	12.45	13.55	10.19	11.08	0.82	15	7.92	21.64	569.98	567.95	2021.47	116.89	1.81	2.84
Emiliania huxleyi (NZEH)	pCO2=1000	DIC manipulation closed	2	32	15	2313	2195	7.80	1047	2080.94	74.35	39.73	1.81	1.20	13.66	16.40	10.79	12.96	0.79	15	7.84	26.57	700.12	697.62	2068.76	99.67	1.55	2.42
Emiliania huxleyi (NZEH)	pCO2=1000	DIC manipulation closed	3	32	15	2313	2197	7.78	1098	2084.65	71.13	41.67	1.73	1.18	13.94	16.43	9.87	11.64	0.71	15	7.84	26.95	709.98	707.44	2071.51	98.54	1.53	2.39
Emiliania huxleyi (NZEH)	pCO2=180	DIC manipulation open	1	32	15	2345	1908	8.51	158	1607.55	294.58	5.98	7.16	1.08	8.56	9.28	8.42	9.14	0.98	15	8.44	5.14	135.35	134.87	1596.02	306.84	4.76	7.45
Emiliania huxleyi (NZEH)	pCO2=180	DIC manipulation open	2	32	15	2360	1927	8.51	159	1623.61	297.52	6.04	7.23	0.85	9.86	8.41	10.83	9.23	1.10	15	8.44	5.31	139.84	139.34	1616.89	304.81	4.73	7.41
Emiliania huxleyi (NZEH)	pCO2=180	DIC manipulation open	3	32	15	2347	1905	8.52	153	1599.44	299.92	5.82	7.29	1.03	7.29	7.54	7.50	7.75	1.03	15	8.45	5.04	132.76	132.28	1589.62	310.34	4.81	7.54
Emiliania huxleyi (NZEH)	pCO2=380	DIC manipulation open	1	32	15	2328	2053	8.25	331	1853.57	186.66	12.56	4.54	1.09	8.66	9.44	10.05	10.96	1.16	15	8.19	10.66	280.82	279.82	1844.76	197.59	3.06	4.80
Emiliania huxleyi (NZEH)	pCO2=380	DIC manipulation open	2	32	15	2315	2051	8.26	322	1848.15	190.45	12.23	4.63	1.06	8.74	9.27	9.38	9.96	1.07	15	8.17	11.15	293.72	292.68	1849.89	189.96	2.95	4.62
Emiliania huxleyi (NZEH)	pCO2=380	DIC manipulation open	3	32	15	2322	2050	8.22	356	1861.41	174.94	13.51	4.25	1.09	9.49	10.33	9.04	9.85	0.95	15	8.19	10.77	283.64	282.63	1843.82	195.42	3.03	4.75
Emiliania huxleyi (NZEH)	pCO2=1000	DIC manipulation open	1	32	15	2358	2210	7.87	894	2088.09	87.66	33.93	2.13	1.06	10.18	10.83	6.68	7.10	0.66	15	7.92	22.42	590.57	588.46	2069.36	118.22	1.83	2.87
Emiliania huxleyi (NZEH)	pCO2=1000	DIC manipulation open	2	32	15	2362	2210	7.84	959	2091.33	81.93	36.41	1.99	1.13	9.62	10.85	7.60	8.57	0.79	15	7.93	21.91	577.26	575.21	2067.37	120.72	1.87	2.93
Emiliania huxleyi (NZEH)	pCO2=1000	DIC manipulation open	3	32	15	2363	2213	7.88	874	2089.66	89.77	33.18	2.18	1.07	9.40	10.01	7.83	8.34	0.83	15	7.92	22.21	585.09	583.00	2071.24	119.55	1.85	2.90