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Lannig, G et al. (2011): Seawater carbonate chemistry and biological processes of mussel Crassostrea gigas during experiments, 2011. doi:10.1594/PANGAEA.761915,
Supplement to: Lannig, Gisela; Eilers, Silke; Pörtner, Hans-Otto; Sokolova, Inna A; Bock, Christian (2010): Impact of ocean acidification on energy metabolism of oyster, Crassostrea gigas-Changes in metabolic pathways and thermal response. Marine Drugs, 8(8), 2318-2339, doi:10.3390/md8082318

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Abstract:
Climate change with increasing temperature and ocean acidification (OA) poses risks for marine ecosystems. According to Pörtner and Farrell [1], synergistic effects of elevated temperature and CO2-induced OA on energy metabolism will narrow the thermal tolerance window of marine ectothermal animals. To test this hypothesis, we investigated the effect of an acute temperature rise on energy metabolism of the oyster, Crassostrea gigas chronically exposed to elevated CO2 levels (partial pressure of CO2 in the seawater ~0.15 kPa, seawater pH ~ 7.7). Within one month of incubation at elevated PCO2 and 15 °C hemolymph pH fell (pHe = 7.1 ± 0.2 (CO2-group) vs. 7.6 ± 0.1 (control)) and PeCO2 values in hemolymph increased (0.5 ± 0.2 kPa (CO2-group) vs. 0.2 ± 0.04 kPa (control)). Slightly but significantly elevated bicarbonate concentrations in the hemolymph of CO2-incubated oysters ([HCO-3]e = 1.8 ± 0.3 mM (CO2-group) vs. 1.3 ± 0.1 mM (control)) indicate only minimal regulation of extracellular acid-base status. At the acclimation temperature of 15 °C the OA-induced decrease in pHe did not lead to metabolic depression in oysters as standard metabolism rates (SMR) of CO2-exposed oysters were similar to controls. Upon acute warming SMR rose in both groups, but displayed a stronger increase in the CO2-incubated group. Investigation in isolated gill cells revealed a similar temperature-dependence of respiration between groups. Furthermore, the fraction of cellular energy demand for ion regulation via Na+/K+-ATPase was not affected by chronic hypercapnia or temperature. Metabolic profiling using 1H-NMR spectroscopy revealed substantial changes in some tissues following OA exposure at 15 °C. In mantle tissue alanine and ATP levels decreased significantly whereas an increase in succinate levels was observed in gill tissue. These findings suggest shifts in metabolic pathways following OA-exposure. Our study confirms that OA affects energy metabolism in oysters and suggests that climate change may affect populations of sessile coastal invertebrates such as mollusks
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Experimental treatmentExp trtmLannig, Gisela
2SalinitySalLannig, Gisela
3Salinity, standard deviationSal std dev±Lannig, Gisela
4ReplicatesRepl#Lannig, Gisela
5Temperature, waterTemp°CLannig, Gisela
6pHpHLannig, GiselaMeasuredNBS scale
7pH, standard deviationpH std dev±Lannig, GiselaNBS scale
8Carbon dioxide, partial pressurepCO2PaLannig, GiselaCalculated using CO2SYS
9Carbon dioxide, partial pressure, standard deviationpCO2 std dev±Lannig, Gisela
10Bicarbonate ion[HCO3]-µmol/kgLannig, GiselaCalculated using CO2SYS
11Bicarbonate ion, standard deviation[HCO3]- std dev±Lannig, Gisela
12Calcite saturation stateOmega CalLannig, GiselaCalculated using CO2SYS
13Calcite saturation state, standard deviationOmega Cal std dev±Lannig, Gisela
14Aragonite saturation stateOmega ArgLannig, GiselaCalculated using CO2SYS
15Aragonite saturation state, standard deviationOmega Arg std dev±Lannig, Gisela
16Crassostrea gigas, haemolymph, pHC. gigas pH (ha)Lannig, Gisela
17Crassostrea gigas, haemolymph, partial pressure of carbon dioxideC. gigas pCO2 (ha)kPaLannig, GiselaGas chromatography
18Crassostrea gigas, haemolymph, partial pressure of oxygenC. gigas pO2 (ha)kPaLannig, GiselaGas chromatography
19Crassostrea gigas, haemolymph, dissolved inorganic carbonC. gigas DIC (ha)mmol/lLannig, GiselaIon chromatography
20Crassostrea gigas, haemolymph, bicarbonate ionC. gigas [HCO3]- (ha)mmol/lLannig, GiselaIon chromatography
21Crassostrea gigas, haemolymph, calcium ionC. gigas Ca2+ (ha)mmol/lLannig, GiselaIon chromatography
22Crassostrea gigas, haemolymph, sodium ionC. gigas Na+ (ha)mmol/lLannig, GiselaIon chromatography
23Crassostrea gigas, haemolymph, potassium ionC. gigas K+ (ha)mmol/lLannig, GiselaIon chromatography
24Crassostrea gigas, alanine, wet tissue massC. gigas alanineµmol/gLannig, GiselaTopSpin 2.5 (Bruker Biospin GmbH, Germany)Muscle
25Crassostrea gigas, succinate, wet tissue massC. gigas succinateµmol/gLannig, GiselaTopSpin 2.5 (Bruker Biospin GmbH, Germany)Muscle
26Crassostrea gigas, glycogen, wet tissue massC. gigas glycogenµmol/gLannig, GiselaTopSpin 2.5 (Bruker Biospin GmbH, Germany)Muscle
27Crassostrea gigas, ATP, wet tissue massC. gigas ATPµmol/gLannig, GiselaTopSpin 2.5 (Bruker Biospin GmbH, Germany)Muscle
28Crassostrea gigas, alanine, wet tissue massC. gigas alanineµmol/gLannig, GiselaTopSpin 2.5 (Bruker Biospin GmbH, Germany)Mantle
29Crassostrea gigas, succinate, wet tissue massC. gigas succinateµmol/gLannig, GiselaTopSpin 2.5 (Bruker Biospin GmbH, Germany)Mantle
30Crassostrea gigas, glycogen, wet tissue massC. gigas glycogenµmol/gLannig, GiselaTopSpin 2.5 (Bruker Biospin GmbH, Germany)Mantle
31Crassostrea gigas, ATP, wet tissue massC. gigas ATPµmol/gLannig, GiselaTopSpin 2.5 (Bruker Biospin GmbH, Germany)Mantle
32Crassostrea gigas, alanine, wet tissue massC. gigas alanineµmol/gLannig, GiselaTopSpin 2.5 (Bruker Biospin GmbH, Germany)Gills
33Crassostrea gigas, succinate, wet tissue massC. gigas succinateµmol/gLannig, GiselaTopSpin 2.5 (Bruker Biospin GmbH, Germany)Gills
34Crassostrea gigas, glycogen, wet tissue massC. gigas glycogenµmol/gLannig, GiselaTopSpin 2.5 (Bruker Biospin GmbH, Germany)Gills
35Crassostrea gigas, ATP, wet tissue massC. gigas ATPµmol/gLannig, GiselaTopSpin 2.5 (Bruker Biospin GmbH, Germany)Gills
36Condition indexCILannig, Gisela
37Metabolic rate of oxygen, standard, normalizedMet rate O2 normµmol/h/gLannig, Gisela
38Crassostrea gigas, respiration rate, oxygen, per cellC. gigas resp O2nmol/min/106#Lannig, Gisela
39Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmLannig, Gisela
40Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
41pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
42Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
43Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
44Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
45Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
46Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
47Carbon, inorganic, dissolvedDICµmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
48Alkalinity, totalATµmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
49Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
50Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
Size:
2179 data points

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