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Langer, Gerald; Bode, Maya (2011): Seawater carbonate chemistry, growth rate and morphology of Calcidiscus leptoporus (RCC1135) during experiments, 2011 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.763286, Supplement to: Langer, G; Bode, M (2011): CO2 mediation of adverse effects of seawater acidification in Calcidiscus leptoporus. Geochemistry, Geophysics, Geosystems, 12(5), Q05001, https://doi.org/10.1029/2010GC003393

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Abstract:
The coccolithophore Calcidiscus leptoporus (strain RCC1135) was grown in dilute batch culture at CO2 levels ranging from ~200 to ~1600 µatm. Increasing CO2 concentration led to an increased percentage of malformed coccoliths and eventually (at ~1500 µatm CO2) to aggregation of cells. Carbonate chemistry of natural seawater was manipulated in three ways: first, addition of acid; second, addition of a HCO3/CO3 solution; and third, addition of both acid and HCO3/CO3 solution. The data set allowed the disentangling of putative effects of the different parameters of the carbonate system. It is concluded that CO2 is the parameter of the carbonate system which causes both aberrant coccolithogenesis and aggregation of cells.
Keyword(s):
Bottles or small containers/Aquaria (<20 L); Calcidiscus leptoporus; Calcification/Dissolution; Chromista; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species
Project(s):
Biological Impacts of Ocean Acidification (BIOACID)
European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)
European Project on Ocean Acidification (EPOCA)
Mediterranean Sea Acidification in a Changing Climate (MedSeA)
Ocean Acidification International Coordination Centre (OA-ICC)
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Seventh Framework Programme (FP7), grant/award no. 265103: Mediterranean Sea Acidification in a Changing Climate
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 IdentificationIDLanger, Gerald
2 Experimental treatmentExp treatLanger, Gerald
3 SalinitySalLanger, Gerald
4 Temperature, waterTemp°CLanger, GeraldConductivity meter (WTW, Weilheim, Gemany)
5 Carbon dioxideCO2µmol/kgLanger, GeraldCalculated using CO2SYS
6 Bicarbonate ion[HCO3]-µmol/kgLanger, GeraldCalculated using CO2SYS
7 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmLanger, GeraldCalculated using CO2SYS
8 Carbonate ion[CO3]2-µmol/kgLanger, GeraldCalculated using CO2SYS
9 Carbon, inorganic, dissolvedDICµmol/kgLanger, GeraldPhotometrically using autoanalyzer QUAATRO
10 Alkalinity, totalATµmol/kgLanger, GeraldAlkalinity, Gran titration (Gran, 1950)
11 pHpHLanger, GeraldConductivity meter (WTW, Weilheim, Gemany)Total scale
12 Calcite saturation stateOmega CalLanger, GeraldCalculated using CO2SYS
13 Carbon, inorganic, particulate, per cellPIC/cellpg/#Langer, GeraldCalculated, see reference(s)
14 Particulate inorganic carbon per cell, standard deviationPIC/cell std dev±Langer, Gerald
15 Carbon, organic, particulate, per cellPOC/cellpg/#Langer, GeraldGas chromatography (EURO EA Elemental Analyser, EUROVECTOR)
16 Particulate organic carbon content per cell, standard deviationPOC cont/cell std dev±Langer, Gerald
17 Particulate inorganic carbon production per cellPIC prod/cellpg/#/dayLanger, GeraldCalculated, see reference(s)
18 Particulate inorganic carbon, production, standard deviationPIC prod std dev±Langer, Gerald
19 Particulate organic carbon production per cellPOC prod/cellpg/#/dayLanger, GeraldCalculated, see reference(s)
20 Particulate organic carbon, production, standard deviationPOC prod std dev±Langer, Gerald
21 Particulate inorganic carbon/particulate organic carbon ratioPIC/POCLanger, GeraldCalculated
22 Particulate inorganic carbon/particulate organic carbon ratio, standard deviationPIC/POC ratio std dev±Langer, Gerald
23 Growth rateµ#/dayLanger, GeraldCalculated, see reference(s)
24 Growth rate, standard deviationµ std dev±Langer, Gerald
25 Calcidiscus leptoporus, morphologyC. leptoporus morph%Langer, GeraldNormal
26 Calcidiscus leptoporus, morphology, standard deviationC. leptoporus morph std dev±Langer, GeraldNormal
27 Calcidiscus leptoporus, morphologyC. leptoporus morph%Langer, GeraldMalformed
28 Calcidiscus leptoporus, morphology, standard deviationC. leptoporus morph std dev±Langer, GeraldMalformed
29 Calcidiscus leptoporus, morphologyC. leptoporus morph%Langer, GeraldIncomplete
30 Calcidiscus leptoporus, morphology, standard deviationC. leptoporus morph std dev±Langer, GeraldIncomplete
31 Calcidiscus leptoporus, morphologyC. leptoporus morph%Langer, GeraldMalformed and incomplete
32 Calcidiscus leptoporus, morphology, standard deviationC. leptoporus morph std dev±Langer, GeraldMalformed and incomplete
33 Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
34 Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
35 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
36 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
37 Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
38 Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
39 Carbon, inorganic, dissolvedDICµmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
40 Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
41 Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
246 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
ID		2 
Exp treat		3 
Sal		4 
Temp [°C]
(Conductivity meter (WTW, Weil...)		5 
CO2 [µmol/kg]
(Calculated using CO2SYS)		6 
[HCO3]- [µmol/kg]
(Calculated using CO2SYS)		7 
pCO2water_SST_wet [µatm]
(Calculated using CO2SYS)		8 
[CO3]2- [µmol/kg]
(Calculated using CO2SYS)		9 
DIC [µmol/kg]
(Photometrically using autoana...)		10 
AT [µmol/kg]
(Alkalinity, Gran titration (G...)		11 
pH
(Total scale, Conductivity met...)		12 
Omega Cal
(Calculated using CO2SYS)		13 
PIC/cell [pg/#]
(Calculated, see reference(s))		14 
PIC/cell std dev [±]		15 
POC/cell [pg/#]
(Gas chromatography (EURO EA E...)		16 
POC cont/cell std dev [±]		17 
PIC prod/cell [pg/#/day]
(Calculated, see reference(s))		18 
PIC prod std dev [±]		19 
POC prod/cell [pg/#/day]
(Calculated, see reference(s))		20 
POC prod std dev [±]		21 
PIC/POC
(Calculated)		22 
PIC/POC ratio std dev [±]		23 
µ [#/day]
(Calculated, see reference(s))		24 
µ std dev [±]		25 
C. leptoporus morph [%]
(Normal)		26 
C. leptoporus morph std dev [±]
(Normal)		27 
C. leptoporus morph [%]
(Malformed)		28 
C. leptoporus morph std dev [±]
(Malformed)		29 
C. leptoporus morph [%]
(Incomplete)		30 
C. leptoporus morph std dev [±]
(Incomplete)		31 
C. leptoporus morph [%]
(Malformed and incomplete)		32 
C. leptoporus morph std dev [±]
(Malformed and incomplete)		33 
CSC flag
(Calculated using seacarb afte...)		34 
CO2 [µmol/kg]
(Calculated using seacarb afte...)		35 
pCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		36 
fCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		37 
[HCO3]- [µmol/kg]
(Calculated using seacarb afte...)		38 
[CO3]2- [µmol/kg]
(Calculated using seacarb afte...)		39 
DIC [µmol/kg]
(Calculated using seacarb afte...)		40 
Omega Arg
(Calculated using seacarb afte...)		41 
Omega Cal
(Calculated using seacarb afte...)
Experiment 1pCO2=21532207.11879215336.3222227208.338.2291.15.959.87.163.84.138.15.01.70.20.700.0388.60.96.91.01.30.43.20.887.14217.62216.891910.15341.702259.05.398.35
Experiment 2pCO2=917322030.182399171515.09783114398.3437.00127.812.071.012.075.87.142.17.21.80.30.590.057.60.315.42.62.80.874.23.0830.26921.79918.708279.361515.569825.223.9237.03
Experiment 3pCO2=969322031.8209096993.4221623547.722.28111.04.180.03.375.92.854.72.31.40.10.680.0630.61.620.92.21.21.947.22.5832.41987.28983.982127.1993.412253.01.472.28
Experiment 4pCO2=1269322041.62084126970.2219622917.601.72100.018.365.312.563.411.842.28.11.60.70.650.0414.31.617.02.21.30.767.51.9842.561296.361292.022118.8070.582231.91.111.72
Experiment 5pCO2=1634322053.62057163453.0216322227.491.29181.920.3171.816.062.06.958.65.41.10.10.340.071.40.52.81.21.10.194.81.7854.061646.901641.392089.4654.032197.60.851.32
Experiment 6pCO2=1532322050.345711532275.0489751787.866.7287.616.1136.523.124.84.638.66.50.60.10.210.011.10.33.00.92.61.093.31.5850.451536.781531.634570.62277.054898.14.376.77