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Crawley, Alicia; Kline, David I; Dunn, Simon; Anthony, Kenneth R N; Dove, Sophie (2010): Seawater carbonate chemistry and dark respiration and photosynthetic capacity during experiments with coral Acropora formosa, 2010 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.755151, Supplement to: Crawley, A et al. (2010): The effect of ocean acidification on symbiont photorespiration and productivity in Acropora formosa. Global Change Biology, 16(2), 851-863, https://doi.org/10.1111/j.1365-2486.2009.01943.x

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Abstract:
Ocean acidification is expected to lower the net accretion of coral reefs yet little is known about its effect on coral photophysiology. This study investigated the effect of increasing CO2 on photosynthetic capacity and photoprotection in Acropora formosa. The photoprotective role of photorespiration within dinoflagellates (genus Symbiodinium) has largely been overlooked due to focus on the presence of a carbon-concentrating mechanism despite the evolutionary persistence of a Form II Rubisco. The photorespiratory fixation of oxygen produces phosphoglycolate that would otherwise inhibit carbon fixation though the Calvin cycle if it were not converted to glycolate by phosphoglycolate phosphatase (PGPase). Glycolate is then either excreted or dealt with by enzymes in the photorespiratory glycolate and/or glycerate pathways adding to the pool of carbon fixed in photosynthesis. We found that CO2 enrichment led to enhanced photoacclimation (increased chlorophyll a per cell) to the subsaturating light levels. Light-enhanced dark respiration per cell and xanthophyll de-epoxidation increased, with resultant decreases in photosynthetic capacity (Pnmax) per chlorophyll. The conservative CO2 emission scenario (A1B; 600-790 ppm) led to a 38% increase in the Pnmax per cell whereas the 'business-as-usual' scenario (A1F1; 1160-1500 ppm) led to a 45% reduction in PGPase expression and no change in Pnmax per cell. These findings support an important functional role for PGPase in dinoflagellates that is potentially compromised under CO2 enrichment.
Keyword(s):
Acropora formosa; Animalia; Benthic animals; Benthos; Cnidaria; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Primary production/Photosynthesis; Respiration; Single species; South Pacific; Tropical
Project(s):
European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)
European Project on Ocean Acidification (EPOCA)
Ocean Acidification International Coordination Centre (OA-ICC)
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 SalinitySalCrawley, Alicia
2 Temperature, waterTemp°CCrawley, Alicia
3 Light:Dark cycleL:Dhh:hhCrawley, AliciaMeasured
4 Radiation, photosynthetically activePARµmol/m2/sCrawley, AliciaOdyssey light loggers (Dataflow Systems, Christchurch, New Zealand)
5 pHpHCrawley, AliciaNBS scale
6 Alkalinity, totalATµmol/kgCrawley, AliciaT50 Titrator (Mettler Toledo, Port Melbourne, Australia)
7 Alkalinity, total, standard deviationAT std dev±Crawley, Alicia
8 Carbon, inorganic, dissolvedDICµmol/kgCrawley, AliciaCalculated using CO2SYS
9 Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
10 pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
11 Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
12 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
13 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
14 Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
15 Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
16 Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
17 Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
18 Photosynthetic capacity, oxygen productionPnmax O2mol/mol/hCrawley, AliciaDetermined by the greatest rate of oxygen evolution at high light
19 Photosynthetic capacity, oxygen production per cellPnmax O2/cellpmol/#/hCrawley, AliciaDetermined by the greatest rate of oxygen evolution at high light
20 Respiration rate, oxygen, dark per cellResp O2 d/cellpmol/#/hCrawley, AliciaEstimated by regressing O2 against time
21 Respiration rate, oxygen, dark per cellResp O2 d/cellpmol/#/hCrawley, AliciaEstimated by regressing O2 against timeLight enhanced
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
63 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
Sal		2 
Temp [°C]		3 
L:D [hh:hh]
(Measured)		4 
PAR [µmol/m2/s]
(Odyssey light loggers (Datafl...)		5 
pH
(NBS scale)		6 
AT [µmol/kg]
(T50 Titrator (Mettler Toledo,...)		7 
AT std dev [±]		8 
DIC [µmol/kg]
(Calculated using CO2SYS)		9 
CSC flag
(Calculated using seacarb afte...)		10 
pH
(Total scale, Calculated using...)		11 
CO2 [µmol/kg]
(Calculated using seacarb afte...)		12 
pCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		13 
fCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		14 
[HCO3]- [µmol/kg]
(Calculated using seacarb afte...)		15 
[CO3]2- [µmol/kg]
(Calculated using seacarb afte...)		16 
Omega Arg
(Calculated using seacarb afte...)		17 
Omega Cal
(Calculated using seacarb afte...)		18 
Pnmax O2 [mol/mol/h]
(Determined by the greatest ra...)		19 
Pnmax O2/cell [pmol/#/h]
(Determined by the greatest ra...)		20 
Resp O2 d/cell [pmol/#/h]
(Estimated by regressing O2 ag...)		21 
Resp O2 d/cell [pmol/#/h]
(Light enhanced, Estimated by ...)
352312:121108.10241252163.5267.9715.46518.89517.211964.53183.512.884.40178.40.696-0.252-0.383
352312:121107.85241382281.7267.7229.951005.181001.942139.40112.351.772.7073.50.994-0.307-0.540
352312:121107.602422152382.7267.4756.251887.831881.742259.7266.741.051.6011.00.799-0.323-0.674