Citation:

S0025315410000457

Name: Seawater carbonate chemistry and biological processes during experiments with Patella vulgata, 2010
Published: 2010
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Acid-base regulation; Animalia; Behaviour; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Growth/Morphology; Laboratory experiment; Mollusca; North Atlantic; Patella vulgata; Respiration; Single species; Temperate

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Abstract:

The effect of short-term (5 days) exposure to CO2-acidified seawater (year 2100 predicted values, ocean pH = 7.6) on key aspects of the function of the intertidal common limpet Patella vulgata (Gastropoda: Patellidae) was investigated. Changes in extracellular acid-base balance were almost completely compensated by an increase in bicarbonate ions. A concomitant increase in haemolymph Ca2+ and visible shell dissolution implicated passive shell dissolution as the bicarbonate source. Analysis of the radula using SEM revealed that individuals from the hypercapnic treatment showed an increase in the number of damaged teeth and the extent to which such teeth were damaged compared with controls. As radula teeth are composed mainly of chitin, acid dissolution seems unlikely, and so the proximate cause of damage is unknown. There was no hypercapnia-related change in metabolism (O2 uptake) or feeding rate, also discounting the possibility that teeth damage was a result of a CO2-related increase in grazing. We conclude that although the limpet appears to have the physiological capacity to maintain its extracellular acid-base balance, metabolism and feeding rate over a 5 days exposure to acidified seawater, radular damage somehow incurred during this time could still compromise feeding in the longer term, in turn decreasing the top-down ecosystem control that P. vulgata exerts over rocky shore environments.

Keyword(s):

Acid-base regulation; Animalia; Behaviour; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Growth/Morphology; Laboratory experiment; Mollusca; North Atlantic; Patella vulgata; Respiration; Single species; Temperate

Project(s):

European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)

European Project on Ocean Acidification (EPOCA)

Ocean Acidification International Coordination Centre (OA-ICC)

Funding:

Seventh Framework Programme (FP7), grant/award no. 211384 : European Project on Ocean Acidification

Sixth Framework Programme (FP6), grant/award no. 511106 : European network of excellence for Ocean Ecosystems Analysis

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Experimental treatment	Exp treat		Calosi, Piero
2	Identification	ID		Calosi, Piero
3	Carbonate system computation flag	CSC flag		Calosi, Piero
4	Salinity	Sal		Calosi, Piero
5	Temperature, water	Temp	°C	Calosi, Piero
6	pH	pH		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)	Total scale
7	Carbon dioxide	CO2	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
8	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
9	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
10	Bicarbonate ion	[HCO3]-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
11	Carbonate ion	[CO3]2-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
12	Carbon, inorganic, dissolved	DIC	µmol/kg	Calosi, Piero	CO2-Analyser Corning
13	Alkalinity, total	AT	µmol/kg	Calosi, Piero	Calculated using CO2SYS
14	Aragonite saturation state	Omega Arg		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
15	Calcite saturation state	Omega Cal		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
16	Patella vulgata, haemolymph, pH	P. vulgata pH (ha)		Calosi, Piero	pH meter (Mettler Toledo InLab 413 SG)
17	Patella vulgata, haemolymph, carbon dioxide	P. vulgata CO2 (ha)	mmol/l	Calosi, Piero	CO2-Analyser Corning
18	Patella vulgata, haemolymph, bicarbonate ion	P. vulgata [HCO3]- (ha)	mmol/l	Calosi, Piero	Henderson-Hasselback equasion (Spicer et al., 1988)
19	Respiration, oxygen	Resp O2	µl/mg	Calosi, Piero	Closed respirometer technique (Spicer & Eriksson, 2003)
20	Patella vulgata, weight	P. vulgata W	g	Calosi, Piero
21	Patella vulgata, tooth, area, worn away	P. vulgata tooth worn away	nm²	Calosi, Piero
22	Feeding rate, relative	Feed rate		Calosi, Piero		Day 1
23	Feeding rate, relative	Feed rate		Calosi, Piero		Day 2
24	Feeding rate, relative	Feed rate		Calosi, Piero		Day 3
25	Feeding rate, relative	Feed rate		Calosi, Piero		Day 4
26	Feeding rate, relative	Feed rate		Calosi, Piero		Day 5

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

480 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Exp treat	2 ID	3 CSC flag	4 Sal	5 Temp [°C]	6 pH (Total scale, Calculated using...)	7 CO2 [µmol/kg] (Calculated using seacarb afte...)	8 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	9 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	10 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	11 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	12 DIC [µmol/kg] (CO2-Analyser Corning)	13 AT [µmol/kg] (Calculated using CO2SYS)	14 Omega Arg (Calculated using seacarb afte...)	15 Omega Cal (Calculated using seacarb afte...)	16 P. vulgata pH (ha) (pH meter (Mettler Toledo InLa...)	17 P. vulgata CO2 (ha) [mmol/l] (CO2-Analyser Corning)	18 P. vulgata [HCO3]- (ha) [mmol/l] (Henderson-Hasselback equasion...)	19 Resp O2 [µl/mg] (Closed respirometer technique...)	20 P. vulgata W [g]	21 P. vulgata tooth worn away [nm²]	22 Feed rate (Day 1)	23 Feed rate (Day 2)	24 Feed rate (Day 3)	25 Feed rate (Day 4)	26 Feed rate (Day 5)
Control	1	27	35	14	8.13	16.05	414.94	413.44	2400.23	228.36	2644.6	2942.7	3.48	5.44	5.92	0.05	0.022	43.723	31.5	631	19.00	2.67	0.95	0.00	14.05
Control	2	27	35	14	8.13	16.05	414.94	413.44	2400.23	228.36	2644.6	2942.7	3.48	5.44	7.84	4.90	4.824	34.463	31.3	285	0.00	0.00	0.00	4.62	27.92
Control	3	27	35	14	8.13	16.05	414.94	413.44	2400.23	228.36	2644.6	2942.7	3.48	5.44	6.83	3.60	3.098	47.660	25.3	129	4.97	6.08	17.47	11.77	19.42
Control	4	27	35	14	8.13	16.05	414.94	413.44	2400.23	228.36	2644.6	2942.7	3.48	5.44	7.80	3.10	3.047	31.864	23.3	334	0.00	0.00	0.00	6.16	14.46
Control	5	27	35	14	8.13	16.05	414.94	413.44	2400.23	228.36	2644.6	2942.7	3.48	5.44	5.16	0.08	0.009	70.368	24.0	435	0.00	36.16	0.00	18.18	11.41
Control	6	27	35	14	8.13	16.05	414.94	413.44	2400.23	228.36	2644.6	2942.7	3.48	5.44	7.36	3.20	3.054	28.361	23.3	50
Control	7	27	35	14	8.13	16.05	414.94	413.44	2400.23	228.36	2644.6	2942.7	3.48	5.44	7.67	6.20	6.058			290
Control	8	27	35	14	8.13	16.05	414.94	413.44	2400.23	228.36	2644.6	2942.7	3.48	5.44	6.07	1.50	0.776			567
Control	9	27	35	14	8.13	16.05	414.94	413.44	2400.23	228.36	2644.6	2942.7	3.48	5.44	5.36	0.04	0.007			1515
Control	10	27	35	14	8.13	16.05	414.94	413.44	2400.23	228.36	2644.6	2942.7	3.48	5.44	6.17	1.20	0.689
Acidified	1	27	35	14	7.42	108.01	2792.20	2782.10	3150.37	58.44	3316.8	3287.0	0.89	1.39	7.63	6.00	5.850	25.090	23.3	921	3.22	18.07	0.00	0.00	5.17
Acidified	2	27	35	14	7.42	108.01	2792.20	2782.10	3150.37	58.44	3316.8	3287.0	0.89	1.39	6.41	6.50	4.556	19.292	25.5	1040	4.66	35.54	29.66	0.00	0.00
Acidified	3	27	35	14	7.42	108.01	2792.20	2782.10	3150.37	58.44	3316.8	3287.0	0.89	1.39	7.49	7.80	7.533	35.142	23.5	2910	3.20	0.00	23.52	24.84	3.28
Acidified	4	27	35	14	7.42	108.01	2792.20	2782.10	3150.37	58.44	3316.8	3287.0	0.89	1.39	7.37	5.40	5.159	10.753	37.1	1812	14.10	7.78	0.00	0.00	5.95
Acidified	5	27	35	14	7.42	108.01	2792.20	2782.10	3150.37	58.44	3316.8	3287.0	0.89	1.39	7.73	7.20	7.056	47.452	31.2	2397	0.00	19.62	6.85	0.00	32.11
Acidified	6	27	35	14	7.42	108.01	2792.20	2782.10	3150.37	58.44	3316.8	3287.0	0.89	1.39	6.87	6.20	5.401	37.764	23.4	2403	0.00	0.00	0.00	0.00	43.71
Acidified	7	27	35	14	7.42	108.01	2792.20	2782.10	3150.37	58.44	3316.8	3287.0	0.89	1.39	5.57	5.90	1.493	19.664	31.0	1710	0.00	0.00	4.19	0.00	5.70
Acidified	8	27	35	14	7.42	108.01	2792.20	2782.10	3150.37	58.44	3316.8	3287.0	0.89	1.39	7.24	5.00	4.703	51.432	36.8	1416	6.19	12.74	3.50	0.00	0.00
Acidified	9	27	35	14	7.42	108.01	2792.20	2782.10	3150.37	58.44	3316.8	3287.0	0.89	1.39	7.63	6.70	6.532	35.073	31.5	1686	18.80	0.00	16.10	15.67	0.95
Acidified	10	27	35	14	7.42	108.01	2792.20	2782.10	3150.37	58.44	3316.8	3287.0	0.89	1.39							0.00	0.00	12.98	11.58	2.14