Citation:

bg-7-3915-2010

Name: Seawater carbonate chemistry and Gadus morhua length, weight and sperm biological processes, 2010
Published: 2010
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Baltic Sea; Bottles or small containers/Aquaria (<20 L); Chordata; Coast and continental shelf; Gadus morhua; Growth/Morphology; Laboratory experiment; Nekton; Pelagos; Reproduction; Single species; Temperate

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Abstract:

Ocean acidification, as a consequence of increasing marine pCO2, may have severe effects on the physiology of marine organisms. However, experimental studies remain scarce, in particular concerning fish. While adults will most likely remain relatively unaffected by changes in seawater pH, early life-history stages are potentially more sensitive - particularly the critical stage of fertilization, in which sperm motility plays a central role. In this study, the effects of ocean acidification (decrease of pHT to 7.55) on sperm motility of Baltic cod, Gadus morhua, were assessed. We found no significant effect of decreased pH on sperm speed, rate of change of direction or percent motility for the population of cod analyzed. We predict that future ocean acidification will probably not pose a problem for sperm behavior, and hence fertilization success, of Baltic cod.

Keyword(s):

Animalia; Baltic Sea; Bottles or small containers/Aquaria (<20 L); Chordata; Coast and continental shelf; Gadus morhua; Growth/Morphology; Laboratory experiment; Nekton; Pelagos; Reproduction; Single species; Temperate

Project(s):

Biological Impacts of Ocean Acidification (BIOACID)

European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)

European Project on Ocean Acidification (EPOCA)

Ocean Acidification International Coordination Centre (OA-ICC)

Funding:

Seventh Framework Programme (FP7), grant/award no. 211384 : European Project on Ocean Acidification

Sixth Framework Programme (FP6), grant/award no. 511106 : European network of excellence for Ocean Ecosystems Analysis

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Identification	ID		Frommel, Andrea Y
2	Experimental treatment	Exp treat		Frommel, Andrea Y
3	Salinity	Sal		Frommel, Andrea Y	Measured
4	Temperature, water	Temp	°C	Frommel, Andrea Y	Measured
5	Alkalinity, total	AT	µmol/kg	Frommel, Andrea Y	Measured
6	Carbon, inorganic, dissolved	DIC	µmol/kg	Frommel, Andrea Y	Measured
7	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Frommel, Andrea Y	Calculated using CO2SYS
8	pH	pH		Frommel, Andrea Y	Calculated using CO2SYS	Total scale
9	Gadus morhua, standard length	G. morhua sl	mm	Frommel, Andrea Y	Measured
10	Gadus morhua, mass	G. morhua m	g	Frommel, Andrea Y	Measured
11	Gadus morhua, sperm, swimming speed	G. morhua sperm speed	µm/s	Frommel, Andrea Y	Measured	Mean
12	Gadus morhua, sperm, swimming speed	G. morhua sperm speed	µm/s	Frommel, Andrea Y	Measured	Moving >25 µm/s
13	Gadus morhua, sperm, swimming speed	G. morhua sperm speed	µm/s	Frommel, Andrea Y	Measured	Moving >50 µm/s
14	Gadus morhua, sperm, motile	G. morhua sperm motlie	%	Frommel, Andrea Y	Measured	Moving >25 µm/s
15	Gadus morhua, sperm, motile	G. morhua sperm motlie	%	Frommel, Andrea Y	Measured	Moving >50 µm/s
16	Carbonate system computation flag	CSC flag		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
17	pH	pH		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)	Total scale
18	Carbon dioxide	CO2	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
19	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
20	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
21	Bicarbonate ion	[HCO3]-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
22	Carbonate ion	[CO3]2-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
23	Aragonite saturation state	Omega Arg		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
24	Calcite saturation state	Omega Cal		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

4200 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 ID	2 Exp treat	3 Sal (Measured)	4 Temp [°C] (Measured)	5 AT [µmol/kg] (Measured)	6 DIC [µmol/kg] (Measured)	7 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	8 pH (Total scale, Calculated using...)	9 G. morhua sl [mm] (Measured)	10 G. morhua m [g] (Measured)	11 G. morhua sperm speed [µm/s] (Mean, Measured)	12 G. morhua sperm speed [µm/s] (Moving >25 µm/s, Measured)	13 G. morhua sperm speed [µm/s] (Moving >50 µm/s, Measured)	14 G. morhua sperm motlie [%] (Moving >25 µm/s, Measured)	15 G. morhua sperm motlie [%] (Moving >50 µm/s, Measured)	16 CSC flag (Calculated using seacarb afte...)	17 pH (Total scale, Calculated using...)	18 CO2 [µmol/kg] (Calculated using seacarb afte...)	19 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	20 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	21 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	22 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	23 Omega Arg (Calculated using seacarb afte...)	24 Omega Cal (Calculated using seacarb afte...)
M15-1	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	410	356	45.95	64.78	85.10	61.54	35.38	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M15-2	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	410	356	87.60	100.17	120.13	85.19	64.20	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M15-3	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	410	356	69.89	85.13	102.98	77.56	57.05	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M15-4	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	410	356	70.76	84.15	100.02	80.46	59.77	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M15-5	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	410	356	59.83	75.07	92.42	74.11	50.89	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M15-1	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	410	356	73.25	84.24	105.13	83.45	58.27	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M15-2	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	410	356	56.32	72.11	92.61	72.25	46.07	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M15-3	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	410	356	70.62	81.60	108.30	83.44	52.98	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M15-4	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	410	356	34.54	60.59	85.36	43.73	22.37	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M15-5	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	410	356	62.00	76.71	97.20	76.06	49.77	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M16-1	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	370	382	71.96	101.25	1099.94	87.83	63.49	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M16-2	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	370	382	62.22	96.52	1099.94	85.94	54.69	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M16-3	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	370	382	55.43	96.03	1070.74	83.14	45.93	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M16-4	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	370	382	67.25	105.39	1146.27	82.20	55.08	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M16-5	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	370	382	80.83	108.45	1060.40	90.29	68.57	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M16-1	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	370	382	88.54	118.08	1079.23	92.67	69.33	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M16-2	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	370	382	61.16	96.65	929.57	80.24	54.49	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M16-3	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	370	382	64.64	108.86	798.08	74.03	51.95	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M16-4	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	370	382	60.11	102.60	848.83	75.86	49.14	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M16-5	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	370	382	67.87	99.35	1133.10	85.35	59.87	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M17-1	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	470	779	38.47	75.84	1028.27	74.87	34.17	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M17-2	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	470	779	32.10	66.82	1028.27	67.16	28.86	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M17-3	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	470	779	27.99	66.05	867.46	59.43	23.36	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M17-4	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	470	779	40.81	75.57	1038.86	72.56	38.14	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M17-5	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	470	779	48.06	89.59	1193.73	81.33	39.42	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M17-1	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	470	779	30.75	75.15	893.69	58.30	24.70	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M17-2	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	470	779	37.14	75.69	1152.85	72.70	32.57	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M17-3	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	470	779	38.54	79.90	1128.96	79.19	29.95	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M17-4	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	470	779	31.66	74.63	985.81	62.66	25.32	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M17-5	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	470	779	34.75	70.84	1209.02	81.95	28.20	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M18-1	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	460	782	45.04	97.33	789.98	68.12	34.06	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M18-2	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	460	782	66.17	99.64	789.98	82.58	58.33	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M18-3	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	460	782	44.17	87.25	1005.26	71.96	37.38	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M18-4	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	460	782	49.48	85.60	873.30	69.03	47.10	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M18-5	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	460	782	43.69	89.71	1055.40	80.49	34.15	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M18-1	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	460	782	46.08	95.57	924.70	72.65	35.90	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M18-2	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	460	782	65.02	98.73	1114.75	85.19	56.79	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M18-3	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	460	782	67.97	111.60	931.75	81.16	52.17	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M18-4	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	460	782	25.45	68.95	950.88	56.82	17.05	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M18-5	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	460	782	55.02	93.79	1049.30	80.63	48.17	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M19-1	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	390	429	59.48	87.34	1237.47	88.83	55.84	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M19-2	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	390	429	56.97	109.45	1237.47	78.33	42.22	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M19-3	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	390	429	42.62	90.11	1108.71	69.63	34.07	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M19-4	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	390	429	49.62	85.06	1170.22	82.81	44.80	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M19-5	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	390	429	51.29	90.49	1187.11	82.58	42.13	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M19-1	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	390	429	44.75	91.28	1169.99	75.00	34.09	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M19-2	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	390	429	30.33	68.57	965.00	66.46	23.42	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M19-3	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	390	429	43.24	79.86	1126.72	81.82	38.18	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M19-4	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	390	429	43.60	78.67	1098.17	84.13	37.30	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M20-1	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	380	445	39.99	68.71	930.10	77.69	40.50	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M20-2	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	380	445	45.45	75.35	930.10	79.87	45.91	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M20-3	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	380	445	43.39	68.22	1298.15	84.50	47.50	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M20-4	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	380	445	39.84	79.22	1075.65	73.33	33.33	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M20-5	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	380	445	45.91	79.28	1298.23	82.30	42.58	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M20-1	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	380	445	46.60	81.66	1033.37	74.73	43.96	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M20-3	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	380	445	45.22	77.61	1287.64	83.80	42.59	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M20-4	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	380	445	51.82	82.96	1166.22	88.12	48.51	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M20-5	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	380	445	39.91	68.74	1317.78	79.91	38.86	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M22-1	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	400	541	48.34	94.46	999.75	67.80	40.68	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M22-2	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	400	541	43.37	76.45	1186.65	80.22	41.21	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M22-3	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	400	541	61.19	98.45	1064.45	84.21	52.63	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M22-4	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	400	541	58.02	91.51	1151.42	80.20	53.81	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M22-5	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	400	541	67.04	107.94	938.55	81.82	53.48	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M22-2	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	400	541	65.08	96.69	1282.06	84.95	58.60	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M22-3	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	400	541	65.33	99.82	1061.79	83.42	56.68	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M22-4	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	400	541	63.97	101.14	1292.10	87.15	52.51	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M22-5	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	400	541	54.74	99.45	1063.73	76.44	43.98	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M23-1	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	400	533	50.76	86.12	889.91	69.31	49.50	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M23-3	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	400	533	76.77	121.29	-53.28	73.28	58.02	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M23-4	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	400	533	70.51	123.18	977.51	78.02	49.45	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M23-5	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	400	533	54.11	88.21	1147.36	75.28	51.69	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M23-1	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	400	533	79.12	118.51	805.35	76.56	61.72	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M23-2	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	400	533	62.69	112.96	968.85	74.07	48.15	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M23-3	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	400	533	51.72	89.60	1159.60	75.47	47.17	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M23-4	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	400	533	71.58	112.76	1085.87	80.70	56.14	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M23-5	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	400	533	59.73	103.29	784.67	68.67	50.60	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M24-1	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	460	887	65.08	112.25	1061.11	75.76	49.49	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M24-2	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	460	887	89.93	133.31	866.47	75.86	63.79	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M24-3	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	460	887	85.68	115.81	1221.53	88.89	68.25	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M24-4	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	460	887	57.71	108.78	1050.12	72.53	43.96	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M24-5	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	460	887	67.86	111.47	1030.64	77.66	53.19	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M24-1	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	460	887	76.90	110.14	1030.30	80.47	64.06	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M24-2	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	460	887	75.81	120.77	929.68	76.52	56.82	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M24-3	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	460	887	68.71	108.20	1252.76	86.09	54.78	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M24-4	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	460	887	53.34	92.96	921.26	76.19	45.71	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M24-5	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	460	887	76.18	114.76	1030.83	81.30	60.16	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M26-1	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	410	511	40.83	84.69	1315.32	75.42	32.40	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M26-2	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	410	511	52.43	83.44	1391.00	82.29	51.04	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M26-3	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	410	511	49.87	82.67	1203.36	82.89	46.52	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M26-4	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	410	511	39.64	73.84	1141.18	71.89	37.30	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M26-5	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	410	511	41.56	80.17	1128.90	71.56	37.78	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M26-1	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	410	511	44.15	80.42	1098.88	73.50	41.88	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M26-2	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	410	511	35.68	73.39	1121.68	64.22	33.19	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M26-3	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	410	511	46.48	83.84	1008.89	69.51	43.50	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M26-4	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	410	511	40.26	76.16	1346.45	76.58	36.49	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M26-5	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	410	511	33.27	74.42	1074.94	61.30	29.13	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M27-1	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	410	636	70.04	104.38	986.27	83.66	59.48	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M27-2	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	410	636	56.84	83.98	1342.78	83.24	57.30	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M27-3	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	410	636	62.36	97.77	1517.66	89.00	52.63	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M27-4	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	410	636	56.86	91.51	1193.71	80.14	52.05	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M27-5	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	410	636	58.43	89.55	1333.27	89.89	53.19	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M27-1	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	410	636	62.63	89.45	1045.50	87.10	60.65	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M27-2	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	410	636	51.93	86.46	1268.17	78.46	47.69	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M27-3	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	410	636	58.07	88.38	1345.97	87.06	54.12	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M27-4	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	410	636	47.46	82.60	1045.29	76.88	45.09	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M27-5	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	410	636	54.48	86.58	1229.14	83.49	51.42	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M28-1	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	430	571	86.93	121.92	990.98	84.15	65.85	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M28-2	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	430	571	67.86	103.80	1333.40	85.48	56.45	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M28-3	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	430	571	60.02	95.53	1194.58	82.32	53.59	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M28-4	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	430	571	60.04	103.36	1087.86	80.27	48.30	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M28-5	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	430	571	68.76	98.15	1195.65	85.71	61.90	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M28-1	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	430	571	59.43	111.10	953.07	73.86	44.44	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M28-2	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	430	571	63.17	104.10	1216.73	80.66	51.93	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M28-3	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	430	571	61.30	108.82	938.36	73.10	48.28	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M28-4	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	430	571	71.26	114.52	1067.06	81.82	54.55	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M28-5	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	430	571	53.63	99.31	1153.97	74.29	43.33	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M29-1	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	400	550	74.25	102.00	1087.44	89.22	65.87	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M29-2	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	400	550	70.37	97.53	1190.55	86.41	64.67	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M29-3	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	400	550	61.90	89.96	1189.93	87.71	58.10	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M29-4	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	400	550	62.24	101.02	1141.93	83.12	52.60	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M29-5	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	400	550	50.66	82.02	1130.98	81.58	48.42	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M29-1	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	400	550	81.70	102.90	1058.37	95.15	72.12	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M29-2	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	400	550	62.03	85.98	1450.27	91.67	61.11	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M29-3	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	400	550	53.68	81.27	1092.29	84.80	54.40	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M29-4	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	400	550	60.42	89.89	1260.12	88.15	56.30	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M29-5	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	400	550	52.25	79.73	1330.05	89.39	50.51	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M31-1	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	480	759	48.30	80.87	1078.44	73.33	49.17	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M31-2	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	480	759	68.11	110.56	1053.43	80.91	53.64	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M31-3	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	480	759	54.58	88.63	1301.49	84.14	50.34	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M31-4	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	480	759	64.50	88.58	1120.83	87.69	63.85	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M31-5	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	480	759	47.26	81.07	1358.40	80.25	45.22	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M31-1	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	480	759	83.27	110.74	1135.87	84.95	69.89	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M31-2	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	480	759	58.06	91.79	1087.40	77.06	54.12	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M31-3	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	480	759	65.60	95.75	1255.28	89.05	59.20	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M31-4	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	480	759	53.25	84.39	1073.57	76.11	53.10	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M31-5	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	480	759	51.30	85.30	1261.04	81.94	47.22	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M32-1	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	480	809	57.30	85.98	1251.84	85.71	55.71	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M32-2	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	480	809	63.94	92.33	1393.61	90.73	59.02	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M32-3	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	480	809	55.14	83.66	1356.66	88.48	53.93	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M32-4	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	480	809	46.74	79.29	1227.77	82.73	45.00	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M32-5	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	480	809	57.39	84.68	1463.52	89.81	55.34	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M32-1	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	480	809	55.11	85.57	1190.37	84.40	52.29	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M32-2	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	480	809	43.03	70.64	1262.42	80.75	43.93	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M32-3	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	480	809	54.98	85.79	1300.44	85.24	51.43	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M32-4	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	480	809	46.42	76.13	1171.35	83.02	45.28	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M32-5	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	480	809	27.60	113.17	1318.31	86.44	54.62	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M33-1	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	400	462	98.34	117.34	1213.17	95.89	78.77	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M33-2	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	400	462	94.48	114.22	1205.78	94.67	78.00	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M33-3	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	400	462	86.63	109.58	1134.09	91.72	73.79	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M33-4	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	400	462	82.89	105.51	1162.20	86.91	74.35	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M33-5	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	400	462	81.23	111.75	1325.12	91.15	66.67	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M33-1	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	400	462	84.35	111.28	1254.04	90.59	69.41	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M33-2	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	400	462	109.08	122.97	1135.85	95.59	86.03	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M33-3	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	400	462	79.15	118.19	1043.33	83.63	60.23	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M33-4	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	400	462	65.57	101.36	1063.71	83.22	55.94	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M33-5	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	400	462	90.62	113.55	1039.21	90.77	75.38	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M35-1	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	500	991	42.21	82.63	1272.27	78.17	34.50	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M35-2	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	500	991	58.68	82.57	1230.42	85.39	61.24	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M35-3	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	500	991	54.69	85.75	1479.53	88.21	50.77	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M35-4	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	500	991	50.79	77.89	1252.28	84.12	52.36	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M35-1	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	500	991	52.03	82.27	1297.20	82.42	50.00	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M35-2	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	500	991	52.93	87.85	1365.69	85.25	47.00	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M35-3	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	500	991	52.12	81.31	1193.37	81.30	52.44	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M35-4	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	500	991	42.61	77.41	1057.00	69.73	41.62	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M35-5	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	500	991	48.81	79.27	1335.47	81.00	47.50	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M36-1	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	410	537	58.63	90.03	1237.15	86.63	53.96	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M36-2	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	410	537	65.58	89.94	1428.37	89.41	64.12	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M36-3	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	410	537	42.32	77.64	1234.66	79.27	38.86	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M36-4	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	410	537	46.27	87.88	1200.96	80.71	37.56	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M36-5	Control	17.4	9.4	2068.8	1973.4	390.8	8.08	410	537	63.37	86.38	1247.19	92.56	62.79	15	8.09	18.78	380.50	379.04	1868.93	85.69	1.32	2.24
M36-1	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	410	537	58.15	90.90	1134.01	82.35	52.94	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M36-2	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	410	537	58.53	88.08	1273.58	88.78	55.12	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M36-3	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	410	537	64.36	92.61	1224.84	86.02	60.22	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M36-4	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	410	537	62.11	86.13	1246.53	89.60	62.43	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70
M36-5	Acidified	17.4	9.4	2024.4	2055.2	1364.8	7.56	410	537	54.39	83.75	1232.88	85.64	52.82	15	7.57	66.64	1350.20	1345.03	1961.94	26.61	0.41	0.70